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algorithms gen5 software biotek n a metamorph 7 8 13 gataca systems n a fiji nih  (Gataca Inc)

 
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    Gataca Inc algorithms gen5 software biotek n a metamorph 7 8 13 gataca systems n a fiji nih
    Algorithms Gen5 Software Biotek N A Metamorph 7 8 13 Gataca Systems N A Fiji Nih, supplied by Gataca Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software%2C+algorithm/13+7+8+a+a+algorithms+biotek+fiji+gataca+gen5+metamorph+n+n+nih+software+systems/pm41996234-680-156-163
    Average 86 stars, based on 1 article reviews
    algorithms gen5 software biotek n a metamorph 7 8 13 gataca systems n a fiji nih - by Bioz Stars, 2026-09
    86/100 stars

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    Recombinant:

    Article Title: Dose-dependent mitochondrial H 2 O 2 signaling drives toxicity or stress adaptation and longevity in fission yeast.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primer list See Table S3 N/A Recombinant DNA p407.C169S sty1 prom::roGFP2-Tpx1.C169S Carmona et al.21 p728 sty1 prom::HA-HyPer7 de Cubas et al.31 p730 sty1 prom::MTSaco1-HA-HyPer7 de Cubas et al.31 p836 sty1 prom::IMScym1-HA-HyPer7 This study p840 sty1 prom::NLSSV40-HA-HyPer7 This study p789.NLS sty1 prom::NLSSV40-sfGFP-NLS SV40-sty1 This study p838 sty1 prom::NESPKI-HA-HyPer7 This study p699 sty1 prom::NESPKI-Rho1.C17RGFP This study AY024 nmt41x′ prom integrative Lab stock p862 sty1 prom::MBP-HyPer7 This study pRgDAO RgDAO Iria Medraño/Roberto Sitia’s labs p858′ sty1 prom::HA-RgDAO This study p868′ sty1 prom::dao1 This study p832′ sty1 prom::MTSaco1-tpx1 de Cubas et al.61 p831′ sty1 prom::MTSaco1-ctt1 de Cubas et al.61 AY1031 empty vector ura4 integrative Vjestica et al.62 p860′ sty1 prom::MTSaco1-RgDAO This study p853′ sty1 prom::MTSaco1-dao1 This study p903′ sty1 prom::RgDAO-GFP This study p904′ sty1 prom::MTSaco1-RgDAO-GFP This study p902′ sty1 prom::dao1-GFP This study p901′ sty1 prom::MTSaco1-dao1-GFP This study p906′ sty1 prom::MTSaco1-ctt1-GFP This study p907′ nop1 prom::MTSaco1-dao1 This study pAY1369 sty1 prom::MTSaco1-dao1 This study Software and algorithms Gen5 software Biotek N/A Metamorph 7.8.13 Gataca Systems N/A Fiji NIH; Schindelin 2012 https://imagej.net/software/fiji/ Image Lab Bio-Rad N/A GraphPad Prism (6.0c) GraphPad Software https://www.graphpad.com/ BioRender BioRender https://www.biorender.com/ Cell Reports 45, 117277, April 28, 2026 17 from episomal plasmids, with an average of 7–8 plasmid copies per cell.64 We used plasmids p407.C169S, p728 and p730 to allow the expression of roGFP2-Tpx1.C169, HyPer7 and MTS-HyPer7, respectively.21,31 To allow expression of IMS-targeted HyPer7, p836 was created by PCR-amplification from genomic S. pombe DNA of the first 86 codons of cym1 and insertion upstream of hyper7 in plasmid p728. p840, allowing the expression of NLSSV40-HyPer7, was obtained by restriction enzyme digestion from p789.NLS into p728. p789.NLS codes for a sty1-driven NLSSV40-sfGFP-Sty1 in an integrative AY024 backbone. p862 coding for MBP-HyPer7 was constructed by inserting the maltose-binding protein (MBP) into p728. ..

