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sharp intracellular electrodes  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company sharp intracellular electrodes
    Sharp Intracellular Electrodes, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 98/100, based on 3829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sharp+borosilicate+electrodes/Borosilicate+Glass/pm39716055-151-11-31
    Average 98 stars, based on 3829 article reviews
    sharp intracellular electrodes - by Bioz Stars, 2026-10
    98/100 stars

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    other:

    Article Title: Distinct cell-type contributions and network topography of theta-nested gamma oscillations in the medial entorhinal cortex
    Article Snippet: Electrodes were pulled using a horizontal puller (Sutter Instrument) using filament, thin-wall borosilicate glass (Sutter Instrument).

    Article Title: A Single Dose of a Psychedelic Drug Repairs Prefrontal Cortex Synaptic Physiology in a Mouse Model of Prenatal Alcohol Exposure
    Article Snippet: Patch electrodes were pulled from borosilicate glass (Sutter Instruments) and had diameters of 2–4 μm and resistances of 3–5 MΩ.

    Article Title: Dual-peptide engineered macrophage membrane biomimetic nanosystem via targeting Rg1 delivery for traumatic brain injury therapy.
    Article Snippet: Borosilicate glass patch electrodes were fabricated using a Flaming-Brown puller (Sutter Instruments, CA) and filled with internal solution (in mM): 135 K-gluconate, 5 KCl, 10 HEPES, 2 EGTA, 4 ATP, 0.5 GTP (osmolality 280–290 mOsm, pH 7.4), yielding 4–7 MΩ resistance.

    Article Title: Mutant bacteriorhodopsin-like-channelrhodopsin ion channel
    Article Snippet: Patch pipettes with resistances of 2-6 MΩ were fabricated from thin-walled borosilicate glass on a horizontal puller (Model P-1000, Sutter Instruments, Novato, USA).

    Article Title: Targeting G i/o -coupled GPCRs to inhibit nociceptors: insights from the serotonin receptor Htr1b and triptans
    Article Snippet: Patch pipettes (5–7 MΩ) were pulled from borosilicate glass (Sutter Instruments) and filled with an internal solution containing: 135 mM CsMeSO 3 , 4 mM ATP-Mg 2+ , 0.3 mM GTP-Na + , 1 mM EGTA, 3.3 mM QX-314(Cl - salt), 8 mM Na 2 -phoshocreatine and 10 mM HEPES.

    Article Title: Small molecule inhibitors of calcium channel activity and uses thereof
    Article Snippet: Substantia gelatinosa neurons were visualized and identified in the slices by means of infrared differential interference contrast video microscopy on an upright microscope (FN1; Nikon, Tokyo, Japan) equipped with a 3 40/0.80 water-immersion objective and a charge-coupled device camera.

    Article Title: Non-vectorial integration of intersectional short-pulse stimulation enables enhanced deep brain modulation and effective seizure control.
    Article Snippet: Pipettes were pulled from thick-walled borosilicate glass (BF150-86-15, Sutter Instrument, Novato, CA) with a Sutter P-1000 puller (Sutter Instrument, Novato, CA).

    Transferring:

    Article Title: Neuronal Igfbp2 deficiency in the prefrontal cortex impairs cognition through synaptic dysfunction in male mice
    Article Snippet: Neurons in the PFC were visualized using infrared differential interference contrast (IR-DIC) video microscopy with a 40× water-immersion objective (BX51WI, Olympus, Japan). .. Patch pipettes with resistances of 3–5 MΩ were fabricated from borosilicate glass capillaries using a horizontal pipette puller (P-1000, Sutter Instrument, USA). ..



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    Sutter Instrument Company borosilicate glass sharp electrodes p-2000 electrode puller
    Analysis of currents in canonical model (Fig. A) and in model with <t>\documentclass[12pt]{minimal}</t> \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}$$\end{document} I Ca -block. ( A ) Model fits for both full model (left) and blocked model (right) showing no systematic differences. ( B ) Current breakdown with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}\times $$\end{document} I Ca × 10 (dashed light blue line) showing a 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times $$\end{document} × magnified calcium current for illustrative purposes.
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    Image Search Results


    Materials

    Journal: Journal of visualized experiments : JoVE

    Article Title: Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma

    doi: 10.3791/63463

    Figure Lengend Snippet: Materials

    Article Snippet: Borosilicate glass capillaries (Sharp Electrodes) , Warner Instruments , GC100F-10 , .

    Techniques: Dissection, Control, Fluorescence, Microscopy, Staining, Clinical Proteomics, Membrane, Isolation

    Analysis of currents in canonical model (Fig. A) and in model with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}$$\end{document} I Ca -block. ( A ) Model fits for both full model (left) and blocked model (right) showing no systematic differences. ( B ) Current breakdown with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}\times $$\end{document} I Ca × 10 (dashed light blue line) showing a 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times $$\end{document} × magnified calcium current for illustrative purposes.

