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shctrl shrna construct (shc002)  (Millipore)


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    Structured Review

    Millipore shctrl shrna construct (shc002)
    Shctrl Shrna Construct (Shc002), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+construct+shctrl/non+targeting+shrna/us10308698-333-1-9
    Average 90 stars, based on 1 article reviews
    shctrl shrna construct (shc002) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (sh NONT ) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (shNONT) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes
    Article Snippet: Cells were incubated in a humidity-controlled environment at 37°C with 5% CO 2 (Thermo Fisher Scientific Forma 371) and were used up to 10 passages. pGenLenti-empty, pGenLenti-CDKN1A, pGenLenti-CDKN2A, and pGenLenti-NFE2L2 were obtained from Genscript. pMXs-FLAG-SLC25A51, pMXs-FLAG-SLC25A51-K91A mutant, and pMXs-HA-GFP vectors are from a previous study . pMXs-FLAG-SLC25A38 and pMXs-SOD2-CAT-GFP vectors were generated in this study. pLKO.1-tGFP-shNTC (non-targeting control, SHC016) and pLKO.1-tGFP-shSLC25A51 were purchased from Mission shRNA Custom DNA (Millipore Sigma).

    Article Title: Glutamine metabolism is essential for coronavirus replication in host cells and in mice
    Article Snippet: The following shRNA and siRNA constructs pLKO.1-puro shRNA control plasmid (Cat. SHC002), pLKO.1-TRCN0000051135 targeting GLS (Clone ID: NM_014905.2–1441s1c1), and pLKO.1-TRCN0000298987 targeting GLS (Clone ID: 014,905.3–1475s21c1), were from Millipore Sigma.

    Article Title: Inhibition of CD38 by 78c Enhanced NAD + , Alleviated Inflammation, and Decreased Oxidative Stress in Old Murine Macrophages Induced by Oral Pathogens.
    Article Snippet: Lentiviral vectors were obtained by ultracentrifation at 25,000 rpm for 1.5 h at 4 ◦C using a Beckman ultracentrifue (Beckman Coulter, Indianapolis, IN, USA) and followed by resuspension of viral pellets in serum-free DMEM media.

    Article Title: Activation of the endoplasmic reticulum stress regulator IRE1α compromises pulmonary host defenses.
    Article Snippet: The mouse Xbp1 specific shRNA plasmid and the non-target control shRNA plasmid (Cat# SHC016) were purchased from Millipore Sigma (Table S2).


    Article Title: A CHD8-TRRAP axis facilitates MYC and E2F target gene regulation in human neural stem cells.
    Article Snippet: A PLKO.1-puroControl-shRNA (MISSION pLKO.1-puro Non-Mammalian shRNA, SHC002, 5’- CAACAAGATGAAGAGCACCAA-3’, SigmaAldrich) was used to perform the control experiments in the same manner.

    shRNA:

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (sh NONT ) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (shNONT) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes
    Article Snippet: Cells were incubated in a humidity-controlled environment at 37°C with 5% CO 2 (Thermo Fisher Scientific Forma 371) and were used up to 10 passages. pGenLenti-empty, pGenLenti-CDKN1A, pGenLenti-CDKN2A, and pGenLenti-NFE2L2 were obtained from Genscript. pMXs-FLAG-SLC25A51, pMXs-FLAG-SLC25A51-K91A mutant, and pMXs-HA-GFP vectors are from a previous study . pMXs-FLAG-SLC25A38 and pMXs-SOD2-CAT-GFP vectors were generated in this study. pLKO.1-tGFP-shNTC (non-targeting control, SHC016) and pLKO.1-tGFP-shSLC25A51 were purchased from Mission shRNA Custom DNA (Millipore Sigma).

    Article Title: Glutamine metabolism is essential for coronavirus replication in host cells and in mice
    Article Snippet: The following shRNA and siRNA constructs pLKO.1-puro shRNA control plasmid (Cat. SHC002), pLKO.1-TRCN0000051135 targeting GLS (Clone ID: NM_014905.2–1441s1c1), and pLKO.1-TRCN0000298987 targeting GLS (Clone ID: 014,905.3–1475s21c1), were from Millipore Sigma.

    Article Title: Inhibition of CD38 by 78c Enhanced NAD + , Alleviated Inflammation, and Decreased Oxidative Stress in Old Murine Macrophages Induced by Oral Pathogens.
    Article Snippet: Lentiviral vectors were obtained by ultracentrifation at 25,000 rpm for 1.5 h at 4 ◦C using a Beckman ultracentrifue (Beckman Coulter, Indianapolis, IN, USA) and followed by resuspension of viral pellets in serum-free DMEM media.

    Article Title: Activation of the endoplasmic reticulum stress regulator IRE1α compromises pulmonary host defenses.
    Article Snippet: The mouse Xbp1 specific shRNA plasmid and the non-target control shRNA plasmid (Cat# SHC016) were purchased from Millipore Sigma (Table S2).


    Article Title: A CHD8-TRRAP axis facilitates MYC and E2F target gene regulation in human neural stem cells.
    Article Snippet: A PLKO.1-puroControl-shRNA (MISSION pLKO.1-puro Non-Mammalian shRNA, SHC002, 5’- CAACAAGATGAAGAGCACCAA-3’, SigmaAldrich) was used to perform the control experiments in the same manner.

    Plasmid Preparation:

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (sh NONT ) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (shNONT) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes
    Article Snippet: Cells were incubated in a humidity-controlled environment at 37°C with 5% CO 2 (Thermo Fisher Scientific Forma 371) and were used up to 10 passages. pGenLenti-empty, pGenLenti-CDKN1A, pGenLenti-CDKN2A, and pGenLenti-NFE2L2 were obtained from Genscript. pMXs-FLAG-SLC25A51, pMXs-FLAG-SLC25A51-K91A mutant, and pMXs-HA-GFP vectors are from a previous study . pMXs-FLAG-SLC25A38 and pMXs-SOD2-CAT-GFP vectors were generated in this study. pLKO.1-tGFP-shNTC (non-targeting control, SHC016) and pLKO.1-tGFP-shSLC25A51 were purchased from Mission shRNA Custom DNA (Millipore Sigma).

    Article Title: Glutamine metabolism is essential for coronavirus replication in host cells and in mice
    Article Snippet: The following shRNA and siRNA constructs pLKO.1-puro shRNA control plasmid (Cat. SHC002), pLKO.1-TRCN0000051135 targeting GLS (Clone ID: NM_014905.2–1441s1c1), and pLKO.1-TRCN0000298987 targeting GLS (Clone ID: 014,905.3–1475s21c1), were from Millipore Sigma.

    Article Title: Inhibition of CD38 by 78c Enhanced NAD + , Alleviated Inflammation, and Decreased Oxidative Stress in Old Murine Macrophages Induced by Oral Pathogens.
    Article Snippet: Lentiviral vectors were obtained by ultracentrifation at 25,000 rpm for 1.5 h at 4 ◦C using a Beckman ultracentrifue (Beckman Coulter, Indianapolis, IN, USA) and followed by resuspension of viral pellets in serum-free DMEM media.

    Article Title: Activation of the endoplasmic reticulum stress regulator IRE1α compromises pulmonary host defenses.
    Article Snippet: The mouse Xbp1 specific shRNA plasmid and the non-target control shRNA plasmid (Cat# SHC016) were purchased from Millipore Sigma (Table S2).


    Article Title: A CHD8-TRRAP axis facilitates MYC and E2F target gene regulation in human neural stem cells.
    Article Snippet: A PLKO.1-puroControl-shRNA (MISSION pLKO.1-puro Non-Mammalian shRNA, SHC002, 5’- CAACAAGATGAAGAGCACCAA-3’, SigmaAldrich) was used to perform the control experiments in the same manner.

    Control:

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (sh NONT ) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (shNONT) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes
    Article Snippet: Cells were incubated in a humidity-controlled environment at 37°C with 5% CO 2 (Thermo Fisher Scientific Forma 371) and were used up to 10 passages. pGenLenti-empty, pGenLenti-CDKN1A, pGenLenti-CDKN2A, and pGenLenti-NFE2L2 were obtained from Genscript. pMXs-FLAG-SLC25A51, pMXs-FLAG-SLC25A51-K91A mutant, and pMXs-HA-GFP vectors are from a previous study . pMXs-FLAG-SLC25A38 and pMXs-SOD2-CAT-GFP vectors were generated in this study. pLKO.1-tGFP-shNTC (non-targeting control, SHC016) and pLKO.1-tGFP-shSLC25A51 were purchased from Mission shRNA Custom DNA (Millipore Sigma).

    Article Title: Glutamine metabolism is essential for coronavirus replication in host cells and in mice
    Article Snippet: The following shRNA and siRNA constructs pLKO.1-puro shRNA control plasmid (Cat. SHC002), pLKO.1-TRCN0000051135 targeting GLS (Clone ID: NM_014905.2–1441s1c1), and pLKO.1-TRCN0000298987 targeting GLS (Clone ID: 014,905.3–1475s21c1), were from Millipore Sigma.

