rabbit anti mouse cd44 primary antibody (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology rabbit anti mouse cd44 primary antibody

Distribution of <t>CD44</t> + cells within intestinal epithelium. a , b Immunohistochemical (IHC) staining for Lgr5 + ISCs ( black dotted lines ) in vivo. c , d IHC staining for CD44 + cells ( black dotted lines ) in vivo. a , c Magnification ×400. Bars 50 μm. b , d Magnification ×1000. Bars 20 μm. e–n Immunocytochemical (ICC) staining for CD44 + cells in vitro. e , j Differential interference contrast (DIC) imaging. f , k Propidium iodide (PI) staining for nuclei. g , l Fluorescein isothiocyanate (FITC) for CD44 + cells ( white arrowheads crypt cells strongly positive for CD44). h , m Overlay of PI image and FITC image. i , n Overlay of FITC image and DIC image. e–i Magnification ×200. Bars 200 μm. j–n Magnification ×630. Bars 100 μm

Rabbit Anti Mouse Cd44 Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2006 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/single-cell+spatial+transcriptomics+data/rabbit+anti-mouse+IgG-HRP/pmc04846698-61-6-11
Average 96 stars, based on 2006 article reviews

rabbit anti mouse cd44 primary antibody - by Bioz Stars, 2026-09

96/100 stars

Images

1) Product Images from "Mensenchymal stem cells can delay radiation-induced crypt death: impact on intestinal CD44 + fragments"

Article Title: Mensenchymal stem cells can delay radiation-induced crypt death: impact on intestinal CD44 + fragments

Journal: Cell and Tissue Research

doi: 10.1007/s00441-015-2313-6

Distribution of CD44 + cells within intestinal epithelium. a , b Immunohistochemical (IHC) staining for Lgr5 + ISCs ( black dotted lines ) in vivo. c , d IHC staining for CD44 + cells ( black dotted lines ) in vivo. a , c Magnification ×400. Bars 50 μm. b , d Magnification ×1000. Bars 20 μm. e–n Immunocytochemical (ICC) staining for CD44 + cells in vitro. e , j Differential interference contrast (DIC) imaging. f , k Propidium iodide (PI) staining for nuclei. g , l Fluorescein isothiocyanate (FITC) for CD44 + cells ( white arrowheads crypt cells strongly positive for CD44). h , m Overlay of PI image and FITC image. i , n Overlay of FITC image and DIC image. e–i Magnification ×200. Bars 200 μm. j–n Magnification ×630. Bars 100 μm

Figure Legend Snippet: Distribution of CD44 + cells within intestinal epithelium. a , b Immunohistochemical (IHC) staining for Lgr5 + ISCs ( black dotted lines ) in vivo. c , d IHC staining for CD44 + cells ( black dotted lines ) in vivo. a , c Magnification ×400. Bars 50 μm. b , d Magnification ×1000. Bars 20 μm. e–n Immunocytochemical (ICC) staining for CD44 + cells in vitro. e , j Differential interference contrast (DIC) imaging. f , k Propidium iodide (PI) staining for nuclei. g , l Fluorescein isothiocyanate (FITC) for CD44 + cells ( white arrowheads crypt cells strongly positive for CD44). h , m Overlay of PI image and FITC image. i , n Overlay of FITC image and DIC image. e–i Magnification ×200. Bars 200 μm. j–n Magnification ×630. Bars 100 μm

Techniques Used: Immunohistochemical staining, Immunohistochemistry, In Vivo, Staining, In Vitro, Imaging

