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reverse phase protein microarrays  (Aushon Biosystems)

 
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    Aushon Biosystems reverse phase protein microarrays
    Reverse Phase Protein Microarrays, supplied by Aushon Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse-phase+protein+microarray/protein+arrays+aushon/pmc03501264-374-0-11
    Average 90 stars, based on 1 article reviews
    reverse phase protein microarrays - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Kruppel-like factor 10 (KLF10) as a biomarker of endothelial progenitor cell dysfunction
    Article Snippet: .. ELISA The supernatants from cultured CMPs, GMPs, MEPs and HSCs were collected and subjected to for ELISA using the SearchLight multiplex protein arrays (Aushon Biosystems, Billerica, Mass., USA). .. Intracellular Staining of KLF10 in Human PACs Peripheral blood samples (20 mL) were obtained from healthy subjects and patients with peripheral artery disease, diabetes, and stent thrombosis in accordance with approval by Investigation Review Boards at Brigham and Women's Hospital (Boston, Mass., USA) and Rabin Medical Center (Petah Tikva, Israel).

    Cell Culture:

    Article Title: Kruppel-like factor 10 (KLF10) as a biomarker of endothelial progenitor cell dysfunction
    Article Snippet: .. ELISA The supernatants from cultured CMPs, GMPs, MEPs and HSCs were collected and subjected to for ELISA using the SearchLight multiplex protein arrays (Aushon Biosystems, Billerica, Mass., USA). .. Intracellular Staining of KLF10 in Human PACs Peripheral blood samples (20 mL) were obtained from healthy subjects and patients with peripheral artery disease, diabetes, and stent thrombosis in accordance with approval by Investigation Review Boards at Brigham and Women's Hospital (Boston, Mass., USA) and Rabin Medical Center (Petah Tikva, Israel).

    Multiplex Assay:

    Article Title: Kruppel-like factor 10 (KLF10) as a biomarker of endothelial progenitor cell dysfunction
    Article Snippet: .. ELISA The supernatants from cultured CMPs, GMPs, MEPs and HSCs were collected and subjected to for ELISA using the SearchLight multiplex protein arrays (Aushon Biosystems, Billerica, Mass., USA). .. Intracellular Staining of KLF10 in Human PACs Peripheral blood samples (20 mL) were obtained from healthy subjects and patients with peripheral artery disease, diabetes, and stent thrombosis in accordance with approval by Investigation Review Boards at Brigham and Women's Hospital (Boston, Mass., USA) and Rabin Medical Center (Petah Tikva, Israel).

    Article Title: A pilot study to assess the feasibility of a remotely monitored high-intensity interval training program prior to allogeneic hematopoietic stem cell transplantation
    Article Snippet: .. In order to develop the optimal arrays, we have the opportunity to leverage 3 multiplex protein array systems from Aushon Biosystems (Billerica, MA), ProteinSimple (San Jose, CA) and Meso Scale Diagnostic (MSD), LLC. (Rockville, MD). .. In order to develop the optimal arrays, we have the opportunity to leverage 3 multiplex protein array systems from Aushon Biosystems (Billerica, MA), ProteinSimple (San Jose, CA) and Meso Scale Diagnostic (MSD), LLC. (Rockville, MD).

    other:

    Article Title: Genetic ablation of epidermal EGFR reveals the dynamic origin of adverse effects of anti-EGFR therapy
    Article Snippet: Clodronate treatment Clodronate liposomes and control PBS liposomes were purchased from Encapsula NanoSciences.

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    Article Snippet: Clodronate liposomes and control PBS liposomes were purchased from Encapsula NanoSciences.

    Article Title: Methods and non-immunogenic compositions for treating inflammatory disorders
    Article Snippet: The solution is generated by adding 6 mL of PRP to a modified Plasmax device containing 1 gram of polyacrylamide beads (Biomet Biologics, Warsaw, Ind.).

    Article Title: Comprehensive molecular characterization of pheochromocytoma and paraganglioma
    Article Snippet: Reverse phase protein array , Aushon Biosystems 2470; CanoScan 9000F , 76 , open.

