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next-generation sequencing libraries  (Nextera AS)

 
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    Nextera AS next-generation sequencing libraries
    Next Generation Sequencing Libraries, supplied by Nextera AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequencing+libraries/next+generation+sequencing+libraries/pm30059786-357-2-6
    Average 90 stars, based on 1 article reviews
    next-generation sequencing libraries - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: Cas12a/Cpf1 knock-in mice enable efficient multiplexed immune cell engineering
    Article Snippet: .. Nextera library preparation followed by Next Generation Sequencing was used to quantify the gene editing efficiency on molecular level. .. For all crRNAs targeting surface proteins in CD4 and CD8 T cells, higher indel percentages were observed compared to vector controls ( ). crCD4-1 generated the highest (66%) indels for the CD4 T cells ( , ).

    Article Title: Characterization of chromatin accessibility with a transposome hypersensitive sites sequencing (THS-seq) assay.
    Article Snippet: .. Caruccio N. Preparation of next-generation sequencing libraries using Nextera technology: simultaneous DNA fragmentation and adaptor tagging by in vitro transposition. ..

    Article Title: Striking antibody evasion manifested by the Omicron variant of SARS-CoV-2.
    Article Snippet: .. To verify the sequences of variants, next-generation sequencing libraries were prepared according to a low-volume Nextera sequencing protocol43 and sequenced on the Illumina Miseq platform (single-end mode with 50 bp R1). ..

    Article Title: In vivo CRISPR screening in CD8 T cells with AAV-Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma.
    Article Snippet: .. DNA level analyses by surveyor assay and Nextera next generation sequencing demonstrated that PDIA3 knockout was highly efficient (86%) (Fig. 5b–d). ..

    Article Title: Multi-omic elucidation of aromatic catabolism in adaptively evolved Rhodococcus opacus.
    Article Snippet: .. Preparation of next-generation sequencing libraries using Nextera technology: simultaneous DNA fragmentation and adaptor tagging by in vitro transposition. ..

    In Vitro:

    Article Title: Characterization of chromatin accessibility with a transposome hypersensitive sites sequencing (THS-seq) assay.
    Article Snippet: .. Caruccio N. Preparation of next-generation sequencing libraries using Nextera technology: simultaneous DNA fragmentation and adaptor tagging by in vitro transposition. ..

    Article Title: Multi-omic elucidation of aromatic catabolism in adaptively evolved Rhodococcus opacus.
    Article Snippet: .. Preparation of next-generation sequencing libraries using Nextera technology: simultaneous DNA fragmentation and adaptor tagging by in vitro transposition. ..

    other:

    Article Title: Antifungal Triazole Posaconazole Targets an Early Stage of the Parechovirus A3 Life Cycle
    Article Snippet: Viruses from Vero-P passages 0 through 14 were sequenced using a Nextera XT next-generation sequencing (NGS) library kit and a 300-cycle (2 150-bp paired-end) MiSeq reagent kit, v2 (Illumina, San Diego, CA), as described previously (46).

    Article Title: Antifungal Triazole Posaconazole Targets an Early Stage of the Parechovirus A3 Life Cycle
    Article Snippet: Viruses from Vero-P passages 0 through 14 were sequenced using a Nextera XT next-generation sequencing (NGS) library kit and a 300-cycle (2× 150-bp paired-end) MiSeq reagent kit, v2 (Illumina, San Diego, CA), as described previously ( 46 ).

    Article Title: Whole genome sequencing-based drug resistance predictions of multidrug-resistant Mycobacterium tuberculosis isolates from Tanzania
    Article Snippet: Libraries for next generation sequencing (NGS) were prepared from gDNA using a modified Nextera protocol.

    Sequencing:

    Article Title: Striking antibody evasion manifested by the Omicron variant of SARS-CoV-2.
    Article Snippet: .. To verify the sequences of variants, next-generation sequencing libraries were prepared according to a low-volume Nextera sequencing protocol43 and sequenced on the Illumina Miseq platform (single-end mode with 50 bp R1). ..

    Knock-Out:

    Article Title: In vivo CRISPR screening in CD8 T cells with AAV-Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma.
    Article Snippet: .. DNA level analyses by surveyor assay and Nextera next generation sequencing demonstrated that PDIA3 knockout was highly efficient (86%) (Fig. 5b–d). ..



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    Image Search Results


    Normalized CDKN2A transcript counts from RNA sequencing according to HPV/p16 status in our cohort

    Journal: bioRxiv

    Article Title: Dysregulation of the p16–CDK6 axis characterizes HPV-unrelated p16-positive oropharyngeal carcinoma

    doi: 10.64898/2026.08.17.743010

    Figure Lengend Snippet: Normalized CDKN2A transcript counts from RNA sequencing according to HPV/p16 status in our cohort

    Article Snippet: Sequencing libraries were generated with the Twist EF 2.0 Library Preparation Kit (Twist Biosciences, South San Francisco, CA, USA).