Identification of CD44 + cells. a–a’’’’’ Fluorescence-activated cell sorting (FACS) analysis for cellular phenotype. a Isotype control, IgG2a-phycoerythrin (PE). a’ CD31-PE. a’’ CD34-PE. a’’’ Isotype control, IgG2b-allophycocyanin (APC). a’’’’ CD44-APC ( lo low-positive for CD44, hi high-positive for CD44). a’’’’’ CD45-APC. b Development of CD44 + ISC in 3D-culture system. Numbers represent days. Top Magnification ×400. Bar 50 μm. Bottom Magnification ×200. Bars 100 μm. c–c’’ Colony-forming efficacies of CD44 - cells and CD44 + cells. c CD44 - cells in 3D-culture system for 14 days. c’ CD44 + cells in 3D-culture system for 14 days. c , c’ Magnification ×40. Bars 500 μm. c’’ Comparision of colony-forming efficacy per 100 sorted cells seeded in one well of a 96-well plate. CD44 - group in 48 wells; CD44 + group in 48 wells. Data represent means ± SD of 48 independent measurements ( n = 48). Bars SD values. Paired t -test was used for data analysis. * P ≤ 0.05 represents high significance (CD44 + group versus CD44 - group). All experimental procedures were repeated twice. d–d’’’’ Transmission electron microscope imaging of CD44 + ISC differentiation. CD44 + ISC were cultured in the 3D-system for 6 days and formed a cystic structure. d Cystic structure of a single CD44 + ISC-derived organoid at 6 days. Boxed areas are shown at higher magnification in d’–d’’’’ ( Lu lumen). Magnification ×400. Bar 50 μm. d’ Absorptive cell ( Ab ). d’’ Endocrine cell ( En ). d’’’ Goblet cell ( Go ). d’’’’ Paneth cell ( Pa ). Black arrowhead in d’ indicates brush border. Black arrowheads in d’’–d’’’’ indicates granules. d’–d’’’’ Magnification ×1500. Bars 5 μm

Figure Legend Snippet: Identification of CD44 + cells. a–a’’’’’ Fluorescence-activated cell sorting (FACS) analysis for cellular phenotype. a Isotype control, IgG2a-phycoerythrin (PE). a’ CD31-PE. a’’ CD34-PE. a’’’ Isotype control, IgG2b-allophycocyanin (APC). a’’’’ CD44-APC ( lo low-positive for CD44, hi high-positive for CD44). a’’’’’ CD45-APC. b Development of CD44 + ISC in 3D-culture system. Numbers represent days. Top Magnification ×400. Bar 50 μm. Bottom Magnification ×200. Bars 100 μm. c–c’’ Colony-forming efficacies of CD44 - cells and CD44 + cells. c CD44 - cells in 3D-culture system for 14 days. c’ CD44 + cells in 3D-culture system for 14 days. c , c’ Magnification ×40. Bars 500 μm. c’’ Comparision of colony-forming efficacy per 100 sorted cells seeded in one well of a 96-well plate. CD44 - group in 48 wells; CD44 + group in 48 wells. Data represent means ± SD of 48 independent measurements ( n = 48). Bars SD values. Paired t -test was used for data analysis. * P ≤ 0.05 represents high significance (CD44 + group versus CD44 - group). All experimental procedures were repeated twice. d–d’’’’ Transmission electron microscope imaging of CD44 + ISC differentiation. CD44 + ISC were cultured in the 3D-system for 6 days and formed a cystic structure. d Cystic structure of a single CD44 + ISC-derived organoid at 6 days. Boxed areas are shown at higher magnification in d’–d’’’’ ( Lu lumen). Magnification ×400. Bar 50 μm. d’ Absorptive cell ( Ab ). d’’ Endocrine cell ( En ). d’’’ Goblet cell ( Go ). d’’’’ Paneth cell ( Pa ). Black arrowhead in d’ indicates brush border. Black arrowheads in d’’–d’’’’ indicates granules. d’–d’’’’ Magnification ×1500. Bars 5 μm

Techniques Used: Fluorescence, FACS, Control, Transmission Assay, Microscopy, Imaging, Cell Culture, Derivative Assay

CD44 + ISCs resemble CBC stem cells. a–a’’ Strategy for sorting CD44 - , CD44 low+ and CD44 hi+ subpopulations by using the FACS technique ( R1 determining the cell-zone, R2 determining viable cells, PI propidium iodide, APC allophycocyanin). b Semi-quantitative reverse transcription (RT) followed by the polymerase chain reaction (PCR) for ISC-related gene-expression in sorted cells. Fold expression values were normalized to the CD44 - group. Data represent means ± SD of six independent measurements ( n = 6). Bars indicate value of SD. The paired t -test was used for data analysis. * P ≤ 0.05 represents high significance (CD44 hi+ group versus CD44 low+ group); $ P ≤ 0.05 represents low significance (CD44 hi+ group versus CD44 low+ group); ns represents no statistic differences between the CD44 hi+ group and CD44 low+ group. P -values for Lgr5 , Bmi1 , Hopx , mTERT , Ascl2 , Smoc2 , Lrig1 , Rnf43 and Prominin-1 are respectively 0.016, 0.036, 0.010, 0.844, 0.007, 0.001, 0.041, 0.005 and 0.859. c , c’ Colony-forming efficacy of CD44 low+ and CD44 hi+ cells in 3D-culture system for 14 days. c CD44 low+ group. c’ CD44 hi+ group. Magnification ×40. Bars 500 μm

