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OmicSoft Corporation sequence aligner
Sequence Aligner, supplied by OmicSoft Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequence+alignment/sequence+aligner/pmc06383382-74-24-24
Average 90 stars, based on 1 article reviews
sequence aligner - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Gene Expression:

Article Title: Association between gene expression signatures and clinical outcomes of pembrolizumab versus paclitaxel in advanced gastric cancer: exploratory analysis from the randomized, controlled, phase III KEYNOTE-061 trial.
Article Snippet: RNA sequencing was performed on formalin- fixed, paraffin- embedded (FFPE) tumor tissue samples provided at screening using the HiSeq 4000 platform (Illumina, California, USA). .. Of note, the gene expression signatures performed similarly in gene expression data obtained from freshly frozen and FFPE samples.12 The RNA- sequencing raw reads were processed using a customized RNA- sequencing data analysis pipeline in OmicSoft ArraySuite version 9 (Qiagen, Hilden, Germany) as previously described.12 First, the raw reads were quality- filtered and then aligned to the reference genome Human.B37.3 using the Omicsoft sequence aligner.19 Thereafter, gene expression levels, defined as raw read counts and fragments per kilobase of exon per million mapped fragments, were quantified using the RNA sequencing by expectation maximization algorithm with the Ensembl.R75 gene model.20 TcellinfGEP score was calculated as the weighted sum of normalized expression values for the 18 genes determined as predictors of response in the pantumor setting on the NanoString platform.12 13 The 10 non- TcellinfGEP signature scores were calculated as the average of the genes (on the logarithmic scale) in each signature gene set as previously described.12 13 TMB was determined via whole exome sequencing (mut/exome) of tumor samples and matched DNA. ..

Article Title: Baseline and post-treatment biomarkers of resistance to anti-PD-1 therapy in acral and mucosal melanoma: an observational study.
Article Snippet: All sequencing was performed on the NovaSeq 6000 (Illumina) with 2×51 bp read length. .. FASTQ files were generated and sequencing reads aligned to human GRCh37/hg19 using the Omicsoft sequence aligner.52 Gene expression levels were quantified by upper quantile normalized fragments per kilobyte of transcript per million mapped reads (log10). ..

Formalin-fixed Paraffin-Embedded:

Article Title: Association between gene expression signatures and clinical outcomes of pembrolizumab versus paclitaxel in advanced gastric cancer: exploratory analysis from the randomized, controlled, phase III KEYNOTE-061 trial.
Article Snippet: RNA sequencing was performed on formalin- fixed, paraffin- embedded (FFPE) tumor tissue samples provided at screening using the HiSeq 4000 platform (Illumina, California, USA). .. Of note, the gene expression signatures performed similarly in gene expression data obtained from freshly frozen and FFPE samples.12 The RNA- sequencing raw reads were processed using a customized RNA- sequencing data analysis pipeline in OmicSoft ArraySuite version 9 (Qiagen, Hilden, Germany) as previously described.12 First, the raw reads were quality- filtered and then aligned to the reference genome Human.B37.3 using the Omicsoft sequence aligner.19 Thereafter, gene expression levels, defined as raw read counts and fragments per kilobase of exon per million mapped fragments, were quantified using the RNA sequencing by expectation maximization algorithm with the Ensembl.R75 gene model.20 TcellinfGEP score was calculated as the weighted sum of normalized expression values for the 18 genes determined as predictors of response in the pantumor setting on the NanoString platform.12 13 The 10 non- TcellinfGEP signature scores were calculated as the average of the genes (on the logarithmic scale) in each signature gene set as previously described.12 13 TMB was determined via whole exome sequencing (mut/exome) of tumor samples and matched DNA. ..

