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algorithms schrödinger suite  (Addgene inc)


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    Structured Review

    Addgene inc algorithms schrödinger suite
    Algorithms Schrödinger Suite, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhon+algorithm/YF-RhoA(CA)+(Plasmid+%2320153)/pm30917327-210-62-58
    Average 91 stars, based on 4 article reviews
    algorithms schrödinger suite - by Bioz Stars, 2026-11
    91/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Polycystin-1 regulates ARHGAP35-dependent centrosomal RhoA activation and ROCK signaling
    Article Snippet: Cilia were stained with Arl13b and measurements were performed on mCherry-PC1–transfected cells with an Olympus Imaging Systems inverted IX-71 microscope set to capture cellular fluorescence images with a CCD camera (Hamamatsu), driven by Simple PCI software (C Imaging Systems). .. YF-RhoA (CA) (Addgene plasmid 20153) and Lyn11-targeted FRB (LDR) (Addgene plasmid 20147) were gifts of Tobias Meyer, Stanford University, Stanford, California, USA ( ). ..

    Article Title: Tim4 Recognizes Carbon Nanotubes and Mediates Frustrated Phagocytosis Leading to Granuloma Formation
    Article Snippet: 1Graduate School of Information Sciences, Tohoku University, Sendai, Japan 2Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan 3Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Sendai, Japan 4Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, Japan 5Laboratory of Immunology and Microbiology, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Japan 6Department of Cancer Biology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan 7JST, PRESTO, Kawaguchi, Japan 8Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan 9Laboratory of Bioresponse Regulation, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan 10Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan 11Laboratory of Biochemistry and Immunology, Immunology Frontier Research Center, Osaka University, Suita, Japan 12Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan 13Lead Contact Email: mnakayam@fc.ritsumei.ac.jp; kengo@ecei.tohoku.ac.jp

    Article Title: Discovery of Small Molecules that Activate RNA Methylation through Cooperative Binding to the METTL3-14-WTAP Complex Active Site.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Polyclonal rabbit-anti-m6A antibody Synaptic systems Cat#202 003; RRID:AB_2279214 Goat-anti-rabbit-IRDye 800CW LI-COR Biosciences Cat#926-32211; RRID:AB_621843 Goat-anti-mouse-IRDye 680LT LI-COR Biosciences RRID:AB_10706161 Monoclonal mouse-anti-FLAG M2 Sigma-Aldrich Cat#F1804; RRID:AB_262044 Polyclonal rabbit-anti-METTL3 Sigma-Aldrich Cat# HPA001299; RRID:AB_1079419 Polyclonal rabbit-anti-METTL14 Sigma-Aldrich Cat#HPA038002; RRID:AB_10672401 Monoclonal mouse-anti-WTAP Sigma-Aldrich Cat#SAB1402479, RRID:AB_10639633 Chemicals, Peptides, and Recombinant Proteins Methyl piperidine-3-carboxylate hydrochloride ArkPharm, Inc. AK-86479 Tert-butyl 6-methylpiperidine-3-carboxylate Life Chemicals Inc. F2163-0155 Methyl 6-methylpiperidine-3-carboxylate ArkPharm, Inc. AK103663 Methyl piperazine-2-carboxylate ChemDiv, Inc. FF20-0374 Reagent 007 for chemical transfection Icosagen, Ltd. Cat#R007-P010 EX-CELL 420 Serum-Free Medium Merck Cat#14420C InSolution Sinefungin Millipore Cat#567051 Propidium Iodide Invitrogen Cat#P3566 TRIzol Invitrogen Cat#15596018 Meclofenamate (sodium salt) Cayman chemicals Cat#70550 Nuclease P1 FUJIFILM Wako Chemicals Europe Cat#145-08221 Alkaline phosphatase Roche Cat#10713023001 Lipofectamine 2000 Ivitrogen Cat#11668019 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 3X FLAG Peptide Sigma-Aldrich Cat#F4799 RNaseOUT Recombinant Ribonuclease Inhibitor Ivitrogen Cat#10777019 Tritiated S-(5-adenosyl)-L–methionine Perkin Elmer Cat#NET155V250UC S-(5-Adenosyl)-L-methionine chloride dihydrochloride Sigma-Aldrich Cat#A7007 S-(5-Adenosyl)-L-homocysteine Sigma-Aldrich Cat#A9384 N6-methyladenosine (m6A) Selleckchem Cat#S3190 Precision Plus Protein Dual Color Standard BIO-RAD Cat#161-0374 Odyssey Blocking Buffer (PBS) LI-COR Biosciences Cat#927-40000 Adenosine Sigma-Aldrich Cat#A9251 Ammonium formate Fluka Cat#55674 Methanol (MeOH) HiPerSolv N/A Critical Commercial Assays PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems Series S Sensor Chip CM5 Series S Sensor Chip CM5 Series S Sensor Chip CM5 Amine Coupling Kit GE Healthcare Bio-Sciences Cat#BR-1000-50 Experimental Models: Cell Lines HEK293 ATCC CRL-1573 Sf9 Cell Line from Spodoptera frugiperda ThermoFisher Cat # 11496015 (Continued on next page) e1 Cell Reports 26, 3762–3771.e1–e5, March 26, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides 50-uacacucgaucuggacuaaagcugcuc-biotin-30 Li et al., 2016, Integrated DNA Technologies N/A Recombinant DNA Plasmid: 1xmut1 (METTL3/UniProt: Q86U44/ W373A) This paper N/A Plasmid: 1xmut2 (METTL3/UniProt: Q86U44/ D395A) This paper N/A Plasmid: 2xmut (METTL3 /UniProt: Q86U44/ W373A; K513A) This paper N/A pFastBac1 ThermoFisher N/A pcDNA3/Flag-METTL3 Liu et al., 2014, Addgene RRID:Addgene_53739 pcDNA3/Flag-METTL14 Liu et al., 2014, Addgene RRID:Addgene_53740 Software and Algorithms Schrödinger Suite 2015-3 Schrödinger LLC RRID:SCR_014879 Maestro 10.7 Schrödinger LLC RRID:SCR_016748 Desmond 3.6.0.0 Schrödinger LLC RRID:SCR_014575 AutoDock Tools 1.5.6 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 AutoDock 4.2 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 GraphPad prism 7 GraphPad Software / RRID:SCR_015807 ImageJ Schindelin et al., 2012 RRID:SCR_003070 BD Accuri C6 Plus BD Biosciences RRID:SCR_014422 Biacore T100 Control Software v. 2.0.3 GE Healthcare N/A Biacore T100 Evaluation Software v. 2.0.3 GE Healthcare N/A Other Millex Syringe Filter, Durapore (PVDF) Millipore Cat #SLGVR04NL FlashPlate Plus, Streptavidin Perkin Elmer Cat #SMP103A001PK 4–20% Mini-PROTEAN TGX Precast Protein Gels BIO-RAD Cat #4561093 Amicon Ultra 2 mL Centrifugal Filters Merck Cat # UFC200324 Superdex 200 10/300 GL GE Healthcare Life Sciences Cat # 17517501 Biacore T100 GE Healthcare Life Sciences RRID:SCR_008424 BD Accuri C6 flow cytometer BD Biosciences N/A Strep-Tactin XT protein purification system IBA GmbH Cat # 2-4090-002 Odyssey CLx Imaging System LI-COR Biosciences RRID:SCR_014579 Bio-Layer Interferometry instrument Octet K2 Pall ForteBio LLC N/A ..