Figure Legend Snippet: CD44 + ISCs resemble CBC stem cells. a–a’’ Strategy for sorting CD44 - , CD44 low+ and CD44 hi+ subpopulations by using the FACS technique ( R1 determining the cell-zone, R2 determining viable cells, PI propidium iodide, APC allophycocyanin). b Semi-quantitative reverse transcription (RT) followed by the polymerase chain reaction (PCR) for ISC-related gene-expression in sorted cells. Fold expression values were normalized to the CD44 - group. Data represent means ± SD of six independent measurements ( n = 6). Bars indicate value of SD. The paired t -test was used for data analysis. * P ≤ 0.05 represents high significance (CD44 hi+ group versus CD44 low+ group); $ P ≤ 0.05 represents low significance (CD44 hi+ group versus CD44 low+ group); ns represents no statistic differences between the CD44 hi+ group and CD44 low+ group. P -values for Lgr5 , Bmi1 , Hopx , mTERT , Ascl2 , Smoc2 , Lrig1 , Rnf43 and Prominin-1 are respectively 0.016, 0.036, 0.010, 0.844, 0.007, 0.001, 0.041, 0.005 and 0.859. c , c’ Colony-forming efficacy of CD44 low+ and CD44 hi+ cells in 3D-culture system for 14 days. c CD44 low+ group. c’ CD44 hi+ group. Magnification ×40. Bars 500 μm

Techniques Used: Reverse Transcription, Polymerase Chain Reaction, Gene Expression, Expressing

Epithelial homeostasis in CD44 + ISC-derived organoid. a , b TUNEL staining for apoptotic cells in normal epithelium. c , d IHC staining of Ki67 for proliferative cells within normal epithelium. a , c Magnification ×200. Bars 100 μm. b , d Magnification ×400. Bars 50 μm. e–j Epithelial homeostasis in vitro. e–g TUNEL staining for apoptotic cells in CD44 + ISC-derived organoid. e DAPI staining ( blue ) for nuclei. f dUTP-FITC ( green ) for apoptotic cells. g DAPI image merged with dUTP-FITC image. Magnification ×100. Bars 200 μm. h–j ICC staining for proliferative cells in CD44 + ISC-derived organoid. h DAPI staining for nuclei. i Ki67-FITC for proliferative cells. j DAPI image merged with Ki67-FITC image. Magnification ×200. Bar 100 μm. k Representation of an organoid

Figure Legend Snippet: Epithelial homeostasis in CD44 + ISC-derived organoid. a , b TUNEL staining for apoptotic cells in normal epithelium. c , d IHC staining of Ki67 for proliferative cells within normal epithelium. a , c Magnification ×200. Bars 100 μm. b , d Magnification ×400. Bars 50 μm. e–j Epithelial homeostasis in vitro. e–g TUNEL staining for apoptotic cells in CD44 + ISC-derived organoid. e DAPI staining ( blue ) for nuclei. f dUTP-FITC ( green ) for apoptotic cells. g DAPI image merged with dUTP-FITC image. Magnification ×100. Bars 200 μm. h–j ICC staining for proliferative cells in CD44 + ISC-derived organoid. h DAPI staining for nuclei. i Ki67-FITC for proliferative cells. j DAPI image merged with Ki67-FITC image. Magnification ×200. Bar 100 μm. k Representation of an organoid

Techniques Used: Derivative Assay, TUNEL Assay, Staining, Immunohistochemistry, In Vitro

Control:

Article Title: CMAH-Targeted Knock-In of Inducible Heme Oxygenase-1 and Constitutive CD47 in GGTA1-Knockout Pigs for Xenotransplantation.
Article Snippet: Protein expression was visualized using an ECL etection reagent (GE Healthcare, Buckinghamshire, UK). .. The ollowing primary and secondary antibodies were used: mouse onoclonal anti-HO1 (Abcam) with anti-mouse IgG κ BP-HRP Santa Cruz Biotechnology, Dallas, TX, USA); rabbit monoclonal nti-CD47 (Abcam) with mouse anti-rabbit IgG-HRP (Santa Cruz iotechnology); and rabbit monoclonal anti-Vinculin (Abcam) ith mouse anti-rabbit IgG-HRP (Santa Cruz Biotechnology) as loading control. .. .11 Flow Cytometry eripheral blood mononuclear cells (PBMCs) were isolated from eparinized blood using density gradient centrifugation with icoll-Paque (GE Healthcare, Chicago, IL, USA), according to the anufacturer’s instructions.

