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flp intm t rextm 293 cell line  (Thermo Fisher)


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    Structured Review

    Thermo Fisher flp intm t rextm 293 cell line
    Flp Intm T Rextm 293 Cell Line, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single-cell+spatial+transcriptomics+data/Tetracycline/us11965191-832-150-183
    Average 99 stars, based on 1 article reviews
    flp intm t rextm 293 cell line - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1
    Article Snippet: COS-7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (Nissui) containing 10% FBS, 4 mM L-glutamine, 100 U/ml penicillin, and 100 U/ml streptomycin (Meiji Seika Pharma) at 37 °C in 5% CO 2 . .. To establish the human c-Src-inducible HCT116 cell line, HCT116-c-Src, the pcDNA4/TO/c-Src plasmid ( ) for the inducible expression of c-Src was transfected into HCT116/TR cells , which express the tetracycline repressor, by lipofection using Lipofectamine 2000 reagent (Invitrogen). ..

    Expressing:

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1
    Article Snippet: COS-7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (Nissui) containing 10% FBS, 4 mM L-glutamine, 100 U/ml penicillin, and 100 U/ml streptomycin (Meiji Seika Pharma) at 37 °C in 5% CO 2 . .. To establish the human c-Src-inducible HCT116 cell line, HCT116-c-Src, the pcDNA4/TO/c-Src plasmid ( ) for the inducible expression of c-Src was transfected into HCT116/TR cells , which express the tetracycline repressor, by lipofection using Lipofectamine 2000 reagent (Invitrogen). ..

    Article Title: Human DEAH-box helicase 8 regulates HSF1-mediated stress response and cancer-associated pre-mRNA splicing in tumour cells
    Article Snippet: .. Stable tetracycline repressor expressing human colon cancer HCT116 T-REx cell lines were generated using the pLenti3.3/TR vector (Thermo Fisher Scientific). .. QuickChange Lightning site-directed mutagenesis (Agilent Technologies) was used to generate DHX8 mutants.

    Transfection:

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1
    Article Snippet: COS-7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (Nissui) containing 10% FBS, 4 mM L-glutamine, 100 U/ml penicillin, and 100 U/ml streptomycin (Meiji Seika Pharma) at 37 °C in 5% CO 2 . .. To establish the human c-Src-inducible HCT116 cell line, HCT116-c-Src, the pcDNA4/TO/c-Src plasmid ( ) for the inducible expression of c-Src was transfected into HCT116/TR cells , which express the tetracycline repressor, by lipofection using Lipofectamine 2000 reagent (Invitrogen). ..

    other:

    Article Title: Targeting CBL ubiquitin ligase activation to downregulate tyrosine kinase signalling
    Article Snippet: 32D cells were maintained in RPMI 1640 (Wisent) with 10% heat inactivated tetracycline-free FBS (Wisent) and supplemented with 5 ng/mL IL-3 (Peprotech (Thermo Fisher Scientific) # 213-13) maintained in fresh media every 2-3 days.

    Generated:

    Article Title: Human DEAH-box helicase 8 regulates HSF1-mediated stress response and cancer-associated pre-mRNA splicing in tumour cells
    Article Snippet: .. Stable tetracycline repressor expressing human colon cancer HCT116 T-REx cell lines were generated using the pLenti3.3/TR vector (Thermo Fisher Scientific). .. QuickChange Lightning site-directed mutagenesis (Agilent Technologies) was used to generate DHX8 mutants.

    Transduction:

    Article Title: Nup358 Sustains Intestinal Epithelial Homeostasis by Preventing Dvl1 Condensate Formation to Restrain Wnt Signaling
    Article Snippet: .. 293T cells were transduced with pTRIPZ tetracycline-inducible short hairpin RNA (shRNA) lentiviruses (control RHS4743 or Nup358 targeting 200761584, 200765221, and 200766435 from Dharmacon), followed by 12 days selection with Puromycin (1.25 μg/ml; Gibco). .. Expression of shRNA was induced with doxycycline (200 ng/ml; Clontech).

    shRNA:

    Article Title: Nup358 Sustains Intestinal Epithelial Homeostasis by Preventing Dvl1 Condensate Formation to Restrain Wnt Signaling
    Article Snippet: .. 293T cells were transduced with pTRIPZ tetracycline-inducible short hairpin RNA (shRNA) lentiviruses (control RHS4743 or Nup358 targeting 200761584, 200765221, and 200766435 from Dharmacon), followed by 12 days selection with Puromycin (1.25 μg/ml; Gibco). .. Expression of shRNA was induced with doxycycline (200 ng/ml; Clontech).