    Protein Array:

    Article Title: Human Bone Marrow Stromal Cell Confluence: Effects on Cell Characteristics and Methods of Assessment
    Article Snippet: Measurement of Glucose and Lactate, Growth Factors and Cytokines in Culture Supernatants The concentrations of glucose and lactate in the culture supernatant of BMSC were measured daily by using an i-STAT Analyzer (Abbott, Princeton, NJ). .. The concentrations of cytokine and growth factors in BMSC supernatants were measured with SearchLight Protein Array (Aushon Biosystems, Billerica, MA). .. The concentrations of cytokine and growth factors in BMSC supernatants were measured with SearchLight Protein Array (Aushon Biosystems, Billerica, MA).

    Article Title: A pilot study to assess the feasibility of a remotely monitored high-intensity interval training program prior to allogeneic hematopoietic stem cell transplantation
    Article Snippet: .. In order to develop the optimal arrays, we have the opportunity to leverage 3 multiplex protein array systems from Aushon Biosystems (Billerica, MA), ProteinSimple (San Jose, CA) and Meso Scale Diagnostic (MSD), LLC. (Rockville, MD). .. In order to develop the optimal arrays, we have the opportunity to leverage 3 multiplex protein array systems from Aushon Biosystems (Billerica, MA), ProteinSimple (San Jose, CA) and Meso Scale Diagnostic (MSD), LLC. (Rockville, MD).

    Article Title: Comprehensive molecular characterization of pheochromocytoma and paraganglioma
    Article Snippet: .. The integrated clinical and genomic datasets are available through the NCI’s Genomic Data Commons. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort. .. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort.

    Diagnostic Assay:

    Article Title: A pilot study to assess the feasibility of a remotely monitored high-intensity interval training program prior to allogeneic hematopoietic stem cell transplantation
    Article Snippet: .. In order to develop the optimal arrays, we have the opportunity to leverage 3 multiplex protein array systems from Aushon Biosystems (Billerica, MA), ProteinSimple (San Jose, CA) and Meso Scale Diagnostic (MSD), LLC. (Rockville, MD). .. In order to develop the optimal arrays, we have the opportunity to leverage 3 multiplex protein array systems from Aushon Biosystems (Billerica, MA), ProteinSimple (San Jose, CA) and Meso Scale Diagnostic (MSD), LLC. (Rockville, MD).

    DNA Sequencing:

    Article Title: Comprehensive molecular characterization of pheochromocytoma and paraganglioma
    Article Snippet: .. The integrated clinical and genomic datasets are available through the NCI’s Genomic Data Commons. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort. .. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort.

    Sequencing:

    Article Title: Comprehensive molecular characterization of pheochromocytoma and paraganglioma
    Article Snippet: .. The integrated clinical and genomic datasets are available through the NCI’s Genomic Data Commons. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort. .. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort.

    Expressing:

    Article Title: Comprehensive molecular characterization of pheochromocytoma and paraganglioma
    Article Snippet: .. The integrated clinical and genomic datasets are available through the NCI’s Genomic Data Commons. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort. .. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort.

    DNA Methylation Assay:

    Article Title: Comprehensive molecular characterization of pheochromocytoma and paraganglioma
    Article Snippet: .. The integrated clinical and genomic datasets are available through the NCI’s Genomic Data Commons. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort. .. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort.

    Periodic Counter-current Chromatography:

    Article Title: Comprehensive molecular characterization of pheochromocytoma and paraganglioma
    Article Snippet: .. The integrated clinical and genomic datasets are available through the NCI’s Genomic Data Commons. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort. .. table ft1 table-wrap mode="anchored" t5 caption a7 Data Type Platforms Cases (n) Data Access TCGA Core Sample Set (n = 173 total cases) Whole-exome DNA sequencing Illumina; SureSelect v2 173 controlled: BAM files open: somatic mutations DNA copy number Affymetrix SNP 6.0 173 controlled: CEL files open: copy number mRNA sequencing Illumina 173 controlled: BAM files open: expression miRNA sequencing Illumina 173 controlled: BAM files open: expression CpG DNA methylation Illuminia Infinium HM 450 173 open Reverse phase protein array Aushon Biosystems 2470; CanoScan 9000F 76 open Open in a separate window Summary of Data Types Germline and Somatic Mutations Because susceptibility gene mutations are prevalent in patients with PCC/PGLs, we first analyzed DNA exome sequencing of normal specimens to identify germline mutations in the cohort.