Immunoprecipitation:

Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria.
Article Snippet: Recombinant TGFβ and TGFβ type I receptor inhibitor SB431542 were purchased from Sigma-Aldrich (T7039 and S4696). .. The following antibodies were used: PC7-conjugated anti-human CD235a (GPA–PC7, 1:50) supplied by Beckman Coulter, clone 11E4B-7-6, A71564; PE-conjugated anti-Band3 (Band 3–PE, 1:100) supplied by IBGRL, clone BRIC 6, 9439PE; APC-conjugated anti-CD49d (integrin α4–APC, 1:20) supplied by Beckman Coulter, clone HP2/1, B01682; anti-human GATA1 (GATA1, 1:10) supplied by Santa Cruz Biotechnology, clone N1, sc-266X; anti-influenza haemagglutinin epitope tag HA.11 (SMAD3–HA, 1:10) supplied by BioLegend, clone 16B12, MMS-101P; anti-human cyclin D3 (cyclin D3, 1:250) supplied by Santa Cruz Biotechnology, clone D-7, sc-6283; anti-human SMAD2 (SMAD2, 1:500) supplied by Santa Cruz Biotechnology, clone S-20, sc-6200; anti-β-actin (β-actin, 1:1,000) supplied by Santa Cruz Biotechnology, clone C4, sc-47778; anti-cyclin D3 (cyclin D3, 1:2,000) supplied by Cell Signaling Technology, clone DCS22, 2936S; rabbit anti-mouse IgG–HRP (1:20,000) supplied by Santa Cruz Biotechnology, sc2005; goat anti-rabbit IgG–HRP (1:20,000) supplied by Santa Cruz Biotechnology, sc2004; rabbit anti-goat IgG–HRP (1:20,000) supplied by Santa Cruz Biotechnology, sc-2768; anti-human SMAD3 (SMAD3, 10 μg in 100 μl for immunoprecipitation and 1:1,000 for western blot) supplied by Cell Signaling Technology, clone C67H9, 9523; anti-human GATA1 (GATA1, 10 μg in 100 μl) supplied by Abcam, ab11852; and normal rabbit IgG, (IgG, 1 μg in 100 μl) supplied by Cell Signaling Technology, 2729S. ..

Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria
Article Snippet: Recombinant TGFβ and TGFβ type I receptor inhibitor SB431542 were purchased from Sigma-Aldrich (T7039 and S4696). .. The following antibodies were used: PC7-conjugated anti-human CD235a (GPA–PC7, 1:50) supplied by Beckman Coulter, clone 11E4B-7-6, A71564; PE-conjugated anti-Band3 (Band 3–PE, 1:100) supplied by IBGRL, clone BRIC 6, 9439PE; APC-conjugated anti-CD49d (integrin α4–APC, 1:20) supplied by Beckman Coulter, clone HP2/1, B01682 ; anti-human GATA1 (GATA1, 1:10) supplied by Santa Cruz Biotechnology, clone N1, sc-266X; anti-influenza haemagglutinin epitope tag HA.11 (SMAD3–HA, 1:10) supplied by BioLegend, clone 16B12, MMS-101P; anti-human cyclin D3 (cyclin D3, 1:250) supplied by Santa Cruz Biotechnology, clone D-7, sc-6283; anti-human SMAD2 (SMAD2, 1:500) supplied by Santa Cruz Biotechnology, clone S-20, sc-6200; anti-β-actin (β-actin, 1:1,000) supplied by Santa Cruz Biotechnology, clone C4, sc-47778; anti-cyclin D3 (cyclin D3, 1:2,000) supplied by Cell Signaling Technology, clone DCS22, 2936S; rabbit anti-mouse IgG–HRP (1:20,000) supplied by Santa Cruz Biotechnology, sc2005; goat anti-rabbit IgG–HRP (1:20,000) supplied by Santa Cruz Biotechnology, sc2004; rabbit anti-goat IgG–HRP (1:20,000) supplied by Santa Cruz Biotechnology, sc-2768; anti-human SMAD3 (SMAD3, 10 μg in 100 μl for immunoprecipitation and 1:1,000 for western blot) supplied by Cell Signaling Technology, clone C67H9, 9523; anti-human GATA1 (GATA1, 10 μg in 100 μl) supplied by Abcam, ab11852; and normal rabbit IgG, (IgG, 1 μg in 100 μl) supplied by Cell Signaling Technology, 2729S. ..

