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bst dna polymerase (New England Biolabs)

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New England Biolabs bst dna polymerase
Bst Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/statistical+program+stata+version+12/Bst+DNA+Polymerase%2C+Full+Length/pm31317385-75-24-27
Average 97 stars, based on 1379 article reviews

bst dna polymerase - by Bioz Stars, 2026-11

97/100 stars

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Purification:

Article Title: Phylogeny of peas (Pisum sativum L.) reconstructed from genotyping-by-sequencing data highlights discordant evolution of nuclear and organellar genomes.
Article Snippet: Post-ligation clean-up has been performed using 48 μl of VAHTS DNA Clean Beads (Vazyme, China), DNA was eluted in 20 μl of dH2O. .. Following adapter fill-in with BST polymerase (NEB, USA), DNA was purified and eluted in 20 μl dH2O. .. PCR amplification was conducted in 30 μl reactions containing 7 μl of purified library DNA, 200 pM indexed PCR primer and P5 PCR primer (Wendler et al., 2014), 1\2V of Phusion High Fidelity PCR Master Mix with HF Buffer (NEB, USA).

Synthesized:

Article Title: Selective rolling circle amplification detection of site-specific DNA 5-hydroxymethylcytosine
Article Snippet: .. HiFi Taq DNA Ligase and Bst DNA Polymerase (Large Fragment) were purchased from New England Biolabs Ltd. All oligonucleotide sequences were synthesized by Sangon (Shanghai, China), with details listed in . ..

Amplification:

Article Title: Rapid LAMP-Based Detection of Mixed Begomovirus Infections in Field-Grown Tomato Plants
Article Snippet: .. LAMP reactions were performed in 12.5 μL volumes containing: 0.2 μM each of F3 and B3, 1.6 μM each of FIP, BIP, F2, and F1c, 0.8 μM each of B2 and B1c, 1.4 mM dNTPs (Thermo Fisher Scientific, Waltham, MA, USA), 8 mM MgSO4, 1X isothermal amplification buffer, 8 U Bst DNA polymerase (New England Biolabs, Ipswich, MA, USA), and 100 ng of total plant DNA. .. Parallel assays were performed with 1X WarmStart Colorimetric LAMP Master Mix (New England Biolabs) containing phenol red. https://doi.org/10.3390/v18010019 Reactions were incubated at 65 ◦C for 60 min and terminated at 80 ◦C for 5 min. Products were analyzed by electrophoresis on 2% agarose gels stained with ethidium bromide.