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dapi staining  (MedChemExpress)


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    Structured Review

    MedChemExpress dapi staining
    Dapi Staining, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+with+dapi/DAPI/pm42365506-96-13-24
    Average 98 stars, based on 202 article reviews
    dapi staining - by Bioz Stars, 2026-11
    98/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Lysophospholipase PNPLA7 is a critical checkpoint of phosphatidylcholine catabolism and turnover for skeletal muscle homeostasis.
    Article Snippet: .. After fixation with 4% PFA, the cells were stained with BODIPY 493/503 (MedChemExpress, #HY-W090090) and DAPI. ..

    Article Title: BMAL1 deficiency exacerbates HDM-induced asthma by promoting JUN-mediated lipid peroxidation and airway epithelial ferroptosis.
    Article Snippet: .. Paraffin sections of lung tissue (4 μm) were incubated overnight at 4 ◦C with the following primary antibodies: COX2 (1:200, 27308-1-AP, Proteintech), JUN (1:200, YM8321, Immunoway) and stained with Alexa Fluor 594 Donkey anti-Rabbit IgG (H + L) secondary antibody (A21207, Invitrogen) at room temperature for 1 h, after which nuclei were counterstained with DAPI (MedChemExpress). ..

    Article Title: Targeting E-Selectin and ICAM1 Suppresses Endometriosis Progression by Disrupting the Inflammation–Fibrosis Axis and Reducing Local M2 Macrophage Infiltration
    Article Snippet: After that, HRP-conjugated secondary antibody and Cy5-Tyramide (MCE, Cat. No. HY-136247) were added for incubation. .. Finally, the cell nuclei were stained with DAPI (MCE, Cat. No. HY-D2868), the sections were mounted with an anti-fluorescence quenching mounting medium (MCE, Cat. No. HY-K1042), and the stained sections were observed under an Olympus BX63 automated fluorescence microscope (Olympus, Japan) with a 40 × objective lens. ..

    Incubation:

    Article Title: BMAL1 deficiency exacerbates HDM-induced asthma by promoting JUN-mediated lipid peroxidation and airway epithelial ferroptosis.
    Article Snippet: .. Paraffin sections of lung tissue (4 μm) were incubated overnight at 4 ◦C with the following primary antibodies: COX2 (1:200, 27308-1-AP, Proteintech), JUN (1:200, YM8321, Immunoway) and stained with Alexa Fluor 594 Donkey anti-Rabbit IgG (H + L) secondary antibody (A21207, Invitrogen) at room temperature for 1 h, after which nuclei were counterstained with DAPI (MedChemExpress). ..

    other:

    Article Title: Sirtuin 2 Contributes to Pancreatic Injury and Inflammation by Modulating Immediate Early Response 3–Mediated Ferroptosis in Acute Pancreatitis
    Article Snippet: Nrf2 positively regulates SLC7A11, whereas IER3 negatively regulates SLC7A11 by suppressing Nrf2 activation in AP. ( A and B ) Representative immunofluorescence images and relative fluorescence intensity of p-Nrf2 ( green ) costained with 4′,6-diamidino-2-phenylindole (DAPI; blue ) in AR42J cells treated with phosphate-buffered saline (PBS; Con), si-IER3, ML385 (HY-100523, MCE), si-IER3 + ML385, caerulein (AP), si-IER3 + AP, ML385 + AP, si-IER3 + ML385 + AP. ( C ) Dual-luciferase reporter assay of SLC7A11 promoter activity in AR42J cells treated with PBS (Con), si-IER3, ML385, si-IER3 + ML385, caerulein (AP), si-IER3 + AP, ML385 + AP, si-IER3 + ML385 + AP. ( D–F ) Representative Western blot images, relative protein and mRNA levels of SLC7A11 in AR42J cells treated with PBS (Con), si-IER3, ML385, si-IER3 + ML385, caerulein (AP), si-IER3 + AP, ML385 + AP, si-IER3 + ML385 + AP. ( G and H ) Representative immunofluorescence images and relative fluorescence intensity of p-Nrf2 ( green ) costained with DAPI ( blue ) in AR42J cells treated with PBS (Con), pCMV-HA-IER3, TBHQ (HY-100489, MCE), pCMV-HA-IER3 + TBHQ, caerulein (AP), pCMV-HA-IER3 + AP, TBHQ + AP, pCMV-HA-IER3 + TBHQ + AP. ( I ) Dual-luciferase reporter assay of SLC7A11 promoter activity in AR42J cells treated with PBS (Con), pCMV-HA-IER3, TBHQ, pCMV-HA-IER3 + TBHQ, caerulein (AP), pCMV-HA-IER3 + AP, TBHQ + AP, pCMV-HA-IER3 + TBHQ + AP. ( J–L ) Representative Western blot images, relative protein and mRNA levels of SLC7A11 in AR42J cells treated with PBS (Con), pCMV-HA-IER3, TBHQ, pCMV-HA-IER3 + TBHQ, caerulein (AP), pCMV-HA-IER3 + AP, TBHQ + AP, pCMV-HA-IER3 + TBHQ + AP.

    Article Title: Enrichment of Akkermansia muciniphila by red ginseng promotes GDF15 secretion and suppresses obesity in mice.
    Article Snippet: Background: Obesity is a growing global health burden with rising incidence.. Red ginseng (RGS), a traditional processed ginseng product, shows potential for improving metabolic parameters, though its anti-obesity mechanism remains incompletely understood.. Purpose: This study investigated the therapeutic effects of short-term RGS administration on obesity and sought to elucidate the underlying mechanism.