    Plasmid Preparation:

    Article Title: Dose-dependent mitochondrial H 2 O 2 signaling drives toxicity or stress adaptation and longevity in fission yeast.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primer list See Table S3 N/A Recombinant DNA p407.C169S sty1 prom::roGFP2-Tpx1.C169S Carmona et al.21 p728 sty1 prom::HA-HyPer7 de Cubas et al.31 p730 sty1 prom::MTSaco1-HA-HyPer7 de Cubas et al.31 p836 sty1 prom::IMScym1-HA-HyPer7 This study p840 sty1 prom::NLSSV40-HA-HyPer7 This study p789.NLS sty1 prom::NLSSV40-sfGFP-NLS SV40-sty1 This study p838 sty1 prom::NESPKI-HA-HyPer7 This study p699 sty1 prom::NESPKI-Rho1.C17RGFP This study AY024 nmt41x′ prom integrative Lab stock p862 sty1 prom::MBP-HyPer7 This study pRgDAO RgDAO Iria Medraño/Roberto Sitia’s labs p858′ sty1 prom::HA-RgDAO This study p868′ sty1 prom::dao1 This study p832′ sty1 prom::MTSaco1-tpx1 de Cubas et al.61 p831′ sty1 prom::MTSaco1-ctt1 de Cubas et al.61 AY1031 empty vector ura4 integrative Vjestica et al.62 p860′ sty1 prom::MTSaco1-RgDAO This study p853′ sty1 prom::MTSaco1-dao1 This study p903′ sty1 prom::RgDAO-GFP This study p904′ sty1 prom::MTSaco1-RgDAO-GFP This study p902′ sty1 prom::dao1-GFP This study p901′ sty1 prom::MTSaco1-dao1-GFP This study p906′ sty1 prom::MTSaco1-ctt1-GFP This study p907′ nop1 prom::MTSaco1-dao1 This study pAY1369 sty1 prom::MTSaco1-dao1 This study Software and algorithms Gen5 software Biotek N/A Metamorph 7.8.13 Gataca Systems N/A Fiji NIH; Schindelin 2012 https://imagej.net/software/fiji/ Image Lab Bio-Rad N/A GraphPad Prism (6.0c) GraphPad Software https://www.graphpad.com/ BioRender BioRender https://www.biorender.com/ Cell Reports 45, 117277, April 28, 2026 17 from episomal plasmids, with an average of 7–8 plasmid copies per cell.64 We used plasmids p407.C169S, p728 and p730 to allow the expression of roGFP2-Tpx1.C169, HyPer7 and MTS-HyPer7, respectively.21,31 To allow expression of IMS-targeted HyPer7, p836 was created by PCR-amplification from genomic S. pombe DNA of the first 86 codons of cym1 and insertion upstream of hyper7 in plasmid p728. p840, allowing the expression of NLSSV40-HyPer7, was obtained by restriction enzyme digestion from p789.NLS into p728. p789.NLS codes for a sty1-driven NLSSV40-sfGFP-Sty1 in an integrative AY024 backbone. p862 coding for MBP-HyPer7 was constructed by inserting the maltose-binding protein (MBP) into p728. ..

    Software:

    Article Title: Dose-dependent mitochondrial H 2 O 2 signaling drives toxicity or stress adaptation and longevity in fission yeast.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primer list See Table S3 N/A Recombinant DNA p407.C169S sty1 prom::roGFP2-Tpx1.C169S Carmona et al.21 p728 sty1 prom::HA-HyPer7 de Cubas et al.31 p730 sty1 prom::MTSaco1-HA-HyPer7 de Cubas et al.31 p836 sty1 prom::IMScym1-HA-HyPer7 This study p840 sty1 prom::NLSSV40-HA-HyPer7 This study p789.NLS sty1 prom::NLSSV40-sfGFP-NLS SV40-sty1 This study p838 sty1 prom::NESPKI-HA-HyPer7 This study p699 sty1 prom::NESPKI-Rho1.C17RGFP This study AY024 nmt41x′ prom integrative Lab stock p862 sty1 prom::MBP-HyPer7 This study pRgDAO RgDAO Iria Medraño/Roberto Sitia’s labs p858′ sty1 prom::HA-RgDAO This study p868′ sty1 prom::dao1 This study p832′ sty1 prom::MTSaco1-tpx1 de Cubas et al.61 p831′ sty1 prom::MTSaco1-ctt1 de Cubas et al.61 AY1031 empty vector ura4 integrative Vjestica et al.62 p860′ sty1 prom::MTSaco1-RgDAO This study p853′ sty1 prom::MTSaco1-dao1 This study p903′ sty1 prom::RgDAO-GFP This study p904′ sty1 prom::MTSaco1-RgDAO-GFP This study p902′ sty1 prom::dao1-GFP This study p901′ sty1 prom::MTSaco1-dao1-GFP This study p906′ sty1 prom::MTSaco1-ctt1-GFP This study p907′ nop1 prom::MTSaco1-dao1 This study pAY1369 sty1 prom::MTSaco1-dao1 This study Software and algorithms Gen5 software Biotek N/A Metamorph 7.8.13 Gataca Systems N/A Fiji NIH; Schindelin 2012 https://imagej.net/software/fiji/ Image Lab Bio-Rad N/A GraphPad Prism (6.0c) GraphPad Software https://www.graphpad.com/ BioRender BioRender https://www.biorender.com/ Cell Reports 45, 117277, April 28, 2026 17 from episomal plasmids, with an average of 7–8 plasmid copies per cell.64 We used plasmids p407.C169S, p728 and p730 to allow the expression of roGFP2-Tpx1.C169, HyPer7 and MTS-HyPer7, respectively.21,31 To allow expression of IMS-targeted HyPer7, p836 was created by PCR-amplification from genomic S. pombe DNA of the first 86 codons of cym1 and insertion upstream of hyper7 in plasmid p728. p840, allowing the expression of NLSSV40-HyPer7, was obtained by restriction enzyme digestion from p789.NLS into p728. p789.NLS codes for a sty1-driven NLSSV40-sfGFP-Sty1 in an integrative AY024 backbone. p862 coding for MBP-HyPer7 was constructed by inserting the maltose-binding protein (MBP) into p728. ..

    Expressing:

    Article Title: Dose-dependent mitochondrial H 2 O 2 signaling drives toxicity or stress adaptation and longevity in fission yeast.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primer list See Table S3 N/A Recombinant DNA p407.C169S sty1 prom::roGFP2-Tpx1.C169S Carmona et al.21 p728 sty1 prom::HA-HyPer7 de Cubas et al.31 p730 sty1 prom::MTSaco1-HA-HyPer7 de Cubas et al.31 p836 sty1 prom::IMScym1-HA-HyPer7 This study p840 sty1 prom::NLSSV40-HA-HyPer7 This study p789.NLS sty1 prom::NLSSV40-sfGFP-NLS SV40-sty1 This study p838 sty1 prom::NESPKI-HA-HyPer7 This study p699 sty1 prom::NESPKI-Rho1.C17RGFP This study AY024 nmt41x′ prom integrative Lab stock p862 sty1 prom::MBP-HyPer7 This study pRgDAO RgDAO Iria Medraño/Roberto Sitia’s labs p858′ sty1 prom::HA-RgDAO This study p868′ sty1 prom::dao1 This study p832′ sty1 prom::MTSaco1-tpx1 de Cubas et al.61 p831′ sty1 prom::MTSaco1-ctt1 de Cubas et al.61 AY1031 empty vector ura4 integrative Vjestica et al.62 p860′ sty1 prom::MTSaco1-RgDAO This study p853′ sty1 prom::MTSaco1-dao1 This study p903′ sty1 prom::RgDAO-GFP This study p904′ sty1 prom::MTSaco1-RgDAO-GFP This study p902′ sty1 prom::dao1-GFP This study p901′ sty1 prom::MTSaco1-dao1-GFP This study p906′ sty1 prom::MTSaco1-ctt1-GFP This study p907′ nop1 prom::MTSaco1-dao1 This study pAY1369 sty1 prom::MTSaco1-dao1 This study Software and algorithms Gen5 software Biotek N/A Metamorph 7.8.13 Gataca Systems N/A Fiji NIH; Schindelin 2012 https://imagej.net/software/fiji/ Image Lab Bio-Rad N/A GraphPad Prism (6.0c) GraphPad Software https://www.graphpad.com/ BioRender BioRender https://www.biorender.com/ Cell Reports 45, 117277, April 28, 2026 17 from episomal plasmids, with an average of 7–8 plasmid copies per cell.64 We used plasmids p407.C169S, p728 and p730 to allow the expression of roGFP2-Tpx1.C169, HyPer7 and MTS-HyPer7, respectively.21,31 To allow expression of IMS-targeted HyPer7, p836 was created by PCR-amplification from genomic S. pombe DNA of the first 86 codons of cym1 and insertion upstream of hyper7 in plasmid p728. p840, allowing the expression of NLSSV40-HyPer7, was obtained by restriction enzyme digestion from p789.NLS into p728. p789.NLS codes for a sty1-driven NLSSV40-sfGFP-Sty1 in an integrative AY024 backbone. p862 coding for MBP-HyPer7 was constructed by inserting the maltose-binding protein (MBP) into p728. ..