    Control:

    Article Title: Nup358 Sustains Intestinal Epithelial Homeostasis by Preventing Dvl1 Condensate Formation to Restrain Wnt Signaling
    Article Snippet: .. 293T cells were transduced with pTRIPZ tetracycline-inducible short hairpin RNA (shRNA) lentiviruses (control RHS4743 or Nup358 targeting 200761584, 200765221, and 200766435 from Dharmacon), followed by 12 days selection with Puromycin (1.25 μg/ml; Gibco). .. Expression of shRNA was induced with doxycycline (200 ng/ml; Clontech).

    Selection:

    Article Title: Nup358 Sustains Intestinal Epithelial Homeostasis by Preventing Dvl1 Condensate Formation to Restrain Wnt Signaling
    Article Snippet: .. 293T cells were transduced with pTRIPZ tetracycline-inducible short hairpin RNA (shRNA) lentiviruses (control RHS4743 or Nup358 targeting 200761584, 200765221, and 200766435 from Dharmacon), followed by 12 days selection with Puromycin (1.25 μg/ml; Gibco). .. Expression of shRNA was induced with doxycycline (200 ng/ml; Clontech).

    Activation Assay:

    Article Title: Extended perfused culture of cm-scale endocrine pancreatic tissues created through sacrificial embedded printing into alginate
    Article Snippet: Passage numbers were kept below 40 and we used cell suspensions with a viability of at least 85%, measured using trypan blue staining (MilliporeSigma, T8154) and automated cell counting (TC20, Bio-Rad, 1450102). .. The conditionally immortalized βTC-tet cell line which undergoes growth arrest upon activation of the tetracycline operator (ATCC, CRL-3237, received at unknown passage x, master bank at passage x+3, and working banks at passage x+7) was cultured in Dulbecco’s modified Eagle’s medium supplemented with 2.5% FBS, 15% heat-inactivated horse serum (Thermo Fisher Scientific, 26050088), 100 U/mL penicillin/streptomycin, and 2 mM L-glutamine. ..

    Cell Culture:

    Article Title: Extended perfused culture of cm-scale endocrine pancreatic tissues created through sacrificial embedded printing into alginate
    Article Snippet: Passage numbers were kept below 40 and we used cell suspensions with a viability of at least 85%, measured using trypan blue staining (MilliporeSigma, T8154) and automated cell counting (TC20, Bio-Rad, 1450102). .. The conditionally immortalized βTC-tet cell line which undergoes growth arrest upon activation of the tetracycline operator (ATCC, CRL-3237, received at unknown passage x, master bank at passage x+3, and working banks at passage x+7) was cultured in Dulbecco’s modified Eagle’s medium supplemented with 2.5% FBS, 15% heat-inactivated horse serum (Thermo Fisher Scientific, 26050088), 100 U/mL penicillin/streptomycin, and 2 mM L-glutamine. ..

    Article Title: Centriolar satellites regulate CEP350 mRNA localization and centrosome amplification
    Article Snippet: .. MDA-MB-231 (University of Colorado Cancer Center Tissue Culture Core), RPE-1-Tet-PLK4, Centrin2:GFP , and HEK293T cells were cultured in DMEM/F12 + L-glutamine (Cytiva or Life Technologies), 10% tetracycline-free FBS (Peak Serum), with 1% penicillin and streptomycin (Life Technologies) at 37°C and 5% CO 2 . .. MCF10A cells were cultured in Ham’s F/12 + L-glutamine (Corning), 5% horse serum (16050122; Thermo Fisher Scientific), Human EGF Recombinant Protein 20 ng/ml (PHG0311; Thermo Fisher Scientific), hydrocortisone 500 ng/ml (H0888; Sigma-Aldrich), cholera toxin 100 ng/ml (C8052; Sigma-Aldrich), insulin 10 μg/ml (I1882; Sigma-Aldrich), with 1% penicillin and streptomycin (Life Technologies) at 37°C and 5% CO 2 .

