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fibroblast growth factor, basic, human, recombinant (rhfgf, basic  (Promega)

 
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    Promega fibroblast growth factor, basic, human, recombinant (rhfgf, basic

    Fibroblast Growth Factor, Basic, Human, Recombinant (Rhfgf, Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhfgf+basic/recombinant+human+fibroblast+growth+factor/pmc11077435-38-0-9
    Average 90 stars, based on 1 article reviews
    fibroblast growth factor, basic, human, recombinant (rhfgf, basic - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "FACS-isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle"

    Article Title: FACS-isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle

    Journal: Journal of visualized experiments : JoVE

    doi: 10.3791/63983


    Figure Legend Snippet:

    Techniques Used: Sterility, Cell Culture, Recombinant

    Related Articles

    Recombinant:

    Article Title: Compromised nonsense-mediated RNA decay results in truncated RNA-binding protein production upon DUX4 expression.
    Article Snippet: 293T cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% EqualFETAL (Atlas Biologicals). .. Myoblasts were maintained in Ham’s F-10 Nutrient Mix (Thermo Fisher Scientific) supplemented with 20% Fetal Bovine Serum (Thermo Fisher Scientific), 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 mM dexamethasone (Sigma-Aldrich). ..

    Article Title: Temporal variation in p38-mediated regulation of DUX4 in facioscapulohumeral muscular dystrophy.
    Article Snippet: .. Myoblasts were grown in Ham’s F-10 Nutrient Mix (Gibco, Waltham, MA) supplemented with 20% USDA– approved source FBS (Corning, Corning, NY), 100 U/100 mg penicillin/streptomycin (Gibco), 10 ng/ml recombinant human fibroblast growth factor (Promega Corporation, Madison, WI), and 1 μM dexamethasone (Sigma-Aldrich, St. Louis, MO) and maintained at 37 °C in a humidified incubator with 5% CO2. ..

    Article Title: A Targeted Approach for Evaluating DUX4-Regulated Proteins as Potential Serum Biomarkers for Facioscapulohumeral Muscular Dystrophy Using Immunoassay Proteomics
    Article Snippet: .. Myoblasts were maintained in Ham’s F10 Nutrient Mix (Gibco) supplemented with 20% Fetal Bovine Serum (Gibco), 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 M dexamethasone (Sigma-Aldrich). ..