    other:

    Article Title: Innate detection of Salmonella replication triggers caspase-8-dependent apoptosis via TLR-driven TNF signaling and NLRC4-mediated sensing of the SPI-2 Type III secretion system
    Article Snippet: ECL Western Blotting Substrate (Pierce/Thermo Scientific) was used as the HRP substrate for detection.

    CRISPR:

    Article Title: Tim4 Recognizes Carbon Nanotubes and Mediates Frustrated Phagocytosis Leading to Granuloma Formation
    Article Snippet: 1Graduate School of Information Sciences, Tohoku University, Sendai, Japan 2Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan 3Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Sendai, Japan 4Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, Japan 5Laboratory of Immunology and Microbiology, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Japan 6Department of Cancer Biology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan 7JST, PRESTO, Kawaguchi, Japan 8Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan 9Laboratory of Bioresponse Regulation, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan 10Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan 11Laboratory of Biochemistry and Immunology, Immunology Frontier Research Center, Osaka University, Suita, Japan 12Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan 13Lead Contact Email: mnakayam@fc.ritsumei.ac.jp; kengo@ecei.tohoku.ac.jp

    Knock-In:

    Article Title: Tim4 Recognizes Carbon Nanotubes and Mediates Frustrated Phagocytosis Leading to Granuloma Formation
    Article Snippet: 1Graduate School of Information Sciences, Tohoku University, Sendai, Japan 2Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan 3Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Sendai, Japan 4Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, Japan 5Laboratory of Immunology and Microbiology, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Japan 6Department of Cancer Biology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan 7JST, PRESTO, Kawaguchi, Japan 8Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan 9Laboratory of Bioresponse Regulation, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan 10Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan 11Laboratory of Biochemistry and Immunology, Immunology Frontier Research Center, Osaka University, Suita, Japan 12Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan 13Lead Contact Email: mnakayam@fc.ritsumei.ac.jp; kengo@ecei.tohoku.ac.jp

    Software:

    Article Title: Tim4 Recognizes Carbon Nanotubes and Mediates Frustrated Phagocytosis Leading to Granuloma Formation
    Article Snippet: 1Graduate School of Information Sciences, Tohoku University, Sendai, Japan 2Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan 3Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Sendai, Japan 4Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, Japan 5Laboratory of Immunology and Microbiology, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Japan 6Department of Cancer Biology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan 7JST, PRESTO, Kawaguchi, Japan 8Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan 9Laboratory of Bioresponse Regulation, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan 10Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan 11Laboratory of Biochemistry and Immunology, Immunology Frontier Research Center, Osaka University, Suita, Japan 12Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan 13Lead Contact Email: mnakayam@fc.ritsumei.ac.jp; kengo@ecei.tohoku.ac.jp

