resource source identifier puc19 linearized vector takara bio 638947 puc19 pssp17 (TaKaRa)
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resource source identifier puc19 linearized vector takara bio 638947 puc19 pssp17
Resource Source Identifier Puc19 Linearized Vector Takara Bio 638947 Puc19 Pssp17, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+vector/In-Fusion+Snap+Assembly+Master+Mix/pm41321633-115-2-8
Average 98 stars, based on 1112 article reviews
Resource Source Identifier Puc19 Linearized Vector Takara Bio 638947 Puc19 Pssp17, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+vector/In-Fusion+Snap+Assembly+Master+Mix/pm41321633-115-2-8
Average 98 stars, based on 1112 article reviews
resource source identifier puc19 linearized vector takara bio 638947 puc19 pssp17 - by Bioz Stars,
2026-10
98/100 stars
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Amplification:Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat Article Title: Endolysin Kgh3 possesses anti-biofilm activity and affects Klebsiella pneumoniae biofilm formation through peptidoglycan degradation Article Snippet: The genome of K. pneumoniae MGH78578 served as a template to amplify the kgh3 sequence using primers K3F and K3R containing NdeI and XhoI sites, respectively, with PrimerStar DNA polymerase (Takara Bio Inc., Kusatsu, Japan). .. The kgh3 sequence was amplified and inserted into the pET-28a vector using Article Title: Macroscopic and microscopic visualization of Pseudomonas syringae pv. tabaci infection using complementary bioluminescent and fluorescent reporter systems Article Snippet: The nptII promoter was also amplified by PCR from pDSK, fused to sgfp amplified from pGWB5, and inserted into the BamHI site of pK18IP using NEBuilder. .. The gltA promoter was amplified by PCR from the Pta genome, fused to sgfp amplified from pGWB5 (Nakagawa et al. 2007), and inserted into the BamHI site of pK18IP using Polymerase Chain Reaction:Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat Article Title: Macroscopic and microscopic visualization of Pseudomonas syringae pv. tabaci infection using complementary bioluminescent and fluorescent reporter systems Article Snippet: The nptII promoter was also amplified by PCR from pDSK, fused to sgfp amplified from pGWB5, and inserted into the BamHI site of pK18IP using NEBuilder. .. The gltA promoter was amplified by PCR from the Pta genome, fused to sgfp amplified from pGWB5 (Nakagawa et al. 2007), and inserted into the BamHI site of pK18IP using Plasmid Preparation:Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat Article Title: Repressive roles of Erf and Elk in FGF-regulated neural development in Ciona intestinalis Article Snippet: .. The Article Title: Endolysin Kgh3 possesses anti-biofilm activity and affects Klebsiella pneumoniae biofilm formation through peptidoglycan degradation Article Snippet: The genome of K. pneumoniae MGH78578 served as a template to amplify the kgh3 sequence using primers K3F and K3R containing NdeI and XhoI sites, respectively, with PrimerStar DNA polymerase (Takara Bio Inc., Kusatsu, Japan). .. The kgh3 sequence was amplified and inserted into the pET-28a vector using Article Title: Proteostasis engineering of glutamine synthetase for stringent, drug-free selection in Chinese hamster ovary cells Article Snippet: The sequence for CHO ubiquitin was amplified from cDNA of CHO-K1 cells (ATCC CCL-61), and the ARE sequence (5′-TTA TTT ATT TAT TAT TTA TTT ATT -3′) was generated via PCR (Koh et al. 2019). .. All fragments were inserted into the vector using the Article Title: Signal peptide engineering of a novel M4 family keratinase and the action mechanism in promoting pepper tolerance to salt stress through KerJY23-hydrolyzed feather waste Article Snippet: .. The pBE-S vector was linearized using restriction endonucleases of Eag I and Xba I, and seamless cloning was conducted by using Article Title: A phage-encoded host transcription modulator, Htm, reprograms the transcriptome of ESBL Escherichia coli Article Snippet: The amplicon sizes were verified on a 1% agarose gel and the PCR products were purified using the GeneJet PCR Purification Kit (Thermo Scientific). .. The vector with the correct insert was constructed using the Cloning:Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat Article Title: Repressive roles of Erf and Elk in FGF-regulated neural development in Ciona intestinalis Article Snippet: .. The Article Title: Signal peptide engineering of a novel M4 family keratinase and the action mechanism in promoting pepper tolerance to salt stress through KerJY23-hydrolyzed feather waste Article Snippet: .. The pBE-S vector was linearized using restriction endonucleases of Eag I and Xba I, and seamless cloning was conducted by using Sequencing:Article Title: Endolysin Kgh3 possesses anti-biofilm activity and affects Klebsiella pneumoniae biofilm formation through peptidoglycan degradation Article Snippet: The genome of K. pneumoniae MGH78578 served as a template to amplify the kgh3 sequence using primers K3F and K3R containing NdeI and XhoI sites, respectively, with PrimerStar DNA polymerase (Takara Bio Inc., Kusatsu, Japan). .. The kgh3 sequence was amplified and inserted into the pET-28a vector using Construct:Article Title: A phage-encoded host transcription modulator, Htm, reprograms the transcriptome of ESBL Escherichia coli Article Snippet: The amplicon sizes were verified on a 1% agarose gel and the PCR products were purified using the GeneJet PCR Purification Kit (Thermo Scientific). .. The vector with the correct insert was constructed using the Transformation Assay:Article Title: A phage-encoded host transcription modulator, Htm, reprograms the transcriptome of ESBL Escherichia coli Article Snippet: The amplicon sizes were verified on a 1% agarose gel and the PCR products were purified using the GeneJet PCR Purification Kit (Thermo Scientific). .. The vector with the correct insert was constructed using the |