    Article Title: Compromised nonsense-mediated RNA decay results in truncated RNA-binding protein production upon DUX4 expression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-DUX4 (clone E5-5) Abcam Cat#ab124699; RRID:AB_10973363 Mouse monoclonal anti-eIF2a (clone D-3) Santa Cruz Biotechnology Cat#sc-133132; RRID:AB_1562699 Rabbit monoclonal anti-phospho-eIF2a (clone E90) Abcam Cat#ab32157; RRID:AB_732117 Rabbit polyclonal anti-Histone H3 Abcam Cat#ab1791; RRID:AB_302613 Mouse monoclonal anti-SRSF3 (clone 7B4 (7B4A12)) Thermo Fisher Scientific Cat#33–4200; RRID:AB_2533119 Rabbit polyclonal anti-SRSF3-TR This paper N/A Rabbit monoclonal anti-RENT1/hUPF1 (clone EPR4681) Abcam Cat#ab109363; RRID:AB_10861979 Chemicals, peptides, and recombinant proteins Insulin-Transferrin-Selenium Thermo Fisher Scientific Cat#41400045 Protein G Sepharose Thermo Fisher Scientific Cat#101241 Recombinant human basic fibroblast growth factor Promega CatG5071, discontinued Blasticidin S HCl Thermo Fisher Scientific Cat#R21001 Cycloheximide Sigma-Aldrich Cat#239765 Doxycycline hyclate Sigma-Aldrich Cat#D9891 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668-030 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778-150 MG132 Sigma-Aldrich Cat#474790 Puromycin dihydrochloride VWR Cat#97064-280 TRIzol Reagent Thermo Fisher Scientific Cat#15596018 Critical commercial assays BCA Protein Assay Kit Pierce Cat#23225 Caspase-Glo 3/7 Assay System Promega Cat#G8091 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2051 NEXTflex Rapid Directional qRNA-Seq Kit Bioo Scientific Cat# NOVA-5130-02D NEXTflex Small RNA-Seq Kit v3 Bioo Scientific Cat# NOVA-5132-06 Ribo-Zero rRNA Removal Kit Illumina Cat# MRZH11124, discontinued SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat#18080051 Deposited data RNA-seq and Ribo-seq data This paper GEO: GSE178761 Experimental models: Cell lines Human: 293T ATCC CRL-3216; RRID:CVCL_0063 Human: MB135 myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iDUX4 myoblasts Laboratory of Stephen Tapscott30 N/A Human: MB135-iDUX4/ZSCAN4-mCherry myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iFLAG-SRSF3-FL myoblasts This paper N/A Human: MB135-iFLAG-SRSF3-TR myoblasts This paper N/A Human: MB200 