    Article Title: Discovery of Small Molecules that Activate RNA Methylation through Cooperative Binding to the METTL3-14-WTAP Complex Active Site.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Polyclonal rabbit-anti-m6A antibody Synaptic systems Cat#202 003; RRID:AB_2279214 Goat-anti-rabbit-IRDye 800CW LI-COR Biosciences Cat#926-32211; RRID:AB_621843 Goat-anti-mouse-IRDye 680LT LI-COR Biosciences RRID:AB_10706161 Monoclonal mouse-anti-FLAG M2 Sigma-Aldrich Cat#F1804; RRID:AB_262044 Polyclonal rabbit-anti-METTL3 Sigma-Aldrich Cat# HPA001299; RRID:AB_1079419 Polyclonal rabbit-anti-METTL14 Sigma-Aldrich Cat#HPA038002; RRID:AB_10672401 Monoclonal mouse-anti-WTAP Sigma-Aldrich Cat#SAB1402479, RRID:AB_10639633 Chemicals, Peptides, and Recombinant Proteins Methyl piperidine-3-carboxylate hydrochloride ArkPharm, Inc. AK-86479 Tert-butyl 6-methylpiperidine-3-carboxylate Life Chemicals Inc. F2163-0155 Methyl 6-methylpiperidine-3-carboxylate ArkPharm, Inc. AK103663 Methyl piperazine-2-carboxylate ChemDiv, Inc. FF20-0374 Reagent 007 for chemical transfection Icosagen, Ltd. Cat#R007-P010 EX-CELL 420 Serum-Free Medium Merck Cat#14420C InSolution Sinefungin Millipore Cat#567051 Propidium Iodide Invitrogen Cat#P3566 TRIzol Invitrogen Cat#15596018 Meclofenamate (sodium salt) Cayman chemicals Cat#70550 Nuclease P1 FUJIFILM Wako Chemicals Europe Cat#145-08221 Alkaline phosphatase Roche Cat#10713023001 Lipofectamine 2000 Ivitrogen Cat#11668019 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 3X FLAG Peptide Sigma-Aldrich Cat#F4799 RNaseOUT Recombinant Ribonuclease Inhibitor Ivitrogen Cat#10777019 Tritiated S-(5-adenosyl)-L–methionine Perkin Elmer Cat#NET155V250UC S-(5-Adenosyl)-L-methionine chloride dihydrochloride Sigma-Aldrich Cat#A7007 S-(5-Adenosyl)-L-homocysteine Sigma-Aldrich Cat#A9384 N6-methyladenosine (m6A) Selleckchem Cat#S3190 Precision Plus Protein Dual Color Standard BIO-RAD Cat#161-0374 Odyssey Blocking Buffer (PBS) LI-COR Biosciences Cat#927-40000 Adenosine Sigma-Aldrich Cat#A9251 Ammonium formate Fluka Cat#55674 Methanol (MeOH) HiPerSolv N/A Critical Commercial Assays PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems Series S Sensor Chip CM5 Series S Sensor Chip CM5 Series S Sensor Chip CM5 Amine Coupling Kit GE Healthcare Bio-Sciences Cat#BR-1000-50 Experimental Models: Cell Lines HEK293 ATCC CRL-1573 Sf9 Cell Line from Spodoptera frugiperda ThermoFisher Cat # 11496015 (Continued on next page) e1 Cell Reports 26, 3762–3771.e1–e5, March 26, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides 50-uacacucgaucuggacuaaagcugcuc-biotin-30 Li et al., 2016, Integrated DNA Technologies N/A Recombinant DNA Plasmid: 1xmut1 (METTL3/UniProt: Q86U44/ W373A) This paper N/A Plasmid: 1xmut2 (METTL3/UniProt: Q86U44/ D395A) This paper N/A Plasmid: 2xmut (METTL3 /UniProt: Q86U44/ W373A; K513A) This paper N/A pFastBac1 ThermoFisher N/A pcDNA3/Flag-METTL3 Liu et al., 2014, Addgene RRID:Addgene_53739 pcDNA3/Flag-METTL14 Liu et al., 2014, Addgene RRID:Addgene_53740 Software and Algorithms Schrödinger Suite 2015-3 Schrödinger LLC RRID:SCR_014879 Maestro 10.7 Schrödinger LLC RRID:SCR_016748 Desmond 3.6.0.0 Schrödinger LLC RRID:SCR_014575 AutoDock Tools 1.5.6 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 AutoDock 4.2 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 GraphPad prism 7 GraphPad Software / RRID:SCR_015807 ImageJ Schindelin et al., 2012 RRID:SCR_003070 BD Accuri C6 Plus BD Biosciences RRID:SCR_014422 Biacore T100 Control Software v. 2.0.3 GE Healthcare N/A Biacore T100 Evaluation Software v. 2.0.3 GE Healthcare N/A Other Millex Syringe Filter, Durapore (PVDF) Millipore Cat #SLGVR04NL FlashPlate Plus, Streptavidin Perkin Elmer Cat #SMP103A001PK 4–20% Mini-PROTEAN TGX Precast Protein Gels BIO-RAD Cat #4561093 Amicon Ultra 2 mL Centrifugal Filters Merck Cat # UFC200324 Superdex 200 10/300 GL GE Healthcare Life Sciences Cat # 17517501 Biacore T100 GE Healthcare Life Sciences RRID:SCR_008424 BD Accuri C6 flow cytometer BD Biosciences N/A Strep-Tactin XT protein purification system IBA GmbH Cat # 2-4090-002 Odyssey CLx Imaging System LI-COR Biosciences RRID:SCR_014579 Bio-Layer Interferometry instrument Octet K2 Pall ForteBio LLC N/A ..

    Recombinant:

