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sequest-ht algorithm  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sequest-ht algorithm
    Sequest Ht Algorithm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequest+ht+algorithm/sequestht+algorithm/pm37289994__bc3c00185_si_001-249-0-2
    Average 90 stars, based on 1 article reviews
    sequest-ht algorithm - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Mass Spectrometry-Based Proteomics in Clinical Diagnosis of Amyloidosis and Multiple Myeloma: A Review (2012-2024).
    Article Snippet: SEQUEST [105], one of the first MS/MS algorithms, is still widely used, especially when integrated with Thermo Fisher Scientific systems.

    Article Title: Live-Cell Mitochondrial Targeted NIR Fluorescent Covalent Labeling of Specific Proteins Using a Dual Localization Effect.
    Article Snippet: SEQUEST-HT algorithm (Thermo Fisher Scientific) was used to do the searches against the Uniprot database (taxonomy id: 9606 and keyword: KW-0496; downloaded on February 1, 2023).

    Article Title: Characterisation of extracellular vesicles in baculovirus infection of Spodoptera frugiperda cells
    Article Snippet: The MS and MS/MS scans were searched against Uniprot database using Protein Discovery 2.2 software, Sequest HT algorithm (Thermo Fisher Scientific).

    Article Title: Mass Spectrometry–Based Proteomics in Clinical Diagnosis of Amyloidosis and Multiple Myeloma: A Review (2012–2024)
    Article Snippet: SEQUEST [ ], one of the first MS/MS algorithms, is still widely used, especially when integrated with Thermo Fisher Scientific systems.

    Tandem Mass Spectroscopy:

    Article Title: Mycelial nutrient transfer promotes bacterial co-metabolic organochlorine pesticide degradation in nutrient-deprived environments
    Article Snippet: .. Proteome Discoverer (v1.4.1.14, Thermo Scientific) was used for protein identification and the acquired MS/MS spectra were searched with the SequestHT algorithm against the UniProt reference proteome of Sphingobium sp. and Fusarium equiseti. ..

    Mass Spectrometry:

    Article Title: Decellularized Extracellular Matrix Powder Accelerates Metabolic Maturation at Early Stages of Cardiac Differentiation in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
    Article Snippet: During fetal development, cardiomyocytes switch from glycolysis to oxidative metabolism to sustain the energy requirements of functional cells.. State-of-the-art cardiac differentiation protocols yield phenotypically immature cardiomyocytes, and common methods to improve metabolic maturation require multistep protocols to induce maturation only after cardiac specification is completed.. Here, we describe a maturation method using ventricle-derived decellularized extracellular matrix (dECM) that promoted earlystage metabolic maturation of cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs).

    Software:

    Article Title: Targeting of intracellular oncoproteins with peptide-centric CARs
    Article Snippet: .. Data were processed against the human proteome per the Swiss-Prot database ( https://www.uniprot.org , release 27 May 2021; 20,395 reviewed protein sequences contained) using the SequestHT algorithm in Proteome Discoverer (v.2.1; Thermo Fisher Scientific) software. ..

    Article Title: Unveiling the levels and significance of different serpin family proteins in aqueous humor dynamics.
    Article Snippet: .. To quantify and identify the proteins, raw mass spectrometry (MS) data were processed using Proteome Discoverer software (v1.4; Thermo Fisher Scientific) and searched against the Uniprot-SwissProt human database, which contains 20,385 entries, using the SequestHT algorithm. ..



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    Image Search Results


    Journal: iScience

    Article Title: Bromodomain protein BRD8 regulates cell cycle progression in colorectal cancer cells through a TIP60-independent regulation of the pre-RC complex

    doi: 10.1016/j.isci.2023.106563

    Figure Lengend Snippet:

    Article Snippet: Peptide identification was performed using SEQUEST HT algorithm in Proteome Discoverer Software (v2.5) (Thermo Fisher Scientific) based on the statistical criterion of a false discovery rate <1%.

    Techniques: Recombinant, Plasmid Preparation, Cell Cycle Assay, CCK-8 Assay, Flow Cytometry, Viability Assay, Ubiquitin Proteomics, Expressing, Cloning, Mutagenesis, Software