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wavemetrics inc software and algorithms igor pro
Software And Algorithms Igor Pro, supplied by wavemetrics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software%2C+algorithm+igor+pro/igor+software/pm40460202-220-3-11
Average 90 stars, based on 1 article reviews
software and algorithms igor pro - by Bioz Stars, 2026-09
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Software:

Article Title: Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. ..

Article Title: rHuEPO-α Biosensing by Porous Silicon Integrated into Microchannels via Reflectometric Interference Fourier Transform Spectroscopy.
Article Snippet: The performance of biosensors integrated into microfluidic devices is strongly influenced by the geometry of the microchannels.. In this study, the effect of microchannel height, as the most significant geometrical factor, on the sensitivity of the porous silicon (PSi)-based biosensor was investigated for the detection of recombinant human erythropoietin-α (rHuEPO-α) using reflectometric interference Fourier transform spectroscopy.. Microchannels were fabricated using the soft lithography method, and a specific aptamer for rHuEPO-α was employed as the bioreceptor.

Article Title: Photoelectron circular dichroism of aqueous-phase alanine
Article Snippet: .. All data reduction was performed using the Igor Pro analysis software (Wavemetrics, version 9). ..

Article Title: An Anatomical and Physiological Basis for Flexible Coincidence Detection in the Auditory System
Article Snippet: .. (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug Strychnine Sigma Aldrich S0532, CAS: 57- 24- 9 Software, algorithm Imaris Oxford Instruments Imaris Software, algorithm ImageJ https://imagej.net/ij/ ImageJ Software, algorithm pClamp9 Molecular Devices pClamp9 Software, algorithm Igor Pro Wavemetrics Igor Pro Software, algorithm NeuroMatic http://www.neuromatic.thinkrandom.com/ NeuroMatic Software, algorithm NEURON simulation environment https://neuron.yale.edu/neuron/ NEURON simulation environment Software, algorithm NEURON simulation code https://modeldb.science/2018259 NEURON simulation code Software, algorithm Imaris Oxford Instruments Imaris Continued Animal use and transgenic mouse lines All procedures were approved by and conducted in accordance with Harvard Medical School Institutional Animal Care and Use Committee (Protocol Number IS00000067- 9). .. Male and female mice (Mus musculus) were bred on a C57BL/6 background at the Harvard Center for Comparative Medicine or obtained from Jackson Laboratories.

Article Title: Odontoblasts in developing, mature and aging rat teeth have multiple phenotypes that variably express all nine voltage-gated sodium channels
Article Snippet: .. Immunohistochemistry groups were compared using the same pixel intensity range (0–250) and Igor software (Wavemetrics). ..

Article Title: Method for preparing a coated article
Article Snippet: .. Data analysis was handled using the Igor Pro software package available from WaveMetrics and proprietary code developed within Igor Pro. ..

High-Pressure Homogenizer:

Article Title: Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. ..

Bicinchoninic Acid Protein Assay:

Article Title: Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. ..

Polymerase Chain Reaction:

Article Title: Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. ..

Chromatography:

Article Title: Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. ..

Microscopy:

Article Title: Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. ..

Sonication:

Article Title: Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuA.
Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Igor Pro https://www.wavemetrics.com Version 9.0.2 Other Applikon bioreactor Getinge N/A High-pressure homogenizer HPL6 Maximator GmbH N/A Potter-Elvehjem PTFE pestle and glass tube (45 mL) Sigma-Aldrich P7984 Potter-Elvehjem PTFE pestle and glass tube (8 mL) Sigma-Aldrich P7859 Pierce BCA Protein Assay Kit Thermo Scientific 23225 Rotary evaporator BUCHI N/A Teflon tape N/A N/A Milli-Q system (ultrapure lab water system) Merck Millipore N/A PCR machine N/A N/A Nanodrop N/A N/A NucleoSpin Gel & PCR Clean-up Macherey-Nagel 740609 C-Trap Dymo (with short tether piezo tracking) Lumicks N/A NGC chromatography system Bio-Rad N/A Poly-Prep chromatography column (10 mL) Bio-Rad 7311553 Superdex 200 Increase 10/300 GL Sigma-Aldrich GE28-9909-44 High-speed centrifuge: Avanti J-20 XP Beckman Coulter N/A Table-top ultracentrifuge: Optima Max-XP Beckman Coulter 393315 Ultracentrifuge: Optima XE-90 Beckman Coulter A94471 JLA-9.1000 fixed-angle aluminum rotor Beckman Coulter 336754 JA-25.50 fixed-angle rotor Beckman Coulter 363055 Type 50.2 Ti fixed-angle rotor Beckman Coulter 337901 Type 45 Ti fixed-angle titanium rotor Beckman Coulter 339160 MLA-80 fixed-angle rotor package Beckman Coulter 367096 Ultrasonic batch Bandlein N/A Q125 Probe sonicator QSonica 5323–15 Probe tip, 5/64 ′′ , 200 μL–5mL QSonica 5323–09 Microscope slide (26 x 76) mm, 1 mm thickness Knittel Glass N/A Cover-slide (22 x 22) mm, 175 μm thickness Knittel Glass N/A Parafilm N/A N/A Heating plate N/A N/A STAR Protocols 6, 103869, June 20, 2025 11 Note: The lipid stock is diluted to 1 mL, since this is the minimum volume required for our sonication setup. c. Tip-sonicate the lipids in an ice-water bath for 8 cycles: 15 seconds on, 45 seconds off, ampli- tude 77 μm. ..