    Article Title: Discovery of Small Molecules that Activate RNA Methylation through Cooperative Binding to the METTL3-14-WTAP Complex Active Site.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Polyclonal rabbit-anti-m6A antibody Synaptic systems Cat#202 003; RRID:AB_2279214 Goat-anti-rabbit-IRDye 800CW LI-COR Biosciences Cat#926-32211; RRID:AB_621843 Goat-anti-mouse-IRDye 680LT LI-COR Biosciences RRID:AB_10706161 Monoclonal mouse-anti-FLAG M2 Sigma-Aldrich Cat#F1804; RRID:AB_262044 Polyclonal rabbit-anti-METTL3 Sigma-Aldrich Cat# HPA001299; RRID:AB_1079419 Polyclonal rabbit-anti-METTL14 Sigma-Aldrich Cat#HPA038002; RRID:AB_10672401 Monoclonal mouse-anti-WTAP Sigma-Aldrich Cat#SAB1402479, RRID:AB_10639633 Chemicals, Peptides, and Recombinant Proteins Methyl piperidine-3-carboxylate hydrochloride ArkPharm, Inc. AK-86479 Tert-butyl 6-methylpiperidine-3-carboxylate Life Chemicals Inc. F2163-0155 Methyl 6-methylpiperidine-3-carboxylate ArkPharm, Inc. AK103663 Methyl piperazine-2-carboxylate ChemDiv, Inc. FF20-0374 Reagent 007 for chemical transfection Icosagen, Ltd. Cat#R007-P010 EX-CELL 420 Serum-Free Medium Merck Cat#14420C InSolution Sinefungin Millipore Cat#567051 Propidium Iodide Invitrogen Cat#P3566 TRIzol Invitrogen Cat#15596018 Meclofenamate (sodium salt) Cayman chemicals Cat#70550 Nuclease P1 FUJIFILM Wako Chemicals Europe Cat#145-08221 Alkaline phosphatase Roche Cat#10713023001 Lipofectamine 2000 Ivitrogen Cat#11668019 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 3X FLAG Peptide Sigma-Aldrich Cat#F4799 RNaseOUT Recombinant Ribonuclease Inhibitor Ivitrogen Cat#10777019 Tritiated S-(5-adenosyl)-L–methionine Perkin Elmer Cat#NET155V250UC S-(5-Adenosyl)-L-methionine chloride dihydrochloride Sigma-Aldrich Cat#A7007 S-(5-Adenosyl)-L-homocysteine Sigma-Aldrich Cat#A9384 N6-methyladenosine (m6A) Selleckchem Cat#S3190 Precision Plus Protein Dual Color Standard BIO-RAD Cat#161-0374 Odyssey Blocking Buffer (PBS) LI-COR Biosciences Cat#927-40000 Adenosine Sigma-Aldrich Cat#A9251 Ammonium formate Fluka Cat#55674 Methanol (MeOH) HiPerSolv N/A Critical Commercial Assays PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems Series S Sensor Chip CM5 Series S Sensor Chip CM5 Series S Sensor Chip CM5 Amine Coupling Kit GE Healthcare Bio-Sciences Cat#BR-1000-50 Experimental Models: Cell Lines HEK293 ATCC CRL-1573 Sf9 Cell Line from Spodoptera frugiperda ThermoFisher Cat # 11496015 (Continued on next page) e1 Cell Reports 26, 3762–3771.e1–e5, March 26, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides 50-uacacucgaucuggacuaaagcugcuc-biotin-30 Li et al., 2016, Integrated DNA Technologies N/A Recombinant DNA Plasmid: 1xmut1 (METTL3/UniProt: Q86U44/ W373A) This paper N/A Plasmid: 1xmut2 (METTL3/UniProt: Q86U44/ D395A) This paper N/A Plasmid: 2xmut (METTL3 /UniProt: Q86U44/ W373A; K513A) This paper N/A pFastBac1 ThermoFisher N/A pcDNA3/Flag-METTL3 Liu et al., 2014, Addgene RRID:Addgene_53739 pcDNA3/Flag-METTL14 Liu et al., 2014, Addgene RRID:Addgene_53740 Software and Algorithms Schrödinger Suite 2015-3 Schrödinger LLC RRID:SCR_014879 Maestro 10.7 Schrödinger LLC RRID:SCR_016748 Desmond 3.6.0.0 Schrödinger LLC RRID:SCR_014575 AutoDock Tools 1.5.6 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 AutoDock 4.2 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 GraphPad prism 7 GraphPad Software / RRID:SCR_015807 ImageJ Schindelin et al., 2012 RRID:SCR_003070 BD Accuri C6 Plus BD Biosciences RRID:SCR_014422 Biacore T100 Control Software v. 2.0.3 GE Healthcare N/A Biacore T100 Evaluation Software v. 2.0.3 GE Healthcare N/A Other Millex Syringe Filter, Durapore (PVDF) Millipore Cat #SLGVR04NL FlashPlate Plus, Streptavidin Perkin Elmer Cat #SMP103A001PK 4–20% Mini-PROTEAN TGX Precast Protein Gels BIO-RAD Cat #4561093 Amicon Ultra 2 mL Centrifugal Filters Merck Cat # UFC200324 Superdex 200 10/300 GL GE Healthcare Life Sciences Cat # 17517501 Biacore T100 GE Healthcare Life Sciences RRID:SCR_008424 BD Accuri C6 flow cytometer BD Biosciences N/A Strep-Tactin XT protein purification system IBA GmbH Cat # 2-4090-002 Odyssey CLx Imaging System LI-COR Biosciences RRID:SCR_014579 Bio-Layer Interferometry instrument Octet K2 Pall ForteBio LLC N/A ..

