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genotyping 21  (Plasmidsaurus)


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    Structured Review

    Plasmidsaurus genotyping 21
    Genotyping 21, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequence+analysis/Genotyping+Analysis/pm41004705-29-2-24
    Average 94 stars, based on 1 article reviews
    genotyping 21 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Acyl-coA binding protein AcbdA is required for peroxisome hitchhiking on early endosomes in <i>Aspergillus nidulans</i>
    Article Snippet: Motor-driven transport on microtubules is critical for distributing organelles throughout the cell.. Most commonly, organelle movement is mediated by cargo adaptors, proteins on the surface of an organelle that directly recruit microtubule-based motors.. An alternative mechanism called hitchhiking was recently discovered: some organelles move, not by recruiting the motors directly, but instead by using membrane contact sites to attach to motor-driven vesicles and hitchhike along microtubules.

    Article Title: Distinct requirements for the C. elegans Delta ligand APX-1 in embryonic viability and adult fertility.
    Article Snippet: The apx-1(tm3438) deletion allele was provide by the 17 National Bioresource Project in Japan; our generation of the apx-1(or2015) is described 18 below. .. 19 20 Genotyping 21 All 8 apx-1 exons and partial introns of or3, or15, or22 and or545ts were commercially 22 sequenced using long-read sequencing (Plasmidsaurus) after PCR amplification and 23 compared to the apx-1 sequence listed in WormBase (version WS297). ..

    Article Title: Acyl-coA binding protein AcbdA regulates peroxisome hitchhiking on early endosomes
    Article Snippet: To amplify the linearized DNA used for transforming protoplasts, the oligos 5’-CAATGCTTTCACGGGCGTCACCACG-3’ and 5’ CCGCTCCGTCCGGTAACTGAGGTCA-3’ were used. .. This strain was confirmed by genotyping PCR followed by Plasmidsaurus nanopore sequencing as described above. ..

    Article Title: Application of AI-Designed OpenCRISPR-1 for Highly Efficient Gene Editing in Soybean and Nicotiana benthamiana
    Article Snippet: .. For genotyping by amplicon sequencing, PCR products were purified using a DNA extraction kit (K0691, Thermo Fisher Scientific) and subsequently submitted to Plasmidsaurus for Genotyping Analysis (Oxford Nanopore, R10.4.1). .. For Sanger Sequencing, purified PCR products were ligated into the pJET1.2 cloning system (K1232, Thermo Fisher Scientific) before being sent for sequencing.

    Nanopore Sequencing:

    Article Title: Acyl-coA binding protein AcbdA is required for peroxisome hitchhiking on early endosomes in <i>Aspergillus nidulans</i>
    Article Snippet: Motor-driven transport on microtubules is critical for distributing organelles throughout the cell.. Most commonly, organelle movement is mediated by cargo adaptors, proteins on the surface of an organelle that directly recruit microtubule-based motors.. An alternative mechanism called hitchhiking was recently discovered: some organelles move, not by recruiting the motors directly, but instead by using membrane contact sites to attach to motor-driven vesicles and hitchhike along microtubules.

    Article Title: Acyl-coA binding protein AcbdA regulates peroxisome hitchhiking on early endosomes
    Article Snippet: To amplify the linearized DNA used for transforming protoplasts, the oligos 5’-CAATGCTTTCACGGGCGTCACCACG-3’ and 5’ CCGCTCCGTCCGGTAACTGAGGTCA-3’ were used. .. This strain was confirmed by genotyping PCR followed by Plasmidsaurus nanopore sequencing as described above. ..

    Functional Assay:

    Article Title: Acyl-coA binding protein AcbdA is required for peroxisome hitchhiking on early endosomes in <i>Aspergillus nidulans</i>
    Article Snippet: Motor-driven transport on microtubules is critical for distributing organelles throughout the cell.. Most commonly, organelle movement is mediated by cargo adaptors, proteins on the surface of an organelle that directly recruit microtubule-based motors.. An alternative mechanism called hitchhiking was recently discovered: some organelles move, not by recruiting the motors directly, but instead by using membrane contact sites to attach to motor-driven vesicles and hitchhike along microtubules.

