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diff-quik® fixative/stain set  (Dade Behring)

 
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    Dade Behring diff-quik® fixative/stain set
    Diff Quik® Fixative/Stain Set, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+set+diff-quik/diff+quik+stain+set/pm40468407-55-19-22
    Average 90 stars, based on 1 article reviews
    diff-quik® fixative/stain set - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Suspension:

    Article Title: In vitro effects of antidepressants on human sperm function.
    Article Snippet: Chromatin/DNA integrity was assessed using a modified version of the Diff-Quik® rapid staining kit (Dade Behring Inc., Newark, NJ, USA), which is typically used to evaluate sperm morphology but can be applied to measure chromatin integrity in a straightforward and reproducible manner as described elsewhere.20 Briefly, a smear was prepared using 10 μl of the sample and allowed to air dry.

    Article Title: Beneficial effects of oral antioxidant supplementation on semen quality parameters, reproductive hormones, and sperm DNA integrity in men with idiopathic oligoasthenoteratozoospermia
    Article Snippet: The evaluation of spermatozoon morphology was based on modified David criteria and was performed using a Diff-Quik kit (Dade Behring AG) containing one stain fixative and two stains (A and B).

    Article Title: Sputum quality affects assessment of airway microbiology in childhood asthma
    Article Snippet: The remaining cell suspension was resuspended to 1 × 10 6 cells/mL and cytospin slides prepared and stained using a Diff-Quik ® fixative/stain set (Dade Behring, Deerfield, IL).

    Article Title: The Effects of Varicocelectomy on Sperm DNA Fragmentation and Conventional Semen Parameters in Men with Severe Oligoasthenoteratozoospermia: A Prospective Study
    Article Snippet: The evaluation of the spermatozoa morphology was made based on Modified David criteria and was evaluated using the Diff-Quik kit (Dade Behring AG, Switzerland) contains one stain fixative and two stains (A, B).

    Article Title: Gas6 induces AIM to suppress acute lung injury in mice by inhibiting NLRP3 inflammasome activation and inducing autophagy
    Article Snippet: In addition, BAL cytospins were stained with the Diff-Quik kit (Dade Behring, Newark, DE, USA) for differentiation of alveolar macrophages and neutrophils ( , ).

    Article Title: Evaluation of Sperm DNA Fragmentation Using Halosperm Technique after the Freezing–Thawing Process in Men: A Study on the Validation of the SCD Protocol
    Article Snippet: The evaluation of the spermatozoa morphology was made based on David’s modified classification using Diff-Quik kit (Dade Behring Diagnostics AG, Switzerland) which includes one stain fixative and two types of stain (A and B) ( ).

    Article Title: In vitro effects of antidepressants on human sperm function
    Article Snippet: Chromatin/DNA integrity was assessed using a modified version of the Diff-Quik rapid staining kit (Dade Behring Inc., Newark, NJ, USA), which is typically used to evaluate sperm morphology but can be applied to measure chromatin integrity in a straightforward and reproducible manner as described elsewhere.

    Article Title: Sputum sample quality selection in microbiota research; a chance to improve methods??
    Article Snippet: The remaining cell suspension was resuspended to 1 × 106 cells/mL and cytospin slides prepared and stained using a Diff-Quik® fixative/stain set (Dade Behring, Deerfield, IL).

    Staining:

    Article Title: In vitro effects of antidepressants on human sperm function.
    Article Snippet: Chromatin/DNA integrity was assessed using a modified version of the Diff-Quik® rapid staining kit (Dade Behring Inc., Newark, NJ, USA), which is typically used to evaluate sperm morphology but can be applied to measure chromatin integrity in a straightforward and reproducible manner as described elsewhere.20 Briefly, a smear was prepared using 10 μl of the sample and allowed to air dry.

    Article Title: Beneficial effects of oral antioxidant supplementation on semen quality parameters, reproductive hormones, and sperm DNA integrity in men with idiopathic oligoasthenoteratozoospermia
    Article Snippet: The evaluation of spermatozoon morphology was based on modified David criteria and was performed using a Diff-Quik kit (Dade Behring AG) containing one stain fixative and two stains (A and B).