RNA Sequencing:

Article Title: Association between gene expression signatures and clinical outcomes of pembrolizumab versus paclitaxel in advanced gastric cancer: exploratory analysis from the randomized, controlled, phase III KEYNOTE-061 trial.
Article Snippet: RNA sequencing was performed on formalin- fixed, paraffin- embedded (FFPE) tumor tissue samples provided at screening using the HiSeq 4000 platform (Illumina, California, USA). .. Of note, the gene expression signatures performed similarly in gene expression data obtained from freshly frozen and FFPE samples.12 The RNA- sequencing raw reads were processed using a customized RNA- sequencing data analysis pipeline in OmicSoft ArraySuite version 9 (Qiagen, Hilden, Germany) as previously described.12 First, the raw reads were quality- filtered and then aligned to the reference genome Human.B37.3 using the Omicsoft sequence aligner.19 Thereafter, gene expression levels, defined as raw read counts and fragments per kilobase of exon per million mapped fragments, were quantified using the RNA sequencing by expectation maximization algorithm with the Ensembl.R75 gene model.20 TcellinfGEP score was calculated as the weighted sum of normalized expression values for the 18 genes determined as predictors of response in the pantumor setting on the NanoString platform.12 13 The 10 non- TcellinfGEP signature scores were calculated as the average of the genes (on the logarithmic scale) in each signature gene set as previously described.12 13 TMB was determined via whole exome sequencing (mut/exome) of tumor samples and matched DNA. ..

Sequencing:

Article Title: Association between gene expression signatures and clinical outcomes of pembrolizumab versus paclitaxel in advanced gastric cancer: exploratory analysis from the randomized, controlled, phase III KEYNOTE-061 trial.
Article Snippet: RNA sequencing was performed on formalin- fixed, paraffin- embedded (FFPE) tumor tissue samples provided at screening using the HiSeq 4000 platform (Illumina, California, USA). .. Of note, the gene expression signatures performed similarly in gene expression data obtained from freshly frozen and FFPE samples.12 The RNA- sequencing raw reads were processed using a customized RNA- sequencing data analysis pipeline in OmicSoft ArraySuite version 9 (Qiagen, Hilden, Germany) as previously described.12 First, the raw reads were quality- filtered and then aligned to the reference genome Human.B37.3 using the Omicsoft sequence aligner.19 Thereafter, gene expression levels, defined as raw read counts and fragments per kilobase of exon per million mapped fragments, were quantified using the RNA sequencing by expectation maximization algorithm with the Ensembl.R75 gene model.20 TcellinfGEP score was calculated as the weighted sum of normalized expression values for the 18 genes determined as predictors of response in the pantumor setting on the NanoString platform.12 13 The 10 non- TcellinfGEP signature scores were calculated as the average of the genes (on the logarithmic scale) in each signature gene set as previously described.12 13 TMB was determined via whole exome sequencing (mut/exome) of tumor samples and matched DNA. ..

Article Title: Association between gene expression signatures and clinical outcomes of pembrolizumab versus paclitaxel in advanced gastric cancer: exploratory analysis from the randomized, controlled, phase III KEYNOTE-061 trial
Article Snippet: The RNA-sequencing raw reads were processed using a customized RNA-sequencing data analysis pipeline in OmicSoft ArraySuite version 9 (Qiagen, Hilden, Germany) as previously described. .. First, the raw reads were quality-filtered and then aligned to the reference genome Human.B37.3 using the Omicsoft sequence aligner. .. Thereafter, gene expression levels, defined as raw read counts and fragments per kilobase of exon per million mapped fragments, were quantified using the RNA sequencing by expectation maximization algorithm with the Ensembl.R75 gene model. Tcell inf GEP score was calculated as the weighted sum of normalized expression values for the 18 genes determined as predictors of response in the pan-tumor setting on the NanoString platform.

Article Title: First-in-Class Anti-immunoglobulin–like Transcript 4 Myeloid-Specific Antibody MK-4830 Abrogates a PD-1 Resistance Mechanism in Patients with Advanced Solid Tumors
Article Snippet: Gene-level fragments per kilobase million (FPKM) values were generated in Omicsoft Array Studio v11.0 (Qiagen) according to the established RNA sequencing pipeline. .. Omicsoft sequence aligner as alignment algorithm, Human.B37.3 as alignment reference, and Ensembl.R75 as gene model were used. ..