    Modification:

    Article Title: Extended perfused culture of cm-scale endocrine pancreatic tissues created through sacrificial embedded printing into alginate
    Article Snippet: Passage numbers were kept below 40 and we used cell suspensions with a viability of at least 85%, measured using trypan blue staining (MilliporeSigma, T8154) and automated cell counting (TC20, Bio-Rad, 1450102). .. The conditionally immortalized βTC-tet cell line which undergoes growth arrest upon activation of the tetracycline operator (ATCC, CRL-3237, received at unknown passage x, master bank at passage x+3, and working banks at passage x+7) was cultured in Dulbecco’s modified Eagle’s medium supplemented with 2.5% FBS, 15% heat-inactivated horse serum (Thermo Fisher Scientific, 26050088), 100 U/mL penicillin/streptomycin, and 2 mM L-glutamine. ..

    Multiple Displacement Amplification:

    Article Title: Centriolar satellites regulate CEP350 mRNA localization and centrosome amplification
    Article Snippet: .. MDA-MB-231 (University of Colorado Cancer Center Tissue Culture Core), RPE-1-Tet-PLK4, Centrin2:GFP , and HEK293T cells were cultured in DMEM/F12 + L-glutamine (Cytiva or Life Technologies), 10% tetracycline-free FBS (Peak Serum), with 1% penicillin and streptomycin (Life Technologies) at 37°C and 5% CO 2 . .. MCF10A cells were cultured in Ham’s F/12 + L-glutamine (Corning), 5% horse serum (16050122; Thermo Fisher Scientific), Human EGF Recombinant Protein 20 ng/ml (PHG0311; Thermo Fisher Scientific), hydrocortisone 500 ng/ml (H0888; Sigma-Aldrich), cholera toxin 100 ng/ml (C8052; Sigma-Aldrich), insulin 10 μg/ml (I1882; Sigma-Aldrich), with 1% penicillin and streptomycin (Life Technologies) at 37°C and 5% CO 2 .



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    (a) Simplified cross-section of the human epidermis, highlighting squamous cells, melanocytes and basal cells. Coloured regions represent cSCC (green), which originates from squamous cells, melanoma (orange), which originates from melanocytes, and BCC (blue), which originates from basal cells. Two orange melanocytes are shown in the dermal region as occurs in invasive melanoma; other cells in the lower dermis layer are not depicted. (b) Overview of sample design and technologies used to generate data for this project. ROI - region of interest; FOV - field of view; S - cSCC; B - BCC; M - melanoma; HC - healthy (cancer patient); HNC - healthy (non-cancer patient donor). Technologies included are single cell RNA sequencing for fresh samples, single nuclei sequencing for formalin-fixed samples, Visium, Xenium, CosMX, GeoMX DSP for whole transcriptome, GeoMX DSP for proteins, Polaris, RNAscope, the proximal ligation assay, spatial glycomics and CODEX.

    Journal: bioRxiv

    Article Title: Integrating 12 Spatial and Single Cell Technologies to Characterise Tumour Neighbourhoods and Cellular Interactions in three Skin Cancer Types

    doi: 10.1101/2025.07.25.666708

    Figure Lengend Snippet: (a) Simplified cross-section of the human epidermis, highlighting squamous cells, melanocytes and basal cells. Coloured regions represent cSCC (green), which originates from squamous cells, melanoma (orange), which originates from melanocytes, and BCC (blue), which originates from basal cells. Two orange melanocytes are shown in the dermal region as occurs in invasive melanoma; other cells in the lower dermis layer are not depicted. (b) Overview of sample design and technologies used to generate data for this project. ROI - region of interest; FOV - field of view; S - cSCC; B - BCC; M - melanoma; HC - healthy (cancer patient); HNC - healthy (non-cancer patient donor). Technologies included are single cell RNA sequencing for fresh samples, single nuclei sequencing for formalin-fixed samples, Visium, Xenium, CosMX, GeoMX DSP for whole transcriptome, GeoMX DSP for proteins, Polaris, RNAscope, the proximal ligation assay, spatial glycomics and CODEX.

    Article Snippet: Cells expressing the two genes are visualized on single-cell level resolution spatial data from STOmics and Curio-Seeker (Takara Bio, USA) melanoma samples and appear to be in spatial proximity ( ).

