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stem-loop reverse transcription primers  (Thermo Fisher)


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    Structured Review

    Thermo Fisher stem-loop reverse transcription primers
    Stem Loop Reverse Transcription Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stem-loop+reverse+transcript+primer/stem+loop+rt+primer/pm37871404-68-10-14
    Average 90 stars, based on 1 article reviews
    stem-loop reverse transcription primers - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively.

    Article Title: Lipopolymer mediated siRNA delivery targeting aberrant oncogenes for effective therapy of myeloid leukemia in preclinical animal models.
    Article Snippet: The clinical development of tyrosine kinase inhibitors (TKI) has led to great strides in improving the survival of chronic myeloid leukemia (CML) and acute myeloid leukemia (AML) patients.. But even the new generation TKIs are rendered futile in the face of evolving landscape of acquired mutations leading to drug resistance, necessitating the pursuit of alternative therapeutic approaches.. In contrast to exploiting proteins as targets like most conventional drugs and TKIs, RNA Interference (RNAi) exerts its therapeutic action towards disease-driving aberrant genes.

    Article Title: MicroRNAs as novel biomarkers and potential therapeutic options for inflammatory cardiomyopathy
    Article Snippet: Total RNA including miRNA fraction was initially reversely transcribed to cDNA using Megaplex stem‐loop RT primer (Thermo Fisher Scientific, Inc.) for Human Pools A and B in combination with the TaqMan MicroRNA Reverse Transcription Kit (Thermo Fisher Scientific, Inc.) for low content samples.

    Article Title: Correlation and predictive modeling of serum exosomal miRNAs and serological biomarkers for lymph node metastasis in gastric cancer
    Article Snippet: Reverse transcription was performed with the TaqManTM MicroRNA Reverse Transcription Kit and specific stem-loop primers (Applied Biosystems, Thermo Fisher Scientific, USA).

    Article Title: RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation.
    Article Snippet: 10 ng of total RNA was reverse-transcribed using specific stem-loop reverse transcription primers (ThermoFisher) and miR-125b-5p levels were measured on an Eppendorf Realplex Mastercyler.

    Article Title: MicroRNAs as novel biomarkers and potential therapeutic options for inflammatory cardiomyopathy.
    Article Snippet: MicroRNA reverse transcription, preamplification, and expression analysis Total RNA including miRNA fraction was initially reversely transcribed to cDNA using Megaplex stem-loop RT primer (Thermo Fisher Scientific, Inc.) for Human Pools A and B in combination with the TaqMan MicroRNA Reverse Transcription Kit (Thermo Fisher Scientific, Inc.) for low content samples.

    Article Title: Discovery of miRNAs unique to actively transcribed erythroparvovirus infection in heart failure patients
    Article Snippet: Total RNA including miRNA fraction was initially reversely transcribed to cDNA using Megaplex stem‐loop RT primer (Thermo Fisher Scientific, Waltham, MA, USA) for Human Pool A and B in combination with the TaqMan MicroRNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA) for low content samples.

    Expressing:

    Article Title: The clinical predictive value and regulation mechanism of microRNA-188-5p in contrast-induced acute kidney injury.
    Article Snippet: Contrast-induced acute kidney injury (CI-AKI), also known as contrast-induced nephropathy (CIN), has become the third leading cause of iatrogenic AKI.. Serum creatinine (Scr) is currently used in CIN clinical diagnosis.. Patients with increased Scr have developed severe kidney injury, so there is an urgent need to find a bio-marker for CIN early diagnosis.

    Reverse Transcription:

    Article Title: The clinical predictive value and regulation mechanism of microRNA-188-5p in contrast-induced acute kidney injury.
    Article Snippet: Contrast-induced acute kidney injury (CI-AKI), also known as contrast-induced nephropathy (CIN), has become the third leading cause of iatrogenic AKI.. Serum creatinine (Scr) is currently used in CIN clinical diagnosis.. Patients with increased Scr have developed severe kidney injury, so there is an urgent need to find a bio-marker for CIN early diagnosis.



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