h 2k b tsa58 mdx cells (Thermo Fisher)

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Thermo Fisher h 2k b tsa58 mdx cells

Efficient nuclear uptake of liposome-complexed fluorescein-labeled AO by <t>H-2Kb-tsA58</t> mdx cells. Cultured H-2Kb-tsA58 mdx myotubes were assessed for uptake of fluorescence after exposure to complexes of Lipofectin and AO 5′SS-FITC (2:1 ratio). Nuclear fluorescence was observed in ≈100% of the myotubes 3 h after transfection, with some pinpoint foci of fluorescence located in the cytoplasm and at the cell surface (B). Many of the transfected myotubes displayed multiple fluorescent nuclei (A).

H 2k B Tsa58 Mdx Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spatial+transcriptomics+data+spatial+transcriptomics+2k/Trypsin/pmc00014541-87-0-24
Average 99 stars, based on 1 article reviews

h 2k b tsa58 mdx cells - by Bioz Stars, 2026-09

99/100 stars

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1) Product Images from "Antisense-induced exon skipping and synthesis of dystrophin in the mdx mouse"

Article Title: Antisense-induced exon skipping and synthesis of dystrophin in the mdx mouse

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Efficient nuclear uptake of liposome-complexed fluorescein-labeled AO by H-2Kb-tsA58 mdx cells. Cultured H-2Kb-tsA58 mdx myotubes were assessed for uptake of fluorescence after exposure to complexes of Lipofectin and AO 5′SS-FITC (2:1 ratio). Nuclear fluorescence was observed in ≈100% of the myotubes 3 h after transfection, with some pinpoint foci of fluorescence located in the cytoplasm and at the cell surface (B). Many of the transfected myotubes displayed multiple fluorescent nuclei (A).

Figure Legend Snippet: Efficient nuclear uptake of liposome-complexed fluorescein-labeled AO by H-2Kb-tsA58 mdx cells. Cultured H-2Kb-tsA58 mdx myotubes were assessed for uptake of fluorescence after exposure to complexes of Lipofectin and AO 5′SS-FITC (2:1 ratio). Nuclear fluorescence was observed in ≈100% of the myotubes 3 h after transfection, with some pinpoint foci of fluorescence located in the cytoplasm and at the cell surface (B). Many of the transfected myotubes displayed multiple fluorescent nuclei (A).

Techniques Used: Labeling, Cell Culture, Fluorescence, Transfection

AO-induced exon skipping in H-2Kb-tsA58 mdx myoblasts. Total RNA was extracted from treated and untreated H-2Kb-tsA58 mdx cells and amplified by nested RT-PCR using primers annealing to exons 20 and 26. Transfections were carried out as described in the text with the indicated AOs. The 901-bp full-length transcript was detected in all samples except the PCR negative (-ve) control. Lane M, size markers. A shorter product of 688 bp (arrow), corresponding to the removal of exon 23, was amplified from cell extracts transfected with AO 5′SS-FITC and AO 5′SS-25.

Figure Legend Snippet: AO-induced exon skipping in H-2Kb-tsA58 mdx myoblasts. Total RNA was extracted from treated and untreated H-2Kb-tsA58 mdx cells and amplified by nested RT-PCR using primers annealing to exons 20 and 26. Transfections were carried out as described in the text with the indicated AOs. The 901-bp full-length transcript was detected in all samples except the PCR negative (-ve) control. Lane M, size markers. A shorter product of 688 bp (arrow), corresponding to the removal of exon 23, was amplified from cell extracts transfected with AO 5′SS-FITC and AO 5′SS-25.

Techniques Used: Amplification, Reverse Transcription Polymerase Chain Reaction, Transfection

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