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    90
    Cell Signaling Technology Inc reverse phase protein microarray
    A discrete role for the alpha catalytic PI3K isoform in proneural (PN) GSCs. ( A ) Expression z-score data from TCGA (Extended Verhaak) for PIK3CA (left panel), PIK3CB (middle panel) and PIK3CD (right panel) were downloaded from the GBM-BioDP ( http://gbm-biodp.nci.nih.gov/ ) for CL (n = 105), MES (n = 124) and PN (n = 113) subtype GBM from the HT Human Genome U133 (HT_HG-U133A) array and analysed using GraphPad Prism 8. As recent evidence suggests the neural subtype represents excessive contamination with normal brain, it has been excluded from the analysis. Unpaired one-way ANOVA, * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ( B ) Survival analysis based on the impact of the multi-gene prognostic index for coexpression of PIK3CA and NES (upper panels), PIK3CB and NES (middle panels) or PIK3CD and NES (lower panels) for CL (n = 53, left panels), MES (n = 57, middle panels) and PN (n = 56, right panels) subtype. TCGA gene expression data (Verhaak Core) for PIK3CA and NES , PIK3CB and NES or PIK3CD and NES from the HT_HG-U133A array were used for multigene prognostic index. Figure was generated using the GBM-BioDP software. ( C ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against NF1, p110α and GAPDH. Membrane was stripped and reprobed with an antibody against p110β. Lysates from the same experiment were run in parallel and probed for p110 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\updelta $$\end{document} δ and GAPDH. ( D ) Reverse-phase protein <t>microarray</t> <t>(RPPA)</t> data from TCGA GBM samples. Boxplots of RPPA analytes for phospho-AKT(Ser 473 ) measured in CL (n = 28), MES (n = 29) and PN (n = 41) samples from the cohort of GBM tumours (Extended Verhaak) that was subjected to RPPA analysis within the TCGA consortium. RPPA data were downloaded from the GBM-BioDP and analysed using GraphPad Prism 8. Unpaired one-way ANOVA: * p ≤ 0.05. ( E ) Survival analysis based on RPPA analysis for phospho-AKT(Ser 473 ) using the Extended Verhaak cohort submitted to the TCGA consortium for CL (n = 28, left panel), MES (n = 29, middle panel) and PN (n = 41, right panel) subtypes. Figure was generated using the GBM-BioDP software. ( F ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against AKT. Membrane was stripped and re-probed for p-AKT(Thr 308 ), then stripped and reprobed for p-AKT(Ser 473 ), then stripped and re-probed for HSP90.
    Reverse Phase Protein Microarray, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reverse phase protein microarray workflow  (Federation of European Neuroscience Societies)
    90
    Federation of European Neuroscience Societies reverse phase protein microarray workflow
    A discrete role for the alpha catalytic PI3K isoform in proneural (PN) GSCs. ( A ) Expression z-score data from TCGA (Extended Verhaak) for PIK3CA (left panel), PIK3CB (middle panel) and PIK3CD (right panel) were downloaded from the GBM-BioDP ( http://gbm-biodp.nci.nih.gov/ ) for CL (n = 105), MES (n = 124) and PN (n = 113) subtype GBM from the HT Human Genome U133 (HT_HG-U133A) array and analysed using GraphPad Prism 8. As recent evidence suggests the neural subtype represents excessive contamination with normal brain, it has been excluded from the analysis. Unpaired one-way ANOVA, * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ( B ) Survival analysis based on the impact of the multi-gene prognostic index for coexpression of PIK3CA and NES (upper panels), PIK3CB and NES (middle panels) or PIK3CD and NES (lower panels) for CL (n = 53, left panels), MES (n = 57, middle panels) and PN (n = 56, right panels) subtype. TCGA gene expression data (Verhaak Core) for PIK3CA and NES , PIK3CB and NES or PIK3CD and NES from the HT_HG-U133A array were used for multigene prognostic index. Figure was generated using the GBM-BioDP software. ( C ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against NF1, p110α and GAPDH. Membrane was stripped and reprobed with an antibody against p110β. Lysates from the same experiment were run in parallel and probed for p110 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\updelta $$\end{document} δ and GAPDH. ( D ) Reverse-phase protein <t>microarray</t> <t>(RPPA)</t> data from TCGA GBM samples. Boxplots of RPPA analytes for phospho-AKT(Ser 473 ) measured in CL (n = 28), MES (n = 29) and PN (n = 41) samples from the cohort of GBM tumours (Extended Verhaak) that was subjected to RPPA analysis within the TCGA consortium. RPPA data were downloaded from the GBM-BioDP and analysed using GraphPad Prism 8. Unpaired one-way ANOVA: * p ≤ 0.05. ( E ) Survival analysis based on RPPA analysis for phospho-AKT(Ser 473 ) using the Extended Verhaak cohort submitted to the TCGA consortium for CL (n = 28, left panel), MES (n = 29, middle panel) and PN (n = 41, right panel) subtypes. Figure was generated using the GBM-BioDP software. ( F ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against AKT. Membrane was stripped and re-probed for p-AKT(Thr 308 ), then stripped and reprobed for p-AKT(Ser 473 ), then stripped and re-probed for HSP90.
