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smart seq ht kit 10x lysis buffer  (TaKaRa)


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    Structured Review

    TaKaRa smart seq ht kit 10x lysis buffer
    Smart Seq Ht Kit 10x Lysis Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 3101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart-seq+buffer/Lysis+Buffer/pm40595542-446-22-29
    Average 97 stars, based on 3101 article reviews
    smart seq ht kit 10x lysis buffer - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Protocol for purifying and sequencing cell subpopulations based on RNA FISH signal in planaria.
    Article Snippet: .. Load desired wells of a 96well skirted PCR plate with an appropriate collection buffer (e.g., SMART-Seq mRNA kit (Takara Bio) lysis buffer). ..

    Article Title: Constitutive interferon epsilon expression shapes antiviral epithelial states in the female reproductive tract and intestine.
    Article Snippet: .. Geno typing of the resulting pups was carried out by extracting genomic DNA from toe biopsies using a lysis buffer containing 50 mM Tris-base (pH 8.0), 50 mM KCl, 2.5 mM EDTA, 0.45% IGEPAL CA-630 (NP40), 0.45% Tween-20, and 20 mg/mL proteinase K, followed by incubation at 94°C for 10 min. PCR amplification was performed with primers flanking the gRNA target sites (forward: TCCCAGAACTGGAGTGGT; reverse: AA GAGCCAACAGGGGATTT) using TITANIUM Taq DNA Polymerase (Takara). ..

    Lysis:

    Article Title: Protocol for purifying and sequencing cell subpopulations based on RNA FISH signal in planaria.
    Article Snippet: .. Load desired wells of a 96well skirted PCR plate with an appropriate collection buffer (e.g., SMART-Seq mRNA kit (Takara Bio) lysis buffer). ..

    Article Title: Constitutive interferon epsilon expression shapes antiviral epithelial states in the female reproductive tract and intestine.
    Article Snippet: .. Geno typing of the resulting pups was carried out by extracting genomic DNA from toe biopsies using a lysis buffer containing 50 mM Tris-base (pH 8.0), 50 mM KCl, 2.5 mM EDTA, 0.45% IGEPAL CA-630 (NP40), 0.45% Tween-20, and 20 mg/mL proteinase K, followed by incubation at 94°C for 10 min. PCR amplification was performed with primers flanking the gRNA target sites (forward: TCCCAGAACTGGAGTGGT; reverse: AA GAGCCAACAGGGGATTT) using TITANIUM Taq DNA Polymerase (Takara). ..

    Article Title: Heterogeneous distribution of avian malaria parasites across mosquito species: disproportionate involvement of Culex restuans in the Midwestern United States.
    Article Snippet: .. Briefly, pooled mosquitoes were homogenized in 960 μL Takara LBP lysis buffer (NucleoSpin 96 DNA RapidLyse Kit, Takara Bio, Japan) supplemented with 40 μL 1 M DTT and 20 μL liquid Proteinase K. Homogenization was performed using a TissueLyser system (Qiagen, Hilden, Germany) at 30 Hz for two 5-min cycles, with the rack orientation changed between cycles, using five stainless steel beads per tube. .. Following centrifugation at 6,000 rpm for 10 min, lysates were incubated at 56°C for 10 min. A total of 200 μL of the clarified supernatant was subjected to automated nucleic acid purification using the MagMAXTM Viral/Pathogen Ultra Nucleic Acid Isolation Kit (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) on a KingFisherTM Flex Magnetic Particle Processor (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.

    Article Title: Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production
    Article Snippet: .. For bulk-seq, 500 astrocytes/sample were collected in 4μl cell lysis buffer [0.2% Triton X-100 and RNAse inhibitor (2 U/μl) (CloneTech)]. ..

    Article Title: Oxygen gradients reshape cross-feeding through emergent spatial organization of gut commensal bacteria
    Article Snippet: .. Cell pellets were washed and resuspended in 100 μ l of PBS, and 1 μ l of a 1:20 dilution was dispensed in a 48-well plate pre-filled with 0.26 μ l PBS, 0.26 μ l lysis buffer (Takara), 0.1 U lysozyme (50 U/ μ l; Epicentre), 0.03 μ l RNase inhibitor (100 U/ μ l; Takara), and 1.95 μ l H 2 O. .. The plate was then sealed with an aluminum-based microfilm (Bio-Rad seal F), sonicated for 20 seconds (Sonorex Digitec DT 52, Bendelin), and spun down.

