other:
Cloning:Article Title: Myxobacterium-Produced Antibiotic TA (Myxovirescin) Inhibits Type II Signal Peptidase
Article Snippet: .. MG1655 Wild type ATCC DW37 imp4213 Kanr BAS849 P1:CAG18620¡carB3092::Tn10(kan) imp4213 This study E609L lpp::Tn10 Tetr K. Miller; 19 YX23 imp4213 lpp::IS4 Kanr DW37 NTG mutagenesis¡select TAr This study YX104 pCA24N-lspA Cmr MG1655 pCA24N-lspA¡select Cmr This study YX127 lpp::Tn10 pJY811 Tetr Cmr E609L pJY811¡select Cmr This study YX130 lpp::Tn10 pJY851 Tetr Cmr E609L pJY851¡select Cmr This study YX135 imp4213 pJY811 Kanr Cmr YX23 pJY811¡select Cmr This study XL1-Blue Cloning strain Tetr Stratagene ASKA library AG1 host Cmr 12 CGSC8171 F , purR106::Tn10 Tetr CGSCa CGSC6920 F , zdh-299::Tn10 Tetr CGSC CGSC7385 F , zdi-925::Tn10 Tetr CGSC CGSC6756 Hfr (PO118) zdi-57::Tn10 Tetr CGSC CGSC6728 Hfr (PO2A) pfkB20::Tn10 Tetr CGSC CGSC7387 F , zdj-276::Tn10 Tetr CGSC CGSC7390 F , zea-3068::Tn10 Tetr CGSC CGSC7234 F , ruvA60 (polar)::Tn10 Tetr CGSC CGSC6758 Hfr (PO45) zed-977::Tn10 Tetr CGSC CGSC7395 F , zed-3069::Tn10 Tetr CGSC CGSC7397 F , zef-3129::Tn10 Tetr CGSC Plasmids pJY811 pLspA-SK (WT), PBAD promoter Cm r 49 pJY851 pLspA-SR, PBAD promoter Cm r 49 pCA24N-lspA pLspA , PT5-lac promoter Cm r 12 a CGSC, Coli Genetic Stock Center. ..
Plasmid Preparation:Article Title: Identification of Genes Essential for Antibiotic-Induced Up-Regulation of Plasmid-Transfer-Genes in Cephalosporin Resistant Escherichia coli .
Article Snippet: .. Strains Genotype References MG1655/pTF2 E. coli MG1655 + blaCTX−M−1 containing IncI1 plasmid pTF2 (AmpR) Kjeldsen et al., 2015 J53-2 E. coli, RifR Appelbaum et al., 1972 ATCC R© 25922 E. coli Reference strain Ceri et al., 1999 GL100 MG1655/pTF2 1traF:lacZ, CTXR This work GL101 MG1655 rfaH:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL102 MG1655 yhiN:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL103 MG1655 waaP:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL104 MG1655 waaQ:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL105 MG1655 gnd:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL106 MG1655 pgl:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL107 MG1655/pTF2 1traF:lacZ ISEcp1:Tn5, CTXR, TmpR This work GL111 MG1655 1rfaH/pTF2, CTXR This work GL112 MG1655 1yhiN/pTF2, CTXR This work GL113 MG1655 1waaP/pTF2, CTXR This work GL114 MG1655 1waaQ/pTF2, CTXR This work GL115 MG1655 1gnd/pTF2, CTXR This work GL116 MG1655 1pgl/pTF2, CTXR This work GL117 MG1655/pTF2 1ISEcp1, CTXR This work Plasmids pKD46 reppSC101ts GemR ParaBADγ β exo Doublet et al., 2008 pKD4 repR6K γAmpR FRT KamR FRT Datsenko and Wanner, 2000 pCP20 reppSC101ts AmpR CapR cI857 λPR Cherepanov and Wackernagel, 1995 pCE36 repR6K γ KamR FRT lacZY this Ellermeier et al., 2002 Frontiers in Microbiology | www.frontiersin.org 2 September 2019 | Volume 10 | Article 2203 kanamycin resistant cassette was removed using plasmid pCP20, and the lacZ transcriptional fusion plasmid pCE36 was integrated into the tyrosine DNA recombinase (FLP) recombination target sequence at the deleted traF locus (Datsenko and Wanner, 2000; Ellermeier et al., 2002). ..
