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genbank data base
Genbank Data Base, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+based+on+the+contin+algorithm/BRD4+(LONG+ISOFORM)_pLX307+(Plasmid+%2398318)/pmc09617197-176-28-41
Average 93 stars, based on 25 article reviews
Genbank Data Base, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+based+on+the+contin+algorithm/BRD4+(LONG+ISOFORM)_pLX307+(Plasmid+%2398318)/pmc09617197-176-28-41
Average 93 stars, based on 25 article reviews
genbank data base - by Bioz Stars,
2026-09
93/100 stars
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1) Product Images from "Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells"
Article Title: Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells
Journal: STAR Protocols
doi: 10.1016/j.xpro.2022.101785
Figure Legend Snippet:
Techniques Used: Virus, Recombinant, Cloning, Gel Extraction, Plasmid Preparation, Sequencing, Over Expression, Expressing, Software, Imaging
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Mutagenesis:Article Title: BRD4 binds to active cranial neural crest enhancers to regulate RUNX2 activity during osteoblast differentiation. Article Snippet: O9-1 cranial neural crest cells were maintained in culture as described (Ishii et al. 2012) and grown on Matrigel coated plates (Corning 356234 or Biotechne 3432-005-01). .. To create D ev el o pm en t • A cc ep te d m an us cr Transfection:Article Title: BRD4 binds to active cranial neural crest enhancers to regulate RUNX2 activity during osteoblast differentiation. Article Snippet: O9-1 cranial neural crest cells were maintained in culture as described (Ishii et al. 2012) and grown on Matrigel coated plates (Corning 356234 or Biotechne 3432-005-01). .. To create D ev el o pm en t • A cc ep te d m an us cr Single Cell:Article Title: BRD4 binds to active cranial neural crest enhancers to regulate RUNX2 activity during osteoblast differentiation. Article Snippet: O9-1 cranial neural crest cells were maintained in culture as described (Ishii et al. 2012) and grown on Matrigel coated plates (Corning 356234 or Biotechne 3432-005-01). .. To create D ev el o pm en t • A cc ep te d m an us cr Fluorescence:Article Title: BRD4 binds to active cranial neural crest enhancers to regulate RUNX2 activity during osteoblast differentiation. Article Snippet: O9-1 cranial neural crest cells were maintained in culture as described (Ishii et al. 2012) and grown on Matrigel coated plates (Corning 356234 or Biotechne 3432-005-01). .. To create D ev el o pm en t • A cc ep te d m an us cr Amplification:Article Title: Identification of a SNAI1 enhancer RNA that drives cancer cell plasticity. Article Snippet: The inducible vector for SNAI1 ectopic expression was generated Nature Communications | (2025) 16:2890 11 using Gateway cloning into the pLIX-403 vector (Addgene; 41395). .. Article Title: Increased expression of BRD4 isoforms long (BRD4-L) and short (BRD4-S) promotes chemotherapy resistance in high-grade serous ovarian carcinoma Article Snippet: .. The BRD4-L and Polymerase Chain Reaction:Article Title: Identification of a SNAI1 enhancer RNA that drives cancer cell plasticity. Article Snippet: The inducible vector for SNAI1 ectopic expression was generated Nature Communications | (2025) 16:2890 11 using Gateway cloning into the pLIX-403 vector (Addgene; 41395). .. Article Title: Increased expression of BRD4 isoforms long (BRD4-L) and short (BRD4-S) promotes chemotherapy resistance in high-grade serous ovarian carcinoma Article Snippet: .. The BRD4-L and shRNA:Article Title: Identification of a SNAI1 enhancer RNA that drives cancer cell plasticity Article Snippet: .. We used shRNA constructs from Sigma‒Aldrich for gene knockdown: TRCN0000040031 for SMAD4 knockdown, TRCN0000199427 (#1) and TRCN0000318771 (#2) for Construct:Article Title: Identification of a SNAI1 enhancer RNA that drives cancer cell plasticity Article Snippet: .. We used shRNA constructs from Sigma‒Aldrich for gene knockdown: TRCN0000040031 for SMAD4 knockdown, TRCN0000199427 (#1) and TRCN0000318771 (#2) for Knockdown:Article Title: Identification of a SNAI1 enhancer