myoblasts Laboratory of Stephen Tapscott N/A (Continued on next page) 12 Cell Reports 42, 112642, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR, see Table S4 This paper N/A Antisense oligonucleotide: SRSF3_TMO-11: G*A*T*G*G*t*g*a*c*t*c*t*g*c*g*A*C*G*A*g (*, thiophosphoramidate or thiophosphate internucleotide linkage; capital letter, morpholino nucleoside; lowercase letter, 20deoxynucleoside) This paper N/A Recombinant DNA Plasmid: pCW57.1-FLAG-SRSF3_ Full.Length_Codon.Optimized-Blast This paper Addgene Plasmid #171951 Plasmid: pCW57.1-FLAG-SRSF3_ Truncated_Codon.Optimized-Blast This paper Addgene Plasmid #171952 Plasmid: pCW57-MCS1-P2A-MCS2 (Blast) Laboratory of Adam Karpf53 Addgene Plasmid #80921; RRID:Addgene_80921 Plasmid: pMD2.G Laboratory of Didier Trono Addgene Plasmid #12259; RRID:Addgene_12259 Plasmid: psPAX2 Laboratory of Didier Trono Addgene Plasmid #12260; RRID:Addgene_12260s Plasmid: pTwist-FLAG-SRSF3_Full.Length_ Codon.Optimized This paper Addgene Plasmid #172345 Plasmid: pTwist-FLAG-SRSF3_Truncated_ Codon.Optimized This paper Addgene Plasmid #172346 Software and algorithms Code used for RNA-seq and Ribo-seq figure generation This paper https://github.com/sjaganna/2021-campbell_ dyle_calviello_et_al; https://doi.org/10.5281/ zenodo.7951674 GraphPad Prism GraphPad Prism https://graphpad.com; RRID:SCR_002798 ImageJ ImageJ https://imagej.nih.gov; RRID:SCR:_003070

    Article Title: Temporal variation in p38-mediated regulation of DUX4 in facioscapulohumeral muscular dystrophy
    Article Snippet: .. Myoblasts were grown in Ham’s F-10 Nutrient Mix (Gibco, Waltham, MA) supplemented with 20% USDA–approved source FBS (Corning, Corning, NY), 100 U/100 mg penicillin/streptomycin (Gibco), 10 ng/ml recombinant human fibroblast growth factor (Promega Corporation, Madison, WI), and 1 μM dexamethasone (Sigma-Aldrich, St. Louis, MO) and maintained at 37 °C in a humidified incubator with 5% CO 2 . ..

    Article Title: FACS-isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
    Article Snippet: Falcon ® Round-Bottom Tubes, Polystyrene, with 35um Cell Strainer Cap Corning ® , VWR , 21008-948 , . .. Fibroblast Growth Factor, Basic, Human, Recombinant (rhFGF, Basic) , Promega , G5071 , . .. FlowJo 10.8.1 , , , .

