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rtl plus buffer  (Qiagen)


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  • 99

    Structured Review

    Qiagen rtl plus buffer
    Rtl Plus Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rtl+plus+buffer/RNeasy+Plus+Mini+Kit/pm41922576-99-7-10
    Average 99 stars, based on 5297 article reviews
    rtl plus buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    RNA Sequencing:

    Article Title: PLK1 inhibition by onvansertib enhances paclitaxel efficacy and overcomes resistance in HR+/HER2- breast cancer refractory to endocrine therapy and CDK4/6 inhibitors
    Article Snippet: Images were captured using an inverted fluorescence microscope (Olympus). .. 168 RNA-Sequencing 169 Cells were treated with onvansertib (25 nM), paclitaxel (5 nM), or their combination for 48 170 h. Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Cat No. 74136) and samples 171 with RNA integrity number (RIN) ≥8 were used for poly(A)+ mRNA library preparation. ..

    Isolation:

    Article Title: PLK1 inhibition by onvansertib enhances paclitaxel efficacy and overcomes resistance in HR+/HER2- breast cancer refractory to endocrine therapy and CDK4/6 inhibitors
    Article Snippet: Images were captured using an inverted fluorescence microscope (Olympus). .. 168 RNA-Sequencing 169 Cells were treated with onvansertib (25 nM), paclitaxel (5 nM), or their combination for 48 170 h. Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen, Cat No. 74136) and samples 171 with RNA integrity number (RIN) ≥8 were used for poly(A)+ mRNA library preparation. ..

    Article Title: Surveying alternative transcripts of disease-associated genes and their cell type specificity - a case study with ANK3.
    Article Snippet: Sequencing of full-length human and mouse ANK3 transcripts cDNA and long-range amplification We obtained RNA from human corpus callosum and frontal lobe (TakaraBio, #636133 and #636165). .. The same brain regions were dissected from mice and RNA was isolated using the RNeasy Plus Mini Kit (QIAGEN #74136). .. cDNA was synthesized using 1 μg total RNA, per brain region, using the SMARTer PCR cDNA Synthesis Kit (TakaraBio).

    Article Title: Inducible chronic NAD deficiency in mice reveals multi-systemic phenotypical, metabolic, and transcriptional changes
    Article Snippet: .. RNA was isolated from cells and mouse tissues using TRIzol (Invitrogen, 15596026) or the Qiagen RNeasy kit (Qiagen, 74136) according to the manufacturers’ instruction. .. cDNA was synthesized using the Qiagen QuantiTect or the ABI High-Capacity cDNA Reverse Transcription kit.

    other:

    Article Title: Pectin alleviates alcohol-associated liver disease partly through cell-type-specific aryl hydrocarbon receptor signaling in female mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Absolute ethanol Fisher Cat# 16606002 Pectin Sigma-Aldrich Cat# 93854 QIAzol lysis reagent Qiagen Cat# 79306 β-Mercaptoethanol Sigma-Aldrich Cat# M6250 Dithiothreitol Life technologies Cat# 707265ML dNTP mix Thermoscientific Cat# R0191 Oligo(dT) primer Merck Cat# 10814270001 M-MuLV reverse transcriptase Invitrogen Cat# 10338842 RNase inhibitor Merck Cat# 3335402001 SYBR Green master mix Roche Diagnostics Cat# 4887352001 Hoechst 33342 Molecular Probes/Life Technologies Cat# H-3570 Propidium iodide Thermo Fisher Scientific Cat# P3566 Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65–0866–18 Fixable Viability Dye eFluor 780 Thermo Fisher Scientific Cat# 65–0865–18 cOmplete Mini EDTA-free Protease Inhibitor Cocktail Roche Cat# 11836170001 PhosSTOP phosphatase inhibitor cocktail Merck Cat# 4906845001 Prestained Protein Ladder Plus Euromedex Cat# 06P-0211 PVDF membrane Millipore Cat# IPVH00010 Immobilon Western chemiluminescent HRP substrate Millipore Cat# WBKL50500 Sucrose Sigma-Aldrich Cat# S8501 OCT compound Sakura Finetek France SAS Cat# 4583 Triton X-100 Sigma-Aldrich Cat# T8787 BSA, IgG-free and protease-free Jackson ImmunoResearch Cat# 001–000–162 Donkey serum Jackson ImmunoResearch Cat# 017–000–121 Goat serum Jackson ImmunoResearch Cat# 005–000–121 DAPI Thermo Fisher Scientific Cat# D1306 Phalloidin Thermo Fisher Scientific Cat# A22287 Aqua-Poly/Mount Polysciences Cat# 18606–5 Critical commercial assays QIAamp Fast DNA Stool Mini Kit Qiagen Cat# 51604 RNeasy Lipid Tissue Mini Kit Qiagen Cat# 74804 RNeasy Plus Mini Kit Qiagen Cat# 74136 RNA 6000 Nano Kit Agilent Cat# 5067–1511 Custom U-PLEX Biomarker Group 1 (mouse) multiplex assay for IL-6, IL-10, and IL-22 Meso Scale Discovery Cat# K15069M-1 Lamina Propria Dissociation Kit, mouse Miltenyi Biotec Cat# 130–097–410 BD fixation/permeabilization reagents BD Biosciences Cat# 554722 Pierce BCA Protein Assay Kit Thermo Scientific Cat# 23225 Triglyceride Quantification Assay Kit Abcam Cat# ab65336 Oligonucleotides PCR1F_460 primer Eurogentec 5′-CTTTCCCTACACGACGCTCTT CCGATCTACGGRAGGCAGCAG-3′ PCR1R_460 primer Eurogentec 5′-GGAGTTCAGACGTGTGCTCTTCC GATCTTACCAGGGTATCTAATCCT-3′ Ahrflox genotyping primer oIMR6075 Eurogentec 5′-CAGTGGGAATAAGGCAAGAGTGA-3′ (Continued on next page) Cell Reports 45, 118085, October 27, 2026 15

    Article Title: Topoisomerase I inhibition yields a CD74 high /MHC high human microglial subtype with enhanced Aβ uptake.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER M-CSF R&D Cat.# 216-MC-010/CF EMEM ATCC Cat#: 30-2003 DMEM/F12 Thermo Fisher Cat.# 11320033 2X insulin-transferrin-selenite Thermo Fisher Cat.# 41400045 B27 Thermo Fisher Cat.# 17504044 N2 Thermo Fisher Cat.# 17502048 1X glutamax Thermo Fisher Cat.# 35050061 1X non-essential amino acids Thermo Fisher Cat.# 11140050 Monothioglycerol Sigma-Aldrich Cat.# M-6145 IL-34 R&D Cat.# 5265-IL-010/CF TGFβ1 STEMCELL Technologies Cat.# 100-0856 CD200 Bon Opus Biosciences Cat#: 50-203-1792 CX3CL1 R&D Systems Cat#: 365-FR-025/CF paraformaldehyde ThermoFisher Cat#: J61899-AP 20% Sucrose Invitrogen Cat#: 15503-022 ethylenediaminetetraacetic acid (EDTA) Sigma Cat#: ED4SS Triton X-100 Millipore Sigma, Cat#: 648466 aquamount Polysciences Inc. Cat#: 18606 RLT buffer Qiagen Cat#: 74136 2-Mercaptoethanol ThermoFisher Cat#:63689 P/S Gibco Cat#: 15140-122 Cytochalasin D Sigma-Aldrich Cat#: C8273 Trypsin Gen Clone Cat#:25-510F FCS Fisher Scientific Cat#: 10-438-026 Cell Staining buffer BioLegend Cat#: 420201 NaOH ThermoFisher Cat#: 63736.AK Critical commercial assays 10x Chromium Next GEM Single Cell 5′ Reagent Kits v2 (Dual Index) 10x Genomics, Pleasanton, CA Cat#: PN-1000263 TapeStation D5000 screening tapes and reagents Agilent, Santa Clara, CA Cat#: 5067-5588; 5067-5589 Qubit HS DNA quantification kit ThermoFisher Cat#: Q32851 STEMdiff Hematopoietic kit STEMCELL Technologies Cat#: 05310 STEMdiffTM Cerebral Organoid Kit STEMCELL Technologies Cat#: 08570, Cat#: 08571 MTT assay Invitrogen Cat#: M6494 Alamar Blue HS Thermo Fisher Cat#: DAL1100 Qiagen RNEasy mini plus kit Qiagen Cat#: 74136 BioRad iScript cDNA Synthesis kit BioRad Cat#:1708891 Qubit HS DNA quantification kit ThermoFisher Cat#: Q32851 Human Cytokine Proinflammatory Focused 15-Plex Discovery Assay Array (HDF15) Eve Technologies N/A Alamar Blue Assay ThermoFisher Cat#: DAL1025 Beta Amyloid (1–42), Aggregation Kit rPeptide A-1170-02 Deposited data AD Knowledge Portal: sc-RNA Sequencing HMC3 compound- treated https://www.synapse.org/Synapse: syn53124932 AD Knowledge Portal: sc-RNA Sequencing iMG compound- treated https://www.synapse.org/Synapse: syn53124932 (Continued on next page) 20 Cell Reports 45, 118031, October 27, 2026

