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next-generation sequencing (ngs) of cdna libraries  (Oxford Nanopore)

 
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    Structured Review

    Oxford Nanopore next-generation sequencing (ngs) of cdna libraries
    Next Generation Sequencing (Ngs) Of Cdna Libraries, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+methods+nextgen+sequencing+methods/ngs/pmc07774889-63-5-17
    Average 90 stars, based on 1 article reviews
    next-generation sequencing (ngs) of cdna libraries - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: eLaRodON: identification of large genomic rearrangements in Oxford Nanopore sequencing data
    Article Snippet: A.T. and M.F. provided samples for sequencing DNA with Oxford Nanopore technology. .. A.K. and V.B. prepared NGS libraries for Oxford Nanopore sequencing. ..

    Article Title: Feasibility of long-read sequencing to identify molecular alterations in an Indonesian cohort of locally advanced to advanced nasopharyngeal cancer.
    Article Snippet: .. Primarily due to the availability long read sequencers, for example NGS from Oxford Nanopore or PacBio, which can better capture major SVs5. ..

    Article Title: Egypt Genome: Towards an African new genomic era
    Article Snippet: Several copies of various automated equipment were purchased and installed in the lab, including instruments for DNA extraction (chemagicTM 360, Perkin Elmer, Waltham, MA, USA), DNA quality assurance (Qubit 4 Fluorometer (Invitrogen, Carlsbad, CA, USA), NanoDropTM One (Thermo Scientific, Waltham, MA, USA) andLabChip GX Touch (Perkin Elmer)), NGS library preparation (ultrasonicator M220 (Covaris, Woburn, MA, USA) andSciclone-G3 Liquid Handling Workstation (Revvity, USA)), and short NGS sequencing (NovaSeq 6000 and NextSeq 2000 (Illumina, San Diego, CA, USA)). .. Long NGS sequencing platforms (Oxford Nanopore, Oxford, UK) are also currently being imported for the project. .. The DNA sequence pipeline of the DRAGEN Bio-IT Platform (version 3.9.5, Illumina) is used to perform the primary bioinformatics analysis of the NGS sequencing data.A sample biobank and a data storage facility were established to serve sample and data storage, respectively.

    Article Title: Feasibility of long-read sequencing to identify molecular alterations in an Indonesian cohort of locally advanced to advanced nasopharyngeal cancer
    Article Snippet: .. Primarily due to the availability long read sequencers, for example NGS from Oxford Nanopore or PacBio, which can better capture major SVs . ..

    Article Title: Desiccation-induced DNA damage facilitates drug resistance in Mycobacterium tuberculosis
    Article Snippet: Plasmid DNA from colony-derived culture was isolated for transformation into Mtb using a Qiagen miniprep kit Electroporation into competent H37Rv and plating on kanamycin selective 7H10 agar yielded Mtb strains capable of ATC-dependent targeted gene silencing. .. Appropriate sequence positioning and alignment was confirmed by oxford nanopore NGS. .. Cell-laden filters were plunged into 1.7 mL threaded tubes with O-ring seal containing cold 1 mL Trizol (Aldrich chemical) and 200 μL of 0.1 mm Zirconia silica beads (Fisher Scientific).

    Article Title: Large scale monitoring of terrestrial small mammals using noninvasive sampling and COI barcoding
    Article Snippet: .. In fact, NGS sequencing such as Oxford Nanopore (ONT) or Illumina technologies are suited for COI amplicon sequencing. ..

    other:

    Article Title: Report on influenza viruses received and tested by the Melbourne WHO Collaborating Centre for Reference and Research on Influenza during 2023.
    Article Snippet: Whole genome sequencing (WGS) of a smaller subset of viruses was performed by next generation sequencing (NGS) using Illumina iSeq100 or Oxford Nanopore Technology (ONT) MinION platforms according to the manufacturer’s recommendations.

    Article Title: Should we consider tularemia in travelers returning from North Africa?
    Article Snippet: Finally, fine needle aspiration of the suppurated lymph node was performed and PCR amplification and sequencing of the gene coding for the 16S rRNA (Next Generation Sequence, Oxford Nanopore Technologies) revealed F. tularensis confirming the diagnosis.

    Sequencing:

    Article Title: Egypt Genome: Towards an African new genomic era
    Article Snippet: Several copies of various automated equipment were purchased and installed in the lab, including instruments for DNA extraction (chemagicTM 360, Perkin Elmer, Waltham, MA, USA), DNA quality assurance (Qubit 4 Fluorometer (Invitrogen, Carlsbad, CA, USA), NanoDropTM One (Thermo Scientific, Waltham, MA, USA) andLabChip GX Touch (Perkin Elmer)), NGS library preparation (ultrasonicator M220 (Covaris, Woburn, MA, USA) andSciclone-G3 Liquid Handling Workstation (Revvity, USA)), and short NGS sequencing (NovaSeq 6000 and NextSeq 2000 (Illumina, San Diego, CA, USA)). .. Long NGS sequencing platforms (Oxford Nanopore, Oxford, UK) are also currently being imported for the project. .. The DNA sequence pipeline of the DRAGEN Bio-IT Platform (version 3.9.5, Illumina) is used to perform the primary bioinformatics analysis of the NGS sequencing data.A sample biobank and a data storage facility were established to serve sample and data storage, respectively.