    Article Title: Sputum quality affects assessment of airway microbiology in childhood asthma
    Article Snippet: The remaining cell suspension was resuspended to 1 × 10 6 cells/mL and cytospin slides prepared and stained using a Diff-Quik ® fixative/stain set (Dade Behring, Deerfield, IL).

    Article Title: The Effects of Varicocelectomy on Sperm DNA Fragmentation and Conventional Semen Parameters in Men with Severe Oligoasthenoteratozoospermia: A Prospective Study
    Article Snippet: The evaluation of the spermatozoa morphology was made based on Modified David criteria and was evaluated using the Diff-Quik kit (Dade Behring AG, Switzerland) contains one stain fixative and two stains (A, B).

    Article Title: Gas6 induces AIM to suppress acute lung injury in mice by inhibiting NLRP3 inflammasome activation and inducing autophagy
    Article Snippet: In addition, BAL cytospins were stained with the Diff-Quik kit (Dade Behring, Newark, DE, USA) for differentiation of alveolar macrophages and neutrophils ( , ).

    Article Title: Evaluation of Sperm DNA Fragmentation Using Halosperm Technique after the Freezing–Thawing Process in Men: A Study on the Validation of the SCD Protocol
    Article Snippet: The evaluation of the spermatozoa morphology was made based on David’s modified classification using Diff-Quik kit (Dade Behring Diagnostics AG, Switzerland) which includes one stain fixative and two types of stain (A and B) ( ).

    Article Title: In vitro effects of antidepressants on human sperm function
    Article Snippet: Chromatin/DNA integrity was assessed using a modified version of the Diff-Quik rapid staining kit (Dade Behring Inc., Newark, NJ, USA), which is typically used to evaluate sperm morphology but can be applied to measure chromatin integrity in a straightforward and reproducible manner as described elsewhere.

    Article Title: Sputum sample quality selection in microbiota research; a chance to improve methods??
    Article Snippet: The remaining cell suspension was resuspended to 1 × 106 cells/mL and cytospin slides prepared and stained using a Diff-Quik® fixative/stain set (Dade Behring, Deerfield, IL).

    Diff-Quik:

    Article Title: In vitro effects of antidepressants on human sperm function.
    Article Snippet: Chromatin/DNA integrity was assessed using a modified version of the Diff-Quik® rapid staining kit (Dade Behring Inc., Newark, NJ, USA), which is typically used to evaluate sperm morphology but can be applied to measure chromatin integrity in a straightforward and reproducible manner as described elsewhere.20 Briefly, a smear was prepared using 10 μl of the sample and allowed to air dry.

    Article Title: Beneficial effects of oral antioxidant supplementation on semen quality parameters, reproductive hormones, and sperm DNA integrity in men with idiopathic oligoasthenoteratozoospermia
    Article Snippet: The evaluation of spermatozoon morphology was based on modified David criteria and was performed using a Diff-Quik kit (Dade Behring AG) containing one stain fixative and two stains (A and B).

    Article Title: Sputum quality affects assessment of airway microbiology in childhood asthma
    Article Snippet: The remaining cell suspension was resuspended to 1 × 10 6 cells/mL and cytospin slides prepared and stained using a Diff-Quik ® fixative/stain set (Dade Behring, Deerfield, IL).

    Article Title: The Effects of Varicocelectomy on Sperm DNA Fragmentation and Conventional Semen Parameters in Men with Severe Oligoasthenoteratozoospermia: A Prospective Study
    Article Snippet: The evaluation of the spermatozoa morphology was made based on Modified David criteria and was evaluated using the Diff-Quik kit (Dade Behring AG, Switzerland) contains one stain fixative and two stains (A, B).

    Article Title: Gas6 induces AIM to suppress acute lung injury in mice by inhibiting NLRP3 inflammasome activation and inducing autophagy
    Article Snippet: In addition, BAL cytospins were stained with the Diff-Quik kit (Dade Behring, Newark, DE, USA) for differentiation of alveolar macrophages and neutrophils ( , ).