    Control:

    Article Title: Discovery of Small Molecules that Activate RNA Methylation through Cooperative Binding to the METTL3-14-WTAP Complex Active Site.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Polyclonal rabbit-anti-m6A antibody Synaptic systems Cat#202 003; RRID:AB_2279214 Goat-anti-rabbit-IRDye 800CW LI-COR Biosciences Cat#926-32211; RRID:AB_621843 Goat-anti-mouse-IRDye 680LT LI-COR Biosciences RRID:AB_10706161 Monoclonal mouse-anti-FLAG M2 Sigma-Aldrich Cat#F1804; RRID:AB_262044 Polyclonal rabbit-anti-METTL3 Sigma-Aldrich Cat# HPA001299; RRID:AB_1079419 Polyclonal rabbit-anti-METTL14 Sigma-Aldrich Cat#HPA038002; RRID:AB_10672401 Monoclonal mouse-anti-WTAP Sigma-Aldrich Cat#SAB1402479, RRID:AB_10639633 Chemicals, Peptides, and Recombinant Proteins Methyl piperidine-3-carboxylate hydrochloride ArkPharm, Inc. AK-86479 Tert-butyl 6-methylpiperidine-3-carboxylate Life Chemicals Inc. F2163-0155 Methyl 6-methylpiperidine-3-carboxylate ArkPharm, Inc. AK103663 Methyl piperazine-2-carboxylate ChemDiv, Inc. FF20-0374 Reagent 007 for chemical transfection Icosagen, Ltd. Cat#R007-P010 EX-CELL 420 Serum-Free Medium Merck Cat#14420C InSolution Sinefungin Millipore Cat#567051 Propidium Iodide Invitrogen Cat#P3566 TRIzol Invitrogen Cat#15596018 Meclofenamate (sodium salt) Cayman chemicals Cat#70550 Nuclease P1 FUJIFILM Wako Chemicals Europe Cat#145-08221 Alkaline phosphatase Roche Cat#10713023001 Lipofectamine 2000 Ivitrogen Cat#11668019 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 3X FLAG Peptide Sigma-Aldrich Cat#F4799 RNaseOUT Recombinant Ribonuclease Inhibitor Ivitrogen Cat#10777019 Tritiated S-(5-adenosyl)-L–methionine Perkin Elmer Cat#NET155V250UC S-(5-Adenosyl)-L-methionine chloride dihydrochloride Sigma-Aldrich Cat#A7007 S-(5-Adenosyl)-L-homocysteine Sigma-Aldrich Cat#A9384 N6-methyladenosine (m6A) Selleckchem Cat#S3190 Precision Plus Protein Dual Color Standard BIO-RAD Cat#161-0374 Odyssey Blocking Buffer (PBS) LI-COR Biosciences Cat#927-40000 Adenosine Sigma-Aldrich Cat#A9251 Ammonium formate Fluka Cat#55674 Methanol (MeOH) HiPerSolv N/A Critical Commercial Assays PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems Series S Sensor Chip CM5 Series S Sensor Chip CM5 Series S Sensor Chip CM5 Amine Coupling Kit GE Healthcare Bio-Sciences Cat#BR-1000-50 Experimental Models: Cell Lines HEK293 ATCC CRL-1573 Sf9 Cell Line from Spodoptera frugiperda ThermoFisher Cat # 11496015 (Continued on next page) e1 Cell Reports 26, 3762–3771.e1–e5, March 26, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides 50-uacacucgaucuggacuaaagcugcuc-biotin-30 Li et al., 2016, Integrated DNA Technologies N/A Recombinant DNA Plasmid: 1xmut1 (METTL3/UniProt: Q86U44/ W373A) This paper N/A Plasmid: 1xmut2 (METTL3/UniProt: Q86U44/ D395A) This paper N/A Plasmid: 2xmut (METTL3 /UniProt: Q86U44/ W373A; K513A) This paper N/A pFastBac1 ThermoFisher N/A pcDNA3/Flag-METTL3 Liu et al., 2014, Addgene RRID:Addgene_53739 pcDNA3/Flag-METTL14 Liu et al., 2014, Addgene RRID:Addgene_53740 Software and Algorithms Schrödinger Suite 2015-3 Schrödinger LLC RRID:SCR_014879 Maestro 10.7 Schrödinger LLC RRID:SCR_016748 Desmond 3.6.0.0 Schrödinger LLC RRID:SCR_014575 AutoDock Tools 1.5.6 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 AutoDock 4.2 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 GraphPad prism 7 GraphPad Software / RRID:SCR_015807 ImageJ Schindelin et al., 2012 RRID:SCR_003070 BD Accuri C6 Plus BD Biosciences RRID:SCR_014422 Biacore T100 Control Software v. 2.0.3 GE Healthcare N/A Biacore T100 Evaluation Software v. 2.0.3 GE Healthcare N/A Other Millex Syringe Filter, Durapore (PVDF) Millipore Cat #SLGVR04NL FlashPlate Plus, Streptavidin Perkin Elmer Cat #SMP103A001PK 4–20% Mini-PROTEAN TGX Precast Protein Gels BIO-RAD Cat #4561093 Amicon Ultra 2 mL Centrifugal Filters Merck Cat # UFC200324 Superdex 200 10/300 GL GE Healthcare Life Sciences Cat # 17517501 Biacore T100 GE Healthcare Life Sciences RRID:SCR_008424 BD Accuri C6 flow cytometer BD Biosciences N/A Strep-Tactin XT protein purification system IBA GmbH Cat # 2-4090-002 Odyssey CLx Imaging System LI-COR Biosciences RRID:SCR_014579 Bio-Layer Interferometry instrument Octet K2 Pall ForteBio LLC N/A ..

