loading control (OriGene)
90
Structured Review
OriGene
loading control
Loading Control, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sample+loading+kit/Loading+Control+Antibody+Sample+Kit/pm28084443-334-54-58
Average 90 stars, based on 4 article reviews
Loading Control, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sample+loading+kit/Loading+Control+Antibody+Sample+Kit/pm28084443-334-54-58
Average 90 stars, based on 4 article reviews
loading control - by Bioz Stars,
2026-09
90/100 stars
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Incubation:Article Title: Leucine-rich repeat kinase 2 controls protein kinase A activation state through phosphodiesterase 4. Article Snippet: .. Subsequently, membranes were incubated 1 h at room temperature (RT) with the following antibodies: rabbit anti-LRRK2 MJFF2 (1:500, Abcam #ab133474), rabbit anti-phospho S1292 LRRK2 (1:500, Abcam #ab203181), rabbit anti-p105/p50 (1:2000, Cell Signaling #13586S), rabbit anti-phospho S337 p50 (1:1000, Santa Cruz #101744), anti-flag HRP (1:20.000, Sigma Aldrich #A8592), anti-GFP (1:20.000, Roche #11814460001), mouse anti-PKA RIIβ (1:1000, BD Biosciences #610625), mouse anti-phospho S114 PKA RIIβ (1:1000, BD Biosciences #612550), goat anti-IL-1β (1:2000, R&D system #AF401NA), and Article Title: Leucine-rich repeat kinase 2 controls protein kinase A activation state through phosphodiesterase 4 Article Snippet: .. Subsequently, membranes were incubated 1 h at room temperature (RT) with the following antibodies: rabbit anti-LRRK2 MJFF2 (1:500, Abcam #ab133474), rabbit anti-phospho S1292 LRRK2 (1:500, Abcam #ab203181), rabbit anti-p105/p50 (1:2000, Cell Signaling #13586S), rabbit anti-phospho S337 p50 (1:1000, Santa Cruz #101744), anti-flag HRP (1:20.000, Sigma Aldrich #A8592), anti-GFP (1:20.000, Roche #11814460001), mouse anti-PKA RIIβ (1:1000, BD Biosciences #610625), mouse anti-phospho S114 PKA RIIβ (1:1000, BD Biosciences #612550), goat anti-IL-1β (1:2000, R&D system #AF401NA), and Article Title: Ablation of brainstem C1 neurons improves cardiac function in volume overload heart failure Article Snippet: David C. Andrade1,2,*, Camilo Toledo1,3,*, Hugo S. Dı́az1, Claudia Lucero1, Alexis Arce-Álvarez1,4, Luiz M. Oliveira5, Ana C. Takakura5, Thiago S. Moreira6, Harold D. Schultz7, Noah J. Marcus8, Julio Alcayaga9 and Rodrigo Del Rio1,3,10 1Laboratory of Cardiorespiratory Control, Department of Physiology, Pontificia Universidad Católica de Chile, Santiago, Chile; 2Centro de Investigación en Fisiologı́a del Ejercicio, Universidad Mayor, Santiago, Chile; 3Centro de Envejecimiento y Regeneración (CARE-UC), Pontificia Universidad Católica de Chile, Santiago, Chile; 4Escuela de Kinesiologı́a, Facultad de Salud, Universidad Católica Silva Henrı́quez, Santiago, Chile; 5Department of Pharmacology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, SP, Brasil; 6Department of Physiology and Biophysics, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, SP, Brasil; 7Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha NE, U.S.A.; 8Department of Physiology and Pharmacology, Des Moines University, Des Moines IA, U.S.A.; 9Laboratorio de Fisiologı́a Celular, Facultad de Ciencias, Universidad de Chile; 10Centro de Excelencia en Biomedicina de Magallanes (CEBIMA), Universidad de Magallanes, Punta Arenas, Chile SDS Page:Article Title: DOPAL derived alpha-synuclein oligomers impair synaptic vesicles physiological function Article Snippet: .. Protein was collected from the resin by adding 20 μl Laemmli buffer, loaded into 10% or 4–20% gradient SDS-PAGE and compared with the total lysate. aS detection was performed using antibodies against aS (Abcam, ab138501 for human cells and ab52168 for mouse neurons) and housekeeping proteins (beta-tubulin and HSP90) were detected using a loading control sample kit (OriGene, Article Title: DOPAL derived alpha-synuclein oligomers impair synaptic vesicles physiological function. Article Snippet: .. Protein was collected