    Article Title: Evaluation of Sperm DNA Fragmentation Using Halosperm Technique after the Freezing–Thawing Process in Men: A Study on the Validation of the SCD Protocol
    Article Snippet: The evaluation of the spermatozoa morphology was made based on David’s modified classification using Diff-Quik kit (Dade Behring Diagnostics AG, Switzerland) which includes one stain fixative and two types of stain (A and B) ( ).

    Article Title: In vitro effects of antidepressants on human sperm function
    Article Snippet: Chromatin/DNA integrity was assessed using a modified version of the Diff-Quik rapid staining kit (Dade Behring Inc., Newark, NJ, USA), which is typically used to evaluate sperm morphology but can be applied to measure chromatin integrity in a straightforward and reproducible manner as described elsewhere.

    Article Title: Sputum sample quality selection in microbiota research; a chance to improve methods??
    Article Snippet: The remaining cell suspension was resuspended to 1 × 106 cells/mL and cytospin slides prepared and stained using a Diff-Quik® fixative/stain set (Dade Behring, Deerfield, IL).

    Modification:

    Article Title: In vitro effects of antidepressants on human sperm function.
    Article Snippet: Chromatin/DNA integrity was assessed using a modified version of the Diff-Quik® rapid staining kit (Dade Behring Inc., Newark, NJ, USA), which is typically used to evaluate sperm morphology but can be applied to measure chromatin integrity in a straightforward and reproducible manner as described elsewhere.20 Briefly, a smear was prepared using 10 μl of the sample and allowed to air dry.

    Article Title: Beneficial effects of oral antioxidant supplementation on semen quality parameters, reproductive hormones, and sperm DNA integrity in men with idiopathic oligoasthenoteratozoospermia
    Article Snippet: The evaluation of spermatozoon morphology was based on modified David criteria and was performed using a Diff-Quik kit (Dade Behring AG) containing one stain fixative and two stains (A and B).

    Article Title: Sputum quality affects assessment of airway microbiology in childhood asthma
    Article Snippet: The remaining cell suspension was resuspended to 1 × 10 6 cells/mL and cytospin slides prepared and stained using a Diff-Quik ® fixative/stain set (Dade Behring, Deerfield, IL).

    Article Title: The Effects of Varicocelectomy on Sperm DNA Fragmentation and Conventional Semen Parameters in Men with Severe Oligoasthenoteratozoospermia: A Prospective Study
    Article Snippet: The evaluation of the spermatozoa morphology was made based on Modified David criteria and was evaluated using the Diff-Quik kit (Dade Behring AG, Switzerland) contains one stain fixative and two stains (A, B).

    Article Title: Gas6 induces AIM to suppress acute lung injury in mice by inhibiting NLRP3 inflammasome activation and inducing autophagy
    Article Snippet: In addition, BAL cytospins were stained with the Diff-Quik kit (Dade Behring, Newark, DE, USA) for differentiation of alveolar macrophages and neutrophils ( , ).

    Article Title: Evaluation of Sperm DNA Fragmentation Using Halosperm Technique after the Freezing–Thawing Process in Men: A Study on the Validation of the SCD Protocol
    Article Snippet: The evaluation of the spermatozoa morphology was made based on David’s modified classification using Diff-Quik kit (Dade Behring Diagnostics AG, Switzerland) which includes one stain fixative and two types of stain (A and B) ( ).

    Article Title: In vitro effects of antidepressants on human sperm function
    Article Snippet: Chromatin/DNA integrity was assessed using a modified version of the Diff-Quik rapid staining kit (Dade Behring Inc., Newark, NJ, USA), which is typically used to evaluate sperm morphology but can be applied to measure chromatin integrity in a straightforward and reproducible manner as described elsewhere.

    Article Title: Sputum sample quality selection in microbiota research; a chance to improve methods??
    Article Snippet: The remaining cell suspension was resuspended to 1 × 106 cells/mL and cytospin slides prepared and stained using a Diff-Quik® fixative/stain set (Dade Behring, Deerfield, IL).