    Flow Cytometry:

    Article Title: Discovery of Small Molecules that Activate RNA Methylation through Cooperative Binding to the METTL3-14-WTAP Complex Active Site.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Polyclonal rabbit-anti-m6A antibody Synaptic systems Cat#202 003; RRID:AB_2279214 Goat-anti-rabbit-IRDye 800CW LI-COR Biosciences Cat#926-32211; RRID:AB_621843 Goat-anti-mouse-IRDye 680LT LI-COR Biosciences RRID:AB_10706161 Monoclonal mouse-anti-FLAG M2 Sigma-Aldrich Cat#F1804; RRID:AB_262044 Polyclonal rabbit-anti-METTL3 Sigma-Aldrich Cat# HPA001299; RRID:AB_1079419 Polyclonal rabbit-anti-METTL14 Sigma-Aldrich Cat#HPA038002; RRID:AB_10672401 Monoclonal mouse-anti-WTAP Sigma-Aldrich Cat#SAB1402479, RRID:AB_10639633 Chemicals, Peptides, and Recombinant Proteins Methyl piperidine-3-carboxylate hydrochloride ArkPharm, Inc. AK-86479 Tert-butyl 6-methylpiperidine-3-carboxylate Life Chemicals Inc. F2163-0155 Methyl 6-methylpiperidine-3-carboxylate ArkPharm, Inc. AK103663 Methyl piperazine-2-carboxylate ChemDiv, Inc. FF20-0374 Reagent 007 for chemical transfection Icosagen, Ltd. Cat#R007-P010 EX-CELL 420 Serum-Free Medium Merck Cat#14420C InSolution Sinefungin Millipore Cat#567051 Propidium Iodide Invitrogen Cat#P3566 TRIzol Invitrogen Cat#15596018 Meclofenamate (sodium salt) Cayman chemicals Cat#70550 Nuclease P1 FUJIFILM Wako Chemicals Europe Cat#145-08221 Alkaline phosphatase Roche Cat#10713023001 Lipofectamine 2000 Ivitrogen Cat#11668019 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 3X FLAG Peptide Sigma-Aldrich Cat#F4799 RNaseOUT Recombinant Ribonuclease Inhibitor Ivitrogen Cat#10777019 Tritiated S-(5-adenosyl)-L–methionine Perkin Elmer Cat#NET155V250UC S-(5-Adenosyl)-L-methionine chloride dihydrochloride Sigma-Aldrich Cat#A7007 S-(5-Adenosyl)-L-homocysteine Sigma-Aldrich Cat#A9384 N6-methyladenosine (m6A) Selleckchem Cat#S3190 Precision Plus Protein Dual Color Standard BIO-RAD Cat#161-0374 Odyssey Blocking Buffer (PBS) LI-COR Biosciences Cat#927-40000 Adenosine Sigma-Aldrich Cat#A9251 Ammonium formate Fluka Cat#55674 Methanol (MeOH) HiPerSolv N/A Critical Commercial Assays PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems Series S Sensor Chip CM5 Series S Sensor Chip CM5 Series S Sensor Chip CM5 Amine Coupling Kit GE Healthcare Bio-Sciences Cat#BR-1000-50 Experimental Models: Cell Lines HEK293 ATCC CRL-1573 Sf9 Cell Line from Spodoptera frugiperda ThermoFisher Cat # 11496015 (Continued on next page) e1 Cell Reports 26, 3762–3771.e1–e5, March 26, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides 50-uacacucgaucuggacuaaagcugcuc-biotin-30 Li et al., 2016, Integrated DNA Technologies N/A Recombinant DNA Plasmid: 1xmut1 (METTL3/UniProt: Q86U44/ W373A) This paper N/A Plasmid: 1xmut2 (METTL3/UniProt: Q86U44/ D395A) This paper N/A Plasmid: 2xmut (METTL3 /UniProt: Q86U44/ W373A; K513A) This paper N/A pFastBac1 ThermoFisher N/A pcDNA3/Flag-METTL3 Liu et al., 2014, Addgene RRID:Addgene_53739 pcDNA3/Flag-METTL14 Liu et al., 2014, Addgene RRID:Addgene_53740 Software and Algorithms Schrödinger Suite 2015-3 Schrödinger LLC RRID:SCR_014879 Maestro 10.7 Schrödinger LLC RRID:SCR_016748 Desmond 3.6.0.0 Schrödinger LLC RRID:SCR_014575 AutoDock Tools 1.5.6 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 AutoDock 4.2 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 GraphPad prism 7 GraphPad Software / RRID:SCR_015807 ImageJ Schindelin et al., 2012 RRID:SCR_003070 BD Accuri C6 Plus BD Biosciences RRID:SCR_014422 Biacore T100 Control Software v. 2.0.3 GE Healthcare N/A Biacore T100 Evaluation Software v. 2.0.3 GE Healthcare N/A Other Millex Syringe Filter, Durapore (PVDF) Millipore Cat #SLGVR04NL FlashPlate Plus, Streptavidin Perkin Elmer Cat #SMP103A001PK 4–20% Mini-PROTEAN TGX Precast Protein Gels BIO-RAD Cat #4561093 Amicon Ultra 2 mL Centrifugal Filters Merck Cat # UFC200324 Superdex 200 10/300 GL GE Healthcare Life Sciences Cat # 17517501 Biacore T100 GE Healthcare Life Sciences RRID:SCR_008424 BD Accuri C6 flow cytometer BD Biosciences N/A Strep-Tactin XT protein purification system IBA GmbH Cat # 2-4090-002 Odyssey CLx Imaging System LI-COR Biosciences RRID:SCR_014579 Bio-Layer Interferometry instrument Octet K2 Pall ForteBio LLC N/A ..