    Reverse Phase Protein Microarray Workflow, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reverse phase protein microarray aushon 2470 arrayer  (Aushon Biosystems)
    90
    Aushon Biosystems reverse phase protein microarray aushon 2470 arrayer
    A discrete role for the alpha catalytic PI3K isoform in proneural (PN) GSCs. ( A ) Expression z-score data from TCGA (Extended Verhaak) for PIK3CA (left panel), PIK3CB (middle panel) and PIK3CD (right panel) were downloaded from the GBM-BioDP ( http://gbm-biodp.nci.nih.gov/ ) for CL (n = 105), MES (n = 124) and PN (n = 113) subtype GBM from the HT Human Genome U133 (HT_HG-U133A) array and analysed using GraphPad Prism 8. As recent evidence suggests the neural subtype represents excessive contamination with normal brain, it has been excluded from the analysis. Unpaired one-way ANOVA, * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ( B ) Survival analysis based on the impact of the multi-gene prognostic index for coexpression of PIK3CA and NES (upper panels), PIK3CB and NES (middle panels) or PIK3CD and NES (lower panels) for CL (n = 53, left panels), MES (n = 57, middle panels) and PN (n = 56, right panels) subtype. TCGA gene expression data (Verhaak Core) for PIK3CA and NES , PIK3CB and NES or PIK3CD and NES from the HT_HG-U133A array were used for multigene prognostic index. Figure was generated using the GBM-BioDP software. ( C ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against NF1, p110α and GAPDH. Membrane was stripped and reprobed with an antibody against p110β. Lysates from the same experiment were run in parallel and probed for p110 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\updelta $$\end{document} δ and GAPDH. ( D ) Reverse-phase protein <t>microarray</t> <t>(RPPA)</t> data from TCGA GBM samples. Boxplots of RPPA analytes for phospho-AKT(Ser 473 ) measured in CL (n = 28), MES (n = 29) and PN (n = 41) samples from the cohort of GBM tumours (Extended Verhaak) that was subjected to RPPA analysis within the TCGA consortium. RPPA data were downloaded from the GBM-BioDP and analysed using GraphPad Prism 8. Unpaired one-way ANOVA: * p ≤ 0.05. ( E ) Survival analysis based on RPPA analysis for phospho-AKT(Ser 473 ) using the Extended Verhaak cohort submitted to the TCGA consortium for CL (n = 28, left panel), MES (n = 29, middle panel) and PN (n = 41, right panel) subtypes. Figure was generated using the GBM-BioDP software. ( F ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against AKT. Membrane was stripped and re-probed for p-AKT(Thr 308 ), then stripped and reprobed for p-AKT(Ser 473 ), then stripped and re-probed for HSP90.
    Reverse Phase Protein Microarray Aushon 2470 Arrayer, supplied by Aushon Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reverse phase protein microarrays  (Aushon Biosystems)
    90
    Aushon Biosystems reverse phase protein microarrays
    A discrete role for the alpha catalytic PI3K isoform in proneural (PN) GSCs. ( A ) Expression z-score data from TCGA (Extended Verhaak) for PIK3CA (left panel), PIK3CB (middle panel) and PIK3CD (right panel) were downloaded from the GBM-BioDP ( http://gbm-biodp.nci.nih.gov/ ) for CL (n = 105), MES (n = 124) and PN (n = 113) subtype GBM from the HT Human Genome U133 (HT_HG-U133A) array and analysed using GraphPad Prism 8. As recent evidence suggests the neural subtype represents excessive contamination with normal brain, it has been excluded from the analysis. Unpaired one-way ANOVA, * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ( B ) Survival analysis based on the impact of the multi-gene prognostic index for coexpression of PIK3CA and NES (upper panels), PIK3CB and NES (middle panels) or PIK3CD and NES (lower panels) for CL (n = 53, left panels), MES (n = 57, middle panels) and PN (n = 56, right panels) subtype. TCGA gene expression data (Verhaak Core) for PIK3CA and NES , PIK3CB and NES or PIK3CD and NES from the HT_HG-U133A array were used for multigene prognostic index. Figure was generated using the GBM-BioDP software. ( C ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against NF1, p110α and GAPDH. Membrane was stripped and reprobed with an antibody against p110β. Lysates from the same experiment were run in parallel and probed for p110 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\updelta $$\end{document} δ and GAPDH. ( D ) Reverse-phase protein <t>microarray</t> <t>(RPPA)</t> data from TCGA GBM samples. Boxplots of RPPA analytes for phospho-AKT(Ser 473 ) measured in CL (n = 28), MES (n = 29) and PN (n = 41) samples from the cohort of GBM tumours (Extended Verhaak) that was subjected to RPPA analysis within the TCGA consortium. RPPA data were downloaded from the GBM-BioDP and analysed using GraphPad Prism 8. Unpaired one-way ANOVA: * p ≤ 0.05. ( E ) Survival analysis based on RPPA analysis for phospho-AKT(Ser 473 ) using the Extended Verhaak cohort submitted to the TCGA consortium for CL (n = 28, left panel), MES (n = 29, middle panel) and PN (n = 41, right panel) subtypes. Figure was generated using the GBM-BioDP software. ( F ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against AKT. Membrane was stripped and re-probed for p-AKT(Thr 308 ), then stripped and reprobed for p-AKT(Ser 473 ), then stripped and re-probed for HSP90.
    Reverse Phase Protein Microarrays, supplied by Aushon Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse-phase+protein+microarray/protein+arrays+aushon/pmc03501264-374-0-11
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    reverse phase protein microarray  (Aushon Biosystems)
    90
    Aushon Biosystems reverse phase protein microarray
    A discrete role for the alpha catalytic PI3K isoform in proneural (PN) GSCs. ( A ) Expression z-score data from TCGA (Extended Verhaak) for PIK3CA (left panel), PIK3CB (middle panel) and PIK3CD (right panel) were downloaded from the GBM-BioDP ( http://gbm-biodp.nci.nih.gov/ ) for CL (n = 105), MES (n = 124) and PN (n = 113) subtype GBM from the HT Human Genome U133 (HT_HG-U133A) array and analysed using GraphPad Prism 8. As recent evidence suggests the neural subtype represents excessive contamination with normal brain, it has been excluded from the analysis. Unpaired one-way ANOVA, * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ( B ) Survival analysis based on the impact of the multi-gene prognostic index for coexpression of PIK3CA and NES (upper panels), PIK3CB and NES (middle panels) or PIK3CD and NES (lower panels) for CL (n = 53, left panels), MES (n = 57, middle panels) and PN (n = 56, right panels) subtype. TCGA gene expression data (Verhaak Core) for PIK3CA and NES , PIK3CB and NES or PIK3CD and NES from the HT_HG-U133A array were used for multigene prognostic index. Figure was generated using the GBM-BioDP software. ( C ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against NF1, p110α and GAPDH. Membrane was stripped and reprobed with an antibody against p110β. Lysates from the same experiment were run in parallel and probed for p110 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\updelta $$\end{document} δ and GAPDH. ( D ) Reverse-phase protein <t>microarray</t> <t>(RPPA)</t> data from TCGA GBM samples. Boxplots of RPPA analytes for phospho-AKT(Ser 473 ) measured in CL (n = 28), MES (n = 29) and PN (n = 41) samples from the cohort of GBM tumours (Extended Verhaak) that was subjected to RPPA analysis within the TCGA consortium. RPPA data were downloaded from the GBM-BioDP and analysed using GraphPad Prism 8. Unpaired one-way ANOVA: * p ≤ 0.05. ( E ) Survival analysis based on RPPA analysis for phospho-AKT(Ser 473 ) using the Extended Verhaak cohort submitted to the TCGA consortium for CL (n = 28, left panel), MES (n = 29, middle panel) and PN (n = 41, right panel) subtypes. Figure was generated using the GBM-BioDP software. ( F ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against AKT. Membrane was stripped and re-probed for p-AKT(Thr 308 ), then stripped and reprobed for p-AKT(Ser 473 ), then stripped and re-probed for HSP90.
    Reverse Phase Protein Microarray, supplied by Aushon Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Summary of molecular findings in the Side Out 2 trial based on patients’ GMIs. Frequencies of gene expression, protein expression by immunohistochemistry and protein activation by RPPA (Panel A, Panel B, and Panel C, respectively) for 22 of the 25 patients enrolled in the Side Out 2 trial.

