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Lucigen Corp nxgen m mulv reverse transcriptase
Nxgen M Mulv Reverse Transcriptase, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase/m+mulv+nxgen+reverse+transcriptase/pm42268475-95-18-22
Average 86 stars, based on 1 article reviews
nxgen m mulv reverse transcriptase - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Quantitative RT-PCR:

Article Title: Comparison of automated and manual mRNA enrichment to automated rRNA depletion for whole-blood RNA-sequencing.
Article Snippet: This was followed by rolling circle replication to create DNA nanoballs (DNB) and these were sequenced on the MGI DNBSEQ-G400RS to generate paired-end sequences of 100 base pairs (bp) using the DNBSEQ-G400RS High-throughput Sequencing Set (FCL PE100) (MGI Tech, Shenzhen, China) according to manufacturer’s instructions. .. Genes from six published TB transcriptomic signatures9,27–30 and three reference genes were translated to RT-qPCR and measured as previously described.25 Briefly, RNA samples were thawed, cDNA synthesised with EpiScript reverse transcriptase (Lucigen, Middleton, WI, USA), and genes of interest pre-amplified using pools of TaqMan gene expression primer-probe assays (Supplementary Table S2; Thermo Fisher Scientific, MA, USA). .. Gene expression (raw cycle threshold, Ct) was subsequently quantified by microfluidic RT-qPCR using Standard BioTools (formerly Fluidigm, CA, USA) 192.24 (192 samples multiplexed with 24 primer-probe assays) Gene Expression chips on a BioMark HD instrument (Standard BioTools).

Reverse Transcription:

Article Title: Comparison of automated and manual mRNA enrichment to automated rRNA depletion for whole-blood RNA-sequencing.
Article Snippet: This was followed by rolling circle replication to create DNA nanoballs (DNB) and these were sequenced on the MGI DNBSEQ-G400RS to generate paired-end sequences of 100 base pairs (bp) using the DNBSEQ-G400RS High-throughput Sequencing Set (FCL PE100) (MGI Tech, Shenzhen, China) according to manufacturer’s instructions. .. Genes from six published TB transcriptomic signatures9,27–30 and three reference genes were translated to RT-qPCR and measured as previously described.25 Briefly, RNA samples were thawed, cDNA synthesised with EpiScript reverse transcriptase (Lucigen, Middleton, WI, USA), and genes of interest pre-amplified using pools of TaqMan gene expression primer-probe assays (Supplementary Table S2; Thermo Fisher Scientific, MA, USA). .. Gene expression (raw cycle threshold, Ct) was subsequently quantified by microfluidic RT-qPCR using Standard BioTools (formerly Fluidigm, CA, USA) 192.24 (192 samples multiplexed with 24 primer-probe assays) Gene Expression chips on a BioMark HD instrument (Standard BioTools).

Article Title: SMARCAL1 is a candidate therapeutic target for ALT-positive tumors
Article Snippet: Total RNA was isolated using the RNeasy kit (#74104, Qiagen) according to the manufacturer’s instructions. .. For real-time quantitative PCR (RT-qPCR) assays, 1 μg of total RNA was reverse-transcribed into cDNA using random hexamer primers (#N8080127, Thermo Fisher Scientific) and MMLV High Performance Reverse Transcriptase kit (#RT80125K, Lucigen). .. An equal amount of cDNA from different samples was mixed with Power SYBR green PCR master mix (#4367659, Thermo Fisher Scientific).

Article Title: Magnetic Field Effects on the Growth and Physiology of Wheat (Triticum aestivum) by Modulation of Photosynthetic Activity and Gene Expression.
Article Snippet: Magnetic field (MF) treatment is a promising approach in boosting plant growth and yield.. Although it is an ecofriendly and safe practice, research on the optimal MF strength, magnetic sensing, and MFinduced molecular response mechanisms in plants is limited.. In this study, we investigated the effects of different MF strengths (20, 40, 120, and 250 milliTeslamT) on key physiological indices (seed germination, tissue growth, physiological indicators, cell membrane integrity, photosynthetic activity, and pigmentation) in wheat.