    Article Title: A Targeted Approach for Evaluating DUX4-Regulated Proteins as Potential Serum Biomarkers for Facioscapulohumeral Muscular Dystrophy Using Immunoassay Proteomics
    Article Snippet: .. Myoblasts were maintained in Ham’s F-10 Nutrient Mix (Gibco) supplemented with 20% Fetal Bovine Serum (Gibco), 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μ M dexamethasone (Sigma-Aldrich). ..

    Transfection:

    Article Title: Compromised nonsense-mediated RNA decay results in truncated RNA-binding protein production upon DUX4 expression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-DUX4 (clone E5-5) Abcam Cat#ab124699; RRID:AB_10973363 Mouse monoclonal anti-eIF2a (clone D-3) Santa Cruz Biotechnology Cat#sc-133132; RRID:AB_1562699 Rabbit monoclonal anti-phospho-eIF2a (clone E90) Abcam Cat#ab32157; RRID:AB_732117 Rabbit polyclonal anti-Histone H3 Abcam Cat#ab1791; RRID:AB_302613 Mouse monoclonal anti-SRSF3 (clone 7B4 (7B4A12)) Thermo Fisher Scientific Cat#33–4200; RRID:AB_2533119 Rabbit polyclonal anti-SRSF3-TR This paper N/A Rabbit monoclonal anti-RENT1/hUPF1 (clone EPR4681) Abcam Cat#ab109363; RRID:AB_10861979 Chemicals, peptides, and recombinant proteins Insulin-Transferrin-Selenium Thermo Fisher Scientific Cat#41400045 Protein G Sepharose Thermo Fisher Scientific Cat#101241 Recombinant human basic fibroblast growth factor Promega CatG5071, discontinued Blasticidin S HCl Thermo Fisher Scientific Cat#R21001 Cycloheximide Sigma-Aldrich Cat#239765 Doxycycline hyclate Sigma-Aldrich Cat#D9891 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668-030 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778-150 MG132 Sigma-Aldrich Cat#474790 Puromycin dihydrochloride VWR Cat#97064-280 TRIzol Reagent Thermo Fisher Scientific Cat#15596018 Critical commercial assays BCA Protein Assay Kit Pierce Cat#23225 Caspase-Glo 3/7 Assay System Promega Cat#G8091 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2051 NEXTflex Rapid Directional qRNA-Seq Kit Bioo Scientific Cat# NOVA-5130-02D NEXTflex Small RNA-Seq Kit v3 Bioo Scientific Cat# NOVA-5132-06 Ribo-Zero rRNA Removal Kit Illumina Cat# MRZH11124, discontinued SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat#18080051 Deposited data RNA-seq and Ribo-seq data This paper GEO: GSE178761 Experimental models: Cell lines Human: 293T ATCC CRL-3216; RRID:CVCL_0063 Human: MB135 myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iDUX4 myoblasts Laboratory of Stephen Tapscott30 N/A Human: MB135-iDUX4/ZSCAN4-mCherry myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iFLAG-SRSF3-FL myoblasts This paper N/A Human: MB135-iFLAG-SRSF3-TR myoblasts This paper N/A Human: MB200 myoblasts Laboratory of Stephen Tapscott N/A (Continued on next page) 12 Cell Reports 42, 112642, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR, see Table S4 This paper N/A Antisense oligonucleotide: SRSF3_TMO-11: G*A*T*G*G*t*g*a*c*t*c*t*g*c*g*A*C*G*A*g (*, thiophosphoramidate or thiophosphate internucleotide linkage; capital letter, morpholino nucleoside; lowercase letter, 20deoxynucleoside) This paper N/A Recombinant DNA Plasmid: pCW57.1-FLAG-SRSF3_ Full.Length_Codon.Optimized-Blast This paper Addgene Plasmid #171951 Plasmid: pCW57.1-FLAG-SRSF3_ Truncated_Codon.Optimized-Blast This paper Addgene Plasmid #171952 Plasmid: pCW57-MCS1-P2A-MCS2 (Blast) Laboratory of Adam Karpf53 Addgene Plasmid #80921; RRID:Addgene_80921 Plasmid: pMD2.G Laboratory of Didier Trono Addgene Plasmid #12259; RRID:Addgene_12259 Plasmid: psPAX2 Laboratory of Didier Trono Addgene Plasmid #12260; RRID:Addgene_12260s Plasmid: pTwist-FLAG-SRSF3_Full.Length_ Codon.Optimized This paper Addgene Plasmid #172345 Plasmid: pTwist-FLAG-SRSF3_Truncated_ Codon.Optimized This paper Addgene Plasmid #172346 Software and algorithms Code used for RNA-seq and Ribo-seq figure generation This paper https://github.com/sjaganna/2021-campbell_ dyle_calviello_et_al; https://doi.org/10.5281/ zenodo.7951674 GraphPad Prism GraphPad Prism https://graphpad.com; RRID:SCR_002798 ImageJ ImageJ https://imagej.nih.gov; RRID:SCR:_003070

