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anti cnga2 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti cnga2 antibody
    A, the increase in the dye diffusion distance induced by 2‐PAA (20 μm, 1 h) was not blocked by the protein kinase A inhibitors Rp‐cAMPS (Rp, 200 μm) and KT5720 (KT, 1 μm). Additionally, the activator of the exchange protein directly activated by cAMP, 8‐pCPT‐O‐Me‐cAMP (100 μm) did not affect the dye diffusion distance. B, the cyclic nucleotide‐gated (CNG) channel inhibitor l‐cis‐diltiazem (L‐cis‐dil., 100 μm) could prevent the increase in the dye diffusion distance induced by 2‐PAA (20 μm, 1 h) relative to the vehicle control (cont., 0.3% ethanol) in scrape loading/dye transfer assays. C, RT‐PCR showed that the CNG channel subunits A1, A2 and B1 were expressed in hCMEC/D3 cells. D, furthermore, the cAMP‐sensitive subunit <t>CNGA2</t> was confirmed to be expressed at protein level with β‐tubulin (β‐Tb) serving as loading control. E, in whole‐cell patch‐clamp experiments, 2‐PAA (20 μm) increased the current measured in hCMEC/D3 cells. This increased current was completely abolished by simultaneous application of the CNG channel blocker l‐cis‐diltiazem (L‐cis‐dil., 100 μm) with 2‐PAA. All results were analysed using Student's t test. *Significant differences to the vehicle control: * P < 0.05, ** P < 0.01, *** P < 0.001; #significant differences to 2‐PAA: # P < 0.05.
    Anti Cnga2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing-based+spatial+transcriptomics+data/Anti-Connexin-37+Antibody/pmc05390872-210-11-13
    Average 92 stars, based on 1 article reviews
    anti cnga2 antibody - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Adenosine receptors regulate gap junction coupling of the human cerebral microvascular endothelial cells hCMEC/D3 by Ca 2+ influx through cyclic nucleotide‐gated channels"

    Article Title: Adenosine receptors regulate gap junction coupling of the human cerebral microvascular endothelial cells hCMEC/D3 by Ca 2+ influx through cyclic nucleotide‐gated channels

    Journal: The Journal of Physiology

    doi: 10.1113/JP273150

    A, the increase in the dye diffusion distance induced by 2‐PAA (20 μm, 1 h) was not blocked by the protein kinase A inhibitors Rp‐cAMPS (Rp, 200 μm) and KT5720 (KT, 1 μm). Additionally, the activator of the exchange protein directly activated by cAMP, 8‐pCPT‐O‐Me‐cAMP (100 μm) did not affect the dye diffusion distance. B, the cyclic nucleotide‐gated (CNG) channel inhibitor l‐cis‐diltiazem (L‐cis‐dil., 100 μm) could prevent the increase in the dye diffusion distance induced by 2‐PAA (20 μm, 1 h) relative to the vehicle control (cont., 0.3% ethanol) in scrape loading/dye transfer assays. C, RT‐PCR showed that the CNG channel subunits A1, A2 and B1 were expressed in hCMEC/D3 cells. D, furthermore, the cAMP‐sensitive subunit CNGA2 was confirmed to be expressed at protein level with β‐tubulin (β‐Tb) serving as loading control. E, in whole‐cell patch‐clamp experiments, 2‐PAA (20 μm) increased the current measured in hCMEC/D3 cells. This increased current was completely abolished by simultaneous application of the CNG channel blocker l‐cis‐diltiazem (L‐cis‐dil., 100 μm) with 2‐PAA. All results were analysed using Student's t test. *Significant differences to the vehicle control: * P < 0.05, ** P < 0.01, *** P < 0.001; #significant differences to 2‐PAA: # P < 0.05.
    Figure Legend Snippet: A, the increase in the dye diffusion distance induced by 2‐PAA (20 μm, 1 h) was not blocked by the protein kinase A inhibitors Rp‐cAMPS (Rp, 200 μm) and KT5720 (KT, 1 μm). Additionally, the activator of the exchange protein directly activated by cAMP, 8‐pCPT‐O‐Me‐cAMP (100 μm) did not affect the dye diffusion distance. B, the cyclic nucleotide‐gated (CNG) channel inhibitor l‐cis‐diltiazem (L‐cis‐dil., 100 μm) could prevent the increase in the dye diffusion distance induced by 2‐PAA (20 μm, 1 h) relative to the vehicle control (cont., 0.3% ethanol) in scrape loading/dye transfer assays. C, RT‐PCR showed that the CNG channel subunits A1, A2 and B1 were expressed in hCMEC/D3 cells. D, furthermore, the cAMP‐sensitive subunit CNGA2 was confirmed to be expressed at protein level with β‐tubulin (β‐Tb) serving as loading control. E, in whole‐cell patch‐clamp experiments, 2‐PAA (20 μm) increased the current measured in hCMEC/D3 cells. This increased current was completely abolished by simultaneous application of the CNG channel blocker l‐cis‐diltiazem (L‐cis‐dil., 100 μm) with 2‐PAA. All results were analysed using Student's t test. *Significant differences to the vehicle control: * P < 0.05, ** P < 0.01, *** P < 0.001; #significant differences to 2‐PAA: # P < 0.05.