    Automated:

    Article Title: Targeting E-Selectin and ICAM1 Suppresses Endometriosis Progression by Disrupting the Inflammation–Fibrosis Axis and Reducing Local M2 Macrophage Infiltration
    Article Snippet: After that, HRP-conjugated secondary antibody and Cy5-Tyramide (MCE, Cat. No. HY-136247) were added for incubation. .. Finally, the cell nuclei were stained with DAPI (MCE, Cat. No. HY-D2868), the sections were mounted with an anti-fluorescence quenching mounting medium (MCE, Cat. No. HY-K1042), and the stained sections were observed under an Olympus BX63 automated fluorescence microscope (Olympus, Japan) with a 40 × objective lens. ..

    Fluorescence:

    Article Title: Targeting E-Selectin and ICAM1 Suppresses Endometriosis Progression by Disrupting the Inflammation–Fibrosis Axis and Reducing Local M2 Macrophage Infiltration
    Article Snippet: After that, HRP-conjugated secondary antibody and Cy5-Tyramide (MCE, Cat. No. HY-136247) were added for incubation. .. Finally, the cell nuclei were stained with DAPI (MCE, Cat. No. HY-D2868), the sections were mounted with an anti-fluorescence quenching mounting medium (MCE, Cat. No. HY-K1042), and the stained sections were observed under an Olympus BX63 automated fluorescence microscope (Olympus, Japan) with a 40 × objective lens. ..

    Article Title: Branched-chain amino acid depletion and adolescent myopia progression through mTORC1-dependent scleral remodeling
    Article Snippet: For immunofluorescence, cells were fixed, stained with anti-α-SMA primary 710 antibody overnight at 4 °C, followed by incubation with CoraLite488-conjugated secondary 711 Jo u n al Pr e-p roo f 24 antibody (Proteintech, Hubei, China; Cat#SA00013-2) for 1 h at room temperature in the dark. .. 712 The nuclei were counterstained with DAPI (MedChemExpress, New Jersey, USA; Cat#HY-713 D1738) for 5 min at room temperature in the dark, and fluorescence images were captured using 714 a confocal laser scanning microscope (FV4000, Olympus, Tokyo, Japan). .. Serum amino acids 715 and TGF-β were measured by ELISA (MyBioSource, California, USA; Cat#MBS2612526, 716 Cat#MBS7276965, Cat#MBS288119, Cat#MBS2701292) according to the manufacturer’s 717 instructions.

    Microscopy:

    Article Title: Targeting E-Selectin and ICAM1 Suppresses Endometriosis Progression by Disrupting the Inflammation–Fibrosis Axis and Reducing Local M2 Macrophage Infiltration
    Article Snippet: After that, HRP-conjugated secondary antibody and Cy5-Tyramide (MCE, Cat. No. HY-136247) were added for incubation. .. Finally, the cell nuclei were stained with DAPI (MCE, Cat. No. HY-D2868), the sections were mounted with an anti-fluorescence quenching mounting medium (MCE, Cat. No. HY-K1042), and the stained sections were observed under an Olympus BX63 automated fluorescence microscope (Olympus, Japan) with a 40 × objective lens. ..

    Confocal:

    Article Title: Branched-chain amino acid depletion and adolescent myopia progression through mTORC1-dependent scleral remodeling
    Article Snippet: For immunofluorescence, cells were fixed, stained with anti-α-SMA primary 710 antibody overnight at 4 °C, followed by incubation with CoraLite488-conjugated secondary 711 Jo u n al Pr e-p roo f 24 antibody (Proteintech, Hubei, China; Cat#SA00013-2) for 1 h at room temperature in the dark. .. 712 The nuclei were counterstained with DAPI (MedChemExpress, New Jersey, USA; Cat#HY-713 D1738) for 5 min at room temperature in the dark, and fluorescence images were captured using 714 a confocal laser scanning microscope (FV4000, Olympus, Tokyo, Japan). .. Serum amino acids 715 and TGF-β were measured by ELISA (MyBioSource, California, USA; Cat#MBS2612526, 716 Cat#MBS7276965, Cat#MBS288119, Cat#MBS2701292) according to the manufacturer’s 717 instructions.

    Laser-Scanning Microscopy:

    Article Title: Branched-chain amino acid depletion and adolescent myopia progression through mTORC1-dependent scleral remodeling
    Article Snippet: For immunofluorescence, cells were fixed, stained with anti-α-SMA primary 710 antibody overnight at 4 °C, followed by incubation with CoraLite488-conjugated secondary 711 Jo u n al Pr e-p roo f 24 antibody (Proteintech, Hubei, China; Cat#SA00013-2) for 1 h at room temperature in the dark. .. 712 The nuclei were counterstained with DAPI (MedChemExpress, New Jersey, USA; Cat#HY-713 D1738) for 5 min at room temperature in the dark, and fluorescence images were captured using 714 a confocal laser scanning microscope (FV4000, Olympus, Tokyo, Japan). .. Serum amino acids 715 and TGF-β were measured by ELISA (MyBioSource, California, USA; Cat#MBS2612526, 716 Cat#MBS7276965, Cat#MBS288119, Cat#MBS2701292) according to the manufacturer’s 717 instructions.



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing