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sharp borosilicate electrodes  (Sutter Instrument Company)


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    Sutter Instrument Company sharp borosilicate electrodes
    Sharp Borosilicate Electrodes, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sharp+borosilicate+electrodes/micropipette+puller+p+97/pm39721493-81-22-38
    Average 90 stars, based on 1 article reviews
    sharp borosilicate electrodes - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Connectivity of a Conspicuous Layered Structure in the Dorsal Telencephalon of the Peacock Gudgeon, Tateurndina ocellicauda
    Article Snippet: The tissue was then dipped into ice‐cold Ringer's solution and washed in Ringer's solution twice for 10 min. A fluorescent stereomicroscope (Nikon SMZ 25, Minato City, Tokyo, Japan) was used to visualize the respective tracer application sites. .. Injections were performed by pulling glass microelectrodes with a micropipette puller (P‐87, Sutter Instruments, California, USA) using borosilicate glass with filament (GB150F‐8P, Science Products, Hofheim, Germany) and with their tips broken back under a microscope. .. These were filled with 10% neurobiotin (Vector Laboratories Inc., Burlingame, CA, USA) in 0.5 M potassium acetate containing 0.05% pyranine (Thermo Fisher, Kandel, Germany) to visualize the electrode tip (10 MΩ resistance).

    Article Title: Connectivity of a Conspicuous Layered Structure in the Dorsal Telencephalon of the Peacock Gudgeon, Tateurndina ocellicauda.
    Article Snippet: The tissue was then dipped into ice-cold Ringer’s solution and washed in Ringer’s solution twice for 10 min. A fluorescent stereomicroscope (Nikon SMZ 25, Minato City, Tokyo, Japan) was used to visualize the respective tracer application sites. .. Injections were performed by pulling glass microelectrodes with a micropipette puller (P-87, Sutter Instruments, California, USA) using borosilicate glass with filament (GB150F-8P, Science Products, Hofheim, Germany) andwith their tips broken back under a microscope. .. These were filled with 10% neurobiotin (Vector Laboratories Inc., Burlingame, CA, USA) in 0.5 M potassium acetate containing 0.05% pyranine (Thermo Fisher, Kandel, Germany) to visualize the electrode tip (10 MΩ resistance).

    Glass:

    Article Title: Distinct roles of two thalamostriatal systems in visually triggered decision-making in common marmosets
    Article Snippet: 0.45 mm filter , KURABO , Cat# S-2504. .. Glass capillary , Sutter Instruments , Cat# B100-75-10. ..

    Concentration Assay:

    Article Title: Anti-VEGF antibody constructs
    Article Snippet: .. Introduction of rAAV particles was performed through the following steps: A) preauricular incision to expose the cochlear bulla, B) glass micropipettes (cat #4878—WPI) pulled with a micropipette puller (cat #P87—Sutter instruments) to a final OD of about 10 μm were used to manually deliver (micropipettes held by a Nanoliter 2000 micromanipulator—WPI) compositions containing rAAV particles into the scala tympanic, which allows access to inner ear cells, C) 1 μL of a composition described herein at a suitable concentration in suitable buffer (described in Example 1, rAAVAnc80-ranibizumab-PC particles at approximately 1.4×1010 vg/per cochlea, or rAAVAnc80-bevacizumab-PC particles at approximately 1.2×1010 vg/per cochlea; each diluted in in 1×PBS with pluronic acid F68) was injected into each tested cochlea at a release rate of 0.3 μL/min (controlled by MICRO4 microinjection controller—WPI). ..

    Injection:

    Article Title: Anti-VEGF antibody constructs
    Article Snippet: .. Introduction of rAAV particles was performed through the following steps: A) preauricular incision to expose the cochlear bulla, B) glass micropipettes (cat #4878—WPI) pulled with a micropipette puller (cat #P87—Sutter instruments) to a final OD of about 10 μm were used to manually deliver (micropipettes held by a Nanoliter 2000 micromanipulator—WPI) compositions containing rAAV particles into the scala tympanic, which allows access to inner ear cells, C) 1 μL of a composition described herein at a suitable concentration in suitable buffer (described in Example 1, rAAVAnc80-ranibizumab-PC particles at approximately 1.4×1010 vg/per cochlea, or rAAVAnc80-bevacizumab-PC particles at approximately 1.2×1010 vg/per cochlea; each diluted in in 1×PBS with pluronic acid F68) was injected into each tested cochlea at a release rate of 0.3 μL/min (controlled by MICRO4 microinjection controller—WPI). ..

    Microinjection:

    Article Title: Anti-VEGF antibody constructs
    Article Snippet: .. Introduction of rAAV particles was performed through the following steps: A) preauricular incision to expose the cochlear bulla, B) glass micropipettes (cat #4878—WPI) pulled with a micropipette puller (cat #P87—Sutter instruments) to a final OD of about 10 μm were used to manually deliver (micropipettes held by a Nanoliter 2000 micromanipulator—WPI) compositions containing rAAV particles into the scala tympanic, which allows access to inner ear cells, C) 1 μL of a composition described herein at a suitable concentration in suitable buffer (described in Example 1, rAAVAnc80-ranibizumab-PC particles at approximately 1.4×1010 vg/per cochlea, or rAAVAnc80-bevacizumab-PC particles at approximately 1.2×1010 vg/per cochlea; each diluted in in 1×PBS with pluronic acid F68) was injected into each tested cochlea at a release rate of 0.3 μL/min (controlled by MICRO4 microinjection controller—WPI). ..

    other:

    Article Title: Responses of inferior colliculus neurons to notched noises in awake mice: putative neural correlates of auditory enhancement and Zwicker tone
    Article Snippet: Electrodes were fabricated with a P-2000 horizontal micropipette puller (Sutter Instrument) and positioned using a precision digital micromanipulator (MP-225, Sutter Instrument) under a surgical microscope (Leica MZ9.5).

    Article Title: Alcohol disrupts long-term potentiation at hippocampus-medium spiny neuron synapses in the medial shell of the nucleus accumbens
    Article Snippet: Patch pipettes (4-8MΩ) were made from borosilicate glass (World Precision Instruments) using a Sutter Instruments P-97 model puller.

    Article Title: Highly sensitive and rapid screening of radical electrochemical intermediates by chemical labeling untargeted mass spectrometry.
    Article Snippet: Capturing and analyzing low-abundance, short-lived, and unstable radical intermediates during electrochemical (EC) reactions is important for understanding EC reaction mechanisms.. Herein, we developed a chemical labeling strategy for rapid screening and highly sensitive analysis of EC radical intermediates by mass spectrometry (MS).. Taking 5,5-dimethyl-1-pyrroline N-oxide (DMPO) labeling reagent as an example, DMPO can react with radical intermediates to form stable labeling products with characteristic fragmentation daughter ions or neutral loss in tandem MS, so the labeled intermediates can be screened by precursor ions scan or neutral loss scan.



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    Sutter Instrument Company borosilicate glass sharp electrodes p-2000 electrode puller
    Analysis of currents in canonical model (Fig. A) and in model with <t>\documentclass[12pt]{minimal}</t> \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}$$\end{document} I Ca -block. ( A ) Model fits for both full model (left) and blocked model (right) showing no systematic differences. ( B ) Current breakdown with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}\times $$\end{document} I Ca × 10 (dashed light blue line) showing a 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times $$\end{document} × magnified calcium current for illustrative purposes.
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    Image Search Results


    Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma

    doi: 10.3791/63463

    Figure Lengend Snippet: Materials

    Article Snippet: Borosilicate glass capillaries (Sharp Electrodes) , Warner Instruments , GC100F-10 , .

    Techniques: Dissection, Control, Fluorescence, Microscopy, Staining, Clinical Proteomics, Membrane, Isolation

    Analysis of currents in canonical model (Fig. A) and in model with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}$$\end{document} I Ca -block. ( A ) Model fits for both full model (left) and blocked model (right) showing no systematic differences. ( B ) Current breakdown with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}\times $$\end{document} I Ca × 10 (dashed light blue line) showing a 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times $$\end{document} × magnified calcium current for illustrative purposes.

    Journal: Scientific Reports

    Article Title: Dynamics of a neuronal pacemaker in the weakly electric fish Apteronotus

    doi: 10.1038/s41598-020-73566-3

    Figure Lengend Snippet: Analysis of currents in canonical model (Fig. A) and in model with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}$$\end{document} I Ca -block. ( A ) Model fits for both full model (left) and blocked model (right) showing no systematic differences. ( B ) Current breakdown with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}\times $$\end{document} I Ca × 10 (dashed light blue line) showing a 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times $$\end{document} × magnified calcium current for illustrative purposes.

