saci sphi (New England Biolabs)

New England Biolabs is a verified supplier

New England Biolabs manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

New England Biolabs saci sphi
Saci Sphi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1099 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sphi/SacI/pm41805723-243-54-55
Average 96 stars, based on 1099 article reviews

saci sphi - by Bioz Stars, 2026-10

96/100 stars

Images

other:

Article Title: The Helicobacter pylori TlpD cytoplasmic chemoreceptor requires an intact C-terminus for polar localization and function.
Article Snippet: Quantification of bands was carried out using Fiji ImageJ software for normalization of peaks to the area of the full-length TlpD peak.

Article Title: Optimized expression and antimicrobial activity of rainbow trout defensin 3 in Komagataella phaffii.
Article Snippet: The DNA gel extraction kit, yeast RNA extraction kit, and DNA marker A (25-500 bp) were obtained from Sangon Biotech Co., Ltd. (Shanghai, China).

Article Title: Seipin-defined ER sites undergo distinct enrichment of factors for the biogenesis of sterol ester rich lipid droplets
Article Snippet: SacI-HF , New England Biolabs , Cat# R3156S.

Expressing:

Article Title: Neuronally sensed oxygen drives behavior and development in human-infective, skin-penetrating nematodes
Article Snippet: The strGCaMP7f:: P2A:: strmScarlet-I cassette was originally synthesized by GenScript (Piscataway, NJ, USA) and used to generate pRP08. .. To drive expression in Sst- URX, we used SacI-HF (New England Biolabs, R3156) and AgeI-HF (New England Biolabs, R3552) to clone the Sst-gcy-36 promoter from pBMW15 into pRP08, resulting in pBMW31 ( Sst-gcy-36p::strGCaMP7f:: P2A ::strmScarlet-I::Sst-era-1 3′ UTR). .. To drive expression in Sst- BAG, we used AgeI-HF (New England Biolabs, R3552) and AvrII (New England Biolabs, R0174) to clone the strGCaMP7f:: P2A ::strmScarlet-I cassette from pBMW31 into pNB14, thus creating pBMW66 ( Sst-gcy-9p::strGCaMP7f:: P2A ::strmScarlet-I::Sst-era-1 3′ UTR).

Clone Assay:

Article Title: Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking.
Article Snippet: Scarless GFP and Gal4 HDR donor plasmids with a GMReya(shRNA) marker pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA) (Addgene #240228, DGRC #1684) and pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA) (Addgene #240229, DGRC #1685) were cloned by first mutating the EcoRI site in pBS-GMR-eya(shRNA) (Addgene #157991)68 using site directed mutagenesis (NEB, E0554S) to produce pBS-GMReya(shRNA)_EcoRImut (Addgene #240222, DGRC #1678). sfGFP-ScarlessDsRed was PCR amplified from pHD-sfGFP-ScarlessDsRed (Addgene # 80811)67, 99, and T2AGal4-ScarlessDsRed was PCR amplified from Bosch CRISPaint plasmid pCRISPaintT2A-GAL4-Hsp70100. .. Each fragment was cloned into pBS-GMReya(shRNA)_EcoRImut, which had been digested SacI and HindIII (NEB, R3156S, R3104S), by Gibson assembly. .. pEntr_CG6867_nostop (Addgene #240238, DGRC #1694) was generated by Gibson assembly of PCR-amplified coding sequence into pEntr backbone sequence.

Virus:

Article Title: Biomimetic recombinant collagen with multicopy dual-targeted hemostatic motifs for enhanced hemostasis in surgical trauma.
Article Snippet: Zikun Zhang (张梓坤), Xiaoxuan Ma (马晓轩), Zhuo Zhang (张卓), Weigang Yuwen (宇文伟刚), Zhiguang Duan (段志广), Rongzhan Fu (傅容湛), Yang Li (李阳), Chenhui Zhu (朱晨辉), Daidi Fan (范代娣) liyang2023@nwu.edu.cn (Y. Li), zch2005@nwu.edu.cn (C. Zhu) fandaidi@nwu.edu.cn (D. Fan).. Trends in Biotechnology, Month 2026, Vol. xx, No. xx https://doi.org/10.1016/j.tibtech.2026.01.017 TIBTEC 2920 No. of Pages 26 Trends in Biotechnology

CCK-8 Assay:

Lactate Dehydrogenase Assay:

Enzyme-linked Immunosorbent Assay:

Recombinant:

Software:

In Silico:

Article Title: A Genetic Method for Distinguishing Cryptic Pocillopora Species in French Polynesia without Sequencing
Article Snippet: .. A total of eight candidate enzymes were identified for further testing in silico : AciI (New England Biolabs (NEB) #R0551S, MA, USA), BanI (NEB #R0118S), BceAI (NEB #R0623S), BseYI (NEB #R0635S), EcoRV-HF (NEB #R3195S), HgaI (NEB #R0154S), NlaIV (NEB #R0126S), and SacI-HF (NEB #R3156S), and XhoI (NEB Cat # R0146S). ..

Plasmid Preparation:

Article Title: A tumor-intrinsic WNT-inhibitory NOTUM program drives immune resistance in microsatellite stable colorectal cancer.
Article Snippet: .. 1 μg 5′ homology arm was digested along with the Plasmid #129094 (Addgene with 1 μL enzymes NdeI (NEB), 1 μL enzyme SacI-HF (NEB), 5 μL of custmart buffer (NEB) topped up with water to 50 μL total reaction. ..

Review