annexin v fitc cell apoptosis detection kit (Beyotime)
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Beyotime
annexin v fitc cell apoptosis detection kit
Annexin V Fitc Cell Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 916 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spatial+transcriptomics+cell-type+deconvolution/Annexin+V-mCherry+Apoptosis+Detection+Kit/pmc07952250-64-32-38
Average 96 stars, based on 916 article reviews
Annexin V Fitc Cell Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 916 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spatial+transcriptomics+cell-type+deconvolution/Annexin+V-mCherry+Apoptosis+Detection+Kit/pmc07952250-64-32-38
Average 96 stars, based on 916 article reviews
annexin v fitc cell apoptosis detection kit - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "circNRIP1 facilitates keloid progression via FXR1-mediated upregulation of miR-503-3p and miR-503-5p"
Article Title: circNRIP1 facilitates keloid progression via FXR1-mediated upregulation of miR-503-3p and miR-503-5p
Journal: International Journal of Molecular Medicine
doi: 10.3892/ijmm.2021.4903
Figure Legend Snippet: circNRIP1 is upregulated in keloid tissue and is associated with keloid progression. RT-qPCR assay was performed to detect the abundance of circNRIP1 in (A) keloid and adjacent normal skin tissue and (B) keloid-derived and normal human fibroblasts. (C) Knockdown efficiency of circNRIP1 was evaluated in keloid-derived fibroblasts by RT-qPCR. (D) Cell Counting Kit-8 assay was used to determine the viability of keloid-derived fibroblasts in the presence or absence circNRIP1 knockdown. Colony-forming ability of keloid-derived fibroblasts was (E) assessed and (F) quantified. Flow cytometric assays were conducted to evaluate the effect of circNRIP1 on (G and H) apoptosis and (I and J) cell cycle progression of keloid-derived fibroblasts. (K and L) Changes in the expression of extracellular matrix-associated regulatory proteins in keloid-derived fibroblasts were determined by western blot assay. ** P<0.01 vs. Ctrl or human normal fibroblasts. circNRIP1, circular nuclear receptor interacting protein 1; RT-q, reverse transcription-quantitative; Ctrl, control; si, small interfering; OD, optical density.
Techniques Used: Quantitative RT-PCR, Derivative Assay, Knockdown, Cell Counting, Expressing, Western Blot, Reverse Transcription, Control
Figure Legend Snippet: FXR1 promotes keloid-derived fibroblast proliferation and accumulation of extracellular matrix but inhibits apoptosis. (A and B) Knockdown efficiency of FXR1 was investigated in keloid-derived fibroblasts via western blot analysis. (C) Viability of keloid-derived fibroblasts in the presence or absence of FXR1 knockdown was detected by Cell Counting Kit-8 assay. (D and E) Proliferation of keloid-derived fibroblasts was determined by colony formation assay. (F and G) Cell apoptosis and (H and I) cell cycle analysis were determined via flow cytometric assays in siFXR1-transfected keloid-derived fibroblasts. (J and K) Expression levels of extracellular matrix-associated proteins were determined in siFXR1-transfected keloid-derived fibroblasts by western blot assay. ** P<0.01 vs. Ctrl. FXR1, Fbxo4-mediated FMR1 autosomal homolog 1; si, small interfering; Ctrl, control; OD, optical density; SMA, smooth muscle actin.
Techniques Used: Derivative Assay, Knockdown, Western Blot, Cell Counting, Colony Assay, Cell Cycle Assay, Transfection, Expressing, Control
Figure Legend Snippet: Mature miR-503 induces keloid-derived fibroblast proliferation and accumulation of extracellular matrix but inhibits apoptosis. (A) Knockdown efficiency of the miR-503-3p and miR-503-5p specific inhibitors was investigated in keloid-derived fibroblasts via reverse transcription-quantitative PCR. Viability of keloid-derived fibroblasts transfected with (B) miR-503-3p or (C) miR-503-5p inhibitor was determined by Cell Counting Kit-8 assay. (D and E) Proliferation of miR-503-3p or miR-503-5p inhibitor-transfected keloid-derived fibroblasts was measured by colony formation assay. (F and G) Apoptosis and (H) cell cycle progression in keloid-derived fibroblasts transfected with (I) miR-503-3p or (J) miR-503-5p inhibitor was assessed by flow cytometry. Western blot assays were used to determine the expression levels of extracellular matrix-associated proteins in keloid-derived fibroblasts transfected with (K and L) miR-503-3p or (M and N) miR-503-5p inhibitor. ** P<0.01 vs. Ctrl. miR, microRNA; Ctrl, control; OD, optical density; SMA, smooth muscle actin.
Techniques Used: Derivative Assay, Knockdown, Reverse Transcription, Real-time Polymerase Chain Reaction, Transfection, Cell Counting, Colony Assay, Flow Cytometry, Western Blot, Expressing, Control
Figure Legend Snippet: Schematic representation of the mechanism by which circNRIP1 promotes keloid progression. circNRIP1 induced pre-miR-503 maturation via protecting FXR1 from Fbxo4-mediated ubiquitination and degradation, which facilitated proliferation and extracellular matrix accumulation, but inhibited apoptosis, in keloid-derived fibroblasts. circNRIP1, circular nuclear receptor interacting protein 1; miR, microRNA; FXR1, Fbxo4-mediated FMR1 autosomal homolog 1; AGO2, argonaute RISC catalytic component 2.
Techniques Used: Ubiquitin Proteomics, Derivative Assay
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