    Techniques Used: Diffusion-based Assay, Reverse Transcription Polymerase Chain Reaction, Patch Clamp

    List of all primer pairs used for gene expression analyses and quantitative real time PCR
    Figure Legend Snippet: List of all primer pairs used for gene expression analyses and quantitative real time PCR

    Techniques Used: Expressing, Sequencing, Amplification

    Related Articles

    Recombinant:

    Article Title: Aging-induced changes in lymphatic muscle cell transcriptomes are associated with reduced pumping of peripheral collecting lymphatic vessels in mice.
    Article Snippet: Resource Aging-induced changes in lymphatic muscle cell transcriptomes are associated with reduced pumping of peripheral collecting lymphatic vessels in mice

    Article Title:
    Article Snippet: .. All primary antibodies are listed in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Molecular marker Immunogen Host Dilution Clone Source RRID Albumin whole mouse albumin goat 1:800 poly Bethyl, A90–234A RRID:AB_67122 claudin5 clone 4C3C2, Alexa488 Synthetic peptide from the mouse claudin5 mouse 1:10000 mono Invitrogen, 352588 RRID:AB_2532189 Connexin 43 (Cx43s) PSSRASSRASSRPRPDDLEI rabbit 1:2000 poly Sigma, C6219 RRID:AB_476857 Connexin 43 (Cx43a) (C)HAQPFDFPDDNQNSK rabbit 1:10000 poly Alomone, ACC-201 RRID:AB_10917597 Connexin 40 (C)GHRFPQGYHSDKR rabbit 1:3000 poly Alomone, ACC-205 RRID:AB_2340916 Connexin 37 (C)EHQMAKISVAEDGR rabbit 1:2000 poly Alomone, ACC-204 RRID:AB_2722598 Smooth muscle actin, SMA, 1A4 N-terminal synthetic decapeptide of α-SMA mouse 1:1000 mono Sigma, A5228 RRID:AB_262054 Glial fibrillary acidic protein, GFAP purified bovine GFAP chicken 1:3000 poly Millipore, AB5541 RRID:AB_177521 CD31/PECAM-1 Mouse myeloma cell NS0-derived recombinant mouse CD31 Glu18-Lys590 goat 1:6000 poly RD, AF3628 RRID:AB_2161028 Open in a separate window Primary antibodies Albumin antibody. ..

    Article Title:
    Article Snippet: All electroporations were made with MultiClamp 700B patch-clamp amplifier (Molecular Devices, Sunnyvale, CA) using Signal software (CED, UK). .. All primary antibodies are listed in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Molecular marker Immunogen Host Dilution Clone Source RRID Albumin whole mouse albumin goat 1:800 poly Bethyl, A90–234A RRID:AB_67122 claudin5 clone 4C3C2, Alexa488 Synthetic peptide from the mouse claudin5 mouse 1:10000 mono Invitrogen, 352588 RRID:AB_2532189 Connexin 43 (Cx43s) PSSRASSRASSRPRPDDLEI rabbit 1:2000 poly Sigma, C6219 RRID:AB_476857 Connexin 43 (Cx43a) (C)HAQPFDFPDDNQNSK rabbit 1:10000 poly Alomone, ACC-201 RRID:AB_10917597 Connexin 40 (C)GHRFPQGYHSDKR rabbit 1:3000 poly Alomone, ACC-205 RRID:AB_2340916 Connexin 37 (C)EHQMAKISVAEDGR rabbit 1:2000 poly Alomone, ACC-204 RRID:AB_2722598 Smooth muscle actin, SMA, 1A4 N-terminal synthetic decapeptide of α-SMA mouse 1:1000 mono Sigma, A5228 RRID:AB_262054 Glial fibrillary acidic protein, GFAP purified bovine GFAP chicken 1:3000 poly Millipore, AB5541 RRID:AB_177521 CD31/PECAM-1 Mouse myeloma cell NS0-derived recombinant mouse CD31 Glu18-Lys590 goat 1:6000 poly RD, AF3628 RRID:AB_2161028 Open in a separate window Primary antibodies ..