    Article Title: Inhibition of CD38 by 78c Enhanced NAD + , Alleviated Inflammation, and Decreased Oxidative Stress in Old Murine Macrophages Induced by Oral Pathogens.
    Article Snippet: Lentiviral vectors were obtained by ultracentrifation at 25,000 rpm for 1.5 h at 4 ◦C using a Beckman ultracentrifue (Beckman Coulter, Indianapolis, IN, USA) and followed by resuspension of viral pellets in serum-free DMEM media.

    Article Title: Activation of the endoplasmic reticulum stress regulator IRE1α compromises pulmonary host defenses.
    Article Snippet: The mouse Xbp1 specific shRNA plasmid and the non-target control shRNA plasmid (Cat# SHC016) were purchased from Millipore Sigma (Table S2).


    Article Title: A CHD8-TRRAP axis facilitates MYC and E2F target gene regulation in human neural stem cells.
    Article Snippet: A PLKO.1-puroControl-shRNA (MISSION pLKO.1-puro Non-Mammalian shRNA, SHC002, 5’- CAACAAGATGAAGAGCACCAA-3’, SigmaAldrich) was used to perform the control experiments in the same manner.

    Incubation:

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (sh NONT ) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (shNONT) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes
    Article Snippet: Cells were incubated in a humidity-controlled environment at 37°C with 5% CO 2 (Thermo Fisher Scientific Forma 371) and were used up to 10 passages. pGenLenti-empty, pGenLenti-CDKN1A, pGenLenti-CDKN2A, and pGenLenti-NFE2L2 were obtained from Genscript. pMXs-FLAG-SLC25A51, pMXs-FLAG-SLC25A51-K91A mutant, and pMXs-HA-GFP vectors are from a previous study . pMXs-FLAG-SLC25A38 and pMXs-SOD2-CAT-GFP vectors were generated in this study. pLKO.1-tGFP-shNTC (non-targeting control, SHC016) and pLKO.1-tGFP-shSLC25A51 were purchased from Mission shRNA Custom DNA (Millipore Sigma).

    Article Title: Glutamine metabolism is essential for coronavirus replication in host cells and in mice
    Article Snippet: The following shRNA and siRNA constructs pLKO.1-puro shRNA control plasmid (Cat. SHC002), pLKO.1-TRCN0000051135 targeting GLS (Clone ID: NM_014905.2–1441s1c1), and pLKO.1-TRCN0000298987 targeting GLS (Clone ID: 014,905.3–1475s21c1), were from Millipore Sigma.

    Article Title: Inhibition of CD38 by 78c Enhanced NAD + , Alleviated Inflammation, and Decreased Oxidative Stress in Old Murine Macrophages Induced by Oral Pathogens.
    Article Snippet: Lentiviral vectors were obtained by ultracentrifation at 25,000 rpm for 1.5 h at 4 ◦C using a Beckman ultracentrifue (Beckman Coulter, Indianapolis, IN, USA) and followed by resuspension of viral pellets in serum-free DMEM media.

    Article Title: Activation of the endoplasmic reticulum stress regulator IRE1α compromises pulmonary host defenses.
    Article Snippet: The mouse Xbp1 specific shRNA plasmid and the non-target control shRNA plasmid (Cat# SHC016) were purchased from Millipore Sigma (Table S2).


    Article Title: A CHD8-TRRAP axis facilitates MYC and E2F target gene regulation in human neural stem cells.
    Article Snippet: A PLKO.1-puroControl-shRNA (MISSION pLKO.1-puro Non-Mammalian shRNA, SHC002, 5’- CAACAAGATGAAGAGCACCAA-3’, SigmaAldrich) was used to perform the control experiments in the same manner.

    Mutagenesis:

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (sh NONT ) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (shNONT) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes
    Article Snippet: Cells were incubated in a humidity-controlled environment at 37°C with 5% CO 2 (Thermo Fisher Scientific Forma 371) and were used up to 10 passages. pGenLenti-empty, pGenLenti-CDKN1A, pGenLenti-CDKN2A, and pGenLenti-NFE2L2 were obtained from Genscript. pMXs-FLAG-SLC25A51, pMXs-FLAG-SLC25A51-K91A mutant, and pMXs-HA-GFP vectors are from a previous study . pMXs-FLAG-SLC25A38 and pMXs-SOD2-CAT-GFP vectors were generated in this study. pLKO.1-tGFP-shNTC (non-targeting control, SHC016) and pLKO.1-tGFP-shSLC25A51 were purchased from Mission shRNA Custom DNA (Millipore Sigma).