Article Title: SRC-mediated phosphorylation of UBC9 regulates inflammatory and metabolic signaling in alcohol-associated liver disease
Article Snippet: .. Total RNA was isolated by the Quick-RNA Miniprep Kit (Zymo Research, D4203-A) and reverse transcribed with a cDNA reverse transcription kit (Lucigen, F83902-1) according to the manufacturer’s instructions. .. Quantitative RT-PCR quantified the mRNA levels with iTaq Universal (Bio-Rad, 1724134).

Article Title: Comparison of automated and manual mRNA enrichment to automated rRNA depletion for whole-blood RNA-sequencing
Article Snippet: .. Briefly, RNA samples were thawed, cDNA synthesised with EpiScript reverse transcriptase (Lucigen, Middleton, WI, USA), and genes of interest pre-amplified using pools of TaqMan gene expression primer-probe assays (Supplementary Table ; Thermo Fisher Scientific, MA, USA). .. Gene expression (raw cycle threshold, Ct) was subsequently quantified by microfluidic RT-qPCR using Standard BioTools (formerly Fluidigm, CA, USA) 192.24 (192 samples multiplexed with 24 primer-probe assays) Gene Expression chips on a BioMark HD instrument (Standard BioTools).

Article Title: Calcification in Vascular Smooth Muscle Cells Is Associated with Elevated GCLm and Impaired Contraction: Insights into Osteogenic Transdifferentiation and Therapeutic Approaches.
Article Snippet: Cells were lysed in Trizol reagent (Invitrogen, Carlsbad, CA, USA; Catalog, 15596018) and RNA isolated following the manufacturer’s recommendations. .. 1 μg of total RNA was reverse transcribed using EpiScript RNase H-Reverse Transcriptase (Lucigen, Middleton, WI, USA; Catalog, ERT12925K) and Anchored Oligo dT (IDT, Coralville, IA, USA; Catalog, 51-01-15-09) following the manufacturer’s instructions. .. Gene of interest cDNA was amplified in duplicate using Forget-Me-NotTM EvaGreen® qPCR Master Mix (Biotium, Fremont, CA, USA).

Article Title: DGAT1 regulates keratinocyte proliferation through the modulation of retinoid homeostasis.
Article Snippet: Mean fluorescence intensity within each ROI was measured directly on raw images. real-time qPCR Total RNA was extracted from primary keratinocyte cultures, murine skin biopsies, and human skin biopsies using Fenozol Plus and the Total RNA Zol-Out Kit (A&A Biotechnology). .. RNA concentration and purity were assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized using NxGen M-MulV reverse transcriptase (Lucigen) with random primers (Promega) on a Labcycler thermocycler (SensoQuest). .. Real-time quantitative PCR was performed on a CFX96 thermocycler (Bio-Rad Laboratories) using RT HS PCR Mix SYBR (A&A Biotechnology) and gene-specific primers (Genomed) listed in Table 1.

Gene Expression:

Article Title: Comparison of automated and manual mRNA enrichment to automated rRNA depletion for whole-blood RNA-sequencing.
Article Snippet: This was followed by rolling circle replication to create DNA nanoballs (DNB) and these were sequenced on the MGI DNBSEQ-G400RS to generate paired-end sequences of 100 base pairs (bp) using the DNBSEQ-G400RS High-throughput Sequencing Set (FCL PE100) (MGI Tech, Shenzhen, China) according to manufacturer’s instructions. .. Genes from six published TB transcriptomic signatures9,27–30 and three reference genes were translated to RT-qPCR and measured as previously described.25 Briefly, RNA samples were thawed, cDNA synthesised with EpiScript reverse transcriptase (Lucigen, Middleton, WI, USA), and genes of interest pre-amplified using pools of TaqMan gene expression primer-probe assays (Supplementary Table S2; Thermo Fisher Scientific, MA, USA). .. Gene expression (raw cycle threshold, Ct) was subsequently quantified by microfluidic RT-qPCR using Standard BioTools (formerly Fluidigm, CA, USA) 192.24 (192 samples multiplexed with 24 primer-probe assays) Gene Expression chips on a BioMark HD instrument (Standard BioTools).

