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m0536s dna clean concentrator 5 zymo research  (New England Biolabs)


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    New England Biolabs m0536s dna clean concentrator 5 zymo research
    M0536s Dna Clean Concentrator 5 Zymo Research, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stat+flex+software+program/Phusion+Hot+Start+Flex+Master+Mix/pm36996812-207-67-63
    Average 99 stars, based on 454 article reviews
    m0536s dna clean concentrator 5 zymo research - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Defective Atg16l1 in intestinal epithelial cells links to altered fecal microbiota and metabolic shifts during pregnancy in mice.
    Article Snippet: .. Each primer contained additional sequences for a 12 base Golay barcode, an Illumina adaptor, and a linker sequence.35 PCR was performed using the Phusion Hot Start Flex 2X Master Mix (NEB) in a GeneAmp PCR system 9700 (Applied Biosystems) and the following program [98°C for 3 min, 25× (98°C for 20 s, 55°C for 30 s, 72°C for 45 s), 72°C for 10 min, hold at 4°C]. ..

    Polymerase Chain Reaction:

    Article Title: Defective Atg16l1 in intestinal epithelial cells links to altered fecal microbiota and metabolic shifts during pregnancy in mice.
    Article Snippet: .. Each primer contained additional sequences for a 12 base Golay barcode, an Illumina adaptor, and a linker sequence.35 PCR was performed using the Phusion Hot Start Flex 2X Master Mix (NEB) in a GeneAmp PCR system 9700 (Applied Biosystems) and the following program [98°C for 3 min, 25× (98°C for 20 s, 55°C for 30 s, 72°C for 45 s), 72°C for 10 min, hold at 4°C]. ..

    Article Title: Guidestar: a spike-in approach to improve RNA detection accuracy in imaging-based spatial transcriptomics
    Article Snippet: The probe library (Twist Bioscience) was amplified following a previously published protocol . .. First, we amplified the oligonucleotide pool by limited-cycle PCR using Phusion Hot Start Flex 2X Master Mix (NEB, cat. no. M0536L), using an annealing temperature of 66°C. ..

    Article Title: A Proximity MAP of RAB GTPases
    Article Snippet: .. Human RABs were PCR amplified with the Phusion Hot Start Flex 2X master mix (M0536; New England Biolabs) from a cDNA library generated from mixed total RNA extracted with Trizol reagent (15596026: Thermo Scientific) from HeLa, HCT116, and MCF7 cells. .. PCR-amplified RABs were then cloned in-frame with MYC:APEX2 through Gibson ligation using the In-Fusion HD kit (639650; Takara Bio) in a modified pGLAP1 vector to yield the MYC:APEX2:RABs expression vector.

    Article Title: Defective Atg16l1 in intestinal epithelial cells links to altered fecal microbiota and metabolic shifts during pregnancy in mice
    Article Snippet: Each primer contained additional sequences for a 12 base Golay barcode, an Illumina adaptor, and a linker sequence. .. PCR was performed using the Phusion Hot Start Flex 2X Master Mix (NEB) in a GeneAmp PCR system 9700 (Applied Biosystems) and the following program [98°C for 3 min, 25× (98°C for 20 s, 55°C for 30 s, 72°C for 45 s), 72°C for 10 min, hold at 4°C]. ..

    Amplification:

    Article Title: Assessment of the Genetic Diversity of Atlantic Bottlenose Dolphin ( Tursiops truncatus ) Strandings in the Mississippi Sound (USA)
    Article Snippet: The quantity and purity of gDNA were assessed with a Nanodrop One spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. The complete mitogenome was amplified from extracted dolphin genomic DNA (20-30 ng) using four specific primer pairs (Table S2) with overlapping amplicon regions and Phusion Hot Start Flex Master Mix (New England Biolabs, NEB, Ipswich, MA, USA). .. After AMpure XP beads (Beckman Coulter, Indianapolis IN, USA) cleanup, the amplicon pools (four amplicons per pool per sample) were used for Nanopore barcoded amplicon library preparation with Nanopore Ligation Sequencing Kit (SQK LSK109; Oxford Nanopore Technologies, Oxford, UK) and Native Barcoding Expansion 96 Kit (EXP-NBD196; Oxford Nanopore Technologies, Oxford, UK) and sequenced on a GridION sequencer (Oxford Nanopore Technologies, Oxford, UK) using a Flongle flow cell (FLO-FLG001; Oxford Nanopore Technologies, Oxford, UK).

    Article Title: Guidestar: a spike-in approach to improve RNA detection accuracy in imaging-based spatial transcriptomics
    Article Snippet: The probe library (Twist Bioscience) was amplified following a previously published protocol . .. First, we amplified the oligonucleotide pool by limited-cycle PCR using Phusion Hot Start Flex 2X Master Mix (NEB, cat. no. M0536L), using an annealing temperature of 66°C. ..

    Article Title: Cardiac Applications of CRISPR/AAV-Mediated Precise Genome Editing
    Article Snippet: For preparation of amplicon-seq libraries from RNA, for each mouse, duplicate 2ug RNA samples were reverse transcribed using Oligo-dT with SMARTScribe Reverse Transcriptase (Takara 639537) following the manufacturer instructions. .. For each cDNA sample, 5ul of cDNA was amplified in duplicate Phusion Hot Start Flex (NEB M0536L) reactions, with a touchdown protocol for 15 cycles, decreasing 1°C per cycle from 70°C to 55°C annealing temperature. ..

    Article Title: A Proximity MAP of RAB GTPases
    Article Snippet: .. Human RABs were PCR amplified with the Phusion Hot Start Flex 2X master mix (M0536; New England Biolabs) from a cDNA library generated from mixed total RNA extracted with Trizol reagent (15596026: Thermo Scientific) from HeLa, HCT116, and MCF7 cells. .. PCR-amplified RABs were then cloned in-frame with MYC:APEX2 through Gibson ligation using the In-Fusion HD kit (639650; Takara Bio) in a modified pGLAP1 vector to yield the MYC:APEX2:RABs expression vector.

    cDNA Library Assay:

    Article Title: A Proximity MAP of RAB GTPases
    Article Snippet: .. Human RABs were PCR amplified with the Phusion Hot Start Flex 2X master mix (M0536; New England Biolabs) from a cDNA library generated from mixed total RNA extracted with Trizol reagent (15596026: Thermo Scientific) from HeLa, HCT116, and MCF7 cells. .. PCR-amplified RABs were then cloned in-frame with MYC:APEX2 through Gibson ligation using the In-Fusion HD kit (639650; Takara Bio) in a modified pGLAP1 vector to yield the MYC:APEX2:RABs expression vector.

    Generated:

    Article Title: A Proximity MAP of RAB GTPases
    Article Snippet: .. Human RABs were PCR amplified with the Phusion Hot Start Flex 2X master mix (M0536; New England Biolabs) from a cDNA library generated from mixed total RNA extracted with Trizol reagent (15596026: Thermo Scientific) from HeLa, HCT116, and MCF7 cells. .. PCR-amplified RABs were then cloned in-frame with MYC:APEX2 through Gibson ligation using the In-Fusion HD kit (639650; Takara Bio) in a modified pGLAP1 vector to yield the MYC:APEX2:RABs expression vector.



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