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annexin v fitc pi apoptosis detection kit  (Beyotime)


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    Structured Review

    Beyotime annexin v fitc pi apoptosis detection kit
    Effect of MTX on the biological function of HUT-78 cells. (A) Chemical formula of MTX. (B) Effect of different concentrations of MTX on cell survival. (C) Flow cytometric analysis of (D) the effects of different concentrations of MTX on cells effects of <t>apoptosis.</t> Data are presented as the mean ± SD, n=3, and were analysed using a one-way ANOVA with Tukey's test. *P<0.05, **P<0.01, ***P<0.005 vs. NC. MTX, methotrexate; NC, negative control.
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 3712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spatial+transcriptomics+cell-type+deconvolution/Annexin+V-EGFP+Apoptosis+Detection+Kit/pmc07551555-57-5-11
    Average 99 stars, based on 3712 article reviews
    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "HSH2D contributes to methotrexate resistance in human T-cell acute lymphoblastic leukaemia"

    Article Title: HSH2D contributes to methotrexate resistance in human T-cell acute lymphoblastic leukaemia

    Journal: Oncology Reports

    doi: 10.3892/or.2020.7772

    Effect of MTX on the biological function of HUT-78 cells. (A) Chemical formula of MTX. (B) Effect of different concentrations of MTX on cell survival. (C) Flow cytometric analysis of (D) the effects of different concentrations of MTX on cells effects of apoptosis. Data are presented as the mean ± SD, n=3, and were analysed using a one-way ANOVA with Tukey's test. *P<0.05, **P<0.01, ***P<0.005 vs. NC. MTX, methotrexate; NC, negative control.
    Figure Legend Snippet: Effect of MTX on the biological function of HUT-78 cells. (A) Chemical formula of MTX. (B) Effect of different concentrations of MTX on cell survival. (C) Flow cytometric analysis of (D) the effects of different concentrations of MTX on cells effects of apoptosis. Data are presented as the mean ± SD, n=3, and were analysed using a one-way ANOVA with Tukey's test. *P<0.05, **P<0.01, ***P<0.005 vs. NC. MTX, methotrexate; NC, negative control.

    Techniques Used: Negative Control

    HSH2D is essential for MTX resistance of HuT-78 cells. (A) Silencing efficacy was evaluated using reverse transcription-quantitative PCR after transfection of two siRNAs and a NC of HSH2D. (B) Knockdown of HSH2D promoted apoptosis induced by MTX treatment in both MTX-resistant cell lines. (C) IC 50 value of MTX was detected for both sensitive and resistant cells using a Cell Counting Kit-8 assay. (D) EdU results of the cell proliferation of drug resistant strains with HSH2D knockdown. (E) Flow cytometry was used to detected (F) the cell cycle in drug resistant strains with HSH2D knockdown. Data are presented as the mean ± SD, n=3, and were analysed using a two-tailed Student's t-test or one-way ANOVA. *P<0.05 and **P<0.01 vs. siNC, siRNA negative control; MTX, methotrexate; HSH2D, haematopoietic SH2 domain containing.
    Figure Legend Snippet: HSH2D is essential for MTX resistance of HuT-78 cells. (A) Silencing efficacy was evaluated using reverse transcription-quantitative PCR after transfection of two siRNAs and a NC of HSH2D. (B) Knockdown of HSH2D promoted apoptosis induced by MTX treatment in both MTX-resistant cell lines. (C) IC 50 value of MTX was detected for both sensitive and resistant cells using a Cell Counting Kit-8 assay. (D) EdU results of the cell proliferation of drug resistant strains with HSH2D knockdown. (E) Flow cytometry was used to detected (F) the cell cycle in drug resistant strains with HSH2D knockdown. Data are presented as the mean ± SD, n=3, and were analysed using a two-tailed Student's t-test or one-way ANOVA. *P<0.05 and **P<0.01 vs. siNC, siRNA negative control; MTX, methotrexate; HSH2D, haematopoietic SH2 domain containing.

    Techniques Used: Reverse Transcription, Real-time Polymerase Chain Reaction, Transfection, Knockdown, Cell Counting, Flow Cytometry, Two Tailed Test, Negative Control

    Related Articles

    Centrifugation:

    Article Title: Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells
    Article Snippet: .. Cells were then trypsinized, and harvested via centrifugation at 800 × g (4°C), and stained with 10 μl of Annexin V-FITC and 5 μl of propidium iodide using an apoptosis detection kit (Beyotime Biotechnology). .. Cells were analyzed on a Beckman Coulter (BC) FC 500 flow cytometer using the CXP software (version 2.2) for data acquisition and analysis.

    Staining:

    Article Title: Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells
    Article Snippet: .. Cells were then trypsinized, and harvested via centrifugation at 800 × g (4°C), and stained with 10 μl of Annexin V-FITC and 5 μl of propidium iodide using an apoptosis detection kit (Beyotime Biotechnology). .. Cells were analyzed on a Beckman Coulter (BC) FC 500 flow cytometer using the CXP software (version 2.2) for data acquisition and analysis.