    Techniques: RNA Sequencing

    (A) STRING network visualization of genes in the “Pathways in cancer” pathway that were commonly upregulated in HPV-unrelated OPC. (B) Schematic diagram of the p16–RB pathway. (C) CDK6 expression in RNA sequencing data stratified by HPV and CDKN2A status in the TCGA dataset. (D) Representative immunohistochemistry images of CDK6 staining. Scale bar, 200 μm. (E) IHC scores for CDK6 staining. (F) Western blot showing p16 expression in RPE1 cells with doxycycline-inducible CDK6 expression. (G) Western blot showing CDK6 expression following p16 transduction in CDKN2A-deficient FaDu cells. (H) Proportion of CDK6 expression levels in leukoplakia. CDK6 immunohistochemical staining was evaluated using a scoring system, and the distribution of scores is shown by histopathological grade. ND/LGD, non-dysplastic/low-grade dysplasia; HGD, high-grade dysplasia; SCC, squamous cell carcinoma in situ or carcinoma with minimal invasion. (I) HE and immunohistochemical images of oral leukoplakia with high-grade dysplasia showing p16 expression. Scale bars, 50 μm. (J) Clonal expansion of CDKN2A-deficient cells in CDK6-overexpressing RPE1 cells. The fraction of CDKN2A indels was quantified over time in RPE1 cells expressing the CDK6 (R31C) mutant in a doxycycline-dependent manner. DOX, doxycycline. Statistical analyses were performed using the Mann–Whitney U test in (C) and (E), and Welch’s t-test in (J). **p < 0.01, ***p < 0.001. n.s., not significant.

    Journal: bioRxiv

    Article Title: Dysregulation of the p16–CDK6 axis characterizes HPV-unrelated p16-positive oropharyngeal carcinoma

    doi: 10.64898/2026.08.17.743010

    Figure Lengend Snippet: (A) STRING network visualization of genes in the “Pathways in cancer” pathway that were commonly upregulated in HPV-unrelated OPC. (B) Schematic diagram of the p16–RB pathway. (C) CDK6 expression in RNA sequencing data stratified by HPV and CDKN2A status in the TCGA dataset. (D) Representative immunohistochemistry images of CDK6 staining. Scale bar, 200 μm. (E) IHC scores for CDK6 staining. (F) Western blot showing p16 expression in RPE1 cells with doxycycline-inducible CDK6 expression. (G) Western blot showing CDK6 expression following p16 transduction in CDKN2A-deficient FaDu cells. (H) Proportion of CDK6 expression levels in leukoplakia. CDK6 immunohistochemical staining was evaluated using a scoring system, and the distribution of scores is shown by histopathological grade. ND/LGD, non-dysplastic/low-grade dysplasia; HGD, high-grade dysplasia; SCC, squamous cell carcinoma in situ or carcinoma with minimal invasion. (I) HE and immunohistochemical images of oral leukoplakia with high-grade dysplasia showing p16 expression. Scale bars, 50 μm. (J) Clonal expansion of CDKN2A-deficient cells in CDK6-overexpressing RPE1 cells. The fraction of CDKN2A indels was quantified over time in RPE1 cells expressing the CDK6 (R31C) mutant in a doxycycline-dependent manner. DOX, doxycycline. Statistical analyses were performed using the Mann–Whitney U test in (C) and (E), and Welch’s t-test in (J). **p < 0.01, ***p < 0.001. n.s., not significant.

    Article Snippet: Sequencing libraries were generated with the Twist EF 2.0 Library Preparation Kit (Twist Biosciences, South San Francisco, CA, USA).

    Techniques: Expressing, RNA Sequencing, Immunohistochemistry, Staining, Western Blot, Transduction, Immunohistochemical staining, In Situ, Mutagenesis, MANN-WHITNEY

    (A) Schematic illustration of the CDKN2A alteration inferred from oncopanel sequencing in Case IV shown in . (B) IGV view of RNA sequencing read alignments flanking the CDKN2A deletion in the same case.

    Journal: bioRxiv

    Article Title: Dysregulation of the p16–CDK6 axis characterizes HPV-unrelated p16-positive oropharyngeal carcinoma

    doi: 10.64898/2026.08.17.743010

    Figure Lengend Snippet: (A) Schematic illustration of the CDKN2A alteration inferred from oncopanel sequencing in Case IV shown in . (B) IGV view of RNA sequencing read alignments flanking the CDKN2A deletion in the same case.

    Article Snippet: Sequencing libraries were generated with the Twist EF 2.0 Library Preparation Kit (Twist Biosciences, South San Francisco, CA, USA).

    Techniques: Sequencing, RNA Sequencing