    Protein Purification:

    Article Title: Discovery of Small Molecules that Activate RNA Methylation through Cooperative Binding to the METTL3-14-WTAP Complex Active Site.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Polyclonal rabbit-anti-m6A antibody Synaptic systems Cat#202 003; RRID:AB_2279214 Goat-anti-rabbit-IRDye 800CW LI-COR Biosciences Cat#926-32211; RRID:AB_621843 Goat-anti-mouse-IRDye 680LT LI-COR Biosciences RRID:AB_10706161 Monoclonal mouse-anti-FLAG M2 Sigma-Aldrich Cat#F1804; RRID:AB_262044 Polyclonal rabbit-anti-METTL3 Sigma-Aldrich Cat# HPA001299; RRID:AB_1079419 Polyclonal rabbit-anti-METTL14 Sigma-Aldrich Cat#HPA038002; RRID:AB_10672401 Monoclonal mouse-anti-WTAP Sigma-Aldrich Cat#SAB1402479, RRID:AB_10639633 Chemicals, Peptides, and Recombinant Proteins Methyl piperidine-3-carboxylate hydrochloride ArkPharm, Inc. AK-86479 Tert-butyl 6-methylpiperidine-3-carboxylate Life Chemicals Inc. F2163-0155 Methyl 6-methylpiperidine-3-carboxylate ArkPharm, Inc. AK103663 Methyl piperazine-2-carboxylate ChemDiv, Inc. FF20-0374 Reagent 007 for chemical transfection Icosagen, Ltd. Cat#R007-P010 EX-CELL 420 Serum-Free Medium Merck Cat#14420C InSolution Sinefungin Millipore Cat#567051 Propidium Iodide Invitrogen Cat#P3566 TRIzol Invitrogen Cat#15596018 Meclofenamate (sodium salt) Cayman chemicals Cat#70550 Nuclease P1 FUJIFILM Wako Chemicals Europe Cat#145-08221 Alkaline phosphatase Roche Cat#10713023001 Lipofectamine 2000 Ivitrogen Cat#11668019 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 3X FLAG Peptide Sigma-Aldrich Cat#F4799 RNaseOUT Recombinant Ribonuclease Inhibitor Ivitrogen Cat#10777019 Tritiated S-(5-adenosyl)-L–methionine Perkin Elmer Cat#NET155V250UC S-(5-Adenosyl)-L-methionine chloride dihydrochloride Sigma-Aldrich Cat#A7007 S-(5-Adenosyl)-L-homocysteine Sigma-Aldrich Cat#A9384 N6-methyladenosine (m6A) Selleckchem Cat#S3190 Precision Plus Protein Dual Color Standard BIO-RAD Cat#161-0374 Odyssey Blocking Buffer (PBS) LI-COR Biosciences Cat#927-40000 Adenosine Sigma-Aldrich Cat#A9251 Ammonium formate Fluka Cat#55674 Methanol (MeOH) HiPerSolv N/A Critical Commercial Assays PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems Series S Sensor Chip CM5 Series S Sensor Chip CM5 Series S Sensor Chip CM5 Amine Coupling Kit GE Healthcare Bio-Sciences Cat#BR-1000-50 Experimental Models: Cell Lines HEK293 ATCC CRL-1573 Sf9 Cell Line from Spodoptera frugiperda ThermoFisher Cat # 11496015 (Continued on next page) e1 Cell Reports 26, 3762–3771.e1–e5, March 26, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides 50-uacacucgaucuggacuaaagcugcuc-biotin-30 Li et al., 2016, Integrated DNA Technologies N/A Recombinant DNA Plasmid: 1xmut1 (METTL3/UniProt: Q86U44/ W373A) This paper N/A Plasmid: 1xmut2 (METTL3/UniProt: Q86U44/ D395A) This paper N/A Plasmid: 2xmut (METTL3 /UniProt: Q86U44/ W373A; K513A) This paper N/A pFastBac1 ThermoFisher N/A pcDNA3/Flag-METTL3 Liu et al., 2014, Addgene RRID:Addgene_53739 pcDNA3/Flag-METTL14 Liu et al., 2014, Addgene RRID:Addgene_53740 Software and Algorithms Schrödinger Suite 2015-3 Schrödinger LLC RRID:SCR_014879 Maestro 10.7 Schrödinger LLC RRID:SCR_016748 Desmond 3.6.0.0 Schrödinger LLC RRID:SCR_014575 AutoDock Tools 1.5.6 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 AutoDock 4.2 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 GraphPad prism 7 GraphPad Software / RRID:SCR_015807 ImageJ Schindelin et al., 2012 RRID:SCR_003070 BD Accuri C6 Plus BD Biosciences RRID:SCR_014422 Biacore T100 Control Software v. 2.0.3 GE Healthcare N/A Biacore T100 Evaluation Software v. 2.0.3 GE Healthcare N/A Other Millex Syringe Filter, Durapore (PVDF) Millipore Cat #SLGVR04NL FlashPlate Plus, Streptavidin Perkin Elmer Cat #SMP103A001PK 4–20% Mini-PROTEAN TGX Precast Protein Gels BIO-RAD Cat #4561093 Amicon Ultra 2 mL Centrifugal Filters Merck Cat # UFC200324 Superdex 200 10/300 GL GE Healthcare Life Sciences Cat # 17517501 Biacore T100 GE Healthcare Life Sciences RRID:SCR_008424 BD Accuri C6 flow cytometer BD Biosciences N/A Strep-Tactin XT protein purification system IBA GmbH Cat # 2-4090-002 Odyssey CLx Imaging System LI-COR Biosciences RRID:SCR_014579 Bio-Layer Interferometry instrument Octet K2 Pall ForteBio LLC N/A ..