other:

Article Title: Residue-specific pathways in peptide fragmentation: The role of aromatic side chain in a3 ion formation from b3 ion
Article Snippet: Peptide fragmentation chemistry is essential for the sequence elucidation of proteins through tandem mass spectrometry (MS/MS).. In this study, we examine the gas-phase fragmentation of b3 ions from model tripeptides under low-energy CID conditions, focusing on the pathway leading to the stable formation of a3 ions from b3 ions.. The study utilized C-terminal amidated model tripeptides, including YGG-NH2, GYG-NH2, and GGX-NH2, where X represents D, E, H, Q, C, S, F, and Y.

Article Title: Enhancing Differentiation of Oxygenated Organic Aerosol: A Machine Learning Approach to Distinguish Local and Transboundary Pollution
Article Snippet: The raw data set collected by Q-ACSM was processed using standard data analysis software (version 1.6.1.1) based on Igor Pro (Wavemetrics Inc.) to obtain the mass concentration of NR-PM 1 species.

Transformation Assay:

Article Title: rHuEPO-α Biosensing by Porous Silicon Integrated into Microchannels via Reflectometric Interference Fourier Transform Spectroscopy.
Article Snippet: The performance of biosensors integrated into microfluidic devices is strongly influenced by the geometry of the microchannels.. In this study, the effect of microchannel height, as the most significant geometrical factor, on the sensitivity of the porous silicon (PSi)-based biosensor was investigated for the detection of recombinant human erythropoietin-α (rHuEPO-α) using reflectometric interference Fourier transform spectroscopy.. Microchannels were fabricated using the soft lithography method, and a specific aptamer for rHuEPO-α was employed as the bioreceptor.

Transgenic Assay:

Article Title: An Anatomical and Physiological Basis for Flexible Coincidence Detection in the Auditory System
Article Snippet: .. (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug Strychnine Sigma Aldrich S0532, CAS: 57- 24- 9 Software, algorithm Imaris Oxford Instruments Imaris Software, algorithm ImageJ https://imagej.net/ij/ ImageJ Software, algorithm pClamp9 Molecular Devices pClamp9 Software, algorithm Igor Pro Wavemetrics Igor Pro Software, algorithm NeuroMatic http://www.neuromatic.thinkrandom.com/ NeuroMatic Software, algorithm NEURON simulation environment https://neuron.yale.edu/neuron/ NEURON simulation environment Software, algorithm NEURON simulation code https://modeldb.science/2018259 NEURON simulation code Software, algorithm Imaris Oxford Instruments Imaris Continued Animal use and transgenic mouse lines All procedures were approved by and conducted in accordance with Harvard Medical School Institutional Animal Care and Use Committee (Protocol Number IS00000067- 9). .. Male and female mice (Mus musculus) were bred on a C57BL/6 background at the Harvard Center for Comparative Medicine or obtained from Jackson Laboratories.

Immunohistochemistry:

Article Title: Odontoblasts in developing, mature and aging rat teeth have multiple phenotypes that variably express all nine voltage-gated sodium channels
Article Snippet: .. Immunohistochemistry groups were compared using the same pixel intensity range (0–250) and Igor software (Wavemetrics). ..



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