    Journal: Scientific Reports

    Article Title: Dynamics of a neuronal pacemaker in the weakly electric fish Apteronotus

    doi: 10.1038/s41598-020-73566-3

    Figure Lengend Snippet: Analysis of currents in canonical model (Fig. A) and in model with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}$$\end{document} I Ca -block. ( A ) Model fits for both full model (left) and blocked model (right) showing no systematic differences. ( B ) Current breakdown with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {I}_{\mathrm{Ca}}\times $$\end{document} I Ca × 10 (dashed light blue line) showing a 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times $$\end{document} × magnified calcium current for illustrative purposes.

    Article Snippet: After a minimum of 30 min, pacemaker recordings (intracellular and extracellular) were performed with borosilicate glass sharp electrodes (30–90 M \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\Omega $$\end{document} Ω , P-2000 electrode puller, Sutter Instrument Company, Novato, CA, USA) using an Axoclamp 2B amplifier (Molecular Devices, Sunnyvale, CA, USA).

    Techniques: Blocking Assay

    Data and model bifurcation analysis. ( A ) Relative time-series of pacemaker frequency as Na-free ACSF is washed in (see Methods) for 5 different pacemaker preparations. Green trace represents average (individual preparations in gray) and red trace represents cessation of firing. T = 1 represents bifurcation point. ( B ) Orbit diagram for model bifurcation analysis with respect to \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{E}_{\mathrm{Na}}$$\end{document} E Na . Green trace is action potential extrema. Black trace is unstable fixed point and red trace is stable fixed point. Black-Red intersection point is the Hopf bifurcation. ( C ) Frequency analysis of the model Hopf bifurcation. Dark green line represents model in Fig. C (i). Light green lines represent results from other model fits, Fig. C (ii–iv). Red line represents cessation and dotted lines show the bifurcation of each model.

    Journal: Scientific Reports

    Article Title: Dynamics of a neuronal pacemaker in the weakly electric fish Apteronotus

    doi: 10.1038/s41598-020-73566-3

    Figure Lengend Snippet: Data and model bifurcation analysis. ( A ) Relative time-series of pacemaker frequency as Na-free ACSF is washed in (see Methods) for 5 different pacemaker preparations. Green trace represents average (individual preparations in gray) and red trace represents cessation of firing. T = 1 represents bifurcation point. ( B ) Orbit diagram for model bifurcation analysis with respect to \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{E}_{\mathrm{Na}}$$\end{document} E Na . Green trace is action potential extrema. Black trace is unstable fixed point and red trace is stable fixed point. Black-Red intersection point is the Hopf bifurcation. ( C ) Frequency analysis of the model Hopf bifurcation. Dark green line represents model in Fig. C (i). Light green lines represent results from other model fits, Fig. C (ii–iv). Red line represents cessation and dotted lines show the bifurcation of each model.

    Article Snippet: After a minimum of 30 min, pacemaker recordings (intracellular and extracellular) were performed with borosilicate glass sharp electrodes (30–90 M \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\Omega $$\end{document} Ω , P-2000 electrode puller, Sutter Instrument Company, Novato, CA, USA) using an Axoclamp 2B amplifier (Molecular Devices, Sunnyvale, CA, USA).

    Techniques:

    Response to progressive block of Na \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + and K \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channels. ( A ) Model response to Na \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channel block ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{G}_{\mathrm{Na}}$$\end{document} G Na , left) and K \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channel block ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{G}_{\mathrm{K}}$$\end{document} G K , right). ( B ) Action potential properties computed as a function of block level for peak-peak amplitude (left), action potential rise rate (center) and fall rate (absolute value; right). Dots represent block level with equivalent percentage change in each property from data reported in .

    Journal: Scientific Reports

    Article Title: Dynamics of a neuronal pacemaker in the weakly electric fish Apteronotus

    doi: 10.1038/s41598-020-73566-3

    Figure Lengend Snippet: Response to progressive block of Na \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + and K \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channels. ( A ) Model response to Na \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channel block ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{G}_{\mathrm{Na}}$$\end{document} G Na , left) and K \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^+$$\end{document} + channel block ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mathrm{G}_{\mathrm{K}}$$\end{document} G K , right). ( B ) Action potential properties computed as a function of block level for peak-peak amplitude (left), action potential rise rate (center) and fall rate (absolute value; right). Dots represent block level with equivalent percentage change in each property from data reported in .

    Article Snippet: After a minimum of 30 min, pacemaker recordings (intracellular and extracellular) were performed with borosilicate glass sharp electrodes (30–90 M \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\Omega $$\end{document} Ω , P-2000 electrode puller, Sutter Instrument Company, Novato, CA, USA) using an Axoclamp 2B amplifier (Molecular Devices, Sunnyvale, CA, USA).

    Techniques: Blocking Assay