    Polymerase Chain Reaction:

    Article Title: Dose-dependent mitochondrial H 2 O 2 signaling drives toxicity or stress adaptation and longevity in fission yeast.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primer list See Table S3 N/A Recombinant DNA p407.C169S sty1 prom::roGFP2-Tpx1.C169S Carmona et al.21 p728 sty1 prom::HA-HyPer7 de Cubas et al.31 p730 sty1 prom::MTSaco1-HA-HyPer7 de Cubas et al.31 p836 sty1 prom::IMScym1-HA-HyPer7 This study p840 sty1 prom::NLSSV40-HA-HyPer7 This study p789.NLS sty1 prom::NLSSV40-sfGFP-NLS SV40-sty1 This study p838 sty1 prom::NESPKI-HA-HyPer7 This study p699 sty1 prom::NESPKI-Rho1.C17RGFP This study AY024 nmt41x′ prom integrative Lab stock p862 sty1 prom::MBP-HyPer7 This study pRgDAO RgDAO Iria Medraño/Roberto Sitia’s labs p858′ sty1 prom::HA-RgDAO This study p868′ sty1 prom::dao1 This study p832′ sty1 prom::MTSaco1-tpx1 de Cubas et al.61 p831′ sty1 prom::MTSaco1-ctt1 de Cubas et al.61 AY1031 empty vector ura4 integrative Vjestica et al.62 p860′ sty1 prom::MTSaco1-RgDAO This study p853′ sty1 prom::MTSaco1-dao1 This study p903′ sty1 prom::RgDAO-GFP This study p904′ sty1 prom::MTSaco1-RgDAO-GFP This study p902′ sty1 prom::dao1-GFP This study p901′ sty1 prom::MTSaco1-dao1-GFP This study p906′ sty1 prom::MTSaco1-ctt1-GFP This study p907′ nop1 prom::MTSaco1-dao1 This study pAY1369 sty1 prom::MTSaco1-dao1 This study Software and algorithms Gen5 software Biotek N/A Metamorph 7.8.13 Gataca Systems N/A Fiji NIH; Schindelin 2012 https://imagej.net/software/fiji/ Image Lab Bio-Rad N/A GraphPad Prism (6.0c) GraphPad Software https://www.graphpad.com/ BioRender BioRender https://www.biorender.com/ Cell Reports 45, 117277, April 28, 2026 17 from episomal plasmids, with an average of 7–8 plasmid copies per cell.64 We used plasmids p407.C169S, p728 and p730 to allow the expression of roGFP2-Tpx1.C169, HyPer7 and MTS-HyPer7, respectively.21,31 To allow expression of IMS-targeted HyPer7, p836 was created by PCR-amplification from genomic S. pombe DNA of the first 86 codons of cym1 and insertion upstream of hyper7 in plasmid p728. p840, allowing the expression of NLSSV40-HyPer7, was obtained by restriction enzyme digestion from p789.NLS into p728. p789.NLS codes for a sty1-driven NLSSV40-sfGFP-Sty1 in an integrative AY024 backbone. p862 coding for MBP-HyPer7 was constructed by inserting the maltose-binding protein (MBP) into p728. ..

