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Pathogenomix Inc ripseq ngs software
Ripseq Ngs Software, supplied by Pathogenomix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ripseq+software/ripseq+ngs+software/pmc08803847-210-7-10
Average 90 stars, based on 1 article reviews
ripseq ngs software - by Bioz Stars, 2026-10
90/100 stars

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Next-Generation Sequencing:

Article Title: Pleural space infection microbiology as assessed using a clinical sequencing-based assay: Fusobacterium nucleatum group, Streptococcus intermedius, and other oral normal microbiota are the most common bacteria identified in community-acquired pleural space infections.
Article Snippet: The definition of the microbiology of pleural space infection has been challenging due to the poor yield of conventional culture.. Here, the results of a 16S ribosomal RNA gene PCR/sequencing assay performed on pleural fluid in routine clinical practice between August 2020 and January 2023 were evaluated.. Amplified 16S rRNA gene DNA was submitted to Sanger sequencing and/or next-generation sequencing or results were reported as negative, depending on PCR crossing threshold value.

Article Title: Pathogen Detection in Infective Endocarditis Using Targeted Metagenomics on Whole Blood and Plasma: a Prospective Pilot Study.
Article Snippet: Paired-end sequencing was done on an Illumina MiSeq using a 2 300 cycle V3 Nano kit (Illumina), followed by onboard Illumina processing (adapter trimming, index demultiplexing, and fastq generation). .. For bioinformatic analysis, files were processed using Pathogenomix RipSeq NGS software that uses the “Pathogenomix Prime 16S database” for de novo clustering into Operational Taxonomic Units (OTUs) using a 99% homology threshold, as described in the Supplemental Methods. ..

Article Title: The Brief Case: Human leptospirosis acquired in Minnesota and diagnosed using 16S ribosomal RNA gene PCR/next-generation sequencing of blood
Article Snippet: A 72-year-old man from rural Minnesota presented to the emergency department in November 2023 with a 4-day history of fever, chills, vomiting, diarrhea, cough, and shortness of breath.. He had underlying sarcoidosis, bronchiectasis, paroxysmal atrial fibrillation, and sinus bradycardia and had a pacemaker in place.. He reported extensive zoonotic exposures in Minnesota during the 2 weeks before presentation, including deer hunting until 5 days before the symptom onset, consumption of cooked game meat, and freshwater exposure.

Article Title: Pleural Empyema Caused by Streptococcus intermedius and Fusobacterium nucleatum: A Distinct Entity of Pleural Infections.
Article Snippet: .. For sequence data analysis, we used the RipSeq NGS software (Pathogenomix, Santa Cruz, CA, USA) [2]. ..

Article Title: Comparison of the BioFire Joint Infection Panel to 16S Ribosomal RNA Gene-Based Targeted Metagenomic Sequencing for Testing Synovial Fluid from Patients with Knee Arthroplasty Failure.
Article Snippet: Onboard Illumina processing included adapter trimming, index demultiplexing, and fastq generation. .. Files were processed using Pathogenomix RipSeq NGS software (Santa Cruz, CA). ..

Article Title: Targeted Metagenomic Sequencing-based Approach Applied to 2146 Tissue and Body Fluid Samples in Routine Clinical Practice.
Article Snippet: Bidirectional Sanger sequencing was performed on an Applied Biosystems 3500xl instrument (Thermo Fisher Scientific), and NGS was performed on an Illumina Miseq with a 500-cycle (2 × 250 paired-end read) v2 nano kit. .. Bioinformatic analysis was performed with RipSeq NGS software (Pathogenomix; Supplementary Methods). ..

Article Title: Clinical Use of a 16S Ribosomal RNA Gene-Based Sanger and/or Next Generation Sequencing Assay to Test Preoperative Synovial Fluid for Periprosthetic Joint Infection Diagnosis
Article Snippet: An applied Biosystems 3500xl instrument (Thermo Fisher Scientific) was used for Sanger sequencing and an Illumina MiSeq with a 500 cycle (2 × 250 paired-end read) v2 nano kit for NGS. .. Sequencing results were processed with RipSeq NGS software (Pathogenomix, CA), as previously described ( ). ..

