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rt reaction  (highQu Inc)


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    Structured Review

    highQu Inc rt reaction
    Rt Reaction, supplied by highQu Inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcriptase/HighScriber+Reverse+Transcriptase+Mix/pmc11317168-102-1-37
    Average 93 stars, based on 6 article reviews
    rt reaction - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model
    Article Snippet: RNA of samples was isolated using the NucleoSpin RNA isolation kit (Macherey–Nagel, #740955.250) according to the manufacturer’s instructions. .. 20 ng of RNA (as measured by a NanoDrop ND-100 spectrometer) were used for one-step SYBR Green-based reverse transcriptase PCR using the 1 Step RT PCR Green ROX L Kit (highQu) according to the manufacturer’s instruction. .. Specific fragments (all Quantitect Primer Assay, Qiagen) used, were KCNN4 (K Ca 3.1; QT00105672), TGFBR1 (QT00135828), MMP2 (QT00116116), MMP9 (QT00108815) and CD44 (QT00173404).

    Article Title: Logical regulation of endogenous gene expression using programmable, multi-input processing CRISPR guide RNAs
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse transcribed using the cDNA Synthesis Kit (Promega, Madison, WI, USA). .. The RT reaction was performed at 37°C for 15 min, followed by inactivation of reverse transcriptase at 85°C for 15 s using a thermal cycler. qPCR experiments were conducted using ORATM qPCR Green ROX L Mix (2×) (highQu, Kraichtal, Germany) with specific qPCR primers for target RNA or 16S rRNA. .. A Stratagene Mx3000P thermal cycler was used with specified conditions.

    Article Title: One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap
    Article Snippet: The supernatant was further diluted 2 μL in 198 μL extraction buffer General (10 X diluted to 1 X, Bioreba AG, Switzerland) and 2 μL of this dilution were used for the DiRT. .. The mastermix for the multiplex DiRT-PCRs were done using the same primers, probes, fluorophore-combinations as described in Prinz et al. (2022) using an optimized concentration protocol version with HighScriber reverse transcriptase and HighScriber Buffer (HighQu, Germany, for details, see Supp3). ..

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model.
    Article Snippet: RNA of samples was isolated using the NucleoSpin RNA isolation kit (Macherey–Nagel, #740955.250) according to the manufacturer’s instructions. .. 20 ng of RNA (as measured by a NanoDrop ND-100 spectrometer) were used for one-step SYBR Green-based reverse transcriptase PCR using the 1 Step RT PCR Green ROX L Kit (highQu) according to the manufacturer’s instruction. .. Specific fragments (all Quantitect Primer Assay, Qiagen) used, were KCNN4 (KCa3.1; QT00105672), TGFBR1 (QT00135828), MMP2 (QT00116116), MMP9 (QT00108815) and CD44 (QT00173404).

    Polymerase Chain Reaction:

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model
    Article Snippet: RNA of samples was isolated using the NucleoSpin RNA isolation kit (Macherey–Nagel, #740955.250) according to the manufacturer’s instructions. .. 20 ng of RNA (as measured by a NanoDrop ND-100 spectrometer) were used for one-step SYBR Green-based reverse transcriptase PCR using the 1 Step RT PCR Green ROX L Kit (highQu) according to the manufacturer’s instruction. .. Specific fragments (all Quantitect Primer Assay, Qiagen) used, were KCNN4 (K Ca 3.1; QT00105672), TGFBR1 (QT00135828), MMP2 (QT00116116), MMP9 (QT00108815) and CD44 (QT00173404).

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model.
    Article Snippet: RNA of samples was isolated using the NucleoSpin RNA isolation kit (Macherey–Nagel, #740955.250) according to the manufacturer’s instructions. .. 20 ng of RNA (as measured by a NanoDrop ND-100 spectrometer) were used for one-step SYBR Green-based reverse transcriptase PCR using the 1 Step RT PCR Green ROX L Kit (highQu) according to the manufacturer’s instruction. .. Specific fragments (all Quantitect Primer Assay, Qiagen) used, were KCNN4 (KCa3.1; QT00105672), TGFBR1 (QT00135828), MMP2 (QT00116116), MMP9 (QT00108815) and CD44 (QT00173404).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model
    Article Snippet: RNA of samples was isolated using the NucleoSpin RNA isolation kit (Macherey–Nagel, #740955.250) according to the manufacturer’s instructions. .. 20 ng of RNA (as measured by a NanoDrop ND-100 spectrometer) were used for one-step SYBR Green-based reverse transcriptase PCR using the 1 Step RT PCR Green ROX L Kit (highQu) according to the manufacturer’s instruction. .. Specific fragments (all Quantitect Primer Assay, Qiagen) used, were KCNN4 (K Ca 3.1; QT00105672), TGFBR1 (QT00135828), MMP2 (QT00116116), MMP9 (QT00108815) and CD44 (QT00173404).

    Article Title: Efficacy of combined tumor irradiation and K Ca 3.1-targeting with TRAM-34 in a syngeneic glioma mouse model.
    Article Snippet: RNA of samples was isolated using the NucleoSpin RNA isolation kit (Macherey–Nagel, #740955.250) according to the manufacturer’s instructions. .. 20 ng of RNA (as measured by a NanoDrop ND-100 spectrometer) were used for one-step SYBR Green-based reverse transcriptase PCR using the 1 Step RT PCR Green ROX L Kit (highQu) according to the manufacturer’s instruction. .. Specific fragments (all Quantitect Primer Assay, Qiagen) used, were KCNN4 (KCa3.1; QT00105672), TGFBR1 (QT00135828), MMP2 (QT00116116), MMP9 (QT00108815) and CD44 (QT00173404).

    Real-time Polymerase Chain Reaction:

    Article Title: Logical regulation of endogenous gene expression using programmable, multi-input processing CRISPR guide RNAs
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse transcribed using the cDNA Synthesis Kit (Promega, Madison, WI, USA). .. The RT reaction was performed at 37°C for 15 min, followed by inactivation of reverse transcriptase at 85°C for 15 s using a thermal cycler. qPCR experiments were conducted using ORATM qPCR Green ROX L Mix (2×) (highQu, Kraichtal, Germany) with specific qPCR primers for target RNA or 16S rRNA. .. A Stratagene Mx3000P thermal cycler was used with specified conditions.

    Multiplex Assay:

    Article Title: One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap
    Article Snippet: The supernatant was further diluted 2 μL in 198 μL extraction buffer General (10 X diluted to 1 X, Bioreba AG, Switzerland) and 2 μL of this dilution were used for the DiRT. .. The mastermix for the multiplex DiRT-PCRs were done using the same primers, probes, fluorophore-combinations as described in Prinz et al. (2022) using an optimized concentration protocol version with HighScriber reverse transcriptase and HighScriber Buffer (HighQu, Germany, for details, see Supp3). ..

    Concentration Assay:

    Article Title: One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap
    Article Snippet: The supernatant was further diluted 2 μL in 198 μL extraction buffer General (10 X diluted to 1 X, Bioreba AG, Switzerland) and 2 μL of this dilution were used for the DiRT. .. The mastermix for the multiplex DiRT-PCRs were done using the same primers, probes, fluorophore-combinations as described in Prinz et al. (2022) using an optimized concentration protocol version with HighScriber reverse transcriptase and HighScriber Buffer (HighQu, Germany, for details, see Supp3). ..



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