    Journal: Molecular Oncology

    Article Title: Multi‐omic molecular profiling guide’s efficacious treatment selection in refractory metastatic breast cancer: a prospective phase II clinical trial

    doi: 10.1002/1878-0261.13091

    Figure Lengend Snippet: Summary of molecular findings in the Side Out 2 trial based on patients’ GMIs. Frequencies of gene expression, protein expression by immunohistochemistry and protein activation by RPPA (Panel A, Panel B, and Panel C, respectively) for 22 of the 25 patients enrolled in the Side Out 2 trial.

    Article Snippet: Patient‐matched fresh frozen material was used by the Center for Applied Proteomics and Molecular Medicine at George Mason University for protein pathway activation mapping using laser capture microdissection (LCM) to isolate tumor epithelia followed by Reverse Phase Protein Microarray (RPPA) analysis as previously described [ ].

    Techniques: Gene Expression, Expressing, Immunohistochemistry, Activation Assay

    A discrete role for the alpha catalytic PI3K isoform in proneural (PN) GSCs. ( A ) Expression z-score data from TCGA (Extended Verhaak) for PIK3CA (left panel), PIK3CB (middle panel) and PIK3CD (right panel) were downloaded from the GBM-BioDP ( http://gbm-biodp.nci.nih.gov/ ) for CL (n = 105), MES (n = 124) and PN (n = 113) subtype GBM from the HT Human Genome U133 (HT_HG-U133A) array and analysed using GraphPad Prism 8. As recent evidence suggests the neural subtype represents excessive contamination with normal brain, it has been excluded from the analysis. Unpaired one-way ANOVA, * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ( B ) Survival analysis based on the impact of the multi-gene prognostic index for coexpression of PIK3CA and NES (upper panels), PIK3CB and NES (middle panels) or PIK3CD and NES (lower panels) for CL (n = 53, left panels), MES (n = 57, middle panels) and PN (n = 56, right panels) subtype. TCGA gene expression data (Verhaak Core) for PIK3CA and NES , PIK3CB and NES or PIK3CD and NES from the HT_HG-U133A array were used for multigene prognostic index. Figure was generated using the GBM-BioDP software. ( C ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against NF1, p110α and GAPDH. Membrane was stripped and reprobed with an antibody against p110β. Lysates from the same experiment were run in parallel and probed for p110 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\updelta $$\end{document} δ and GAPDH. ( D ) Reverse-phase protein microarray (RPPA) data from TCGA GBM samples. Boxplots of RPPA analytes for phospho-AKT(Ser 473 ) measured in CL (n = 28), MES (n = 29) and PN (n = 41) samples from the cohort of GBM tumours (Extended Verhaak) that was subjected to RPPA analysis within the TCGA consortium. RPPA data were downloaded from the GBM-BioDP and analysed using GraphPad Prism 8. Unpaired one-way ANOVA: * p ≤ 0.05. ( E ) Survival analysis based on RPPA analysis for phospho-AKT(Ser 473 ) using the Extended Verhaak cohort submitted to the TCGA consortium for CL (n = 28, left panel), MES (n = 29, middle panel) and PN (n = 41, right panel) subtypes. Figure was generated using the GBM-BioDP software. ( F ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against AKT. Membrane was stripped and re-probed for p-AKT(Thr 308 ), then stripped and reprobed for p-AKT(Ser 473 ), then stripped and re-probed for HSP90.