    Techniques: RNA Sequencing, Sequencing, RNAscope, Ligation

    (a) Gene specificity score (GSS) and association of spatial spots with skin cancer heritability. GSS score for each gene in a spot/cell represents the enrichment of the gene as a top rank most abundant gene in the spot/cell and its neighbour spots/cells in an anatomical region, a spatial domain, or a cell type. The p-value shows the spatial heritability enrichment significance of a spot with a trait based on SNPs mapped to the genes with high GSS scores (one-sided Z-test for stratified coefficient different to 0). The p-value is more significant if the SNPs that are mapped to the high GSS genes explain a higher proportion of heritability for the trait. (b) Cell types with the highest enrichment of heritability explained by SNPs tagged to GSS genes of cells in a cell type. The white asterisks indicate the most enriched cell-type for heritability of cutaneous melanoma, cSCC and BCC traits. (c) gsMAP significance spatial heritability enrichment is shown at single-cell resolution across the tissue (upper tissue plots) or per annotated skin regions (lower violin plots) from the cosMx data of the sample mel48974. (d) LR pairs with significant association with SNP heritability explained by the corresponding cell types. The rectangles show cases where both L and R genes had PCC >0.3 between GSS of the gene and the gsMAP P-values (the significance level for the LD stratified coefficients for the spot bigger than 0). The results suggest which LR pairs are related with the heritability of a cell type pairs. (e) GSS of two LR pairs showing specificity of the L and R genes to tissue regions at the immune-rich dermal layers and the epidermis of the skin. (f) Manhattan plot showing top significant GWAS SNPs co-localizing with genes in melanocytes (red) and T cells (blue) that had the highest Pearson correlation between GSS and the gsMAP trait association P-value or associated with SNPs with genome-wide significance. The Y-axis shows the -log(P-value) from GWAS analysis.

    Journal: bioRxiv

    Article Title: Integrating 12 Spatial and Single Cell Technologies to Characterise Tumour Neighbourhoods and Cellular Interactions in three Skin Cancer Types

    doi: 10.1101/2025.07.25.666708

    Figure Lengend Snippet: (a) Gene specificity score (GSS) and association of spatial spots with skin cancer heritability. GSS score for each gene in a spot/cell represents the enrichment of the gene as a top rank most abundant gene in the spot/cell and its neighbour spots/cells in an anatomical region, a spatial domain, or a cell type. The p-value shows the spatial heritability enrichment significance of a spot with a trait based on SNPs mapped to the genes with high GSS scores (one-sided Z-test for stratified coefficient different to 0). The p-value is more significant if the SNPs that are mapped to the high GSS genes explain a higher proportion of heritability for the trait. (b) Cell types with the highest enrichment of heritability explained by SNPs tagged to GSS genes of cells in a cell type. The white asterisks indicate the most enriched cell-type for heritability of cutaneous melanoma, cSCC and BCC traits. (c) gsMAP significance spatial heritability enrichment is shown at single-cell resolution across the tissue (upper tissue plots) or per annotated skin regions (lower violin plots) from the cosMx data of the sample mel48974. (d) LR pairs with significant association with SNP heritability explained by the corresponding cell types. The rectangles show cases where both L and R genes had PCC >0.3 between GSS of the gene and the gsMAP P-values (the significance level for the LD stratified coefficients for the spot bigger than 0). The results suggest which LR pairs are related with the heritability of a cell type pairs. (e) GSS of two LR pairs showing specificity of the L and R genes to tissue regions at the immune-rich dermal layers and the epidermis of the skin. (f) Manhattan plot showing top significant GWAS SNPs co-localizing with genes in melanocytes (red) and T cells (blue) that had the highest Pearson correlation between GSS and the gsMAP trait association P-value or associated with SNPs with genome-wide significance. The Y-axis shows the -log(P-value) from GWAS analysis.

    Article Snippet: Cells expressing the two genes are visualized on single-cell level resolution spatial data from STOmics and Curio-Seeker (Takara Bio, USA) melanoma samples and appear to be in spatial proximity ( ).

    Techniques: Genome Wide

    Journal: Cell

    Article Title: Spatiotemporal analysis of human intestinal development at single-cell resolution

    doi: 10.1016/j.cell.2020.12.016

    Figure Lengend Snippet:

    Article Snippet: STAR-FINDer Single Cell and Spatial Transcriptomics Data Portal , This study , https://simmonslab.shinyapps.io/FetalAtlasDataPortal.

    Techniques: Conjugation Assay, Recombinant, Saline, Modification, Plasmid Preparation, Gene Expression, RNAscope, Generated, Software