Article Title: Pleural Empyema Caused by Streptococcus intermedius and Fusobacterium nucleatum : A Distinct Entity of Pleural Infections
Article Snippet: .. For sequence data analysis, we used the RipSeq NGS software (Pathogenomix, Santa Cruz, CA, USA) [ ]. ..

Software:

Article Title: Pleural space infection microbiology as assessed using a clinical sequencing-based assay: Fusobacterium nucleatum group, Streptococcus intermedius, and other oral normal microbiota are the most common bacteria identified in community-acquired pleural space infections.
Article Snippet: The definition of the microbiology of pleural space infection has been challenging due to the poor yield of conventional culture.. Here, the results of a 16S ribosomal RNA gene PCR/sequencing assay performed on pleural fluid in routine clinical practice between August 2020 and January 2023 were evaluated.. Amplified 16S rRNA gene DNA was submitted to Sanger sequencing and/or next-generation sequencing or results were reported as negative, depending on PCR crossing threshold value.

Article Title: Pathogen Detection in Infective Endocarditis Using Targeted Metagenomics on Whole Blood and Plasma: a Prospective Pilot Study.
Article Snippet: Paired-end sequencing was done on an Illumina MiSeq using a 2 300 cycle V3 Nano kit (Illumina), followed by onboard Illumina processing (adapter trimming, index demultiplexing, and fastq generation). .. For bioinformatic analysis, files were processed using Pathogenomix RipSeq NGS software that uses the “Pathogenomix Prime 16S database” for de novo clustering into Operational Taxonomic Units (OTUs) using a 99% homology threshold, as described in the Supplemental Methods. ..

Article Title: Pleural Empyema Caused by Streptococcus intermedius and Fusobacterium nucleatum: A Distinct Entity of Pleural Infections.
Article Snippet: .. For sequence data analysis, we used the RipSeq NGS software (Pathogenomix, Santa Cruz, CA, USA) [2]. ..

Article Title: Comparison of the BioFire Joint Infection Panel to 16S Ribosomal RNA Gene-Based Targeted Metagenomic Sequencing for Testing Synovial Fluid from Patients with Knee Arthroplasty Failure.
Article Snippet: Onboard Illumina processing included adapter trimming, index demultiplexing, and fastq generation. .. Files were processed using Pathogenomix RipSeq NGS software (Santa Cruz, CA). ..

Article Title: Targeted Metagenomic Sequencing-based Approach Applied to 2146 Tissue and Body Fluid Samples in Routine Clinical Practice.
Article Snippet: Bidirectional Sanger sequencing was performed on an Applied Biosystems 3500xl instrument (Thermo Fisher Scientific), and NGS was performed on an Illumina Miseq with a 500-cycle (2 × 250 paired-end read) v2 nano kit. .. Bioinformatic analysis was performed with RipSeq NGS software (Pathogenomix; Supplementary Methods). ..

Article Title: Clinical Use of a 16S Ribosomal RNA Gene-Based Sanger and/or Next Generation Sequencing Assay to Test Preoperative Synovial Fluid for Periprosthetic Joint Infection Diagnosis
Article Snippet: An applied Biosystems 3500xl instrument (Thermo Fisher Scientific) was used for Sanger sequencing and an Illumina MiSeq with a 500 cycle (2 × 250 paired-end read) v2 nano kit for NGS. .. Sequencing results were processed with RipSeq NGS software (Pathogenomix, CA), as previously described ( ). ..

Article Title: Pleural Empyema Caused by Streptococcus intermedius and Fusobacterium nucleatum : A Distinct Entity of Pleural Infections
Article Snippet: .. For sequence data analysis, we used the RipSeq NGS software (Pathogenomix, Santa Cruz, CA, USA) [ ]. ..

