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semiconductor-based electrochemical-synthesis process  (CustomArray Inc)

 
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    CustomArray Inc semiconductor-based electrochemical-synthesis process
    Semiconductor Based Electrochemical Synthesis Process, supplied by CustomArray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/semiconductor-based+desktop+microarray+platform/semiconductor+based+electrochemical+detritylation+synthesis/us09928869-141-16-1
    Average 90 stars, based on 1 article reviews
    semiconductor-based electrochemical-synthesis process - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: In vivo CRISPR knockout screen identifies p47 as a suppressor of HER2+ breast cancer metastasis by regulating NEMO trafficking and autophagy flux.
    Article Snippet: Generation of a custom CRISPR knockout library targeting autophagy regulatory genes and in vivo CRISPR screens 1,026 single oligonucleotides targeting 171 mice genes and 100 nontargeting controls were synthesized using a semiconductorbased electrochemical detritylation synthesis (CustomArray).

    Article Title: Correction: Recent progress in DNA data storage based on high-throughput DNA synthesis
    Article Snippet: Reproduced with permission from [95]. . Fig. 8 Open in a separate window In contrast to binary data, each base of nucleotides has the distinct signal distributions and spacing. a Nanopore sequencing signal distribution. b Illumina sequencing’s three different fluorescent signal measurement for A, G, T, and C detection. . Fig. 9 Open in a separate window The photochemical deprotection and coupling steps for oligonucleotide synthesis. (1) The 5’-end of oligonucleotide is blocked by a protecting group. (2) Patterned UV light deprotects the protecting group. (3) The deprotected oligonucleotide exposes the hydroxyl group. (4) Desired 5’-protected nucleoside (e.g., G) is introduced into the flow cell, initiating a coupling reaction with the hydroxyl group. (5) Repeat steps (2)–(4) with the other bases of nucleotides (e.g., T, C, and A). . Fig. 10 Open in a separate window Error factors in array-based oligonucleotide synthesis methods. a Photochemical: the edge gradient of light exposure results in stochastic deprotection. b Electrochemical: acid diffusion contributes to crosstalk. c Inkjet printer: misalignment of each droplet leads to deletion error. . Fig. 11 Open in a separate window Patents addressing limitations in array-based oligonucleotide synthesis methods. a To confine the reaction area to the uniform illumination region, a self-aligned micropattern is implemented using a DMD through image reversal to block the edge of the feature in the photochemical method. b CustomArray utilizes electrochemical synthesis on a CMOS chip with a virtual flask to quench diffused protons. c Twist Bioscience employs a silicon-based cluster substrate for DNA assembly using the inkjet printer method.

    Microarray:

    Article Title: Methods for retrievable information storage using nucleic acids
    Article Snippet: .. The CustomArray system uses a pH gradient and synthesizes a desired oligonucleotide microarray using a semiconductor-based electrochemical-synthesis process. ..

    Biomarker Discovery:

    Article Title: CRISPR mediated in vivo modeling and genetic screening of tumor growth and metastasis
    Article Snippet: The total numbers of reads for all sgRNAs in each sample were normalized Figures were generated using the normalized read counts in R and RStudio (R project, Revolution Analytics), Matlab (Mathworks), and the Gene Set Enrichment Analysis tool (Broad Institute). .. Validation and Control Minipool Synthesis, Viral Transduction and In Vivo Transplantation Validation and control minipools were synthesized in a single oligonucleotide pool using a semiconductor-based electrochemical detritylation synthesis (CustomArray) and SAFC Proligo reagents (Sigma). ..

    Control:

    Article Title: CRISPR mediated in vivo modeling and genetic screening of tumor growth and metastasis
    Article Snippet: The total numbers of reads for all sgRNAs in each sample were normalized Figures were generated using the normalized read counts in R and RStudio (R project, Revolution Analytics), Matlab (Mathworks), and the Gene Set Enrichment Analysis tool (Broad Institute). .. Validation and Control Minipool Synthesis, Viral Transduction and In Vivo Transplantation Validation and control minipools were synthesized in a single oligonucleotide pool using a semiconductor-based electrochemical detritylation synthesis (CustomArray) and SAFC Proligo reagents (Sigma). ..

    Transduction:

    Article Title: CRISPR mediated in vivo modeling and genetic screening of tumor growth and metastasis
    Article Snippet: The total numbers of reads for all sgRNAs in each sample were normalized Figures were generated using the normalized read counts in R and RStudio (R project, Revolution Analytics), Matlab (Mathworks), and the Gene Set Enrichment Analysis tool (Broad Institute). .. Validation and Control Minipool Synthesis, Viral Transduction and In Vivo Transplantation Validation and control minipools were synthesized in a single oligonucleotide pool using a semiconductor-based electrochemical detritylation synthesis (CustomArray) and SAFC Proligo reagents (Sigma). ..

    In Vivo:

    Article Title: CRISPR mediated in vivo modeling and genetic screening of tumor growth and metastasis
    Article Snippet: The total numbers of reads for all sgRNAs in each sample were normalized Figures were generated using the normalized read counts in R and RStudio (R project, Revolution Analytics), Matlab (Mathworks), and the Gene Set Enrichment Analysis tool (Broad Institute). .. Validation and Control Minipool Synthesis, Viral Transduction and In Vivo Transplantation Validation and control minipools were synthesized in a single oligonucleotide pool using a semiconductor-based electrochemical detritylation synthesis (CustomArray) and SAFC Proligo reagents (Sigma). ..

    Transplantation Assay:

    Article Title: CRISPR mediated in vivo modeling and genetic screening of tumor growth and metastasis
    Article Snippet: The total numbers of reads for all sgRNAs in each sample were normalized Figures were generated using the normalized read counts in R and RStudio (R project, Revolution Analytics), Matlab (Mathworks), and the Gene Set Enrichment Analysis tool (Broad Institute). .. Validation and Control Minipool Synthesis, Viral Transduction and In Vivo Transplantation Validation and control minipools were synthesized in a single oligonucleotide pool using a semiconductor-based electrochemical detritylation synthesis (CustomArray) and SAFC Proligo reagents (Sigma). ..

    Synthesized:

    Article Title: CRISPR mediated in vivo modeling and genetic screening of tumor growth and metastasis
    Article Snippet: The total numbers of reads for all sgRNAs in each sample were normalized Figures were generated using the normalized read counts in R and RStudio (R project, Revolution Analytics), Matlab (Mathworks), and the Gene Set Enrichment Analysis tool (Broad Institute). .. Validation and Control Minipool Synthesis, Viral Transduction and In Vivo Transplantation Validation and control minipools were synthesized in a single oligonucleotide pool using a semiconductor-based electrochemical detritylation synthesis (CustomArray) and SAFC Proligo reagents (Sigma). ..



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