    Article Title: RegVelo: Gene-regulatory-informed dynamics of single cells.
    Article Snippet: To generate full-transcript-length, deep scRNA-Seq data, we prepared the Smart-seq3 libraries following the protocol.99 During FACS, cells were sorted into individual wells containing 3-μl lysis buffer dispensed by the MANTIS liquid handler (FORMULATRIX). .. The lysis buffer consisted of 5% polyethylene glycol 8000 (Sigma), 0.1% Triton X-100 (Sigma), 0.5 U/μl RNAse inhibitor (TaKaRa), 0.5 μM OligoT30VN (IDT), 0.5 mM/each dNTPs (Thermo Fisher) and nuclease-free water (Thermo Fisher). ..

    Article Title: Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stress
    Article Snippet: Colony size was measured by ImageJ (NIH) on images taken by a BX41 phase contrast microscope (Olympus) using an Infinity2 microscopy camera (Lumenera). .. To compare the transcriptome of HSPCs based on their relative use of IRES/Cap, 100 SLAM-LKS, GMP, or MEP per well were sorted on the basis of IRES/Cap from the Translator mouse into 2.6 μl of Lysis Buffer (Takara Bio USA Inc.) followed by snap freezing at −80°C in preparation for cDNA synthesis using the SMART-Seq v4 assay. ..

    Article Title: Whole-protein screening and multi-modal profiling of antigen-specific CD4 + T cells at single-cell resolution
    Article Snippet: .. The cells were single-cell sorted into 96-well plates containing 12 uL of 1x lysis buffer (Takara, 635013) with RNAse inhibitor (Promega, N2515). ..

    Incubation:

    Article Title: Constitutive interferon epsilon expression shapes antiviral epithelial states in the female reproductive tract and intestine.
    Article Snippet: .. Geno typing of the resulting pups was carried out by extracting genomic DNA from toe biopsies using a lysis buffer containing 50 mM Tris-base (pH 8.0), 50 mM KCl, 2.5 mM EDTA, 0.45% IGEPAL CA-630 (NP40), 0.45% Tween-20, and 20 mg/mL proteinase K, followed by incubation at 94°C for 10 min. PCR amplification was performed with primers flanking the gRNA target sites (forward: TCCCAGAACTGGAGTGGT; reverse: AA GAGCCAACAGGGGATTT) using TITANIUM Taq DNA Polymerase (Takara). ..

    Amplification:

    Article Title: Constitutive interferon epsilon expression shapes antiviral epithelial states in the female reproductive tract and intestine.
    Article Snippet: .. Geno typing of the resulting pups was carried out by extracting genomic DNA from toe biopsies using a lysis buffer containing 50 mM Tris-base (pH 8.0), 50 mM KCl, 2.5 mM EDTA, 0.45% IGEPAL CA-630 (NP40), 0.45% Tween-20, and 20 mg/mL proteinase K, followed by incubation at 94°C for 10 min. PCR amplification was performed with primers flanking the gRNA target sites (forward: TCCCAGAACTGGAGTGGT; reverse: AA GAGCCAACAGGGGATTT) using TITANIUM Taq DNA Polymerase (Takara). ..

    Homogenization:

    Article Title: Heterogeneous distribution of avian malaria parasites across mosquito species: disproportionate involvement of Culex restuans in the Midwestern United States.
    Article Snippet: .. Briefly, pooled mosquitoes were homogenized in 960 μL Takara LBP lysis buffer (NucleoSpin 96 DNA RapidLyse Kit, Takara Bio, Japan) supplemented with 40 μL 1 M DTT and 20 μL liquid Proteinase K. Homogenization was performed using a TissueLyser system (Qiagen, Hilden, Germany) at 30 Hz for two 5-min cycles, with the rack orientation changed between cycles, using five stainless steel beads per tube. .. Following centrifugation at 6,000 rpm for 10 min, lysates were incubated at 56°C for 10 min. A total of 200 μL of the clarified supernatant was subjected to automated nucleic acid purification using the MagMAXTM Viral/Pathogen Ultra Nucleic Acid Isolation Kit (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) on a KingFisherTM Flex Magnetic Particle Processor (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.

    cDNA Synthesis:

    Article Title: Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stress
    Article Snippet: Colony size was measured by ImageJ (NIH) on images taken by a BX41 phase contrast microscope (Olympus) using an Infinity2 microscopy camera (Lumenera). .. To compare the transcriptome of HSPCs based on their relative use of IRES/Cap, 100 SLAM-LKS, GMP, or MEP per well were sorted on the basis of IRES/Cap from the Translator mouse into 2.6 μl of Lysis Buffer (Takara Bio USA Inc.) followed by snap freezing at −80°C in preparation for cDNA synthesis using the SMART-Seq v4 assay. ..

    Single Cell:

    Article Title: Whole-protein screening and multi-modal profiling of antigen-specific CD4 + T cells at single-cell resolution
    Article Snippet: .. The cells were single-cell sorted into 96-well plates containing 12 uL of 1x lysis buffer (Takara, 635013) with RNAse inhibitor (Promega, N2515). ..



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