    Plasmid Preparation:

    Article Title: Acyl-coA binding protein AcbdA is required for peroxisome hitchhiking on early endosomes in <i>Aspergillus nidulans</i>
    Article Snippet: Motor-driven transport on microtubules is critical for distributing organelles throughout the cell.. Most commonly, organelle movement is mediated by cargo adaptors, proteins on the surface of an organelle that directly recruit microtubule-based motors.. An alternative mechanism called hitchhiking was recently discovered: some organelles move, not by recruiting the motors directly, but instead by using membrane contact sites to attach to motor-driven vesicles and hitchhike along microtubules.

    Construct:

    Article Title: Acyl-coA binding protein AcbdA is required for peroxisome hitchhiking on early endosomes in <i>Aspergillus nidulans</i>
    Article Snippet: Motor-driven transport on microtubules is critical for distributing organelles throughout the cell.. Most commonly, organelle movement is mediated by cargo adaptors, proteins on the surface of an organelle that directly recruit microtubule-based motors.. An alternative mechanism called hitchhiking was recently discovered: some organelles move, not by recruiting the motors directly, but instead by using membrane contact sites to attach to motor-driven vesicles and hitchhike along microtubules.

    Sequencing:

    Article Title: Distinct requirements for the C. elegans Delta ligand APX-1 in embryonic viability and adult fertility.
    Article Snippet: The apx-1(tm3438) deletion allele was provide by the 17 National Bioresource Project in Japan; our generation of the apx-1(or2015) is described 18 below. .. 19 20 Genotyping 21 All 8 apx-1 exons and partial introns of or3, or15, or22 and or545ts were commercially 22 sequenced using long-read sequencing (Plasmidsaurus) after PCR amplification and 23 compared to the apx-1 sequence listed in WormBase (version WS297). ..

    Article Title: Application of AI-Designed OpenCRISPR-1 for Highly Efficient Gene Editing in Soybean and Nicotiana benthamiana
    Article Snippet: .. For genotyping by amplicon sequencing, PCR products were purified using a DNA extraction kit (K0691, Thermo Fisher Scientific) and subsequently submitted to Plasmidsaurus for Genotyping Analysis (Oxford Nanopore, R10.4.1). .. For Sanger Sequencing, purified PCR products were ligated into the pJET1.2 cloning system (K1232, Thermo Fisher Scientific) before being sent for sequencing.

    Amplification:

    Article Title: Distinct requirements for the C. elegans Delta ligand APX-1 in embryonic viability and adult fertility.
    Article Snippet: The apx-1(tm3438) deletion allele was provide by the 17 National Bioresource Project in Japan; our generation of the apx-1(or2015) is described 18 below. .. 19 20 Genotyping 21 All 8 apx-1 exons and partial introns of or3, or15, or22 and or545ts were commercially 22 sequenced using long-read sequencing (Plasmidsaurus) after PCR amplification and 23 compared to the apx-1 sequence listed in WormBase (version WS297). ..

    Article Title: Application of AI-Designed OpenCRISPR-1 for Highly Efficient Gene Editing in Soybean and Nicotiana benthamiana
    Article Snippet: .. For genotyping by amplicon sequencing, PCR products were purified using a DNA extraction kit (K0691, Thermo Fisher Scientific) and subsequently submitted to Plasmidsaurus for Genotyping Analysis (Oxford Nanopore, R10.4.1). .. For Sanger Sequencing, purified PCR products were ligated into the pJET1.2 cloning system (K1232, Thermo Fisher Scientific) before being sent for sequencing.

    Purification:

    Article Title: Application of AI-Designed OpenCRISPR-1 for Highly Efficient Gene Editing in Soybean and Nicotiana benthamiana
    Article Snippet: .. For genotyping by amplicon sequencing, PCR products were purified using a DNA extraction kit (K0691, Thermo Fisher Scientific) and subsequently submitted to Plasmidsaurus for Genotyping Analysis (Oxford Nanopore, R10.4.1). .. For Sanger Sequencing, purified PCR products were ligated into the pJET1.2 cloning system (K1232, Thermo Fisher Scientific) before being sent for sequencing.

    DNA Extraction:

    Article Title: Application of AI-Designed OpenCRISPR-1 for Highly Efficient Gene Editing in Soybean and Nicotiana benthamiana
    Article Snippet: .. For genotyping by amplicon sequencing, PCR products were purified using a DNA extraction kit (K0691, Thermo Fisher Scientific) and subsequently submitted to Plasmidsaurus for Genotyping Analysis (Oxford Nanopore, R10.4.1). .. For Sanger Sequencing, purified PCR products were ligated into the pJET1.2 cloning system (K1232, Thermo Fisher Scientific) before being sent for sequencing.



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