Article Title: Baseline and post-treatment biomarkers of resistance to anti-PD-1 therapy in acral and mucosal melanoma: an observational study.
Article Snippet: All sequencing was performed on the NovaSeq 6000 (Illumina) with 2×51 bp read length. .. FASTQ files were generated and sequencing reads aligned to human GRCh37/hg19 using the Omicsoft sequence aligner.52 Gene expression levels were quantified by upper quantile normalized fragments per kilobyte of transcript per million mapped reads (log10). ..

Article Title: Baseline and post-treatment biomarkers of resistance to anti-PD-1 therapy in acral and mucosal melanoma: an observational study
Article Snippet: All sequencing was performed on the NovaSeq 6000 (Illumina) with 2×51 bp read length. .. FASTQ files were generated and sequencing reads aligned to human GRCh37/hg19 using the Omicsoft sequence aligner. ..

Expressing:

Article Title: Association between gene expression signatures and clinical outcomes of pembrolizumab versus paclitaxel in advanced gastric cancer: exploratory analysis from the randomized, controlled, phase III KEYNOTE-061 trial.
Article Snippet: RNA sequencing was performed on formalin- fixed, paraffin- embedded (FFPE) tumor tissue samples provided at screening using the HiSeq 4000 platform (Illumina, California, USA). .. Of note, the gene expression signatures performed similarly in gene expression data obtained from freshly frozen and FFPE samples.12 The RNA- sequencing raw reads were processed using a customized RNA- sequencing data analysis pipeline in OmicSoft ArraySuite version 9 (Qiagen, Hilden, Germany) as previously described.12 First, the raw reads were quality- filtered and then aligned to the reference genome Human.B37.3 using the Omicsoft sequence aligner.19 Thereafter, gene expression levels, defined as raw read counts and fragments per kilobase of exon per million mapped fragments, were quantified using the RNA sequencing by expectation maximization algorithm with the Ensembl.R75 gene model.20 TcellinfGEP score was calculated as the weighted sum of normalized expression values for the 18 genes determined as predictors of response in the pantumor setting on the NanoString platform.12 13 The 10 non- TcellinfGEP signature scores were calculated as the average of the genes (on the logarithmic scale) in each signature gene set as previously described.12 13 TMB was determined via whole exome sequencing (mut/exome) of tumor samples and matched DNA. ..

other:

Article Title: Mouse primary microglia respond differently to LPS and poly(I:C) in vitro
Article Snippet: Reads were mapped to mouse GRCm38 genomes ( https://www.ncbi.nlm.nih.gov/grc/ ) using OmicSoft sequence aligner (OSA) of the OmicSoft Suite software.

Article Title: Mechanisms of Impaired Lung Development and Ciliation in Mannosidase-1-Alpha-2 ( Man1a2 ) Mutants.
Article Snippet: Trimmed reads were then aligned to Ensembl mouse reference genome (version Mouse.B38) using the Omicsoft Sequence Aligner (OSA) (Hu et al., 2012) on Array Studio V10.1, with index built to Ensembl genome for cDNA and ncRNA genome sequences.

Article Title: Mechanisms of Impaired Lung Development and Ciliation in Mannosidase-1-Alpha-2 ( Man1a2 ) Mutants
Article Snippet: Trimmed reads were then aligned to Ensembl mouse reference genome (version Mouse.B38) using the Omicsoft Sequence Aligner (OSA) ( ) on Array Studio V10.1, with index built to Ensembl genome for cDNA and ncRNA genome sequences.

Generated:

Article Title: Baseline and post-treatment biomarkers of resistance to anti-PD-1 therapy in acral and mucosal melanoma: an observational study.
Article Snippet: All sequencing was performed on the NovaSeq 6000 (Illumina) with 2×51 bp read length. .. FASTQ files were generated and sequencing reads aligned to human GRCh37/hg19 using the Omicsoft sequence aligner.52 Gene expression levels were quantified by upper quantile normalized fragments per kilobyte of transcript per million mapped reads (log10). ..

Article Title: Baseline and post-treatment biomarkers of resistance to anti-PD-1 therapy in acral and mucosal melanoma: an observational study
Article Snippet: All sequencing was performed on the NovaSeq 6000 (Illumina) with 2×51 bp read length. .. FASTQ files were generated and sequencing reads aligned to human GRCh37/hg19 using the Omicsoft sequence aligner. ..



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