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anti atf6  (Proteintech)


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    Proteintech anti atf6
    ( A ) Schematic representation of IRE1 with a C-terminal HaloTag, the construct used for tagging IRE1 at the endogenous locus. IF1 L and IF2 L refer to the primary dimerization and oligomerization interfaces of the lumenal domain, respectively. ( B ) RT-PCR analysis of stress-dependent XBP1 mRNA splicing in WT U-2 OS cells, IRE1 knock-out (KO) U-2 OS cells, and U-2 OS cells in which IRE1 has been fully edited with a C-terminal HaloTag. Tm indicates treatment with 5 μg/ml tunicamycin. ( C ) Immunoblot of UPR activation in response to 5 μg /ml tunicamycin (left) and 100 nM thapsigargin (right) treatments in the three cell lines shown in panel B. ( D ) Maximum intensity projections of representative spinning-disk confocal images of live cells expressing endogenously tagged IRE1-HaloTag, labeled with the JF549 dye. Regions shown with yellow boxes are enlarged below. ( E ) Same as D, except the cells have been treated with 5 μg/ml tunicamycin for 5 hr. Figure 1—source data 1. Annotated uncropped gel used to generate . Figure 1—source data 2. Raw uncropped gel used to generate . Figure 1—source data 3. All annotated uncropped gels used to generate . Figure 1—source data 4. Raw uncropped gel of immunoblot against IRE1 and phospho-IRE1 in . Figure 1—source data 5. Raw uncropped gel of immunoblot against XBP1 in . Figure 1—source data 6. Raw uncropped gel of immunoblot against PERK and actin in . Figure 1—source data 7. Raw uncropped gel of immunoblot against ATF4 in . Figure 1—source data 8. Raw uncropped gel of immunoblot against <t>ATF6</t> in . Figure 1—source data 9. Raw uncropped gel of immunoblot against CHOP in .
    Anti Atf6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing-based+spatial+transcriptomics+data/pmc09217129-23-2-7?v=Proteintech
    Average 96 stars, based on 413 article reviews
    anti atf6 - by Bioz Stars, 2026-07
    96/100 stars

    Images

    1) Product Images from "Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers"

    Article Title: Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers

    Journal: eLife

    doi: 10.7554/eLife.74342

    ( A ) Schematic representation of IRE1 with a C-terminal HaloTag, the construct used for tagging IRE1 at the endogenous locus. IF1 L and IF2 L refer to the primary dimerization and oligomerization interfaces of the lumenal domain, respectively. ( B ) RT-PCR analysis of stress-dependent XBP1 mRNA splicing in WT U-2 OS cells, IRE1 knock-out (KO) U-2 OS cells, and U-2 OS cells in which IRE1 has been fully edited with a C-terminal HaloTag. Tm indicates treatment with 5 μg/ml tunicamycin. ( C ) Immunoblot of UPR activation in response to 5 μg /ml tunicamycin (left) and 100 nM thapsigargin (right) treatments in the three cell lines shown in panel B. ( D ) Maximum intensity projections of representative spinning-disk confocal images of live cells expressing endogenously tagged IRE1-HaloTag, labeled with the JF549 dye. Regions shown with yellow boxes are enlarged below. ( E ) Same as D, except the cells have been treated with 5 μg/ml tunicamycin for 5 hr. Figure 1—source data 1. Annotated uncropped gel used to generate . Figure 1—source data 2. Raw uncropped gel used to generate . Figure 1—source data 3. All annotated uncropped gels used to generate . Figure 1—source data 4. Raw uncropped gel of immunoblot against IRE1 and phospho-IRE1 in . Figure 1—source data 5. Raw uncropped gel of immunoblot against XBP1 in . Figure 1—source data 6. Raw uncropped gel of immunoblot against PERK and actin in . Figure 1—source data 7. Raw uncropped gel of immunoblot against ATF4 in . Figure 1—source data 8. Raw uncropped gel of immunoblot against ATF6 in . Figure 1—source data 9. Raw uncropped gel of immunoblot against CHOP in .
    Figure Legend Snippet: ( A ) Schematic representation of IRE1 with a C-terminal HaloTag, the construct used for tagging IRE1 at the endogenous locus. IF1 L and IF2 L refer to the primary dimerization and oligomerization interfaces of the lumenal domain, respectively. ( B ) RT-PCR analysis of stress-dependent XBP1 mRNA splicing in WT U-2 OS cells, IRE1 knock-out (KO) U-2 OS cells, and U-2 OS cells in which IRE1 has been fully edited with a C-terminal HaloTag. Tm indicates treatment with 5 μg/ml tunicamycin. ( C ) Immunoblot of UPR activation in response to 5 μg /ml tunicamycin (left) and 100 nM thapsigargin (right) treatments in the three cell lines shown in panel B. ( D ) Maximum intensity projections of representative spinning-disk confocal images of live cells expressing endogenously tagged IRE1-HaloTag, labeled with the JF549 dye. Regions shown with yellow boxes are enlarged below. ( E ) Same as D, except the cells have been treated with 5 μg/ml tunicamycin for 5 hr. Figure 1—source data 1. Annotated uncropped gel used to generate . Figure 1—source data 2. Raw uncropped gel used to generate . Figure 1—source data 3. All annotated uncropped gels used to generate . Figure 1—source data 4. Raw uncropped gel of immunoblot against IRE1 and phospho-IRE1 in . Figure 1—source data 5. Raw uncropped gel of immunoblot against XBP1 in . Figure 1—source data 6. Raw uncropped gel of immunoblot against PERK and actin in . Figure 1—source data 7. Raw uncropped gel of immunoblot against ATF4 in . Figure 1—source data 8. Raw uncropped gel of immunoblot against ATF6 in . Figure 1—source data 9. Raw uncropped gel of immunoblot against CHOP in .