    Construct:

    Article Title: Dose-dependent mitochondrial H 2 O 2 signaling drives toxicity or stress adaptation and longevity in fission yeast.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primer list See Table S3 N/A Recombinant DNA p407.C169S sty1 prom::roGFP2-Tpx1.C169S Carmona et al.21 p728 sty1 prom::HA-HyPer7 de Cubas et al.31 p730 sty1 prom::MTSaco1-HA-HyPer7 de Cubas et al.31 p836 sty1 prom::IMScym1-HA-HyPer7 This study p840 sty1 prom::NLSSV40-HA-HyPer7 This study p789.NLS sty1 prom::NLSSV40-sfGFP-NLS SV40-sty1 This study p838 sty1 prom::NESPKI-HA-HyPer7 This study p699 sty1 prom::NESPKI-Rho1.C17RGFP This study AY024 nmt41x′ prom integrative Lab stock p862 sty1 prom::MBP-HyPer7 This study pRgDAO RgDAO Iria Medraño/Roberto Sitia’s labs p858′ sty1 prom::HA-RgDAO This study p868′ sty1 prom::dao1 This study p832′ sty1 prom::MTSaco1-tpx1 de Cubas et al.61 p831′ sty1 prom::MTSaco1-ctt1 de Cubas et al.61 AY1031 empty vector ura4 integrative Vjestica et al.62 p860′ sty1 prom::MTSaco1-RgDAO This study p853′ sty1 prom::MTSaco1-dao1 This study p903′ sty1 prom::RgDAO-GFP This study p904′ sty1 prom::MTSaco1-RgDAO-GFP This study p902′ sty1 prom::dao1-GFP This study p901′ sty1 prom::MTSaco1-dao1-GFP This study p906′ sty1 prom::MTSaco1-ctt1-GFP This study p907′ nop1 prom::MTSaco1-dao1 This study pAY1369 sty1 prom::MTSaco1-dao1 This study Software and algorithms Gen5 software Biotek N/A Metamorph 7.8.13 Gataca Systems N/A Fiji NIH; Schindelin 2012 https://imagej.net/software/fiji/ Image Lab Bio-Rad N/A GraphPad Prism (6.0c) GraphPad Software https://www.graphpad.com/ BioRender BioRender https://www.biorender.com/ Cell Reports 45, 117277, April 28, 2026 17 from episomal plasmids, with an average of 7–8 plasmid copies per cell.64 We used plasmids p407.C169S, p728 and p730 to allow the expression of roGFP2-Tpx1.C169, HyPer7 and MTS-HyPer7, respectively.21,31 To allow expression of IMS-targeted HyPer7, p836 was created by PCR-amplification from genomic S. pombe DNA of the first 86 codons of cym1 and insertion upstream of hyper7 in plasmid p728. p840, allowing the expression of NLSSV40-HyPer7, was obtained by restriction enzyme digestion from p789.NLS into p728. p789.NLS codes for a sty1-driven NLSSV40-sfGFP-Sty1 in an integrative AY024 backbone. p862 coding for MBP-HyPer7 was constructed by inserting the maltose-binding protein (MBP) into p728. ..



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