    Article Title: CCT5 maintains mitotic fidelity and promotes early colorectal tumorigenesis
    Article Snippet: To synchronize cells at prometaphase (M phase), cells were treated with nocodazole (100 ng/mL; Sigma-Aldrich, Cat# M1404) for 14–16 h. Rounded mitotic cells were collected by mechanical shake-off, washed three times with pre-warmed PBS to remove the drug, and replated into fresh medium to release the block. .. Apoptosis: Cells were stained using the Annexin V-FITC Apoptosis Detection Kit (Beyotime) and analyzed by flow cytometry. .. Flow Cytometry: Cells were fixed in 70% ethanol at −20°C overnight, stained with Propidium Iodide (PI) and RNase A (Beyotime), and analyzed using a BD Accuri C6 flow cytometer.

    Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition
    Article Snippet: Following centrifugation to remove debris, the hydroxyproline (HYP) content in the supernatant was measured using a HYP Content Assay Kit (BC0255, Solarbio). .. After 24 h, the cells were harvested and stained with Annexin V-FITC/PI Cell Apoptosis Detection Kit (Beyotime, China) for flow cytometer analysis. ..

    Cell Culture:

    Article Title: Combining network pharmacology and experimental validation to study the action and mechanism of brusatol against lung adenocarcinoma.
    Article Snippet: The results were analyzed using a flow cytometer (BD AccuriTM C6 Flow Cytometer, United States) and the Modifit 5.0 software. .. Initially, A549 and H1299 cells (1 × 105 cells/mL) were cultured in six-well plates for 24 h and then treated with 0, 20, 40, or 80 nM brusatol for 24 h. LUAD cells were treated following the instructions provided with the Apoptosis Detection Kit (Beyotime, C1062M), and the Annexin V-FITC and PI reagents were added to each sample and incubated for 15 min in the dark. .. The results were analyzed using a flow cytometer (BD, United States).

    Incubation:

    Article Title: Combining network pharmacology and experimental validation to study the action and mechanism of brusatol against lung adenocarcinoma.
    Article Snippet: The results were analyzed using a flow cytometer (BD AccuriTM C6 Flow Cytometer, United States) and the Modifit 5.0 software. .. Initially, A549 and H1299 cells (1 × 105 cells/mL) were cultured in six-well plates for 24 h and then treated with 0, 20, 40, or 80 nM brusatol for 24 h. LUAD cells were treated following the instructions provided with the Apoptosis Detection Kit (Beyotime, C1062M), and the Annexin V-FITC and PI reagents were added to each sample and incubated for 15 min in the dark. .. The results were analyzed using a flow cytometer (BD, United States).

    Flow Cytometry:

    Article Title: CCT5 maintains mitotic fidelity and promotes early colorectal tumorigenesis
    Article Snippet: To synchronize cells at prometaphase (M phase), cells were treated with nocodazole (100 ng/mL; Sigma-Aldrich, Cat# M1404) for 14–16 h. Rounded mitotic cells were collected by mechanical shake-off, washed three times with pre-warmed PBS to remove the drug, and replated into fresh medium to release the block. .. Apoptosis: Cells were stained using the Annexin V-FITC Apoptosis Detection Kit (Beyotime) and analyzed by flow cytometry. .. Flow Cytometry: Cells were fixed in 70% ethanol at −20°C overnight, stained with Propidium Iodide (PI) and RNase A (Beyotime), and analyzed using a BD Accuri C6 flow cytometer.

    Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition
    Article Snippet: Following centrifugation to remove debris, the hydroxyproline (HYP) content in the supernatant was measured using a HYP Content Assay Kit (BC0255, Solarbio). .. After 24 h, the cells were harvested and stained with Annexin V-FITC/PI Cell Apoptosis Detection Kit (Beyotime, China) for flow cytometer analysis. ..

    CCK-8 Assay:

    Article Title: A versatile nanoplatform for enhancing the therapeutic efficacy against low-immunogenic TNBC by inducing immunogenic cell death and MHC-I upregulation
    Article Snippet: .. Calcein-AM/PI (cell viability and cytotoxicity detection kit), CCK-8 kit, Hoechst 33342, mitochondrial membrane potential detection kit (TMRE), and Annexin V-FITC apoptosis detection kit were all purchased from Beyotime Biotechnology Co., Ltd. .. The RNA extraction kit and the qPCR-specific reverse transcription kit were obtained from Sangon Biotech (Shanghai) Co., Ltd. and Bio-Rad Laboratories (Beijing) Co., Ltd., respectively.

    Membrane:

    Article Title: A versatile nanoplatform for enhancing the therapeutic efficacy against low-immunogenic TNBC by inducing immunogenic cell death and MHC-I upregulation
    Article Snippet: .. Calcein-AM/PI (cell viability and cytotoxicity detection kit), CCK-8 kit, Hoechst 33342, mitochondrial membrane potential detection kit (TMRE), and Annexin V-FITC apoptosis detection kit were all purchased from Beyotime Biotechnology Co., Ltd. .. The RNA extraction kit and the qPCR-specific reverse transcription kit were obtained from Sangon Biotech (Shanghai) Co., Ltd. and Bio-Rad Laboratories (Beijing) Co., Ltd., respectively.

    other:

    Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion
    Article Snippet: Simply put, the treated cells were fixed at room temperature with 4 % paraformaldehyde for 30 min, permeated with 0.3 % Triton X-100 (Biosharp, China) for 5 min, and incubated in the dark at 37 °C for 60 min with the TUNEL reaction mixture.



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