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Bioer Technology Co Ltd 5x rt buffer
5x Rt Buffer, supplied by Bioer Technology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt+buffer/5x+rt+buffer/pm23708057-70-38-41
Average 90 stars, based on 1 article reviews
5x rt buffer - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Reverse Transcription:

Article Title: Feasibility and effect of ultrasound microbubble-mediated wild-type p53 gene transfection of HeLa cells
Article Snippet: The quantity of RNA isolates was determined spectrophotometrically using a DNA/RNA Gene-Quant Calculator (Amersham Biosciences, USA). .. Reverse transcription was performed in 20 μl of reaction mixture containing 2 μg of total RNA, 5.0 units of AMV reverse transcriptase, 50 pmol of oligo(dT) primer, 40 nmol of dNTP mixture, 40 units of RNase inhibitor, 4 μl of 5X RT buffer (Bioer, Hangzhou, China) at 42°C for 1 h and 95°C for 5 min. ..

Article Title: Tubeimoside-1 induces G2/M phase arrest and apoptosis in SKOV-3 cells through increase of intracellular Ca²⁺ and caspase-dependent signaling pathways.
Article Snippet: The quantity of RNA isolates was determined spectrophotometrically using a DNA/RNA Gene-Quant Calculator (Amersham Biosciences, USA). .. Reverse transcription was performed in 20 μl of reaction mixture containing 2 μg of total RNA, 5.0 units of AMV reverse transcriptase, 50 pmol of oligo-dT primer, 40 nmol of dNTP mixture, 40 units of RNase inhibitor, 4 μl of 5X RT buffer (Bioer, Hangzhou, China) at 42 ̊C for 1 h and 95 ̊C for 5 min. ..

other:

Article Title: Chitosan oligosaccharides protect human umbilical vein endothelial cells from hydrogen peroxide-induced apoptosis
Article Snippet: 0144-8617/$ see front matter 2010 Elsevier Ltd. A doi:10.1016/j.carbpol.2010.01.025 * Corresponding authors.. Address: Laboratory of Engineering Research, Dalian Institute of Chemical P Road, Dalian 116023, PR China.. Tel.

Article Title: Genetic, transcriptional, and regulatory landscape of monolignol biosynthesis pathway in Miscanthus × giganteus
Article Snippet: The mixture was added with 5 μl of 5× M-MLV buffer, 2 μl of RNase-free ddH 2 O, 1 μl of M-MLV Reverse Transcriptase and then incubated on a LifePro Thermal Cycler (BIOER, Hangzhou, China) at 42 °C for 1.5 h. For quantitative PCR (qPCR) experiments, the cDNAs were synthesized using a FastQuant RT Kit (KR106, TIANGEN Biotech).

Article Title: Effect of Ammonia-Oxidizing Bacterial Strains That Coexist in Rhizosphere Soil on Italian Ryegrass Regrowth.
Article Snippet: Each 25-μL PCR reaction mixture included 2.5 μL of 10× buffer (with Mg2+), 2 μL of dNTP (2.5 mmol·L−1), 0.4 μL each of forward and reverse primers (10 μmol·L−1), 30 ng of DNA template, and 0.75 U of Ex Taq DNA polymerase (Bioer Technology Co., Ltd., Hangzhou, China).

Synthesized:

Article Title: The mucin-type glycosylating enzyme polypeptide N-acetylgalactosaminyltransferase 14 promotes the migration of ovarian cancer by modifying mucin 13.
Article Snippet: The quantity of RNA samples was measured by UV absorbance at 260-280 nm using a DNA/RNA GeneQuant Calculator (Amersham Biosciences, Piscataway, NJ, USA). .. Total cDNA was synthesized from 2 μg total RNA in 20 μl of reaction mixture containing 50 pmol of oligo-dT, 5.0 units of AMV reverse transcriptase, 40 units of RNase inhibitor, 40 nmol of dNTP, 4 μl of 5X RT buffer (Bioer, Hangzhou, China). ..

Amplification:

Article Title: Injectable body temperature responsive hydrogel for encephalitis treatment via sustained release of nano-anti-inflammatory agents
Article Snippet: Furthermore, the synthesis of cDNA was performed following the guidelines of the reverse transcription kit (BioSharp). .. A precise mixture of the RNA template, SuperRT III All-in-one RT Mix, SuperRT III All-in-one RT Buffer, and Nuclease-free Water was prepared and utilised to synthesize cDNA in a gene amplification instrument (Gene Explorer, Bioer Technology, Hangzhou, Zhejiang, China). .. Subsequently, following the protocol of the universal quantitative polymerase chain reaction (qPCR) kit (BioSharp), a proportional mix of the cDNA template, 2× Universal SYBR qPCR Mix, primers (Sangon Biotech Co., Ltd., Shanghai, China), ROX Reference Dye, and RNase-Free ddH 2 O was prepared and subjected to real-time PCR analysis using a Real-time PCR amplification (Applied Biosystems QuantStudio 5 instrument, Thermo Fisher Scientific).



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