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digitonin thermo fischer scientific  (Thermo Fisher)


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    Structured Review

    Thermo Fisher digitonin thermo fischer scientific
    Digitonin Thermo Fischer Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigma+plot+version+13+0+software/Digitonin/pm41528844-784-86-87
    Average 97 stars, based on 1 article reviews
    digitonin thermo fischer scientific - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Lysis:

    Article Title: FLNB and TTC26 regulate ciliary Hedgehog signaling to maintain intervertebral disc matrix homeostasis in adolescent idiopathic scoliosis.
    Article Snippet: .. Briefly, cells were lysed in lysis buffer (25 mM Tris-HCl, pH7.4, 150 mM NaCl, 0.4% digitonin, 1 mM EGTA) or RIPA (Thermo Fisher Scientific) buffer with complete protease inhibitor cocktail (PI, Roche). ..

    Protease Inhibitor:

    Article Title: FLNB and TTC26 regulate ciliary Hedgehog signaling to maintain intervertebral disc matrix homeostasis in adolescent idiopathic scoliosis.
    Article Snippet: .. Briefly, cells were lysed in lysis buffer (25 mM Tris-HCl, pH7.4, 150 mM NaCl, 0.4% digitonin, 1 mM EGTA) or RIPA (Thermo Fisher Scientific) buffer with complete protease inhibitor cocktail (PI, Roche). ..

    Incubation:

    Article Title: Sex-Dimorphic Neural Memory Shapes Pancreatic Tissue Resilience
    Article Snippet: Oligos (5’-3’ – IDT 25nm, standard desalting): Tn5_1 – TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG Tn5_2_ME_comp – GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG TN5MEREV - /5Phos/CTGTCTCTTATACACATCT .. 50×10 single live acinar cells (previously optimized cell number) were centrifuged at 500g for 4 minutes at 4°C and resuspended in 50 μl of ATAC resuspension buffer (RSB) (10mM Tris-HCL (Sigma) pH7.4, 10mM NaCl (Sigma), 3mM MgCl 2 (Sigma)) with 0.1% NP40 (Thermo Fisher), 0.1% Tween-20 (Sigma) and 0.01% digitonin (Thermo Fisher) for a 4 minute incubation on ice (optimize for sorted acinar cells). ..

    Purification:

    Article Title: Structure of giant kelp Photosystem I-FCP uncovers drivers of antenna evolution across the red lineage
    Article Snippet: .. Using digitonin and sucrose gradients, we purified PSI-FCP for structural determination with cryogenic electron microscopy (cryoEM) (Supplementary Figs. – , Supplementary Tables – ). ..

    Membrane:

    Article Title: Mitochondrial integrated stress response activation creates a therapeutic vulnerability to MCL-1 inhibition in acute myeloid leukemia.
    Article Snippet: Briefly, 500,000 cells were seeded and treated with either DMSO or 50 nM IACS for 24 h. Cells were collected, washed with PBS and stained with AquaZombie viability dye (Biolegend). .. Cells were exposed to a 0.0001% digitonin (Thermo Fisher Scientific) for membrane permeabilization and then exposed to concentrations increasing up to 5 M of human BIM peptide (sequence: MRPEIWIAQELRRIGDEFNA) for 1 h at 23 C. After BIM peptide exposure, 4% formaldehyde in PBS (Thermo Fisher Scientific) was added to terminate the peptide exposure, followed by neutralization with N2 buffer (1.7M Tris base, 1.25M glycine, pH 9.1). .. The cells were stained with 1:40 with anti-cytochrome c antibody (clone 6H2.B4, Biolegend) overnight.

    Sequencing:

    Article Title: Mitochondrial integrated stress response activation creates a therapeutic vulnerability to MCL-1 inhibition in acute myeloid leukemia.
    Article Snippet: Briefly, 500,000 cells were seeded and treated with either DMSO or 50 nM IACS for 24 h. Cells were collected, washed with PBS and stained with AquaZombie viability dye (Biolegend). .. Cells were exposed to a 0.0001% digitonin (Thermo Fisher Scientific) for membrane permeabilization and then exposed to concentrations increasing up to 5 M of human BIM peptide (sequence: MRPEIWIAQELRRIGDEFNA) for 1 h at 23 C. After BIM peptide exposure, 4% formaldehyde in PBS (Thermo Fisher Scientific) was added to terminate the peptide exposure, followed by neutralization with N2 buffer (1.7M Tris base, 1.25M glycine, pH 9.1). .. The cells were stained with 1:40 with anti-cytochrome c antibody (clone 6H2.B4, Biolegend) overnight.

    Neutralization:

    Article Title: Mitochondrial integrated stress response activation creates a therapeutic vulnerability to MCL-1 inhibition in acute myeloid leukemia.
    Article Snippet: Briefly, 500,000 cells were seeded and treated with either DMSO or 50 nM IACS for 24 h. Cells were collected, washed with PBS and stained with AquaZombie viability dye (Biolegend). .. Cells were exposed to a 0.0001% digitonin (Thermo Fisher Scientific) for membrane permeabilization and then exposed to concentrations increasing up to 5 M of human BIM peptide (sequence: MRPEIWIAQELRRIGDEFNA) for 1 h at 23 C. After BIM peptide exposure, 4% formaldehyde in PBS (Thermo Fisher Scientific) was added to terminate the peptide exposure, followed by neutralization with N2 buffer (1.7M Tris base, 1.25M glycine, pH 9.1). .. The cells were stained with 1:40 with anti-cytochrome c antibody (clone 6H2.B4, Biolegend) overnight.

    Clear Native PAGE:

    Article Title: Activation of AMPK as a therapeutic strategy for FBXL4-related mitochondrial DNA depletion syndrome.
    Article Snippet: .. For BN-PAGE analysis, the mitochondria-enriched pellet was processed in 1× Native PAGE buffer (Thermo Fisher #BN2003) supplemented with 1% digitonin (Thermo Fisher #BN2006), 1 U/μl Benzonase (Merck #E1014) and 2 mM MgCl2. ..



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