Amplification:

Article Title: The Brief Case: Human leptospirosis acquired in Minnesota and diagnosed using 16S ribosomal RNA gene PCR/next-generation sequencing of blood
Article Snippet: A 72-year-old man from rural Minnesota presented to the emergency department in November 2023 with a 4-day history of fever, chills, vomiting, diarrhea, cough, and shortness of breath.. He had underlying sarcoidosis, bronchiectasis, paroxysmal atrial fibrillation, and sinus bradycardia and had a pacemaker in place.. He reported extensive zoonotic exposures in Minnesota during the 2 weeks before presentation, including deer hunting until 5 days before the symptom onset, consumption of cooked game meat, and freshwater exposure.

Sequencing:

Article Title: Pleural Empyema Caused by Streptococcus intermedius and Fusobacterium nucleatum: A Distinct Entity of Pleural Infections.
Article Snippet: .. For sequence data analysis, we used the RipSeq NGS software (Pathogenomix, Santa Cruz, CA, USA) [2]. ..

Article Title: Clinical Use of a 16S Ribosomal RNA Gene-Based Sanger and/or Next Generation Sequencing Assay to Test Preoperative Synovial Fluid for Periprosthetic Joint Infection Diagnosis
Article Snippet: An applied Biosystems 3500xl instrument (Thermo Fisher Scientific) was used for Sanger sequencing and an Illumina MiSeq with a 500 cycle (2 × 250 paired-end read) v2 nano kit for NGS. .. Sequencing results were processed with RipSeq NGS software (Pathogenomix, CA), as previously described ( ). ..

Article Title: Pleural Empyema Caused by Streptococcus intermedius and Fusobacterium nucleatum : A Distinct Entity of Pleural Infections
Article Snippet: .. For sequence data analysis, we used the RipSeq NGS software (Pathogenomix, Santa Cruz, CA, USA) [ ]. ..



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(a) Alignment of the partial 16S rRNA gene sequence from the case isolate of F. sanguinis with F. sanguinis strain CCUG 47711 chromosome, complete genome (sequence ID: CP027226). There are two mismatches at position 202 (G to R=A or G) and at position 439 (A to R) resulting in a match of 99.5 %. (b) Alignment of the sequence obtained from the mixed sequence from the case isolate using <t>the</t> <t>Pathogenomix</t> <t>Ripseq</t> software and database, of M. indoligenes with M. indoligenes strain MDA2477 (sequence ID: AF527773). There are several mismatches and gaps, resulting in a match of 95.4 %.
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(a) Alignment of the partial 16S rRNA gene sequence from the case isolate of F. sanguinis with F. sanguinis strain CCUG 47711 chromosome, complete genome (sequence ID: CP027226). There are two mismatches at position 202 (G to R=A or G) and at position 439 (A to R) resulting in a match of 99.5 %. (b) Alignment of the sequence obtained from the mixed sequence from the case isolate using the Pathogenomix Ripseq software and database, of M. indoligenes with M. indoligenes strain MDA2477 (sequence ID: AF527773). There are several mismatches and gaps, resulting in a match of 95.4 %.

Journal: Access Microbiology

Article Title: Bacteraemia with Moryella indoligenes and Fastidiosipila sanguinis : a case report

doi: 10.1099/acmi.0.000108

Figure Lengend Snippet: (a) Alignment of the partial 16S rRNA gene sequence from the case isolate of F. sanguinis with F. sanguinis strain CCUG 47711 chromosome, complete genome (sequence ID: CP027226). There are two mismatches at position 202 (G to R=A or G) and at position 439 (A to R) resulting in a match of 99.5 %. (b) Alignment of the sequence obtained from the mixed sequence from the case isolate using the Pathogenomix Ripseq software and database, of M. indoligenes with M. indoligenes strain MDA2477 (sequence ID: AF527773). There are several mismatches and gaps, resulting in a match of 95.4 %.

Article Snippet: Using the Pathogenomix Ripseq software and database the best hits were F. sanguinis with a score of 100, and a Lachnospiraceae species with a score of 99.5 [ ].

Techniques: Sequencing, Software