    RNA Extraction:

    Article Title: Synergistic role of TFEB/TFE3 and mTORC1 in kidney cystogenesis and tumorigenesis.
    Article Snippet: Immunoreactive bands were detected by chemiluminescence and digitally acquired using an ImageQuant LAS4000 imaging system (GE Healthcare) or Amersham Imager 680 (Cytiva). .. RNA extraction, reverse transcription and quantitative PCR RNA samples from mouse kidneys were extracted using the RNeasy Plus Mini Kit (Qiagen cat no. 74136) according to the manufacturer’s instructions. .. cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen Cat no. / ID.

    Article Title: Leveraging high-throughput proteomics and AI-based protein folding to accelerate VAV1 molecular glue discovery.
    Article Snippet: .. RNA extraction, Reverse Transcription qPCR RNA for qPCR was extracted from cells using the RNeasy Plus Mini Kit (Qiagen, 74136) according to the manufacturer’s protocol. .. For quantification of VAV1 mRNA levels after treatment, expression of target genes was quantified using FlysisAmp Cells-to-CT 1-Step SYBR Green Kit (Vazyme, CL132-01).

    Reverse Transcription:

    Article Title: Synergistic role of TFEB/TFE3 and mTORC1 in kidney cystogenesis and tumorigenesis.
    Article Snippet: Immunoreactive bands were detected by chemiluminescence and digitally acquired using an ImageQuant LAS4000 imaging system (GE Healthcare) or Amersham Imager 680 (Cytiva). .. RNA extraction, reverse transcription and quantitative PCR RNA samples from mouse kidneys were extracted using the RNeasy Plus Mini Kit (Qiagen cat no. 74136) according to the manufacturer’s instructions. .. cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen Cat no. / ID.

    Article Title: Leveraging high-throughput proteomics and AI-based protein folding to accelerate VAV1 molecular glue discovery.
    Article Snippet: .. RNA extraction, Reverse Transcription qPCR RNA for qPCR was extracted from cells using the RNeasy Plus Mini Kit (Qiagen, 74136) according to the manufacturer’s protocol. .. For quantification of VAV1 mRNA levels after treatment, expression of target genes was quantified using FlysisAmp Cells-to-CT 1-Step SYBR Green Kit (Vazyme, CL132-01).

    Real-time Polymerase Chain Reaction:

    Article Title: Synergistic role of TFEB/TFE3 and mTORC1 in kidney cystogenesis and tumorigenesis.
    Article Snippet: Immunoreactive bands were detected by chemiluminescence and digitally acquired using an ImageQuant LAS4000 imaging system (GE Healthcare) or Amersham Imager 680 (Cytiva). .. RNA extraction, reverse transcription and quantitative PCR RNA samples from mouse kidneys were extracted using the RNeasy Plus Mini Kit (Qiagen cat no. 74136) according to the manufacturer’s instructions. .. cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen Cat no. / ID.

    Article Title: Leveraging high-throughput proteomics and AI-based protein folding to accelerate VAV1 molecular glue discovery.
    Article Snippet: .. RNA extraction, Reverse Transcription qPCR RNA for qPCR was extracted from cells using the RNeasy Plus Mini Kit (Qiagen, 74136) according to the manufacturer’s protocol. .. For quantification of VAV1 mRNA levels after treatment, expression of target genes was quantified using FlysisAmp Cells-to-CT 1-Step SYBR Green Kit (Vazyme, CL132-01).



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