m0536 maxima h minus reverse transcriptase thermofisher (New England Biolabs)

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New England Biolabs m0536 maxima h minus reverse transcriptase thermofisher
M0536 Maxima H Minus Reverse Transcriptase Thermofisher, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 455 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stat+flex+software+program/Phusion+Hot+Start+Flex+Master+Mix/pm36580914-178-49-45
Average 99 stars, based on 455 article reviews

m0536 maxima h minus reverse transcriptase thermofisher - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Identification of gut microbiota features of diarrheic calves using the full-length 16S rRNA gene amplicon sequencing and machine learning
Article Snippet: The concentration and quality of the DNA were measured with the NanoDrop One (ThermoFisher Scientific Inc., MA, USA). .. The full-length 16S rRNA gene was amplified using the bacterial universal primer pairs 27F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTT-3’) [ , ] and Phusion Hot Start Flex 2X Master Mix (New England Biolabs, MA, USA). .. The resulting amplicon was purified using Agencourt AMPure XP beads (Beckman Coulter, CA, USA) and barcoded with the Native Barcoding Expansion 96 Kit (Oxford Nanopore Technologies, Oxford, UK).

Article Title: Multi-omics analysis of gut microbiota and metabolome reveals potential biomarkers in pediatric snoring
Article Snippet: DNA concentration and purity were quantified using the Qubit dsDNA HS Assay Kit (catalog No. Q32854 , Invitrogen, USA) and a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific, USA), respectively. .. PCR amplification was performed with Phusion Hot Start Flex 2X Master Mix (catalog No. M0536L, NEB, USA), and custom-synthesized primers targeting the V3-V4 hypervariable region (341 F: 5′-CCTACGGGNGGCWGCAG-3′; 805R: 5′-GACTACHVGGGTATCTAATCC-3′) were obtained from Sangon Biotech (China). .. PCR products were purified using AMPure XT Beads (catalog No. A63880, Beckman Coulter, USA), and library construction was performed using the Nextera XT Index Kit (catalog No. FC-131-1001, Illumina, USA).

Article Title: Multi-omics analysis of gut microbiota and metabolome reveals potential biomarkers in pediatric snoring
Article Snippet: After extraction, DNA concentration was quantified using a Qubit fluorometer (Invitrogen, USA), and only samples with a DNA concentration of ≥ 50 ng/μL were selected for subsequent experiments to ensure the reliability of PCR amplification. .. The V3-V4 hypervariable region of the 16 S rRNA gene was amplified in a 25 μL reaction system, which included 12.5 μL Phusion Hot Start Flex 2X Master Mix (catalog No. M0536L, NEB, USA), 2.5 μL forward primers (341 F: 5′-CCTACGGGNGGCWGCAG-3′), 2.5 μL reverse primers (805R: 5′-GACTACHVGGGTATCTAATCC-3′), 50 ng template DNA, and ddH 2 O to make up the total volume. .. The PCR program was set as follows: pre-denaturation at 98°C for 30 s; 25 cycles of denaturation at 98°C for 10 s, annealing at 54°C for 30 s, and extension at 72°C for 30 s; and a final extension at 72°C for 5 min. PCR products were purified using AMPure XT Beads (Beckman Coulter, USA) to remove primer dimers and non-specific amplification products, were then ligated with dual-index adapters using the Nextera XT Index Kit (Illumina, USA) to construct sequencing libraries.

Article Title: Organelle partitioning in the multi-budding yeast Aureobasidium pullulans
Article Snippet: .. Briefly, ∼1000 bp upstream and downstream of the desired integration site and the integration cassette (fluorescent tag + resistance cassette) were all amplified using PhusionTM Hot Start Flex 2X Master Mix (M0536L, NEB) following the manufacturer’s instructions. ..

Article Title: Multi-omics analysis of gut microbiota and metabolome reveals potential biomarkers in pediatric snoring.
Article Snippet: After extraction, DNA concentration was quantified using a Qubit fluorometer (Invitrogen, USA), and only samples with a DNA concentration of ≥50 ng/μL were selected for subsequent experiments to ensure the reliability of PCR amplification. .. The V3-V4 hypervariable region of the 16S rRNA gene was amplified in a 25 μL reaction system, which included 12.5 μL Phusion Hot Start Flex 2X Master Mix (catalog No. M0536L, NEB, USA), 2.5 μL forward primers (341F: 5′-CCTACGGGNGGCWGCAG-3′), 2.5 μL reverse primers (805R: 5′-GACTACHVGGGTATCTAATCC-3′), 50 ng AR TIC LE IN PR ES S template DNA, and ddH2O to make up the total volume. .. The PCR program was set as follows: pre-denaturation at 98°C for 30 s; 25 cycles of denaturation at 98°C for 10 s, annealing at 54°C for 30 s, and extension at 72°C for 30 s; and a final extension at 72°C for 5 min. PCR products were purified using AMPure XT Beads (Beckman Coulter, USA) to remove primer dimers and non-specific amplification products, were then ligated with dual-index adapters using the Nextera XT Index Kit (Illumina, USA) to construct sequencing libraries.

Article Title: Identification of gut microbiota features of diarrheic calves using the full-length 16S rRNA gene amplicon sequencing and machine learning.
Article Snippet: The concentration and quality of the DNA were measured with the NanoDrop One (ThermoFisher Scientific Inc., MA, USA). .. The full-length 16S rRNA gene was amplified using the bacterial universal primer pairs 27F (5’- A G A G T T T G A T C C T G G C T C A G-3’) and 1492R (5’- G G T T A C C T T G T T A C G A C T T-3’) [27, 28] and Phusion Hot Start Flex 2X Master Mix (New England Biolabs, MA, USA). .. The resulting amplicon was purified using Agencourt AMPure XP beads (Beckman Coulter, CA, USA) and barcoded with the Native Barcoding Expansion 96 Kit (Oxford Nanopore Technologies, Oxford, UK).

Article Title: Multi-omics analysis of gut microbiota and metabolome reveals potential biomarkers in pediatric snoring.
Article Snippet: DNA concentration and purity were quantified using the Qubit dsDNA HS Assay Kit (catalog No. Q32854, Invitrogen, USA) and a NanoDrop ND-1000 AR TIC LE IN PR ES S Spectrophotometer (Thermo Fisher Scientific, USA), respectively. .. PCR amplification was performed with Phusion Hot Start Flex 2X Master Mix (catalog No. M0536L, NEB, USA), and customsynthesized primers targeting the V3-V4 hypervariable region (341F: 5′-CCTACGGGNGGCWGCAG-3′; 805R: 5′- GACTACHVGGGTATCTAATCC-3′) were obtained from Sangon Biotech (China). .. PCR products were purified using AMPure XT Beads (catalog No. A63880, Beckman Coulter, USA), and library construction was performed using the Nextera XT Index Kit (catalog No. FC-131-1001, Illumina, USA).

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Article Title: Spatial Organization and Detection of Social Odors in Mouse Primary Olfactory System
Article Snippet: Glucose oxidase , Sigma , Cat# G2133.

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m0536 maxima h minus reverse transcriptase thermofisher (New England Biolabs)

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