RNA that drives cancer cell plasticity Article Snippet: .. We used shRNA constructs from Sigma‒Aldrich for gene knockdown: TRCN0000040031 for SMAD4 knockdown, TRCN0000199427 (#1) and TRCN0000318771 (#2) for CRISPR:Article Title: Identification of a SNAI1 enhancer RNA that drives cancer cell plasticity Article Snippet: .. We used shRNA constructs from Sigma‒Aldrich for gene knockdown: TRCN0000040031 for SMAD4 knockdown, TRCN0000199427 (#1) and TRCN0000318771 (#2) for Cloning:Article Title: The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma Article Snippet: Estimates of IC50 were calculated by logistic regression (GraphPad Prism). .. Plasmids, cloning, and viral transduction pInducer20 blast blast-N-BRD4-NUT-C-HA lentiviral plasmidwas created by Gibson assembly of gBlocks (synthesized by Integrated DNA Technologies, Inc.; IDT) of the Article Title: Increased expression of BRD4 isoforms long (BRD4-L) and short (BRD4-S) promotes chemotherapy resistance in high-grade serous ovarian carcinoma Article Snippet: .. The BRD4-L and Transduction:Article Title: The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma Article Snippet: Estimates of IC50 were calculated by logistic regression (GraphPad Prism). .. Plasmids, cloning, and viral transduction pInducer20 blast blast-N-BRD4-NUT-C-HA lentiviral plasmidwas created by Gibson assembly of gBlocks (synthesized by Integrated DNA Technologies, Inc.; IDT) of the Synthesized:Article Title: The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma Article Snippet: Estimates of IC50 were calculated by logistic regression (GraphPad Prism). .. Plasmids, cloning, and viral transduction pInducer20 blast blast-N-BRD4-NUT-C-HA lentiviral plasmidwas created by Gibson assembly of gBlocks (synthesized by Integrated DNA Technologies, Inc.; IDT) of the Sequencing:Article Title: The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma Article Snippet: Estimates of IC50 were calculated by logistic regression (GraphPad Prism). .. Plasmids, cloning, and viral transduction pInducer20 blast blast-N-BRD4-NUT-C-HA lentiviral plasmidwas created by Gibson assembly of gBlocks (synthesized by Integrated DNA Technologies, Inc.; IDT) of the Clone Assay:Article Title: Increased expression of BRD4 isoforms long (BRD4-L) and short (BRD4-S) promotes chemotherapy resistance in high-grade serous ovarian carcinoma Article Snippet: .. The BRD4-L and Article Title: Solid phase transitions as a solution to the genome folding paradox. Article Snippet: Ultra-long-range genomic contacts, which are key components of neuronal genome architecture, constitute a biochemical enigma.. This is because regulatory DNA elements make selective and stable contacts with DNA sequences located hundreds of kilobases away, instead of interacting with proximal sequences occupied by the exact same transcription factors.. This is exemplified in olfactory sensory neurons (OSNs), in which only a fraction of LHX2-, EBF1and LDB1-bound sites interact with each other, converging into highly selective multi-chromosomal enhancer hubs. Over Expression:Article Title: Increased expression of BRD4 isoforms long (BRD4-L) and short (BRD4-S) promotes chemotherapy resistance in high-grade serous ovarian carcinoma Article Snippet: .. The BRD4-L and Plasmid Preparation:Article Title: Increased expression of BRD4 isoforms long (BRD4-L) and short (BRD4-S) promotes chemotherapy resistance in high-grade serous ovarian carcinoma Article Snippet: .. The BRD4-L and Article Title: Solid phase transitions as a solution to the genome folding paradox. Article Snippet: Ultra-long-range genomic contacts, which are key components of neuronal genome architecture, constitute a biochemical enigma.. This is because regulatory DNA elements make selective and stable contacts with DNA sequences located hundreds of kilobases away, instead of interacting with proximal sequences occupied by the exact same transcription factors.. This is exemplified in olfactory sensory neurons (OSNs), in which only a fraction of LHX2-, EBF1and LDB1-bound sites interact with each other, converging into highly selective multi-chromosomal enhancer hubs. |