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genome dna  (New England Biolabs)


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  • 99

    Structured Review

    New England Biolabs genome dna
    Genome Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/statistical+program+stata+version+12/BamHI/us08039595-1386-17-31
    Average 99 stars, based on 13774 article reviews
    genome dna - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: In vivo DNA damage protection during cell migration across confining embryonic tissue environments.
    Article Snippet: mApple-53BP1 and BAF-mCherry were subcloned into a pCS2+ vector for mRNA synthesis from Addgene plasmids 69531 ref. 58 and 172447 (ref. 100). .. For mApple-53BP1, pCS2+ was digested with BamHI and EcoRI (New England Biolabs), and mApple-53BP1 was amplified with the following primers: 5′-CATCATGGATCCatggtgagcaagggcgag-3′ and 5′-GAGGAGGAATTCctacccggtagaattatc-3′. .. Fragments were ligated using a T4 DNA ligase (Promega) and transformed into competent cells.

    Article Title: SF3B1 mutation generates novel peptides from long non-coding RNAs
    Article Snippet: .. To generate a ZNF609 peptide overexpression plasmid, a DNA fragment (Twist Bioscience) containing a 3xFLAG-ZNF609-ORF sequence was amplified using Q5 polymerase (New England Biolabs) and cloned into pLVX-IRES-ZsGreen (TakaraBio) between SpeI and BamHI (New England Biolabs). .. For Cas9-related experiments, sgRNAs were designed using CRISPR-Cas9 guide RNA design tool (Integrated DNA Technologies) and cloned into pLKO.5 Puro-2A-RFP using Golden Gate cloning.

    Plasmid Preparation:

    Article Title: Zika virus produces two isoforms of the NS2A protein that play mutually exclusive roles in viral replication and virion assembly.
    Article Snippet: NS2A (E103A, N130A, K192A, K193A, Δ194–225) or S1D NS2B mutants were generated in the subclone using reverse PCR (S2 Table) with Q5 polymerase (NEB). .. Subcloned fragments were digested using KpnI and BamHI (NEB) and ligated back into the pIRO-Z backbone vector using T4 DNA ligase (NEB). ..

    Article Title: Next‐Generation Sequencing to Identify Genetic Causes of Chinese Patients Affected With Non‐retinal Developmental Eye Disorders
    Article Snippet: .. Plasmid pSPL3 served as the plasmid backbone and was digested using two restriction enzymes, XhoI and BamHI (New England Biolabs, Ipswich, Massachusetts, USA). ..

    Article Title: Reactivation of a loss-of-function SlGLK2 allele by frame-restoring genome editing in tomato
    Article Snippet: .. The P19 expression cassette was subsequently PCR-amplified and inserted into the BamH I and Hind III sites of the helper plasmid pSoup (Hellens et al. ) using the NEBuilder HiFi DNA Assembly Master Mix, resulting in pSoup-P19_K. ..

    Article Title: DNAJC17 regulates RNA splicing in auditory hair cells and is required for hearing.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: SF3B1 mutation generates novel peptides from long non-coding RNAs
    Article Snippet: .. To generate a ZNF609 peptide overexpression plasmid, a DNA fragment (Twist Bioscience) containing a 3xFLAG-ZNF609-ORF sequence was amplified using Q5 polymerase (New England Biolabs) and cloned into pLVX-IRES-ZsGreen (TakaraBio) between SpeI and BamHI (New England Biolabs). .. For Cas9-related experiments, sgRNAs were designed using CRISPR-Cas9 guide RNA design tool (Integrated DNA Technologies) and cloned into pLKO.5 Puro-2A-RFP using Golden Gate cloning.

    Expressing:

    Article Title: Reactivation of a loss-of-function SlGLK2 allele by frame-restoring genome editing in tomato
    Article Snippet: .. The P19 expression cassette was subsequently PCR-amplified and inserted into the BamH I and Hind III sites of the helper plasmid pSoup (Hellens et al. ) using the NEBuilder HiFi DNA Assembly Master Mix, resulting in pSoup-P19_K. ..

    Polymerase Chain Reaction:

    Article Title: Reactivation of a loss-of-function SlGLK2 allele by frame-restoring genome editing in tomato
    Article Snippet: .. The P19 expression cassette was subsequently PCR-amplified and inserted into the BamH I and Hind III sites of the helper plasmid pSoup (Hellens et al. ) using the NEBuilder HiFi DNA Assembly Master Mix, resulting in pSoup-P19_K. ..

    Variant Assay:

    Article Title: DNAJC17 regulates RNA splicing in auditory hair cells and is required for hearing.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Hemagglutination Assay:

    Article Title: DNAJC17 regulates RNA splicing in auditory hair cells and is required for hearing.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Clone Assay:

    Article Title: DNAJC17 regulates RNA splicing in auditory hair cells and is required for hearing.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: SF3B1 mutation generates novel peptides from long non-coding RNAs
    Article Snippet: .. To generate a ZNF609 peptide overexpression plasmid, a DNA fragment (Twist Bioscience) containing a 3xFLAG-ZNF609-ORF sequence was amplified using Q5 polymerase (New England Biolabs) and cloned into pLVX-IRES-ZsGreen (TakaraBio) between SpeI and BamHI (New England Biolabs). .. For Cas9-related experiments, sgRNAs were designed using CRISPR-Cas9 guide RNA design tool (Integrated DNA Technologies) and cloned into pLKO.5 Puro-2A-RFP using Golden Gate cloning.

    Restriction Assay:

    Article Title: Rational attenuation of elapid long-chain neurotoxin by targeted residue substitution and disulfide architecture simplification: implications for receptor interaction and immune recognition.
    Article Snippet: Elapid long-chain neurotoxins (LNTxs) exert their toxicity through high-affinity binding to nicotinic acetylcholine receptors (nAChRs).. Specific residues mediate this process, including basic (Lys23, Arg33, Arg36, Lys49), structural (Cys26, Cys30), and aromatic (Phe29) amino acids.. To investigate the functional and immunogenic implications of modifying these residues, we designed two recombinant variants of a consensus LNTx.

    Over Expression:

    Article Title: SF3B1 mutation generates novel peptides from long non-coding RNAs
    Article Snippet: .. To generate a ZNF609 peptide overexpression plasmid, a DNA fragment (Twist Bioscience) containing a 3xFLAG-ZNF609-ORF sequence was amplified using Q5 polymerase (New England Biolabs) and cloned into pLVX-IRES-ZsGreen (TakaraBio) between SpeI and BamHI (New England Biolabs). .. For Cas9-related experiments, sgRNAs were designed using CRISPR-Cas9 guide RNA design tool (Integrated DNA Technologies) and cloned into pLKO.5 Puro-2A-RFP using Golden Gate cloning.

    Sequencing:

    Article Title: SF3B1 mutation generates novel peptides from long non-coding RNAs
    Article Snippet: .. To generate a ZNF609 peptide overexpression plasmid, a DNA fragment (Twist Bioscience) containing a 3xFLAG-ZNF609-ORF sequence was amplified using Q5 polymerase (New England Biolabs) and cloned into pLVX-IRES-ZsGreen (TakaraBio) between SpeI and BamHI (New England Biolabs). .. For Cas9-related experiments, sgRNAs were designed using CRISPR-Cas9 guide RNA design tool (Integrated DNA Technologies) and cloned into pLKO.5 Puro-2A-RFP using Golden Gate cloning.



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