    Journal: Scientific Reports

    Article Title: Combined PI3Kα-mTOR Targeting of Glioma Stem Cells

    doi: 10.1038/s41598-020-78788-z

    Figure Lengend Snippet: A discrete role for the alpha catalytic PI3K isoform in proneural (PN) GSCs. ( A ) Expression z-score data from TCGA (Extended Verhaak) for PIK3CA (left panel), PIK3CB (middle panel) and PIK3CD (right panel) were downloaded from the GBM-BioDP ( http://gbm-biodp.nci.nih.gov/ ) for CL (n = 105), MES (n = 124) and PN (n = 113) subtype GBM from the HT Human Genome U133 (HT_HG-U133A) array and analysed using GraphPad Prism 8. As recent evidence suggests the neural subtype represents excessive contamination with normal brain, it has been excluded from the analysis. Unpaired one-way ANOVA, * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ( B ) Survival analysis based on the impact of the multi-gene prognostic index for coexpression of PIK3CA and NES (upper panels), PIK3CB and NES (middle panels) or PIK3CD and NES (lower panels) for CL (n = 53, left panels), MES (n = 57, middle panels) and PN (n = 56, right panels) subtype. TCGA gene expression data (Verhaak Core) for PIK3CA and NES , PIK3CB and NES or PIK3CD and NES from the HT_HG-U133A array were used for multigene prognostic index. Figure was generated using the GBM-BioDP software. ( C ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against NF1, p110α and GAPDH. Membrane was stripped and reprobed with an antibody against p110β. Lysates from the same experiment were run in parallel and probed for p110 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\updelta $$\end{document} δ and GAPDH. ( D ) Reverse-phase protein microarray (RPPA) data from TCGA GBM samples. Boxplots of RPPA analytes for phospho-AKT(Ser 473 ) measured in CL (n = 28), MES (n = 29) and PN (n = 41) samples from the cohort of GBM tumours (Extended Verhaak) that was subjected to RPPA analysis within the TCGA consortium. RPPA data were downloaded from the GBM-BioDP and analysed using GraphPad Prism 8. Unpaired one-way ANOVA: * p ≤ 0.05. ( E ) Survival analysis based on RPPA analysis for phospho-AKT(Ser 473 ) using the Extended Verhaak cohort submitted to the TCGA consortium for CL (n = 28, left panel), MES (n = 29, middle panel) and PN (n = 41, right panel) subtypes. Figure was generated using the GBM-BioDP software. ( F ) GSC lines JK16, 83Mes, 157PN and AC17PN were analysed by Western blot using antibodies against AKT. Membrane was stripped and re-probed for p-AKT(Thr 308 ), then stripped and reprobed for p-AKT(Ser 473 ), then stripped and re-probed for HSP90.

    Article Snippet: Similarly, protein Reverse Phase Protein Microarray (RPPA) data of the Extended Verhaak dataset for p-AKT (S473) were downloaded and analysed using GraphPad Prism 8.

    Techniques: Expressing, Gene Expression, Generated, Software, Western Blot, Membrane, Microarray