Review



onestep rt pcr buffer  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Qiagen onestep rt pcr buffer
    Onestep Rt Pcr Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 11280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+buffer/QIAGEN+OneStep+Ahead+RT-PCR+Kit/pm41316685-66-45-50
    Average 99 stars, based on 11280 article reviews
    onestep rt pcr buffer - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    One Step RT-PCR:

    Article Title: Isolation and molecular characterization of peste des petits ruminants' virus in small ruminants from outbreak cases in Eastern Ethiopia.
    Article Snippet: The amplification was performed with the final reaction volume of 20μl containing 17μl of the prepared master mix and 3μl of RNA template at 50°C for 15 min, 95°C for 20 sec, followed by 45 cycles of denaturation and annealing at 95°C for 30 sec and extension at 60°C for 30 sec in Real-time Applied Bio systems 7500 thermal cycler PCR machine [26]. .. The one-step RT PCR kit (Qiagen, Germany); forward primer: NP3 (5′ GTC TCG GAA ATC GCC TCA CAG ACT 3′) and reverse primer NP4 (5′ CCT CCT CCT GGT CCT CCA GAA TCT 3′) were used. ..

    Article Title: Co-circulation of multiple arboviruses in acute febrile patients in Yunnan, China, identified by metagenomic sequencing
    Article Snippet: .. The first-round amplification was conducted using the One-Step RT-PCR Kit (QIAGEN, Germany), according to the manufacturer’s protocol. .. The second round of PCR was carried out using Premix Taq (Takara, Japan) with a reaction mixture consisting of 1 μL of the first-round PCR product as the template, 1 μL each of the forward and reverse primers, 25 μL of Premix Taq, and 22 μL of nuclease-free water.

    Article Title: Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Interlaboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability.
    Article Snippet: .. Five participants used the QIAGEN QIAamp Viral RNA Mini Kit with the QIAGEN One-Step RT-PCR Kit, and 8 used the VetMax Gold kit. ..

    Article Title: Influenza A and B Viral Shedding in Adults Immunized with Live Attenuated Influenza Vaccine.
    Article Snippet: .. Reactions were performed using the QuantiTect One-Step RT-PCR Kit (Qiagen) on the LightCycler® 480 II (Roche). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Emergence and molecular characterization of a resistance-breaking tomato spotted wilt virus isolate on bell pepper plants harboring the Tsw resistance gene in Japan.
    Article Snippet: western flower thrips) in a circulative propagative transmission manner.. TSWV has a broad host range and infects >500 plant species, belonging to the families Solanaceae, Cucurbitaceae, and Asteraceae [2], causing yellow spotted wilt disease in plants, such as tomatoes and bell peppers.. The TSWV genome consists of three negative-strand RNAs, named L, M, and S, which vary in length.

    Article Title: Composition of NY-ESO-1-specific t cell receptors restricted on multiple major histocompatibility complex molecules
    Article Snippet: .. Novel TCR variable genes were cloned from single, sorted T cells using a custom panel of human TCR variable region-specific primers with the Qiagen OneStep RT-PCR kit (Redwood City, CA), followed by a nested PCR amplification step. ..

    Article Title: Molecular Surveillance of Coronaviruses in Riyadh (2025-2026): Persistent Genotype C and Conserved N-Glycosylation Motifs in Human Coronavirus OC43.
    Article Snippet: Viral RNA was extracted from the NPA samples using the QIAamp viral RNA extraction kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Initial screening for the four common HCoVs (HCoV-OC43, -NL63, -HKU1, and -229E) was performed using the OneStep RT-PCR kit (Qiagen). ..

    Article Title: The microprotein SEP 53BP1 : its bizarre mode of translational expression and intracellular behaviour
    Article Snippet: It was treated with DNase (DNA free; Ambion), and quality was checked on an Agilent 2100 bioanalyzer. .. Semi-quantitative RT-PCR was performed starting with 100 ng of total cell RNA using the QIAGEN OneStep RT-PCR Kit (in the RT minus control samples were heated to 95°C for 5 mins prior to starting. ..

