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5×sdspage loading buffer  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science 5×sdspage loading buffer
    5×Sdspage Loading Buffer, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sdspage+loading+buffer/5%C3%97sdspage+loading+buffer/pm39342142-92-69-72
    Average 90 stars, based on 1 article reviews
    5×sdspage loading buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Infection:

    Article Title: Identification of functional circRNAs regulating ovarian follicle development in goats.
    Article Snippet: At 72 h post-infection, the cells were processed following the guidelines of the Cell Cycle Assay Kit (Red Fluorescence) (Elabscience, China).After overnight incubation, the assay was performed on the machine and the red fluorescence at the excitation wavelength of 488 nm was recorded. .. Goat granulosa cells were spread in 6-well plates, and lentivirus infection was performed when the cells grew to 50%, and protein was extracted at 72 h. Firstly, the 6-well plate was placed on ice, washed with PBS, 200 μl RIPA Lysis Buffer (Beyotime, China) was added to each well, lysed on ice for 5 min, centrifuged at 4°C for 10 min, and the supernatant was aspirated and added to 5×SDSPAGE loading buffer (Solarbio, China) and boiled at 100°C for 10 min. .. The protein was separated by 10% AcrBis SDS-PAGE and transferred to polyvinylidene difluoride membrane (Merck, German), incubated with the corresponding primary antibody overnight (The PVDF membranes containing the target protein were cut into strips and then incubated with the primary antibody, using protein marks as a reference standard) and then added with the corresponding secondary antibody for 1 h. The protein bands were detected by chemiluminescent substrate.

    Lysis:

    Article Title: Identification of functional circRNAs regulating ovarian follicle development in goats.
    Article Snippet: At 72 h post-infection, the cells were processed following the guidelines of the Cell Cycle Assay Kit (Red Fluorescence) (Elabscience, China).After overnight incubation, the assay was performed on the machine and the red fluorescence at the excitation wavelength of 488 nm was recorded. .. Goat granulosa cells were spread in 6-well plates, and lentivirus infection was performed when the cells grew to 50%, and protein was extracted at 72 h. Firstly, the 6-well plate was placed on ice, washed with PBS, 200 μl RIPA Lysis Buffer (Beyotime, China) was added to each well, lysed on ice for 5 min, centrifuged at 4°C for 10 min, and the supernatant was aspirated and added to 5×SDSPAGE loading buffer (Solarbio, China) and boiled at 100°C for 10 min. .. The protein was separated by 10% AcrBis SDS-PAGE and transferred to polyvinylidene difluoride membrane (Merck, German), incubated with the corresponding primary antibody overnight (The PVDF membranes containing the target protein were cut into strips and then incubated with the primary antibody, using protein marks as a reference standard) and then added with the corresponding secondary antibody for 1 h. The protein bands were detected by chemiluminescent substrate.



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