    Techniques Used: Construct, Reverse Transcription Polymerase Chain Reaction, Knock-Out, Western Blot, Activation Assay, Expressing, Labeling

    ( A ) Immunoblot showing IRE1 expression levels and UPR activation in WT U-2 OS cells, IRE1 KO U-2 OS cells, partial KO cells used as the parental cell line for generating HaloTag knock-ins, and two clones of endogenously labeled HaloTag (with high and low IRE1 expression levels). Note the shift in protein size due to the addition of the HaloTag and the absence of a WT IRE1 band in the two clones on the right. ( B ) Flow cytometry analysis of the low-and high-expressing clones shown in panel A. Cells were labeled with 5 nM JF549-HaloTag dye for 1 hr prior to the start of the flow cytometry experiment. Note the unimodal intensity distributions of both clones, ruling out the possibility that the lower-expressing clone simply contains a bimodal mixture of low- and high-expressing cells. Error bars represent 95% confidence intervals. Figure 1—figure supplement 1—source data 1. Annotated uncropped gel used to generate . Figure 1—figure supplement 1—source data 2. Raw uncropped gel of immunoblot against IRE1 and phospho-IRE1 in . Figure 1—figure supplement 1—source data 3. Raw uncropped gel of immunoblot against XBP1 in . Figure 1—figure supplement 1—source data 4. Raw uncropped gel of immunoblot against PERK in . Figure 1—figure supplement 1—source data 5. Raw uncropped gel of immunoblot against ATF4 and CHOP in . Figure 1—figure supplement 1—source data 6. Raw uncropped gel of immunoblot against ATF6 in . Figure 1—figure supplement 1—source data 7. Raw uncropped gel of immunoblot against actin in .
    Figure Legend Snippet: ( A ) Immunoblot showing IRE1 expression levels and UPR activation in WT U-2 OS cells, IRE1 KO U-2 OS cells, partial KO cells used as the parental cell line for generating HaloTag knock-ins, and two clones of endogenously labeled HaloTag (with high and low IRE1 expression levels). Note the shift in protein size due to the addition of the HaloTag and the absence of a WT IRE1 band in the two clones on the right. ( B ) Flow cytometry analysis of the low-and high-expressing clones shown in panel A. Cells were labeled with 5 nM JF549-HaloTag dye for 1 hr prior to the start of the flow cytometry experiment. Note the unimodal intensity distributions of both clones, ruling out the possibility that the lower-expressing clone simply contains a bimodal mixture of low- and high-expressing cells. Error bars represent 95% confidence intervals. Figure 1—figure supplement 1—source data 1. Annotated uncropped gel used to generate . Figure 1—figure supplement 1—source data 2. Raw uncropped gel of immunoblot against IRE1 and phospho-IRE1 in . Figure 1—figure supplement 1—source data 3. Raw uncropped gel of immunoblot against XBP1 in . Figure 1—figure supplement 1—source data 4. Raw uncropped gel of immunoblot against PERK in . Figure 1—figure supplement 1—source data 5. Raw uncropped gel of immunoblot against ATF4 and CHOP in . Figure 1—figure supplement 1—source data 6. Raw uncropped gel of immunoblot against ATF6 in . Figure 1—figure supplement 1—source data 7. Raw uncropped gel of immunoblot against actin in .

    Techniques Used: Western Blot, Expressing, Activation Assay, Clone Assay, Labeling, Flow Cytometry

    Single-particle tracking data showing stress-dependent oligomerization of the high- and low-expressing IRE1-HaloTag clones. IRE1 in the lower-expressing clone remains dimeric in unstressed cells, while the shift to higher-order oligomers upon stress is less prominent than in the higher-expressing clone. Each data point represents a single cell. Error bars represent 95% confidence intervals. Figure 4—figure supplement 1—source data 1. Raw uncropped gel of immunoblot against IRE1, XBP1s, and GAPDH of . Figure 4—figure supplement 1—source data 2. Raw uncropped gel of immunoblot against PERK, ATF4, and CHOP of . Figure 4—figure supplement 1—source data 3. Raw uncropped gel of immunoblot against ATF6 of .
    Figure Legend Snippet: Single-particle tracking data showing stress-dependent oligomerization of the high- and low-expressing IRE1-HaloTag clones. IRE1 in the lower-expressing clone remains dimeric in unstressed cells, while the shift to higher-order oligomers upon stress is less prominent than in the higher-expressing clone. Each data point represents a single cell. Error bars represent 95% confidence intervals. Figure 4—figure supplement 1—source data 1. Raw uncropped gel of immunoblot against IRE1, XBP1s, and GAPDH of . Figure 4—figure supplement 1—source data 2. Raw uncropped gel of immunoblot against PERK, ATF4, and CHOP of . Figure 4—figure supplement 1—source data 3. Raw uncropped gel of immunoblot against ATF6 of .

    Techniques Used: Single-particle Tracking, Expressing, Clone Assay, Western Blot


    Figure Legend Snippet:

    Techniques Used: Cloning, CRISPR, Knock-Out, Expressing, Recombinant, Plasmid Preparation, Transfection, Sequencing, Software, Diffusion-based Assay, Single Particle



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