    Article Title: Glutamine metabolism is essential for coronavirus replication in host cells and in mice
    Article Snippet: The following shRNA and siRNA constructs pLKO.1-puro shRNA control plasmid (Cat. SHC002), pLKO.1-TRCN0000051135 targeting GLS (Clone ID: NM_014905.2–1441s1c1), and pLKO.1-TRCN0000298987 targeting GLS (Clone ID: 014,905.3–1475s21c1), were from Millipore Sigma.

    Article Title: Inhibition of CD38 by 78c Enhanced NAD + , Alleviated Inflammation, and Decreased Oxidative Stress in Old Murine Macrophages Induced by Oral Pathogens.
    Article Snippet: Lentiviral vectors were obtained by ultracentrifation at 25,000 rpm for 1.5 h at 4 ◦C using a Beckman ultracentrifue (Beckman Coulter, Indianapolis, IN, USA) and followed by resuspension of viral pellets in serum-free DMEM media.

    Article Title: Activation of the endoplasmic reticulum stress regulator IRE1α compromises pulmonary host defenses.
    Article Snippet: The mouse Xbp1 specific shRNA plasmid and the non-target control shRNA plasmid (Cat# SHC016) were purchased from Millipore Sigma (Table S2).


    Article Title: A CHD8-TRRAP axis facilitates MYC and E2F target gene regulation in human neural stem cells.
    Article Snippet: A PLKO.1-puroControl-shRNA (MISSION pLKO.1-puro Non-Mammalian shRNA, SHC002, 5’- CAACAAGATGAAGAGCACCAA-3’, SigmaAldrich) was used to perform the control experiments in the same manner.

    Generated:

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (sh NONT ) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (shNONT) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes
    Article Snippet: Cells were incubated in a humidity-controlled environment at 37°C with 5% CO 2 (Thermo Fisher Scientific Forma 371) and were used up to 10 passages. pGenLenti-empty, pGenLenti-CDKN1A, pGenLenti-CDKN2A, and pGenLenti-NFE2L2 were obtained from Genscript. pMXs-FLAG-SLC25A51, pMXs-FLAG-SLC25A51-K91A mutant, and pMXs-HA-GFP vectors are from a previous study . pMXs-FLAG-SLC25A38 and pMXs-SOD2-CAT-GFP vectors were generated in this study. pLKO.1-tGFP-shNTC (non-targeting control, SHC016) and pLKO.1-tGFP-shSLC25A51 were purchased from Mission shRNA Custom DNA (Millipore Sigma).

    Article Title: Glutamine metabolism is essential for coronavirus replication in host cells and in mice
    Article Snippet: The following shRNA and siRNA constructs pLKO.1-puro shRNA control plasmid (Cat. SHC002), pLKO.1-TRCN0000051135 targeting GLS (Clone ID: NM_014905.2–1441s1c1), and pLKO.1-TRCN0000298987 targeting GLS (Clone ID: 014,905.3–1475s21c1), were from Millipore Sigma.

    Article Title: Inhibition of CD38 by 78c Enhanced NAD + , Alleviated Inflammation, and Decreased Oxidative Stress in Old Murine Macrophages Induced by Oral Pathogens.
    Article Snippet: Lentiviral vectors were obtained by ultracentrifation at 25,000 rpm for 1.5 h at 4 ◦C using a Beckman ultracentrifue (Beckman Coulter, Indianapolis, IN, USA) and followed by resuspension of viral pellets in serum-free DMEM media.

    Article Title: Activation of the endoplasmic reticulum stress regulator IRE1α compromises pulmonary host defenses.
    Article Snippet: The mouse Xbp1 specific shRNA plasmid and the non-target control shRNA plasmid (Cat# SHC016) were purchased from Millipore Sigma (Table S2).


    Article Title: A CHD8-TRRAP axis facilitates MYC and E2F target gene regulation in human neural stem cells.
    Article Snippet: A PLKO.1-puroControl-shRNA (MISSION pLKO.1-puro Non-Mammalian shRNA, SHC002, 5’- CAACAAGATGAAGAGCACCAA-3’, SigmaAldrich) was used to perform the control experiments in the same manner.

    Construct:

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (sh NONT ) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: An epigenetic pathway regulates MHC-II expression and function in B cell lymphoma models
    Article Snippet: The clone ID for mouse Ciita was TRCN0000086452, and the nontargeting (shNONT) shRNA construct (SHC002) was purchased from MilliporeSigma.

    Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes
    Article Snippet: Cells were incubated in a humidity-controlled environment at 37°C with 5% CO 2 (Thermo Fisher Scientific Forma 371) and were used up to 10 passages. pGenLenti-empty, pGenLenti-CDKN1A, pGenLenti-CDKN2A, and pGenLenti-NFE2L2 were obtained from Genscript. pMXs-FLAG-SLC25A51, pMXs-FLAG-SLC25A51-K91A mutant, and pMXs-HA-GFP vectors are from a previous study . pMXs-FLAG-SLC25A38 and pMXs-SOD2-CAT-GFP vectors were generated in this study. pLKO.1-tGFP-shNTC (non-targeting control, SHC016) and pLKO.1-tGFP-shSLC25A51 were purchased from Mission shRNA Custom DNA (Millipore Sigma).

    Article Title: Glutamine metabolism is essential for coronavirus replication in host cells and in mice
    Article Snippet: The following shRNA and siRNA constructs pLKO.1-puro shRNA control plasmid (Cat. SHC002), pLKO.1-TRCN0000051135 targeting GLS (Clone ID: NM_014905.2–1441s1c1), and pLKO.1-TRCN0000298987 targeting GLS (Clone ID: 014,905.3–1475s21c1), were from Millipore Sigma.

    Article Title: Inhibition of CD38 by 78c Enhanced NAD + , Alleviated Inflammation, and Decreased Oxidative Stress in Old Murine Macrophages Induced by Oral Pathogens.
    Article Snippet: Lentiviral vectors were obtained by ultracentrifation at 25,000 rpm for 1.5 h at 4 ◦C using a Beckman ultracentrifue (Beckman Coulter, Indianapolis, IN, USA) and followed by resuspension of viral pellets in serum-free DMEM media.

    Article Title: Activation of the endoplasmic reticulum stress regulator IRE1α compromises pulmonary host defenses.
    Article Snippet: The mouse Xbp1 specific shRNA plasmid and the non-target control shRNA plasmid (Cat# SHC016) were purchased from Millipore Sigma (Table S2).


    Article Title: A CHD8-TRRAP axis facilitates MYC and E2F target gene regulation in human neural stem cells.
    Article Snippet: A PLKO.1-puroControl-shRNA (MISSION pLKO.1-puro Non-Mammalian shRNA, SHC002, 5’- CAACAAGATGAAGAGCACCAA-3’, SigmaAldrich) was used to perform the control experiments in the same manner.



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    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based <t>shRNA</t> silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of <t>normalized</t> <t>shCTRL</t> samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.
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    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based <t>shRNA</t> silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of <t>normalized</t> <t>shCTRL</t> samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.
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    Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based shRNA silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of normalized shCTRL samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.

    Journal: Cancers

    Article Title: Dual Role of Integrin Alpha-6 in Glioblastoma: Supporting Stemness in Proneural Stem-Like Cells While Inducing Radioresistance in Mesenchymal Stem-Like Cells

    doi: 10.3390/cancers13123055

    Figure Lengend Snippet: Silencing of integrin a6 in MES-GSCs does not have an impact on stemness and self-renewal. ( A , C ) Representative Western blot of the lentiviral-based shRNA silencing of ITGA6 in PN-GIC2 ( A ) and MES-GSCs ( C ). The values indicated within blots are relative to the densitometric analysis. Numbers indicate the normalized integrin a6 intensity ratio relative to the mean of normalized shCTRL samples. ( B ) Assessment of self-renewal capacity (mean ± SEM; n = 3; unpaired t -test) and sphere size (mean ± SEM; n = 3; Mann–Whitney test) in PN-GSCs silenced for ITGA6 . Micrographs from representative fields of gliomaspheres (scale bar = 100 µm). ( D ) Self-renewal capacity (mean ± SEM; n = 4; unpaired t -test) and sphere size (mean ± SEM; n = 4; Mann–Whitney test) in MES-GSCs silenced for ITGA6 . The number of independent experiments— n , the number of gliomaspheres scored for each condition—N. The values specified within the bar plot indicate the mean relative self-renewal capacity. ( E ) Transcript levels of ITGA6 and NES detected by means of qPCR in shITGA6 PN-GSCs and MES-GSCs (mean ± SEM; n = 3; unpaired t -test). ( F ) Relative transcript amount of ALDH1A3 , a specific MES stem cell marker, in shITGA6 MES-GSCs (mean ± SEM; n = 3; unpaired t -test). *** p < 0.001; **** p < 0.0001.

    Article Snippet: Non-targeting shRNA construct (shCTRL; source clone ID: RHS4348) and shITGA6 plasmid mapping against ITGA6 exon 15 (target sequence: AGGATATTGCTTTAGAAAT; source clone ID: V3LHS_326014) were purchased from Thermo-Scientific.

    Techniques: Western Blot, shRNA, MANN-WHITNEY, Marker