    Bicinchoninic Acid Protein Assay:

    Article Title: Compromised nonsense-mediated RNA decay results in truncated RNA-binding protein production upon DUX4 expression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-DUX4 (clone E5-5) Abcam Cat#ab124699; RRID:AB_10973363 Mouse monoclonal anti-eIF2a (clone D-3) Santa Cruz Biotechnology Cat#sc-133132; RRID:AB_1562699 Rabbit monoclonal anti-phospho-eIF2a (clone E90) Abcam Cat#ab32157; RRID:AB_732117 Rabbit polyclonal anti-Histone H3 Abcam Cat#ab1791; RRID:AB_302613 Mouse monoclonal anti-SRSF3 (clone 7B4 (7B4A12)) Thermo Fisher Scientific Cat#33–4200; RRID:AB_2533119 Rabbit polyclonal anti-SRSF3-TR This paper N/A Rabbit monoclonal anti-RENT1/hUPF1 (clone EPR4681) Abcam Cat#ab109363; RRID:AB_10861979 Chemicals, peptides, and recombinant proteins Insulin-Transferrin-Selenium Thermo Fisher Scientific Cat#41400045 Protein G Sepharose Thermo Fisher Scientific Cat#101241 Recombinant human basic fibroblast growth factor Promega CatG5071, discontinued Blasticidin S HCl Thermo Fisher Scientific Cat#R21001 Cycloheximide Sigma-Aldrich Cat#239765 Doxycycline hyclate Sigma-Aldrich Cat#D9891 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668-030 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778-150 MG132 Sigma-Aldrich Cat#474790 Puromycin dihydrochloride VWR Cat#97064-280 TRIzol Reagent Thermo Fisher Scientific Cat#15596018 Critical commercial assays BCA Protein Assay Kit Pierce Cat#23225 Caspase-Glo 3/7 Assay System Promega Cat#G8091 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2051 NEXTflex Rapid Directional qRNA-Seq Kit Bioo Scientific Cat# NOVA-5130-02D NEXTflex Small RNA-Seq Kit v3 Bioo Scientific Cat# NOVA-5132-06 Ribo-Zero rRNA Removal Kit Illumina Cat# MRZH11124, discontinued SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat#18080051 Deposited data RNA-seq and Ribo-seq data This paper GEO: GSE178761 Experimental models: Cell lines Human: 293T ATCC CRL-3216; RRID:CVCL_0063 Human: MB135 myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iDUX4 myoblasts Laboratory of Stephen Tapscott30 N/A Human: MB135-iDUX4/ZSCAN4-mCherry myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iFLAG-SRSF3-FL myoblasts This paper N/A Human: MB135-iFLAG-SRSF3-TR myoblasts This paper N/A Human: MB200 myoblasts Laboratory of Stephen Tapscott N/A (Continued on next page) 12 Cell Reports 42, 112642, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR, see Table S4 This paper N/A Antisense oligonucleotide: SRSF3_TMO-11: G*A*T*G*G*t*g*a*c*t*c*t*g*c*g*A*C*G*A*g (*, thiophosphoramidate or thiophosphate internucleotide linkage; capital letter, morpholino nucleoside; lowercase letter, 20deoxynucleoside) This paper N/A Recombinant DNA Plasmid: pCW57.1-FLAG-SRSF3_ Full.Length_Codon.Optimized-Blast This paper Addgene Plasmid #171951 Plasmid: pCW57.1-FLAG-SRSF3_ Truncated_Codon.Optimized-Blast This paper Addgene Plasmid #171952 Plasmid: pCW57-MCS1-P2A-MCS2 (Blast) Laboratory of Adam Karpf53 Addgene Plasmid #80921; RRID:Addgene_80921 Plasmid: pMD2.G Laboratory of Didier Trono Addgene Plasmid #12259; RRID:Addgene_12259 Plasmid: psPAX2 Laboratory of Didier Trono Addgene Plasmid #12260; RRID:Addgene_12260s Plasmid: pTwist-FLAG-SRSF3_Full.Length_ Codon.Optimized This paper Addgene Plasmid #172345 Plasmid: pTwist-FLAG-SRSF3_Truncated_ Codon.Optimized This paper Addgene Plasmid #172346 Software and algorithms Code used for RNA-seq and Ribo-seq figure generation This paper https://github.com/sjaganna/2021-campbell_ dyle_calviello_et_al; https://doi.org/10.5281/ zenodo.7951674 GraphPad Prism GraphPad Prism https://graphpad.com; RRID:SCR_002798 ImageJ ImageJ https://imagej.nih.gov; RRID:SCR:_003070