Sequencing:Article Title: Identification of Genes Essential for Antibiotic-Induced Up-Regulation of Plasmid-Transfer-Genes in Cephalosporin Resistant Escherichia coli .
Article Snippet: .. Strains Genotype References MG1655/pTF2 E. coli MG1655 + blaCTX−M−1 containing IncI1 plasmid pTF2 (AmpR) Kjeldsen et al., 2015 J53-2 E. coli, RifR Appelbaum et al., 1972 ATCC R© 25922 E. coli Reference strain Ceri et al., 1999 GL100 MG1655/pTF2 1traF:lacZ, CTXR This work GL101 MG1655 rfaH:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL102 MG1655 yhiN:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL103 MG1655 waaP:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL104 MG1655 waaQ:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL105 MG1655 gnd:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL106 MG1655 pgl:Tn5/pTF2 1traF:lacZ, CTXR, TmpR This work GL107 MG1655/pTF2 1traF:lacZ ISEcp1:Tn5, CTXR, TmpR This work GL111 MG1655 1rfaH/pTF2, CTXR This work GL112 MG1655 1yhiN/pTF2, CTXR This work GL113 MG1655 1waaP/pTF2, CTXR This work GL114 MG1655 1waaQ/pTF2, CTXR This work GL115 MG1655 1gnd/pTF2, CTXR This work GL116 MG1655 1pgl/pTF2, CTXR This work GL117 MG1655/pTF2 1ISEcp1, CTXR This work Plasmids pKD46 reppSC101ts GemR ParaBADγ β exo Doublet et al., 2008 pKD4 repR6K γAmpR FRT KamR FRT Datsenko and Wanner, 2000 pCP20 reppSC101ts AmpR CapR cI857 λPR Cherepanov and Wackernagel, 1995 pCE36 repR6K γ KamR FRT lacZY this Ellermeier et al., 2002 Frontiers in Microbiology | www.frontiersin.org 2 September 2019 | Volume 10 | Article 2203 kanamycin resistant cassette was removed using plasmid pCP20, and the lacZ transcriptional fusion plasmid pCE36 was integrated into the tyrosine DNA recombinase (FLP) recombination target sequence at the deleted traF locus (Datsenko and Wanner, 2000; Ellermeier et al., 2002). ..
Cell Culture:Article Title: Indolinones compounds and their use in the treatment of fibrotic diseases
Article Snippet: .. VEGF165/FGF-Basic Induced Endothelial Cell Proliferation TeloHAEC (telomerase immortalized Human Aortic Endothelial Cells; ATCC) are seeded in to 96 well tissue culture plates at a cell density of 4000 cells per well (100 μl) in endothelial cell starvation medium (0.5% FBS, without FGF and VEGF growth factors) and cultured for 3 hr (37 C/5% CO2/95% O2). .. Cells are pre-incubated with test compound for 1 hr followed by stimulation with rhuVEGF165 (10 ng/ml, R&D Systems) or rhuFGF-basic (5 ng/ml, R&D Systems) for 48 hr.
Article Title: Indole derivatives and their use as protein kinase inhibitors
Article Snippet: .. VEGF165/FGF-Basic Induced Endothelial Cell Proliferation TeloHAEC (telomerase immortalized Human Aortic Endothelial Cells; ATCC) are seeded in to 96 well tissue culture plates at a cell density of 4000 cells per well (100 μl) in endothelial cell starvation medium (0.5% FBS, without FGF and VEGF growth factors) and cultured for 3 hr (37 C/5% CO2/95% O2). .. Cells are pre-incubated with test compound for 1 hr followed by stimulation with rhuVEGF165 (10 ng/ml, R&D Systems) or rhuFGF-basic (5 ng/ml, R&D Systems) for 48 hr.
Cytotoxicity Assay:Article Title: Dynamic Corrosion and Material Characteristics of Mg–Zn–Zr Mini‐Tubes: The Influence of Microstructures and Extrusion Parameters
Article Snippet: In this study, magnesium–zinc–zirconium (Mg–Zn–Zr) alloy mini-tubes that fit the diameter of cardiovascular stents are successfully fabricated using an isothermal extrusion method.. The influence of extrusion temperature and ram speed on the microstructure are examined.. In addition, this research develops a novel dynamic-corrosion apparatus for Mg alloy mini-tube examination, and supplemented with electrochemical, and biocompatibility tests, the optimal criteria for mini-tube extrusion are defined.
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