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packaging plasmids pvsvg  (ATCC)


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    ATCC packaging plasmids pvsvg
    KEY RESOURCES TABLE
    Packaging Plasmids Pvsvg, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software+package+based+on+the+contin+algorithm/TF-1/pmc07170217-461-36-33
    Average 97 stars, based on 1641 article reviews
    packaging plasmids pvsvg - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Mitochondrial Pyruvate Carrier 1 Promotes Peripheral T Cell Homeostasis through Metabolic Regulation of Thymic Development"

    Article Title: Mitochondrial Pyruvate Carrier 1 Promotes Peripheral T Cell Homeostasis through Metabolic Regulation of Thymic Development

    Journal: Cell reports

    doi: 10.1016/j.celrep.2020.02.042

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Staining, Plasmid Preparation, Recombinant, CRISPR, Construct, Software

    Related Articles

    Concentration Assay:

    Article Title: JAK inhibitor compounds, methods for synthesizing same and use thereof
    Article Snippet: .. -pSTAT3 assay (MSD Phospho-STAT3 (Tyr705) kit, K150SVD): TF-1 cells (CRL-2003, ATCC) were starved at high concentration (15×106 cells/flask), in T75 flasks containing reduced-serum and phenol re-free Opti-MEM (Thermofisher, 11058-021). ..

    Article Title: JAK inhibitor compounds, method for synthesizing same and use thereof
    Article Snippet: .. -pSTAT3 assay (MSD Phospho-STAT3 (Tyr705) kit, K150SVD): TF-1 cells (CRL-2003, ATCC) were starved at high concentration (15×106 cells/flask), in T75 flasks containing reduced-serum and phenol re-free Opti-MEM (Thermofisher, 11058-021). ..

    Cell Culture:

    Article Title: International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equity
    Article Snippet: Vero E6 (ATCC; CRL-1586) cells were cultured in minimum essential medium (Gibco). .. TF-1 (ATCC; CRL-2003) cells were cultured in RPMI 1640 medium (Gibco). .. Primary human CD3 + T cells (ImmunoCult) were cultured using ImmunoCult-XF T cell expansion medium (STEMCELL Technologies).

    Article Title: Antibody for binding to interleukin 4 receptor
    Article Snippet: .. Cell Culture TF-1 cells (ATCC: CRL-2003TM) frozen in liquid nitrogen were taken out, shaken in a 37° C. water bath gently to dissolve quickly. .. The dissolved cell suspension was transferred to a 15 ml centrifuge tube, and then 1640 medium (Hyclone, Catalog Number: SH30809.01B) was added therein to 10 ml.

    Virus:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Recombinant:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Lysis:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Protease Inhibitor:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Kinase Assay:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Viability Assay:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Mutagenesis:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Expressing:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Plasmid Preparation:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Retroviral:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..

    Software:

    Article Title: Constraining regulatory domain dynamics of the Src kinase Fgr increases ATP-site inhibitor sensitivity and impairs bone marrow engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fgr rabbit polyclonal Ab Cell Signaling Technologies # 2755; RRID:AB_2246957 Src pY416 monoclonal Ab Millipore Sigma # 05-677; RRID:AB_309898 Mouse monoclonal anti-Actin, clone C4 Millipore Sigma # MAB1501R; RRID:AB_2223041 IRDye 680LT Donkey anti-Mouse IgG LI-COR # 926-68022: RRID:AB_10715072 IRDye 800CW donkey anti-rabbit Ab LI-COR # 926-32213; RRID:AB_621848 Human anti-CD45-FITC conjugate BD Biosciences # 555482, RRID:AB_395874 Human anti-CD33-APC conjugate BD Biosciences # 340474, RRID:AB_400518 Bacterial and virus strains E. coli DH5α Thermo Fisher # 18265017 E. coli BL21 (DE3) pLysS Thermo Fisher # C602003 Chemicals, peptides, and recombinant proteins Puromycin Millipore Sigma # P9620 Cell Lysis Buffer Cell Signaling Technologies # 9803 Antibiotic-Antimycotic Life Technologies # 15240062 Protease Inhibitor Cocktail Millipore Sigma # 11697498001 Human GM-CSF Life Technologies # 15240062 VSL12 SH3-binding peptide U. Pittsburgh Peptide Core Custom synthesis YIYGSFK Fgr substrate peptide U. Pittsburgh Peptide Core Custom synthesis A-419259 kinase inhibitor Cayman Chemical #18168 TL02-59 kinase inhibitor A Chem Tek Custom synthesis IVISbrite D-Luciferin Bioluminescent Substrate Revvity # 770504 Streptavidin M-270 Dynabeads Thermo Fisher # 65305 Critical commercial assays ADP Quest kinetic kinase assay Eurofins/DiscoverX # 90-0071 CellTiter-Blue cell viability assay Promega # G8080 QuikChange II site directed mutagenesis kit Agilent # 200523 Deposited data Fgr SH3-SH2-linker, WT crystal structure This study PDB: 10DT Fgr SH3-SH2-HAL1 crystal structure This study PDB: 10FS Fgr SH3-SH2-HAL2 crystal structure This study PDB: 10FV Fgr SH3-SH2-HAL3 crystal structure This study PDB: 10GA Experimental models: Cell lines TF-1 Cells ATCC # CRL-2003 293T Cells ATCC # CRL-3216 Experimental models: Organisms NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) immunodeficient mice Jackson Laboratories # 005557 Recombinant DNA pET-28a bacterial expression plasmid Millipore Sigma # 69864 pET-DUET bacterial expression vector Millipore Sigma # 71146 pACYC-Skp-GroEL/ES expression vector Addgene # 83922 pMSCVpuro retroviral vector Takara # 634401 Software and algorithms Prism for statistical analysis, v 10.1 GraphPad https://www.graphpad.com/ (Continued on next page) Cell Reports 45, 117551, June 23, 2026 11 ..



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