    Article Title: Desiccation-induced DNA damage facilitates drug resistance in Mycobacterium tuberculosis
    Article Snippet: Plasmid DNA from colony-derived culture was isolated for transformation into Mtb using a Qiagen miniprep kit Electroporation into competent H37Rv and plating on kanamycin selective 7H10 agar yielded Mtb strains capable of ATC-dependent targeted gene silencing. .. Appropriate sequence positioning and alignment was confirmed by oxford nanopore NGS. .. Cell-laden filters were plunged into 1.7 mL threaded tubes with O-ring seal containing cold 1 mL Trizol (Aldrich chemical) and 200 μL of 0.1 mm Zirconia silica beads (Fisher Scientific).

    Article Title: Large scale monitoring of terrestrial small mammals using noninvasive sampling and COI barcoding
    Article Snippet: .. In fact, NGS sequencing such as Oxford Nanopore (ONT) or Illumina technologies are suited for COI amplicon sequencing. ..

    Amplification:

    Article Title: Large scale monitoring of terrestrial small mammals using noninvasive sampling and COI barcoding
    Article Snippet: .. In fact, NGS sequencing such as Oxford Nanopore (ONT) or Illumina technologies are suited for COI amplicon sequencing. ..



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    Image Search Results


    Sequence of measured nucleotides in a fragment of mtDNA of NIST RM 8256 (A \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = adenine, C \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = cytosine, G \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = guanine, T \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = thymine), and ASCII codes representing the corresponding Phred quality scores

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Robust discrimination between closely related species of salmon based on DNA fragments

    doi: 10.1007/s00216-024-05724-9

    Figure Lengend Snippet: Sequence of measured nucleotides in a fragment of mtDNA of NIST RM 8256 (A \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = adenine, C \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = cytosine, G \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = guanine, T \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = thymine), and ASCII codes representing the corresponding Phred quality scores

    Article Snippet: We have developed a novel method to quantify the confidence surrounding the ability of a representative NextGen Sequencing method to verify the genetic identity of RM 8256, Wild-caught Coho Salmon [ ], which is a fresh frozen fish homogenate prepared from multiple individuals collected off the coast of Alaska, USA.

    Techniques: Sequencing

    The first line has the same sequence listed in Fig. as it was measured, and the second line has one of the 25 replicates that were generated for it based on the site-specific identification error probabilities. This particular replicate differs from the measured sequence in the seven sites marked in red

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Robust discrimination between closely related species of salmon based on DNA fragments

    doi: 10.1007/s00216-024-05724-9

    Figure Lengend Snippet: The first line has the same sequence listed in Fig. as it was measured, and the second line has one of the 25 replicates that were generated for it based on the site-specific identification error probabilities. This particular replicate differs from the measured sequence in the seven sites marked in red

    Article Snippet: We have developed a novel method to quantify the confidence surrounding the ability of a representative NextGen Sequencing method to verify the genetic identity of RM 8256, Wild-caught Coho Salmon [ ], which is a fresh frozen fish homogenate prepared from multiple individuals collected off the coast of Alaska, USA.

    Techniques: Sequencing, Generated

    The top section labeled “MC Replicate” has the “perturbed” sequence listed in the second line of Fig. , from one of the 16 versions of the ensemble of the high-accuracy alignments that Muscle 5.2 produces in this case. The bottom section labeled “Coho Reference Sequence” has the 654-nucleotides long reference sequence for Coho salmon. The “X” indicate “missing” sites in the replicate. The DL distance between these two sequences, disregarding all these “missing” sites, is 10

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Robust discrimination between closely related species of salmon based on DNA fragments

    doi: 10.1007/s00216-024-05724-9

    Figure Lengend Snippet: The top section labeled “MC Replicate” has the “perturbed” sequence listed in the second line of Fig. , from one of the 16 versions of the ensemble of the high-accuracy alignments that Muscle 5.2 produces in this case. The bottom section labeled “Coho Reference Sequence” has the 654-nucleotides long reference sequence for Coho salmon. The “X” indicate “missing” sites in the replicate. The DL distance between these two sequences, disregarding all these “missing” sites, is 10

    Article Snippet: We have developed a novel method to quantify the confidence surrounding the ability of a representative NextGen Sequencing method to verify the genetic identity of RM 8256, Wild-caught Coho Salmon [ ], which is a fresh frozen fish homogenate prepared from multiple individuals collected off the coast of Alaska, USA.

    Techniques: Labeling, Sequencing