    Isolation:

    Article Title: Emergence and molecular characterization of a resistance-breaking tomato spotted wilt virus isolate on bell pepper plants harboring the Tsw resistance gene in Japan.
    Article Snippet: western flower thrips) in a circulative propagative transmission manner.. TSWV has a broad host range and infects >500 plant species, belonging to the families Solanaceae, Cucurbitaceae, and Asteraceae [2], causing yellow spotted wilt disease in plants, such as tomatoes and bell peppers.. The TSWV genome consists of three negative-strand RNAs, named L, M, and S, which vary in length.

    Amplification:

    Article Title: Co-circulation of multiple arboviruses in acute febrile patients in Yunnan, China, identified by metagenomic sequencing
    Article Snippet: .. The first-round amplification was conducted using the One-Step RT-PCR Kit (QIAGEN, Germany), according to the manufacturer’s protocol. .. The second round of PCR was carried out using Premix Taq (Takara, Japan) with a reaction mixture consisting of 1 μL of the first-round PCR product as the template, 1 μL each of the forward and reverse primers, 25 μL of Premix Taq, and 22 μL of nuclease-free water.

    Article Title: Composition of NY-ESO-1-specific t cell receptors restricted on multiple major histocompatibility complex molecules
    Article Snippet: .. Novel TCR variable genes were cloned from single, sorted T cells using a custom panel of human TCR variable region-specific primers with the Qiagen OneStep RT-PCR kit (Redwood City, CA), followed by a nested PCR amplification step. ..

    Clone Assay:

    Article Title: Composition of NY-ESO-1-specific t cell receptors restricted on multiple major histocompatibility complex molecules
    Article Snippet: .. Novel TCR variable genes were cloned from single, sorted T cells using a custom panel of human TCR variable region-specific primers with the Qiagen OneStep RT-PCR kit (Redwood City, CA), followed by a nested PCR amplification step. ..

    Nested PCR:

    Article Title: Composition of NY-ESO-1-specific t cell receptors restricted on multiple major histocompatibility complex molecules
    Article Snippet: .. Novel TCR variable genes were cloned from single, sorted T cells using a custom panel of human TCR variable region-specific primers with the Qiagen OneStep RT-PCR kit (Redwood City, CA), followed by a nested PCR amplification step. ..

    Control:

    Article Title: The microprotein SEP 53BP1 : its bizarre mode of translational expression and intracellular behaviour
    Article Snippet: It was treated with DNase (DNA free; Ambion), and quality was checked on an Agilent 2100 bioanalyzer. .. Semi-quantitative RT-PCR was performed starting with 100 ng of total cell RNA using the QIAGEN OneStep RT-PCR Kit (in the RT minus control samples were heated to 95°C for 5 mins prior to starting. ..



    Similar Products

    96
    LI-COR rt 1 2000 in array buffer 5 li cor 926 32230
    Rt 1 2000 In Array Buffer 5 Li Cor 926 32230, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+buffer/IRDye+800CW+STREPTAVIDIN/pm42014405-584-20-26
    Average 96 stars, based on 1 article reviews
    rt 1 2000 in array buffer 5 li cor 926 32230 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    99
    TaKaRa rt pcr buffer
    Rt Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+buffer/Premix+Ex+Taq/10__3390_slash_jof12040282-85-12-22
    Average 99 stars, based on 1 article reviews
    rt pcr buffer - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    10X Genomics rt buffer b
    Rt Buffer B, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+buffer/pm42260119-394-14-17
    Average 86 stars, based on 1 article reviews
    rt buffer b - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    94
    Thermo Fisher rt buffer
    Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+buffer/TRIS%2C+1%2E0M+buffer+soln%2E%2C+pH+8%2E4/pm42123321-240-56-85
    Average 94 stars, based on 1 article reviews
    rt buffer - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher rt hybridisation buffer
    Rt Hybridisation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+buffer/Tween+20/bio_rxiv__64898__2026__04__16__718949-182-93-123
    Average 99 stars, based on 1 article reviews
    rt hybridisation buffer - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    94
    Bio-Rad lysis buffer
    Lysis Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+buffer/iScript+RT-qPCR+Sample+Preparation+Reagent/pmc13061224-241-13-20
    Average 94 stars, based on 1 article reviews
    lysis buffer - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    99
    Qiagen onestep rt pcr buffer
    Onestep Rt Pcr Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+buffer/QIAGEN+OneStep+Ahead+RT-PCR+Kit/pm41316685-66-45-50
    Average 99 stars, based on 1 article reviews
    onestep rt pcr buffer - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results