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Precision Biomarker Resources total rna
Growth, aromatic intermediates production, and substrate consumption during batch fermentation cultures of E. coli PB12.SA22 strain grown in complex broth. (A) Biomass production (●) Glucose consumption (◆). (B) Residual Phenylalanine (☐), Tyrosine (○), Tryptophan (△). (C) SA (▲), DHS (◆), GA (▼) production. In panel A, numbers in parenthesis indicate the sampling time of biomass for total <t>RNA</t> extraction used <t>for</t> <t>microarray</t> analysis (1), (2) and (3) indicates samples collected at 5, 9, and 44 h of cultivation, respectively.
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1) Product Images from "Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium"

Article Title: Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium

Journal: Microbial Cell Factories

doi: 10.1186/1475-2859-13-28

Growth, aromatic intermediates production, and substrate consumption during batch fermentation cultures of E. coli PB12.SA22 strain grown in complex broth. (A) Biomass production (●) Glucose consumption (◆). (B) Residual Phenylalanine (☐), Tyrosine (○), Tryptophan (△). (C) SA (▲), DHS (◆), GA (▼) production. In panel A, numbers in parenthesis indicate the sampling time of biomass for total RNA extraction used for microarray analysis (1), (2) and (3) indicates samples collected at 5, 9, and 44 h of cultivation, respectively.
Figure Legend Snippet: Growth, aromatic intermediates production, and substrate consumption during batch fermentation cultures of E. coli PB12.SA22 strain grown in complex broth. (A) Biomass production (●) Glucose consumption (◆). (B) Residual Phenylalanine (☐), Tyrosine (○), Tryptophan (△). (C) SA (▲), DHS (◆), GA (▼) production. In panel A, numbers in parenthesis indicate the sampling time of biomass for total RNA extraction used for microarray analysis (1), (2) and (3) indicates samples collected at 5, 9, and 44 h of cultivation, respectively.

Techniques Used: Sampling, RNA Extraction, Microarray

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Article Snippet: The arrays were washed and stained by using standard Affymetrix protocols and scanned by using an Affymetrix GCS 3000 7G Scanner.

Article Title: Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium
Article Snippet: Samples were adjusted to a final concentration of total RNA = 1 μ g/ μ L. Aliquots of 20 μ L of total RNA were shipped to the Precision Biomarker company for microarray experiments ( http://www.precisionbiomarker.com ).

Article Title: Influence of RNA Labeling on Expression Profiling of MicroRNAs
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Microarray:

Article Title: Autonomous Vascular Networks Synchronize GABA Neuron Migration in the Embryonic Forebrain
Article Snippet: .. RNA was shipped to Precision Biomarker Resources Inc, Evanston, IL where RNA quality was determined and microarray hybridization was performed. .. Affymetrix’s Probe Logarithmic Intensity Error (PLIER) signal of “15” was used as the evidence of gene expression.

Article Title: BRD4 assists elongation of both coding and enhancer RNAs guided by histone acetylation
Article Snippet: Transcription was stopped with 20 mM EDTA, containing 200 mM NaCl, 1% SDS (w/v) and 25 μg glycogen and the G-less transcripts were then digested with 50 units RNAse T1 (Roche) for 30 minutes at 30°C. .. Microarray Amplification and labeling of cDNA and hybridization were carried out at Precision Biomarker Resources (Evanston, IL, USA). ..

Article Title: Derivation of a novel undifferentiated human foetal phenotype in serum-free cultures with BMP-2
Article Snippet: .. Microarray processing was conducted by Precision Biomarker Resources, Inc., (Evanston, IL, USA, http://www.precisionbiomarker.com ). .. Following confirmation of sample quality on a nanodrop ND-1000 (for optical densities at 230, 260 and 280) and a Bioanalyzer Nanochip (Agilent’s protocol), 1.0 μg of labelled cRNA from each sample was hybridized to Affymetrix U133 plus 2.0 chips in triplicate (giving three technical replicates per sample, with three biological replicates) and processed using the Affymetrix GeneChip Array Station (GCAS) protocol according to manufacturer’s instructions.

Hybridization:

Article Title: Autonomous Vascular Networks Synchronize GABA Neuron Migration in the Embryonic Forebrain
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Article Title: BRD4 assists elongation of both coding and enhancer RNAs guided by histone acetylation
Article Snippet: Transcription was stopped with 20 mM EDTA, containing 200 mM NaCl, 1% SDS (w/v) and 25 μg glycogen and the G-less transcripts were then digested with 50 units RNAse T1 (Roche) for 30 minutes at 30°C. .. Microarray Amplification and labeling of cDNA and hybridization were carried out at Precision Biomarker Resources (Evanston, IL, USA). ..

Amplification:

Article Title: BRD4 assists elongation of both coding and enhancer RNAs guided by histone acetylation
Article Snippet: Transcription was stopped with 20 mM EDTA, containing 200 mM NaCl, 1% SDS (w/v) and 25 μg glycogen and the G-less transcripts were then digested with 50 units RNAse T1 (Roche) for 30 minutes at 30°C. .. Microarray Amplification and labeling of cDNA and hybridization were carried out at Precision Biomarker Resources (Evanston, IL, USA). ..

Labeling:

Article Title: BRD4 assists elongation of both coding and enhancer RNAs guided by histone acetylation
Article Snippet: Transcription was stopped with 20 mM EDTA, containing 200 mM NaCl, 1% SDS (w/v) and 25 μg glycogen and the G-less transcripts were then digested with 50 units RNAse T1 (Roche) for 30 minutes at 30°C. .. Microarray Amplification and labeling of cDNA and hybridization were carried out at Precision Biomarker Resources (Evanston, IL, USA). ..



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