from the resin by adding 20 μ l Laemmli buffer, loaded into 10% or 4–20% gradient SDS-PAGE and compared with the total lysate. aS detection was performed using antibodies against aS (Abcam, ab138501 for human cells and ab52168 for mouse neurons) and housekeeping proteins (beta-tubulin and HSP90) were detected using a Control:Article Title: DOPAL derived alpha-synuclein oligomers impair synaptic vesicles physiological function Article Snippet: .. Protein was collected from the resin by adding 20 μl Laemmli buffer, loaded into 10% or 4–20% gradient SDS-PAGE and compared with the total lysate. aS detection was performed using antibodies against aS (Abcam, ab138501 for human cells and ab52168 for mouse neurons) and housekeeping proteins (beta-tubulin and HSP90) were detected using a loading control sample kit (OriGene, Article Title: Ablation of brainstem C1 neurons improves cardiac function in volume overload heart failure Article Snippet: David C. Andrade1,2,*, Camilo Toledo1,3,*, Hugo S. Dı́az1, Claudia Lucero1, Alexis Arce-Álvarez1,4, Luiz M. Oliveira5, Ana C. Takakura5, Thiago S. Moreira6, Harold D. Schultz7, Noah J. Marcus8, Julio Alcayaga9 and Rodrigo Del Rio1,3,10 1Laboratory of Cardiorespiratory Control, Department of Physiology, Pontificia Universidad Católica de Chile, Santiago, Chile; 2Centro de Investigación en Fisiologı́a del Ejercicio, Universidad Mayor, Santiago, Chile; 3Centro de Envejecimiento y Regeneración (CARE-UC), Pontificia Universidad Católica de Chile, Santiago, Chile; 4Escuela de Kinesiologı́a, Facultad de Salud, Universidad Católica Silva Henrı́quez, Santiago, Chile; 5Department of Pharmacology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, SP, Brasil; 6Department of Physiology and Biophysics, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, SP, Brasil; 7Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha NE, U.S.A.; 8Department of Physiology and Pharmacology, Des Moines University, Des Moines IA, U.S.A.; 9Laboratorio de Fisiologı́a Celular, Facultad de Ciencias, Universidad de Chile; 10Centro de Excelencia en Biomedicina de Magallanes (CEBIMA), Universidad de Magallanes, Punta Arenas, Chile Article Title: DOPAL derived alpha-synuclein oligomers impair synaptic vesicles physiological function. Article Snippet: .. Protein was collected from the resin by adding 20 μ l Laemmli buffer, loaded into 10% or 4–20% gradient SDS-PAGE and compared with the total lysate. aS detection was performed using antibodies against aS (Abcam, ab138501 for human cells and ab52168 for mouse neurons) and housekeeping proteins (beta-tubulin and HSP90) were detected using a Blocking Assay:Article Title: Ablation of brainstem C1 neurons improves cardiac function in volume overload heart failure Article Snippet: David C. Andrade1,2,*, Camilo Toledo1,3,*, Hugo S. Dı́az1, Claudia Lucero1, Alexis Arce-Álvarez1,4, Luiz M. Oliveira5, Ana C. Takakura5, Thiago S. Moreira6, Harold D. Schultz7, Noah J. Marcus8, Julio Alcayaga9 and Rodrigo Del Rio1,3,10 1Laboratory of Cardiorespiratory Control, Department of Physiology, Pontificia Universidad Católica de Chile, Santiago, Chile; 2Centro de Investigación en Fisiologı́a del Ejercicio, Universidad Mayor, Santiago, Chile; 3Centro de Envejecimiento y Regeneración (CARE-UC), Pontificia Universidad Católica de Chile, Santiago, Chile; 4Escuela de Kinesiologı́a, Facultad de Salud, Universidad Católica Silva Henrı́quez, Santiago, Chile; 5Department of Pharmacology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, SP, Brasil; 6Department of Physiology and Biophysics, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, SP, Brasil; 7Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha NE, U.S.A.; 8Department of Physiology and Pharmacology, Des Moines University, Des Moines IA, U.S.A.; 9Laboratorio de Fisiologı́a Celular, Facultad de Ciencias, Universidad de Chile; 10Centro de Excelencia en Biomedicina de Magallanes (CEBIMA), Universidad de Magallanes, Punta Arenas, Chile |
