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maxwell rsc simply rna cells kit  (Promega)

 
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    Promega maxwell rsc simply rna cells kit
    Maxwell Rsc Simply Rna Cells Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simplis/maxwell+16+blood+dna+purification+kit/pmc12220080-70-9-16
    Average 90 stars, based on 1 article reviews
    maxwell rsc simply rna cells kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Nucleic Acid Purification:

    Article Title: Early detection of SARS-CoV-2 variants using genomic surveillance: insights from aircraft wastewater and nasal swabs at Kigali International Airport, Rwanda
    Article Snippet: Particles (Ceres Nanosciences, Inc.) were taken from 1.2 ml of the sample and extracted using the Magmax Microbiome Ultra Nucleic Acid Kit (Thermo Fisher Scientific) on the semi-automated Kingfisher Flex Purification System with a 96 Head version 1.01.0 (Thermo Fisher Scientific, USA). .. Pooled nasal swab samples (n = 5-10 per pool) were extracted using Maxwell RSC Viral Total Nucleic Acid Purification Kits (Promega) on the benchtop automated Maxwell RSC 48 DNA/RNA extraction platform (Promega, USA). ..

    Extraction:

    Article Title: Early detection of SARS-CoV-2 variants using genomic surveillance: insights from aircraft wastewater and nasal swabs at Kigali International Airport, Rwanda
    Article Snippet: Particles (Ceres Nanosciences, Inc.) were taken from 1.2 ml of the sample and extracted using the Magmax Microbiome Ultra Nucleic Acid Kit (Thermo Fisher Scientific) on the semi-automated Kingfisher Flex Purification System with a 96 Head version 1.01.0 (Thermo Fisher Scientific, USA). .. Pooled nasal swab samples (n = 5-10 per pool) were extracted using Maxwell RSC Viral Total Nucleic Acid Purification Kits (Promega) on the benchtop automated Maxwell RSC 48 DNA/RNA extraction platform (Promega, USA). ..

    RNA Extraction:

    Article Title: PNAS Template for Supporting Information Supporting Information for Ago2 and a miRNA reduce Topoisomerase 1 for enhancing DNA cleavage in antibody diversification by activation-induced cytidine deaminase
    Article Snippet: Optical density was measured at a wavelength of 254 nm using a SpectraMax (Molecular Device). .. Eight serial fractions obtained by the fractionator were pooled for RNA extraction using the kit of Maxwell RSC miRNA from plasma and serum (Promega). cDNA was synthesized from these RNA samples by SuperScript IV (LifeTechnologies) and oligo dT20 (LifeTechnologies). ..

    Clinical Proteomics:

    Article Title: PNAS Template for Supporting Information Supporting Information for Ago2 and a miRNA reduce Topoisomerase 1 for enhancing DNA cleavage in antibody diversification by activation-induced cytidine deaminase
    Article Snippet: Optical density was measured at a wavelength of 254 nm using a SpectraMax (Molecular Device). .. Eight serial fractions obtained by the fractionator were pooled for RNA extraction using the kit of Maxwell RSC miRNA from plasma and serum (Promega). cDNA was synthesized from these RNA samples by SuperScript IV (LifeTechnologies) and oligo dT20 (LifeTechnologies). ..

    Synthesized:

    Article Title: PNAS Template for Supporting Information Supporting Information for Ago2 and a miRNA reduce Topoisomerase 1 for enhancing DNA cleavage in antibody diversification by activation-induced cytidine deaminase
    Article Snippet: Optical density was measured at a wavelength of 254 nm using a SpectraMax (Molecular Device). .. Eight serial fractions obtained by the fractionator were pooled for RNA extraction using the kit of Maxwell RSC miRNA from plasma and serum (Promega). cDNA was synthesized from these RNA samples by SuperScript IV (LifeTechnologies) and oligo dT20 (LifeTechnologies). ..

    other:

    Article Title:
    Article Snippet: 1 Supplementary File 1: The Promega Maxwell® RSC 48 Instrument and RSC Blood DNA Kit Protocol 1.

    Article Title: PNAS Template for Supporting Information Supporting Information for Ago2 and a miRNA reduce Topoisomerase 1 for enhancing DNA cleavage in antibody diversification by activation-induced cytidine deaminase
    Article Snippet: RNA was purified by Sepazol RNA Super II (Nacalaitesque) or Maxwell® RSC simplyRNA Cells Kit (Promega). cDNA was synthesized using SuperScript® IV Reverse Transcriptase (ThermoFisher Scientific).

    Article Title:
    Article Snippet: RNA extraction from cells was performed using theMaxwell RSC simplyRNATissue Kit (Promega, Madison,WI) according to themanufacturer’s instructions.

    Gene Expression:

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.

    Isolation:

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.

    Virus:

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.

    Reverse Transcription:

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.

    Polymerase Chain Reaction:

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.

    Article Title: FOXP3 expression diversifies the metabolic capacity and enhances the efficacy of CD8 T cells in adoptive immunotherapy of melanoma
    Article Snippet: .. Ova and Thy1.1 gene expression analysis RNA isolated from tumors (Maxwell 16 LEV simplyRNA tissue kit, Promega) (1 μg/sample) was treated with DNase I (Invitrogen) prior to RT with Moloney murine leukemia virus reverse transcriptase (Invitrogen) in the presence of RNaseOUT (Invitrogen) and 1/4 of cDNA preparation was used for each PCR. .. QPCR was performed with 200 nM of validated primers in 25 μl reactions using the Platinum SYBR Green One-Step qPCR kit (Invitrogen) and the following program: 50°C for 2 min, 95°C for 10 min, and 40 cycles of amplification at 95°C for 15 s and 62°C for 60 s. Relative levels of target mRNA were compared with Gapdh using the 2^ΔΔCt method, where the control cell type or treatment group was normalized to 1.



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