    Marker:

    Article Title:
    Article Snippet: .. All primary antibodies are listed in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Molecular marker Immunogen Host Dilution Clone Source RRID Albumin whole mouse albumin goat 1:800 poly Bethyl, A90–234A RRID:AB_67122 claudin5 clone 4C3C2, Alexa488 Synthetic peptide from the mouse claudin5 mouse 1:10000 mono Invitrogen, 352588 RRID:AB_2532189 Connexin 43 (Cx43s) PSSRASSRASSRPRPDDLEI rabbit 1:2000 poly Sigma, C6219 RRID:AB_476857 Connexin 43 (Cx43a) (C)HAQPFDFPDDNQNSK rabbit 1:10000 poly Alomone, ACC-201 RRID:AB_10917597 Connexin 40 (C)GHRFPQGYHSDKR rabbit 1:3000 poly Alomone, ACC-205 RRID:AB_2340916 Connexin 37 (C)EHQMAKISVAEDGR rabbit 1:2000 poly Alomone, ACC-204 RRID:AB_2722598 Smooth muscle actin, SMA, 1A4 N-terminal synthetic decapeptide of α-SMA mouse 1:1000 mono Sigma, A5228 RRID:AB_262054 Glial fibrillary acidic protein, GFAP purified bovine GFAP chicken 1:3000 poly Millipore, AB5541 RRID:AB_177521 CD31/PECAM-1 Mouse myeloma cell NS0-derived recombinant mouse CD31 Glu18-Lys590 goat 1:6000 poly RD, AF3628 RRID:AB_2161028 Open in a separate window Primary antibodies Albumin antibody. ..

    Article Title:
    Article Snippet: All electroporations were made with MultiClamp 700B patch-clamp amplifier (Molecular Devices, Sunnyvale, CA) using Signal software (CED, UK). .. All primary antibodies are listed in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Molecular marker Immunogen Host Dilution Clone Source RRID Albumin whole mouse albumin goat 1:800 poly Bethyl, A90–234A RRID:AB_67122 claudin5 clone 4C3C2, Alexa488 Synthetic peptide from the mouse claudin5 mouse 1:10000 mono Invitrogen, 352588 RRID:AB_2532189 Connexin 43 (Cx43s) PSSRASSRASSRPRPDDLEI rabbit 1:2000 poly Sigma, C6219 RRID:AB_476857 Connexin 43 (Cx43a) (C)HAQPFDFPDDNQNSK rabbit 1:10000 poly Alomone, ACC-201 RRID:AB_10917597 Connexin 40 (C)GHRFPQGYHSDKR rabbit 1:3000 poly Alomone, ACC-205 RRID:AB_2340916 Connexin 37 (C)EHQMAKISVAEDGR rabbit 1:2000 poly Alomone, ACC-204 RRID:AB_2722598 Smooth muscle actin, SMA, 1A4 N-terminal synthetic decapeptide of α-SMA mouse 1:1000 mono Sigma, A5228 RRID:AB_262054 Glial fibrillary acidic protein, GFAP purified bovine GFAP chicken 1:3000 poly Millipore, AB5541 RRID:AB_177521 CD31/PECAM-1 Mouse myeloma cell NS0-derived recombinant mouse CD31 Glu18-Lys590 goat 1:6000 poly RD, AF3628 RRID:AB_2161028 Open in a separate window Primary antibodies ..

    Purification:

    Article Title:
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    Article Title:
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    Staining:

    Article Title: Aging-induced changes in lymphatic muscle cell transcriptomes are associated with reduced pumping of peripheral collecting lymphatic vessels in mice.
    Article Snippet: Resource Aging-induced changes in lymphatic muscle cell transcriptomes are associated with reduced pumping of peripheral collecting lymphatic vessels in mice

    other:

    Article Title:
    Article Snippet: Connexin 37 , (C)EHQMAKISVAEDGR , rabbit , 1:2000 , poly , Alomone, ACC-204 , RRID:AB_2722598.



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