    Caspase-Glo Assay:

    Article Title: Compromised nonsense-mediated RNA decay results in truncated RNA-binding protein production upon DUX4 expression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-DUX4 (clone E5-5) Abcam Cat#ab124699; RRID:AB_10973363 Mouse monoclonal anti-eIF2a (clone D-3) Santa Cruz Biotechnology Cat#sc-133132; RRID:AB_1562699 Rabbit monoclonal anti-phospho-eIF2a (clone E90) Abcam Cat#ab32157; RRID:AB_732117 Rabbit polyclonal anti-Histone H3 Abcam Cat#ab1791; RRID:AB_302613 Mouse monoclonal anti-SRSF3 (clone 7B4 (7B4A12)) Thermo Fisher Scientific Cat#33–4200; RRID:AB_2533119 Rabbit polyclonal anti-SRSF3-TR This paper N/A Rabbit monoclonal anti-RENT1/hUPF1 (clone EPR4681) Abcam Cat#ab109363; RRID:AB_10861979 Chemicals, peptides, and recombinant proteins Insulin-Transferrin-Selenium Thermo Fisher Scientific Cat#41400045 Protein G Sepharose Thermo Fisher Scientific Cat#101241 Recombinant human basic fibroblast growth factor Promega CatG5071, discontinued Blasticidin S HCl Thermo Fisher Scientific Cat#R21001 Cycloheximide Sigma-Aldrich Cat#239765 Doxycycline hyclate Sigma-Aldrich Cat#D9891 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668-030 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778-150 MG132 Sigma-Aldrich Cat#474790 Puromycin dihydrochloride VWR Cat#97064-280 TRIzol Reagent Thermo Fisher Scientific Cat#15596018 Critical commercial assays BCA Protein Assay Kit Pierce Cat#23225 Caspase-Glo 3/7 Assay System Promega Cat#G8091 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2051 NEXTflex Rapid Directional qRNA-Seq Kit Bioo Scientific Cat# NOVA-5130-02D NEXTflex Small RNA-Seq Kit v3 Bioo Scientific Cat# NOVA-5132-06 Ribo-Zero rRNA Removal Kit Illumina Cat# MRZH11124, discontinued SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat#18080051 Deposited data RNA-seq and Ribo-seq data This paper GEO: GSE178761 Experimental models: Cell lines Human: 293T ATCC CRL-3216; RRID:CVCL_0063 Human: MB135 myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iDUX4 myoblasts Laboratory of Stephen Tapscott30 N/A Human: MB135-iDUX4/ZSCAN4-mCherry myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iFLAG-SRSF3-FL myoblasts This paper N/A Human: MB135-iFLAG-SRSF3-TR myoblasts This paper N/A Human: MB200 myoblasts Laboratory of Stephen Tapscott N/A (Continued on next page) 12 Cell Reports 42, 112642, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR, see Table S4 This paper N/A Antisense oligonucleotide: SRSF3_TMO-11: G*A*T*G*G*t*g*a*c*t*c*t*g*c*g*A*C*G*A*g (*, thiophosphoramidate or thiophosphate internucleotide linkage; capital letter, morpholino nucleoside; lowercase letter, 20deoxynucleoside) This paper N/A Recombinant DNA Plasmid: pCW57.1-FLAG-SRSF3_ Full.Length_Codon.Optimized-Blast This paper Addgene Plasmid #171951 Plasmid: pCW57.1-FLAG-SRSF3_ Truncated_Codon.Optimized-Blast This paper Addgene Plasmid #171952 Plasmid: pCW57-MCS1-P2A-MCS2 (Blast) Laboratory of Adam Karpf53 Addgene Plasmid #80921; RRID:Addgene_80921 Plasmid: pMD2.G Laboratory of Didier Trono Addgene Plasmid #12259; RRID:Addgene_12259 Plasmid: psPAX2 Laboratory of Didier Trono Addgene Plasmid #12260; RRID:Addgene_12260s Plasmid: pTwist-FLAG-SRSF3_Full.Length_ Codon.Optimized This paper Addgene Plasmid #172345 Plasmid: pTwist-FLAG-SRSF3_Truncated_ Codon.Optimized This paper Addgene Plasmid #172346 Software and algorithms Code used for RNA-seq and Ribo-seq figure generation This paper https://github.com/sjaganna/2021-campbell_ dyle_calviello_et_al; https://doi.org/10.5281/ zenodo.7951674 GraphPad Prism GraphPad Prism https://graphpad.com; RRID:SCR_002798 ImageJ ImageJ https://imagej.nih.gov; RRID:SCR:_003070