    Imaging:

    Article Title: Discovery of Small Molecules that Activate RNA Methylation through Cooperative Binding to the METTL3-14-WTAP Complex Active Site.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Polyclonal rabbit-anti-m6A antibody Synaptic systems Cat#202 003; RRID:AB_2279214 Goat-anti-rabbit-IRDye 800CW LI-COR Biosciences Cat#926-32211; RRID:AB_621843 Goat-anti-mouse-IRDye 680LT LI-COR Biosciences RRID:AB_10706161 Monoclonal mouse-anti-FLAG M2 Sigma-Aldrich Cat#F1804; RRID:AB_262044 Polyclonal rabbit-anti-METTL3 Sigma-Aldrich Cat# HPA001299; RRID:AB_1079419 Polyclonal rabbit-anti-METTL14 Sigma-Aldrich Cat#HPA038002; RRID:AB_10672401 Monoclonal mouse-anti-WTAP Sigma-Aldrich Cat#SAB1402479, RRID:AB_10639633 Chemicals, Peptides, and Recombinant Proteins Methyl piperidine-3-carboxylate hydrochloride ArkPharm, Inc. AK-86479 Tert-butyl 6-methylpiperidine-3-carboxylate Life Chemicals Inc. F2163-0155 Methyl 6-methylpiperidine-3-carboxylate ArkPharm, Inc. AK103663 Methyl piperazine-2-carboxylate ChemDiv, Inc. FF20-0374 Reagent 007 for chemical transfection Icosagen, Ltd. Cat#R007-P010 EX-CELL 420 Serum-Free Medium Merck Cat#14420C InSolution Sinefungin Millipore Cat#567051 Propidium Iodide Invitrogen Cat#P3566 TRIzol Invitrogen Cat#15596018 Meclofenamate (sodium salt) Cayman chemicals Cat#70550 Nuclease P1 FUJIFILM Wako Chemicals Europe Cat#145-08221 Alkaline phosphatase Roche Cat#10713023001 Lipofectamine 2000 Ivitrogen Cat#11668019 ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220 3X FLAG Peptide Sigma-Aldrich Cat#F4799 RNaseOUT Recombinant Ribonuclease Inhibitor Ivitrogen Cat#10777019 Tritiated S-(5-adenosyl)-L–methionine Perkin Elmer Cat#NET155V250UC S-(5-Adenosyl)-L-methionine chloride dihydrochloride Sigma-Aldrich Cat#A7007 S-(5-Adenosyl)-L-homocysteine Sigma-Aldrich Cat#A9384 N6-methyladenosine (m6A) Selleckchem Cat#S3190 Precision Plus Protein Dual Color Standard BIO-RAD Cat#161-0374 Odyssey Blocking Buffer (PBS) LI-COR Biosciences Cat#927-40000 Adenosine Sigma-Aldrich Cat#A9251 Ammonium formate Fluka Cat#55674 Methanol (MeOH) HiPerSolv N/A Critical Commercial Assays PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems PolyATtract mRNA Isolation Systems Series S Sensor Chip CM5 Series S Sensor Chip CM5 Series S Sensor Chip CM5 Amine Coupling Kit GE Healthcare Bio-Sciences Cat#BR-1000-50 Experimental Models: Cell Lines HEK293 ATCC CRL-1573 Sf9 Cell Line from Spodoptera frugiperda ThermoFisher Cat # 11496015 (Continued on next page) e1 Cell Reports 26, 3762–3771.e1–e5, March 26, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides 50-uacacucgaucuggacuaaagcugcuc-biotin-30 Li et al., 2016, Integrated DNA Technologies N/A Recombinant DNA Plasmid: 1xmut1 (METTL3/UniProt: Q86U44/ W373A) This paper N/A Plasmid: 1xmut2 (METTL3/UniProt: Q86U44/ D395A) This paper N/A Plasmid: 2xmut (METTL3 /UniProt: Q86U44/ W373A; K513A) This paper N/A pFastBac1 ThermoFisher N/A pcDNA3/Flag-METTL3 Liu et al., 2014, Addgene RRID:Addgene_53739 pcDNA3/Flag-METTL14 Liu et al., 2014, Addgene RRID:Addgene_53740 Software and Algorithms Schrödinger Suite 2015-3 Schrödinger LLC RRID:SCR_014879 Maestro 10.7 Schrödinger LLC RRID:SCR_016748 Desmond 3.6.0.0 Schrödinger LLC RRID:SCR_014575 AutoDock Tools 1.5.6 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 AutoDock 4.2 The Scripps Research Institute, Molecular Graphics laboratory RRID:SCR_012746 GraphPad prism 7 GraphPad Software / RRID:SCR_015807 ImageJ Schindelin et al., 2012 RRID:SCR_003070 BD Accuri C6 Plus BD Biosciences RRID:SCR_014422 Biacore T100 Control Software v. 2.0.3 GE Healthcare N/A Biacore T100 Evaluation Software v. 2.0.3 GE Healthcare N/A Other Millex Syringe Filter, Durapore (PVDF) Millipore Cat #SLGVR04NL FlashPlate Plus, Streptavidin Perkin Elmer Cat #SMP103A001PK 4–20% Mini-PROTEAN TGX Precast Protein Gels BIO-RAD Cat #4561093 Amicon Ultra 2 mL Centrifugal Filters Merck Cat # UFC200324 Superdex 200 10/300 GL GE Healthcare Life Sciences Cat # 17517501 Biacore T100 GE Healthcare Life Sciences RRID:SCR_008424 BD Accuri C6 flow cytometer BD Biosciences N/A Strep-Tactin XT protein purification system IBA GmbH Cat # 2-4090-002 Odyssey CLx Imaging System LI-COR Biosciences RRID:SCR_014579 Bio-Layer Interferometry instrument Octet K2 Pall ForteBio LLC N/A ..



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