s080983 2 vectashield vibrance antifade mounting medium vector laboratories (Vector Laboratories)

Vector Laboratories is a verified supplier

Vector Laboratories manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Vector Laboratories s080983 2 vectashield vibrance antifade mounting medium vector laboratories
S080983 2 Vectashield Vibrance Antifade Mounting Medium Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 900 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spatial+transcriptomics+st+sequencing/VECTASHIELD+Vibrance+Antifade+Mounting+Medium/pm41928518-271-14-20
Average 99 stars, based on 900 article reviews

s080983 2 vectashield vibrance antifade mounting medium vector laboratories - by Bioz Stars, 2026-11

99/100 stars

Images

Staining:

Article Title: Anterior cingulate feedback facilitates rapid global context modulation in primary visual cortex.
Article Snippet: .. Sections were stained with DAPI (ThermoFisher, D1306, 1μg/ml) and mounted using antifade medium (VectorLabs, H-1700–2). .. 2-Photon image processing and analysis Calcium imaging datasets were scored similarly to previous reports.15 Raw images were processed to correct motion artifacts using the ‘‘Moco’’ plugin for ImageJ (NIH).55 Regions of interest (ROIs) were selected semi-automatically from time-averaged images using an in-house written MATLAB (MathWorks) routine or with Suite 2P.56 Individual fluorescence traces were visually inspected and kept if they resembled soma (>5μm in diameter) and showed at least one calcium transient in both runs (control and oddball), and no evidence of dramatic drift.

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: Inhibition of CDK1 attenuates cardiac remodeling via suppression of fibroblast-derived TGF-β1.
Article Snippet: Activation of cyclin-dependent kinase 1 (CDK1) is implicated in fibrosis in liver and skin.. However, the role of CDK1 in cardiac remodeling remains unclear.. We investigated whether CDK1 inhibition suppresses cardiac remodeling and explored the underlying mechanisms.

Article Title: Volumetric imaging of Young's modulus and nonlinear elasticity on the micro-scale.
Article Snippet: Lipid droplets were labeled using BODIPY 493/503 (Thermo Fisher Scientific, D3922, 1:500 dilution), and nuclei counterstained with DAPI (Thermo Fisher Scientific, D1306). .. Stained sections were mounted with VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories, H-1700) and sealed with coverslips. .. Fluorescence imaging was performed using a Zeiss Axioscan 7 slide scanner (Carl Zeiss, Germany) equipped with appropriate filter sets for DAPI, FITC, and Alexa 594 channels.

Microscopy:

Article Title: Mitochondrial pyruvate carrier inhibition restrains germinal center T follicular helper cells attenuating chronic graft-versus-host disease.
Article Snippet: 199 words 44 45 Jo urn al Pr e-p roo f Abbreviations 46 2-deoxy-D-glucose (2-DG); Allogeneic hematopoietic stem cell transplantation (allo47 HSCT); Antibody-secreting cells (ASC); Bronchiolitis obliterans (BO); Bronchiolitis 48 obliterans syndrome (BOS); Chronic graft-versus-host disease (cGVHD); Extracellular 49 acidification rate (ECAR); Germinal center B-cells (GCB); Glucose transporter 1 (GLUT1); 50 Hematoxylin & Eosin (H&E) Hypoxia-inducible factors (HIFs); Intraperitoneally (i.p.); 51 Lymph nodes (LN); Mitochondrial pyruvate carrier (MPC); Overall response rate (ORR); 52 Oxidative phosphorylation (OXPHOS); Oxygen consumption rate (OCR); Peanut 53 agglutinin (PNA); Plasma cells (PC); Tricarboxylic acid (TCA); Wildtype (WT).. 54 55

Article Title: Development of an in vitro chemical hazard screening method using human iPSCs with and without metabolic activation and application to chlorinated paraffins.
Article Snippet: There is a growing need for new approach methodologies (NAMs) to screen the hazard of the increasing number of untested chemicals.. Human induced pluripotent stem cells (h-iPSCs) represent a promising resource for developing such NAMs, provided their low metabolic capacity is addressed.. Here, we developed a proof-ofconcept in vitro NAM combining h-iPSCs with pooled human liver S9 fractions (h-iPSCs-S9) to enable chemical hazard screening both with and without metabolic activation.

Reverse Transcription:

Sensitivity:

Analysis:

Cell Viability Assay:

Library Quantification:

Next-Generation Sequencing:

Isolation:

Qubit:

RNA Sequencing:

Sequencing:

Imaging:

Article Title: Biallelic loss-of-function PTPN6 variants lead to inflammatory lung disease and hemolytic anemia.
Article Snippet: After additional washes, sections were stained with DAPI nuclear stain at 1:1,000 (D1306; Invitrogen, Lot 2942291, 5 mg/ml stock concentration) for 5 min at room temperature. .. After additional washes, sections were mounted in Vectashield (cat no. H-1700; Vector Laboratories) for standard fluorescent imaging. ..

Review