Western Blot:

Article Title: McIdas localizes to centrioles and controls centriole numbers through PLK4-dependent phosphorylation
Article Snippet: The antibodies used for immunoprecipitation were: mouse anti-GFP (clones 7.1 & 13.1, Roche), mouse anti-HA (clone F-7, Santa Cruz Biotechnology), mouse anti-myc (clone 4A6, Merck) and rabbit anti-hMcIdas (Pefani et al, ). .. The primary and secondary antibodies used for western blot analysis were: mouse anti-GFP (1:1000, Roche), mouse anti-myc (1:1000, clone 4A6, Merck), mouse anti-PLK4 (1:500, Merck), rabbit anti-STIL (1:500, Abcam), mouse anti-HA (1:1000, Santa Cruz Biotechnology), mouse anti-Flag (1:1500, Sigma-Aldrich), rabbit anti-hMcIdas (Pefani et al, ), goat anti-Rabbit IgG-HRP (1:1000–1:3000, Merck Millipore) and goat anti-Mouse IgG-HRP (1:1000–1:3000, Merck Millipore). ..

Article Title: Reduced cyclin D3 expression in erythroid cells protects against malaria.
Article Snippet: .. 4 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used PC7-conjugated anti-human CD235a (GPA-PC7 1:50) supplied by Beckman Coulter, clone # 11E4B-7-6, catalog #A71564; PE-conjugated anti-Band3 (Band3-PE 1:100) supplied by IBGRL, clone # BRIC 6, catalog # 9439CSPE; APC-conjugated anti-CD49d (α-4 integrin-APC 1:20) supplied by Beckman Coulter, clone # HP2/1, catalog #B01682; Anti-human GATA1 (GATA1 1:10) supplied by Santa Cruz Biotechnology, clone #N1 catalog #sc-266X; Anti-influenza hemagglutinin epitope tag HA.11(SMAD3-HA 1:10), supplied by Biolegend, clone #16B12, catalog #MMS-101P; Anti-human CyclinD3 (CyclinD3 1:250) supplied by Santa Cruz Biotechnology, clone #D-7, catalog #sc-6283; Anti-human Smad2 (Smad2 1:500) supplied by Santa Cruz Biotechnology, clone #S-20, catalog #sc-6200; Anti-beta-actin (beta-actin 1:1000) supplied by Santa Cruz Biotechnology, clone #C4, catalog #sc-47778; Rabbit anti-mouse IgG-HRP (1:20000), supplied by Santa Cruz Biotechnology, catalog #sc2005; Goat anti-rabbit IgG-HRP(1:20000), supplied by Santa Cruz Biotechnology, catalog #sc2004; Rabbit anti-goat IgG-HRP (1:20000), supplied by Santa Cruz Biotechnology, catalog #sc-2768; Anti-human SMAD3 (SMAD3 10μg in 100 μL for IP and 1:1000 for WB) supplied by Cell Signaling Technology clone #C67H9, catalog #9523; Anti-human GATA1 (GATA1 10μg in 100 μL) supplied by Abcam catalog #ab11852; Normal Rabbit IgG, (IgG, 1μg in 100 μL), supplied by Cell Signaling Technology, catalog #2729S. ..

Chromatin Immunoprecipitation:

Flow Cytometry:

Magnetic Resonance Imaging:

Incubation:

Article Title: MicroRNA-601 Enhances Osimertinib Sensitivity by Targeting Transient Receptor Potential Mucolipin 3 in Non-small Cell Lung Cancer Cells
Article Snippet: Subsequently, the blocked membranes were probed overnight at 4°C with primary antibodies specific for TRPML3 (1:1,000, ProteinTech), poly(ADP-ribose) polymerase (PARP; 1:1,000, Cell Signaling Technology), and GAPDH (1:1,000, BioSS). .. For secondary antibody detection, the membranes were incubated for 1 hour at 26°C in a 1:2,500 dilution of HRP-conjugated anti-rabbit or anti-mouse IgG (Santa Cruz Biotechnology). .. Immunoreactive bands were visualized via enhanced chemiluminescence on an Azure Biosystems C300 imager and quantified with AzureSpot v2.0 software (Azure Biosystems).

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