    RNA sequencing:

    Article Title: Compromised nonsense-mediated RNA decay results in truncated RNA-binding protein production upon DUX4 expression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-DUX4 (clone E5-5) Abcam Cat#ab124699; RRID:AB_10973363 Mouse monoclonal anti-eIF2a (clone D-3) Santa Cruz Biotechnology Cat#sc-133132; RRID:AB_1562699 Rabbit monoclonal anti-phospho-eIF2a (clone E90) Abcam Cat#ab32157; RRID:AB_732117 Rabbit polyclonal anti-Histone H3 Abcam Cat#ab1791; RRID:AB_302613 Mouse monoclonal anti-SRSF3 (clone 7B4 (7B4A12)) Thermo Fisher Scientific Cat#33–4200; RRID:AB_2533119 Rabbit polyclonal anti-SRSF3-TR This paper N/A Rabbit monoclonal anti-RENT1/hUPF1 (clone EPR4681) Abcam Cat#ab109363; RRID:AB_10861979 Chemicals, peptides, and recombinant proteins Insulin-Transferrin-Selenium Thermo Fisher Scientific Cat#41400045 Protein G Sepharose Thermo Fisher Scientific Cat#101241 Recombinant human basic fibroblast growth factor Promega CatG5071, discontinued Blasticidin S HCl Thermo Fisher Scientific Cat#R21001 Cycloheximide Sigma-Aldrich Cat#239765 Doxycycline hyclate Sigma-Aldrich Cat#D9891 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668-030 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778-150 MG132 Sigma-Aldrich Cat#474790 Puromycin dihydrochloride VWR Cat#97064-280 TRIzol Reagent Thermo Fisher Scientific Cat#15596018 Critical commercial assays BCA Protein Assay Kit Pierce Cat#23225 Caspase-Glo 3/7 Assay System Promega Cat#G8091 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2051 NEXTflex Rapid Directional qRNA-Seq Kit Bioo Scientific Cat# NOVA-5130-02D NEXTflex Small RNA-Seq Kit v3 Bioo Scientific Cat# NOVA-5132-06 Ribo-Zero rRNA Removal Kit Illumina Cat# MRZH11124, discontinued SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat#18080051 Deposited data RNA-seq and Ribo-seq data This paper GEO: GSE178761 Experimental models: Cell lines Human: 293T ATCC CRL-3216; RRID:CVCL_0063 Human: MB135 myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iDUX4 myoblasts Laboratory of Stephen Tapscott30 N/A Human: MB135-iDUX4/ZSCAN4-mCherry myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iFLAG-SRSF3-FL myoblasts This paper N/A Human: MB135-iFLAG-SRSF3-TR myoblasts This paper N/A Human: MB200 myoblasts Laboratory of Stephen Tapscott N/A (Continued on next page) 12 Cell Reports 42, 112642, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR, see Table S4 This paper N/A Antisense oligonucleotide: SRSF3_TMO-11: G*A*T*G*G*t*g*a*c*t*c*t*g*c*g*A*C*G*A*g (*, thiophosphoramidate or thiophosphate internucleotide linkage; capital letter, morpholino nucleoside; lowercase letter, 20deoxynucleoside) This paper N/A Recombinant DNA Plasmid: pCW57.1-FLAG-SRSF3_ Full.Length_Codon.Optimized-Blast This paper Addgene Plasmid #171951 Plasmid: pCW57.1-FLAG-SRSF3_ Truncated_Codon.Optimized-Blast This paper Addgene Plasmid #171952 Plasmid: pCW57-MCS1-P2A-MCS2 (Blast) Laboratory of Adam Karpf53 Addgene Plasmid #80921; RRID:Addgene_80921 Plasmid: pMD2.G Laboratory of Didier Trono Addgene Plasmid #12259; RRID:Addgene_12259 Plasmid: psPAX2 Laboratory of Didier Trono Addgene Plasmid #12260; RRID:Addgene_12260s Plasmid: pTwist-FLAG-SRSF3_Full.Length_ Codon.Optimized This paper Addgene Plasmid #172345 Plasmid: pTwist-FLAG-SRSF3_Truncated_ Codon.Optimized This paper Addgene Plasmid #172346 Software and algorithms Code used for RNA-seq and Ribo-seq figure generation This paper https://github.com/sjaganna/2021-campbell_ dyle_calviello_et_al; https://doi.org/10.5281/ zenodo.7951674 GraphPad Prism GraphPad Prism https://graphpad.com; RRID:SCR_002798 ImageJ ImageJ https://imagej.nih.gov; RRID:SCR:_003070

    RNA Sequencing:

    Article Title: Compromised nonsense-mediated RNA decay results in truncated RNA-binding protein production upon DUX4 expression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-DUX4 (clone E5-5) Abcam Cat#ab124699; RRID:AB_10973363 Mouse monoclonal anti-eIF2a (clone D-3) Santa Cruz Biotechnology Cat#sc-133132; RRID:AB_1562699 Rabbit monoclonal anti-phospho-eIF2a (clone E90) Abcam Cat#ab32157; RRID:AB_732117 Rabbit polyclonal anti-Histone H3 Abcam Cat#ab1791; RRID:AB_302613 Mouse monoclonal anti-SRSF3 (clone 7B4 (7B4A12)) Thermo Fisher Scientific Cat#33–4200; RRID:AB_2533119 Rabbit polyclonal anti-SRSF3-TR This paper N/A Rabbit monoclonal anti-RENT1/hUPF1 (clone EPR4681) Abcam Cat#ab109363; RRID:AB_10861979 Chemicals, peptides, and recombinant proteins Insulin-Transferrin-Selenium Thermo Fisher Scientific Cat#41400045 Protein G Sepharose Thermo Fisher Scientific Cat#101241 Recombinant human basic fibroblast growth factor Promega CatG5071, discontinued Blasticidin S HCl Thermo Fisher Scientific Cat#R21001 Cycloheximide Sigma-Aldrich Cat#239765 Doxycycline hyclate Sigma-Aldrich Cat#D9891 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668-030 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778-150 MG132 Sigma-Aldrich Cat#474790 Puromycin dihydrochloride VWR Cat#97064-280 TRIzol Reagent Thermo Fisher Scientific Cat#15596018 Critical commercial assays BCA Protein Assay Kit Pierce Cat#23225 Caspase-Glo 3/7 Assay System Promega Cat#G8091 Direct-zol RNA Miniprep Kit Zymo Research Cat#R2051 NEXTflex Rapid Directional qRNA-Seq Kit Bioo Scientific Cat# NOVA-5130-02D NEXTflex Small RNA-Seq Kit v3 Bioo Scientific Cat# NOVA-5132-06 Ribo-Zero rRNA Removal Kit Illumina Cat# MRZH11124, discontinued SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat#18080051 Deposited data RNA-seq and Ribo-seq data This paper GEO: GSE178761 Experimental models: Cell lines Human: 293T ATCC CRL-3216; RRID:CVCL_0063 Human: MB135 myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iDUX4 myoblasts Laboratory of Stephen Tapscott30 N/A Human: MB135-iDUX4/ZSCAN4-mCherry myoblasts Laboratory of Stephen Tapscott N/A Human: MB135-iFLAG-SRSF3-FL myoblasts This paper N/A Human: MB135-iFLAG-SRSF3-TR myoblasts This paper N/A Human: MB200 myoblasts Laboratory of Stephen Tapscott N/A (Continued on next page) 12 Cell Reports 42, 112642, June 27, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR, see Table S4 This paper N/A Antisense oligonucleotide: SRSF3_TMO-11: G*A*T*G*G*t*g*a*c*t*c*t*g*c*g*A*C*G*A*g (*, thiophosphoramidate or thiophosphate internucleotide linkage; capital letter, morpholino nucleoside; lowercase letter, 20deoxynucleoside) This paper N/A Recombinant DNA Plasmid: pCW57.1-FLAG-SRSF3_ Full.Length_Codon.Optimized-Blast This paper Addgene Plasmid #171951 Plasmid: pCW57.1-FLAG-SRSF3_ Truncated_Codon.Optimized-Blast This paper Addgene Plasmid #171952 Plasmid: pCW57-MCS1-P2A-MCS2 (Blast) Laboratory of Adam Karpf53 Addgene Plasmid #80921; RRID:Addgene_80921 Plasmid: pMD2.G Laboratory of Didier Trono Addgene Plasmid #12259; RRID:Addgene_12259 Plasmid: psPAX2 Laboratory of Didier Trono Addgene Plasmid #12260; RRID:Addgene_12260s Plasmid: pTwist-FLAG-SRSF3_Full.Length_ Codon.Optimized This paper Addgene Plasmid #172345 Plasmid: pTwist-FLAG-SRSF3_Truncated_ Codon.Optimized This paper Addgene Plasmid #172346 Software and algorithms Code used for RNA-seq and Ribo-seq figure generation This paper https://github.com/sjaganna/2021-campbell_ dyle_calviello_et_al; https://doi.org/10.5281/ zenodo.7951674 GraphPad Prism GraphPad Prism https://graphpad.com; RRID:SCR_002798 ImageJ ImageJ https://imagej.nih.gov; RRID:SCR:_003070



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    Journal: Journal of visualized experiments : JoVE

    Article Title: FACS-isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle

    doi: 10.3791/63983

    Figure Lengend Snippet:

    Article Snippet: Fibroblast Growth Factor, Basic, Human, Recombinant (rhFGF, Basic) , Promega , G5071 , .

    Techniques: Sterility, Cell Culture, Recombinant