    Article Snippet: After a minimum of 30 min, pacemaker recordings (intracellular and extracellular) were performed with borosilicate glass sharp electrodes (30–90 M \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\Omega $$\end{document} Ω , P-2000 electrode puller, Sutter Instrument Company, Novato, CA, USA) using an Axoclamp 2B amplifier (Molecular Devices, Sunnyvale, CA, USA).

    Techniques: Blocking Assay

    Data and model bifurcation analysis. ( A ) Relative time-series of pacemaker frequency as Na-free ACSF is washed in (see Methods) for 5 different pacemaker preparations. Green trace represents average (individual preparations in gray) and red trace represents cessation of firing. T = 1 represents bifurcation point. ( B ) Orbit diagram for model bifurcation analysis with respect to \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{E}_{\mathrm{Na}}$$\end{document} E Na . Green trace is action potential extrema. Black trace is unstable fixed point and red trace is stable fixed point. Black-Red intersection point is the Hopf bifurcation. ( C ) Frequency analysis of the model Hopf bifurcation. Dark green line represents model in Fig. C (i). Light green lines represent results from other model fits, Fig. C (ii–iv). Red line represents cessation and dotted lines show the bifurcation of each model.

    Journal: Scientific Reports

    Article Title: Dynamics of a neuronal pacemaker in the weakly electric fish Apteronotus

    doi: 10.1038/s41598-020-73566-3

    Figure Lengend Snippet: Data and model bifurcation analysis. ( A ) Relative time-series of pacemaker frequency as Na-free ACSF is washed in (see Methods) for 5 different pacemaker preparations. Green trace represents average (individual preparations in gray) and red trace represents cessation of firing. T = 1 represents bifurcation point. ( B ) Orbit diagram for model bifurcation analysis with respect to \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{E}_{\mathrm{Na}}$$\end{document} E Na . Green trace is action potential extrema. Black trace is unstable fixed point and red trace is stable fixed point. Black-Red intersection point is the Hopf bifurcation. ( C ) Frequency analysis of the model Hopf bifurcation. Dark green line represents model in Fig. C (i). Light green lines represent results from other model fits, Fig. C (ii–iv). Red line represents cessation and dotted lines show the bifurcation of each model.

    Article Snippet: After a minimum of 30 min, pacemaker recordings (intracellular and extracellular) were performed with borosilicate glass sharp electrodes (30–90 M \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\Omega $$\end{document} Ω , P-2000 electrode puller, Sutter Instrument Company, Novato, CA, USA) using an Axoclamp 2B amplifier (Molecular Devices, Sunnyvale, CA, USA).

    Techniques:

    Response to progressive block of Na \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + and K \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channels. ( A ) Model response to Na \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channel block ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{G}_{\mathrm{Na}}$$\end{document} G Na , left) and K \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channel block ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{G}_{\mathrm{K}}$$\end{document} G K , right). ( B ) Action potential properties computed as a function of block level for peak-peak amplitude (left), action potential rise rate (center) and fall rate (absolute value; right). Dots represent block level with equivalent percentage change in each property from data reported in .

    Journal: Scientific Reports

    Article Title: Dynamics of a neuronal pacemaker in the weakly electric fish Apteronotus

    doi: 10.1038/s41598-020-73566-3

    Figure Lengend Snippet: Response to progressive block of Na \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + and K \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channels. ( A ) Model response to Na \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channel block ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{G}_{\mathrm{Na}}$$\end{document} G Na , left) and K \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channel block ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{G}_{\mathrm{K}}$$\end{document} G K , right). ( B ) Action potential properties computed as a function of block level for peak-peak amplitude (left), action potential rise rate (center) and fall rate (absolute value; right). Dots represent block level with equivalent percentage change in each property from data reported in .

    Article Snippet: After a minimum of 30 min, pacemaker recordings (intracellular and extracellular) were performed with borosilicate glass sharp electrodes (30–90 M \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\Omega $$\end{document} Ω , P-2000 electrode puller, Sutter Instrument Company, Novato, CA, USA) using an Axoclamp 2B amplifier (Molecular Devices, Sunnyvale, CA, USA).

    Techniques: Blocking Assay