sigmaplot 8.0 (SPSS Inc)
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SPSS Inc
sigmaplot 8.0
Sigmaplot 8.0, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigmaplot/sigmaplot+8+0/pmc02615518-122-17-19
Average 90 stars, based on 1 article reviews
Sigmaplot 8.0, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sigmaplot/sigmaplot+8+0/pmc02615518-122-17-19
Average 90 stars, based on 1 article reviews
sigmaplot 8.0 - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "RecR-mediated Modulation of RecF Dimer Specificity for Single- and
Double-stranded
DNA * S⃞ "
Article Title: RecR-mediated Modulation of RecF Dimer Specificity for Single- and
Double-stranded
DNA
Journal:
doi: 10.1074/jbc.M806378200
Figure Legend Snippet: FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Techniques Used: Titration, Labeling, Software, Derivative Assay
Figure Legend Snippet: RecF-DNA interaction measured by the fluorescence polarization assay. The labeled DNA substrates (20 nm) were titrated with RecF in the presence of 2 mm nucleotide. RecR was included where indicated at final concentration of 30 μm. Normalized fluorescence polarization signal in arbitrary units was plotted against protein concentrations. The graphs were created with SigmaPlot 8.0 (SPSS Inc.). RecF interactions with ssDNA (S1) are shown in panel A, with dsDNA (D1) in panel B, and with ds/ssDNA (J1) junction in panel C. Dotted lines represent the interaction of the RecF(S279R) mutant with DNA in the presence of ATP; short-dashed lines, a wild-type RecF in the presence of ADP; solid lines, a wild-type RecF in the presence of ATP; long-dashed lines, a wild-type RecF in the presence of ATP and RecR.
Techniques Used: Fluorescence, Labeling, Concentration Assay, Mutagenesis
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