avrii (New England Biolabs)
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avrii
Avrii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/seq+multiple+grna+expression+vector+backbone/AvrII/pmc05047176-210-9-33
Average 97 stars, based on 778 article reviews
Avrii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/seq+multiple+grna+expression+vector+backbone/AvrII/pmc05047176-210-9-33
Average 97 stars, based on 778 article reviews
avrii - by Bioz Stars,
2026-11
97/100 stars
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Expressing:Article Title: Neuronally sensed oxygen drives behavior and development in human-infective, skin-penetrating nematodes Article Snippet: To drive expression in Sst- URX, we used SacI-HF (New England Biolabs, R3156) and AgeI-HF (New England Biolabs, R3552) to clone the Sst-gcy-36 promoter from pBMW15 into pRP08, resulting in pBMW31 ( Sst-gcy-36p::strGCaMP7f:: P2A ::strmScarlet-I::Sst-era-1 3′ UTR). .. To drive expression in Sst- BAG, we used AgeI-HF (New England Biolabs, R3552) and Article Title: Transcriptomic and functional analyses uncover a conserved effector driving genotype-dependent virulence in the Sphaerulina musiva-Populus trichocarpa interaction. Article Snippet: .. Subsequently, the plasmid was linearized using Polymerase Chain Reaction:Article Title: PRIME: A modular plasmid-based platform for continuous in vivo evolution across bacteria Article Snippet: .. The PCR product was digested with Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A Transformation Assay:Article Title: PRIME: A modular plasmid-based platform for continuous in vivo evolution across bacteria Article Snippet: .. The PCR product was digested with Article Title: Transcriptomic and functional analyses uncover a conserved effector driving genotype-dependent virulence in the Sphaerulina musiva-Populus trichocarpa interaction. Article Snippet: .. Subsequently, the plasmid was linearized using Over Expression:Article Title: Loss of flavonol 3‐ O ‐glucosyltransferase activity confers soybean resistance to leaf‐chewing insects Article Snippet: .. Two overexpression vectors used in the complementation assays were built as follows: The coding sequences for Glyma07g14530‐S (the wild‐type, susceptible allele) and Glyma07g14530 ‐R (the resistant, mutant allele) were amplified from leaf DNA isolated from Benning and Benning M respectively, with the primers Asc I‐530‐OE‐F and Mutagenesis:Article Title: Loss of flavonol 3‐ O ‐glucosyltransferase activity confers soybean resistance to leaf‐chewing insects Article Snippet: .. Two overexpression vectors used in the complementation assays were built as follows: The coding sequences for Glyma07g14530‐S (the wild‐type, susceptible allele) and Glyma07g14530 ‐R (the resistant, mutant allele) were amplified from leaf DNA isolated from Benning and Benning M respectively, with the primers Asc I‐530‐OE‐F and Article Title: Progressive cognitive impairment and ventricular tachycardia in a boy with biallelic POLG variants and a de novo RYR2 variation. Article Snippet: .. The MIP1 fragment carrying the variant of interest was digested with NotI-HF and Amplification:Article Title: Loss of flavonol 3‐ O ‐glucosyltransferase activity confers soybean resistance to leaf‐chewing insects Article Snippet: .. Two overexpression vectors used in the complementation assays were built as follows: The coding sequences for Glyma07g14530‐S (the wild‐type, susceptible allele) and Glyma07g14530 ‐R (the resistant, mutant allele) were amplified from leaf DNA isolated from Benning and Benning M respectively, with the primers Asc I‐530‐OE‐F and Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A Isolation:Article Title: Loss of flavonol 3‐ O ‐glucosyltransferase activity confers soybean resistance to leaf‐chewing insects Article Snippet: .. Two overexpression vectors used in the complementation assays were built as follows: The coding sequences for Glyma07g14530‐S (the wild‐type, susceptible allele) and Glyma07g14530 ‐R (the resistant, mutant allele) were amplified from leaf DNA isolated from Benning and Benning M respectively, with the primers Asc I‐530‐OE‐F and Variant Assay:Article Title: Progressive cognitive impairment and ventricular tachycardia in a boy with biallelic POLG variants and a de novo RYR2 variation. Article Snippet: .. The MIP1 fragment carrying the variant of interest was digested with NotI-HF and Sequencing:Article Title: Modified Fc-regions to enhance functional affinity of antibodies and antigen binding fragments thereof Article Snippet: .. Following sequence confirmation and maxiprep, 15 μg of plasmid DNA was digested with AfeI and Plasmid Preparation:Article Title: Modified Fc-regions to enhance functional affinity of antibodies and antigen binding fragments thereof Article Snippet: .. Following sequence confirmation and maxiprep, 15 μg of plasmid DNA was digested with AfeI and Article Title: A versatile fluorescent reporter for Usutu virus drug screening and infection tracking. Article Snippet: .. The following thermocycler method was used: 98 °C for 1 min; 18 cycles of 98 °C for 10 s, 65 °C for 15 s, 72 °C for 50 s; 72 °C for 1 min. To generate the vector backbone, the original CPER fragment 2 plasmid was digested with Article Title: Transcriptomic and functional analyses uncover a conserved effector driving genotype-dependent virulence in the Sphaerulina musiva-Populus trichocarpa interaction. Article Snippet: .. Subsequently, the plasmid was linearized using Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A Purification:Article Title: Modified Fc-regions to enhance functional affinity of antibodies and antigen binding fragments thereof Article Snippet: .. Following sequence confirmation and maxiprep, 15 μg of plasmid DNA was digested with AfeI and Clone Assay:Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A Introduce:Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A |