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split-gal4 components gal4dbd-3xnb12701  (NanoTag Biotechnologies GmbH)

 
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    NanoTag Biotechnologies GmbH split-gal4 components gal4dbd-3xnb12701
    Split Gal4 Components Gal4dbd 3xnb12701, supplied by NanoTag Biotechnologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/split+components/nanotag+split+gal4/pm37276389-91-23-22
    Average 90 stars, based on 1 article reviews
    split-gal4 components gal4dbd-3xnb12701 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80
    Article Snippet: To test the transcriptional activation strength of each system in cell culture, we transiently transfected either split-intein components (Gal4 N-int and Gal4 C-int ) or NanoTag Split-Gal4 components (Gal4DBD-3xNb12701 and Gal4AD-1x127D01), all driven by a constitutive Actin5c promoter, into S2R+ cells, along with a green fluorescent protein reporter (UAS:GFP).

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80.
    Article Snippet: To test the transcriptional activation strength of each system in cell culture, we transiently transfected either split-intein components (Gal4N-int and Gal4C-int) or NanoTag Split-Gal4 components (Gal4DBD-3xNb12701 and Gal4AD1x127D01), all driven by a constitutive Actin5c promoter, into esg-T2A-Gal4N-int ∩ tub-Gal4C-int esg-T2A-Gal4N-int ∩ Dl-T2A-Gal4C-int Dl-T2A-Gal4C-int ∩ tub-Gal4N-int EG FP EG FP D AP I EG FP EG FP D AP I Myo1A-T2A-Gal4DBD ∩ tub-VP16 Myo1A-T2A-Gal4N-int ∩ tub-Gal4C-int esg-T2A-Gal4N-int ∩ tub-Gal4C-int esg-T2A-Gal4DBD ∩ tub-VP16 split-intein Gal4Original split-Gal4A Gal41-20 Gal421-881 int-N (88aa) int-C (38aa) Gal41-881 in vivo trans-splicing split-intein Gal4 Gal80 binding site DBD domain dimerization domain AD-1 AD-2 intein + EG FP EG FP D AP I B Larval midgut enterocytes Larval ISCs C Adult ISCs Fig. 2.

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80
    Article Snippet: Strikingly, both split-intein Gal4 and NanoTag split-Gal4 exhibited strong repression by Gal80, albeit slightly weaker than that observed for wild-type Gal4 ( SI Appendix , Fig. S1 ).


    Article Title: split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80.
    Article Snippet: We tested whether split-intein Gal4 and NanoTag Gal4 were repressible by Gal80 in S2R+ cells by cotransfecting these components with pTub:Gal80.

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80.
    Article Snippet: We refer to this combination as NanoTag split-Gal4.

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80.
    Article Snippet: Testing Split-Intein Gal4 and NanoTag Split-Gal4 in S2R+ Cells.

    Transfection:

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80
    Article Snippet: Pilot characterization of split-intein Gal4 and Nanotag split-Gal4 in S2R+ cells. .. Plasmids encoding Gal4, split-Gal4, split-intein Gal4, or Nanotag split-Gal4, each driven by a constitutively expressed Actin promoter, were transiently transfected into S2R+ cells, either with or without co-transfection of the Gal80 repressor. .. Pilot characterization of split-intein Gal4 and Nanotag split-Gal4 in S2R+ cells.



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    Image Search Results


    (A) The Gal4 transcription factor binds to UAS sequences to drive transcription, and can be repressed by the binding of Gal80. Gal4 is drawn here as a monomer, but functions as a dimer in vivo. (B) In the original split-Gal4 system, the Gal4DBD and a strong transcriptional activator (VP16 or p65) are each driven by separate enhancers, and reconstituted in cells by leucine zipper domains. Gal80 cannot bind or repress the split-Gal4 complex. (C) In the split-intein Gal4 system, two fragments of the Gal4 protein, each flanked by a split-intein, are independently driven by separate enhancers, and seamlessly trans-spliced to reconstitute a functional, wildtype Gal4 protein, which can be repressed by Gal80.

    Journal: bioRxiv

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80

    doi: 10.1101/2023.03.24.534001

    Figure Lengend Snippet: (A) The Gal4 transcription factor binds to UAS sequences to drive transcription, and can be repressed by the binding of Gal80. Gal4 is drawn here as a monomer, but functions as a dimer in vivo. (B) In the original split-Gal4 system, the Gal4DBD and a strong transcriptional activator (VP16 or p65) are each driven by separate enhancers, and reconstituted in cells by leucine zipper domains. Gal80 cannot bind or repress the split-Gal4 complex. (C) In the split-intein Gal4 system, two fragments of the Gal4 protein, each flanked by a split-intein, are independently driven by separate enhancers, and seamlessly trans-spliced to reconstitute a functional, wildtype Gal4 protein, which can be repressed by Gal80.

    Article Snippet: The inserts for the original split-Gal4 components (pAW-Zip − -Gal4DBD and pAW-p65-Zip + ) were amplified from pBPZpGAL4DBDUw (Addgene 26233) and pBPp65ADZpUw (Addgene 26234), respectively. pCaSpeR-tub-Gal80 was used to test for Gal80 sensitivity in S2R+ cells.

    Techniques: Binding Assay, In Vivo, Functional Assay

    (A) Cartoon schematic of the split-intein GeneSwitch system, not drawn to scale. The same N-terminal fragment of Gal4 used in split-intein Gal4 can be combined with the C-terminus of the GeneSwitch system, which includes amino acids 21-93 of Gal4, a progesterone ligand binding domain (PR-LBD) and the p65 transcriptional activator. (B) Drug-inducible ISC tumor model using the esg -Gal4 N-int ∩ tub- GeneSwitch C-int > UAS: yki S3A . Anterior is up. Scale bar = 50 µm.

    Journal: bioRxiv

    Article Title: split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80

    doi: 10.1101/2023.03.24.534001

    Figure Lengend Snippet: (A) Cartoon schematic of the split-intein GeneSwitch system, not drawn to scale. The same N-terminal fragment of Gal4 used in split-intein Gal4 can be combined with the C-terminus of the GeneSwitch system, which includes amino acids 21-93 of Gal4, a progesterone ligand binding domain (PR-LBD) and the p65 transcriptional activator. (B) Drug-inducible ISC tumor model using the esg -Gal4 N-int ∩ tub- GeneSwitch C-int > UAS: yki S3A . Anterior is up. Scale bar = 50 µm.

    Article Snippet: The inserts for the original split-Gal4 components (pAW-Zip − -Gal4DBD and pAW-p65-Zip + ) were amplified from pBPZpGAL4DBDUw (Addgene 26233) and pBPp65ADZpUw (Addgene 26234), respectively. pCaSpeR-tub-Gal80 was used to test for Gal80 sensitivity in S2R+ cells.

    Techniques: Ligand Binding Assay