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    Anti-asthmatic effect of oleic acid according to BALF and serum IgE analysis and histopathological observation. The populations of (A) WBCs and (B) eosinophils (Eos) in the bronchoalveolar lavage fluid (BALF) were elevated due to OVA exposure and subsequently reduced by oleic acid treatment. Notably, oleic acid reduced the number of white blood cells and eosinophils in a concentration-dependent manner (n = 4). (C) Observation of inflammatory cells in BALF through Diff Quik staining showed a dose-dependent decrease with oleic acid treatment. Scale bar, 100 μm; magnification, 400×. (D) Serum IgE levels, which were elevated in the OVA-induced group, decreased in a dose-dependent manner with oleic acid treatment at various concentrations (n = 8). (E) Lung morphology, characterized by hypersecretion of mucus, proliferation of epithelial cells, and infiltration of inflammatory cells around the bronchoalveolar ducts and blood vessels, which were altered due to OVA, improved with oleic acid treatment in a dose-dependent manner. Scale bar, 100 μm; magnification, × 200. (F) Bronchoalveolar mucus secretion induced by OVA was reduced by treatment with varying concentrations of oleic acid (in a dose-dependent manner). Scale bar, 100 μm; magnification, × 200. a, CON; b, OVA; c, 1 mg/kg DEX; d, 50 mg/kg oleic acid; e, 125 mg/kg oleic acid; f, 250 mg/kg oleic acid. The results are expressed as the mean ± standard deviation. * p < 0.05 vs. CON; ** p < 0.001 vs. CON; $ p < 0.05 vs. OVA; $$ p < 0.001 vs. OVA; # p < 0.05 vs. 1 mg/kg DEX; ## p < 0.001 vs. 1 mg/kg DEX; @ p < 0.05 vs. 50 mg/kg oleic acid; @@ p < 0.001 vs. 50 mg/kg oleic acid; & p < 0.05 vs. 125 mg/kg oleic acid.

    Journal: Frontiers in Immunology

    Article Title: Oleic acid attenuates asthma pathogenesis via Th1/Th2 immune cell modulation, TLR3/4-NF-κB-related inflammation suppression, and intrinsic apoptotic pathway induction

    doi: 10.3389/fimmu.2024.1429591

    Figure Lengend Snippet: Anti-asthmatic effect of oleic acid according to BALF and serum IgE analysis and histopathological observation. The populations of (A) WBCs and (B) eosinophils (Eos) in the bronchoalveolar lavage fluid (BALF) were elevated due to OVA exposure and subsequently reduced by oleic acid treatment. Notably, oleic acid reduced the number of white blood cells and eosinophils in a concentration-dependent manner (n = 4). (C) Observation of inflammatory cells in BALF through Diff Quik staining showed a dose-dependent decrease with oleic acid treatment. Scale bar, 100 μm; magnification, 400×. (D) Serum IgE levels, which were elevated in the OVA-induced group, decreased in a dose-dependent manner with oleic acid treatment at various concentrations (n = 8). (E) Lung morphology, characterized by hypersecretion of mucus, proliferation of epithelial cells, and infiltration of inflammatory cells around the bronchoalveolar ducts and blood vessels, which were altered due to OVA, improved with oleic acid treatment in a dose-dependent manner. Scale bar, 100 μm; magnification, × 200. (F) Bronchoalveolar mucus secretion induced by OVA was reduced by treatment with varying concentrations of oleic acid (in a dose-dependent manner). Scale bar, 100 μm; magnification, × 200. a, CON; b, OVA; c, 1 mg/kg DEX; d, 50 mg/kg oleic acid; e, 125 mg/kg oleic acid; f, 250 mg/kg oleic acid. The results are expressed as the mean ± standard deviation. * p < 0.05 vs. CON; ** p < 0.001 vs. CON; $ p < 0.05 vs. OVA; $$ p < 0.001 vs. OVA; # p < 0.05 vs. 1 mg/kg DEX; ## p < 0.001 vs. 1 mg/kg DEX; @ p < 0.05 vs. 50 mg/kg oleic acid; @@ p < 0.001 vs. 50 mg/kg oleic acid; & p < 0.05 vs. 125 mg/kg oleic acid.

    Article Snippet: Differential cell counts were performed on the resuspended BALF white blood cells (WBCs) using a Hemavet Multispecies Hematology System (Drew Scientific, Waterbury, CT, USA) and the Diff Quik staining set (Thermo Fisher Scientific).

    Techniques: Concentration Assay, Diff-Quik, Staining, Standard Deviation