Article Title: Comparison of automated and manual mRNA enrichment to automated rRNA depletion for whole-blood RNA-sequencing
Article Snippet: .. Briefly, RNA samples were thawed, cDNA synthesised with EpiScript reverse transcriptase (Lucigen, Middleton, WI, USA), and genes of interest pre-amplified using pools of TaqMan gene expression primer-probe assays (Supplementary Table ; Thermo Fisher Scientific, MA, USA). .. Gene expression (raw cycle threshold, Ct) was subsequently quantified by microfluidic RT-qPCR using Standard BioTools (formerly Fluidigm, CA, USA) 192.24 (192 samples multiplexed with 24 primer-probe assays) Gene Expression chips on a BioMark HD instrument (Standard BioTools).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Peripheral cannabinoid receptor activation attenuates frostbite-induced chronic pain via modulation of TRP channels, neuroinflammation, and autophagy.
Article Snippet: Frostbite injury is a debilitating cold injury encountered in extreme high-altitude and subzero environments, frequently resulting in persistent chronic pain even after tissue healing.. Using our previously validated frostbiteinduced chronic pain model, we further investigated the contribution of neuroimmune, excitatory mechanisms and evaluated the therapeutic efficacy of peripheral cannabinoid receptor activation.. Frostbite produced significant mechanical allodynia, mechanical hyperalgesia, and cold hypersensitivity, along with increased spontaneous ongoing pain behaviors.

SYBR Green Assay:

Article Title: Peripheral cannabinoid receptor activation attenuates frostbite-induced chronic pain via modulation of TRP channels, neuroinflammation, and autophagy.
Article Snippet: Frostbite injury is a debilitating cold injury encountered in extreme high-altitude and subzero environments, frequently resulting in persistent chronic pain even after tissue healing.. Using our previously validated frostbiteinduced chronic pain model, we further investigated the contribution of neuroimmune, excitatory mechanisms and evaluated the therapeutic efficacy of peripheral cannabinoid receptor activation.. Frostbite produced significant mechanical allodynia, mechanical hyperalgesia, and cold hypersensitivity, along with increased spontaneous ongoing pain behaviors.

cDNA Synthesis:

Article Title: Peripheral cannabinoid receptor activation attenuates frostbite-induced chronic pain via modulation of TRP channels, neuroinflammation, and autophagy.
Article Snippet: Frostbite injury is a debilitating cold injury encountered in extreme high-altitude and subzero environments, frequently resulting in persistent chronic pain even after tissue healing.. Using our previously validated frostbiteinduced chronic pain model, we further investigated the contribution of neuroimmune, excitatory mechanisms and evaluated the therapeutic efficacy of peripheral cannabinoid receptor activation.. Frostbite produced significant mechanical allodynia, mechanical hyperalgesia, and cold hypersensitivity, along with increased spontaneous ongoing pain behaviors.

Article Title: Magnetic Field Effects on the Growth and Physiology of Wheat (Triticum aestivum) by Modulation of Photosynthetic Activity and Gene Expression.
Article Snippet: Magnetic field (MF) treatment is a promising approach in boosting plant growth and yield.. Although it is an ecofriendly and safe practice, research on the optimal MF strength, magnetic sensing, and MFinduced molecular response mechanisms in plants is limited.. In this study, we investigated the effects of different MF strengths (20, 40, 120, and 250 milliTeslamT) on key physiological indices (seed germination, tissue growth, physiological indicators, cell membrane integrity, photosynthetic activity, and pigmentation) in wheat.

Real-time Polymerase Chain Reaction:

Article Title: SMARCAL1 is a candidate therapeutic target for ALT-positive tumors
Article Snippet: Total RNA was isolated using the RNeasy kit (#74104, Qiagen) according to the manufacturer’s instructions. .. For real-time quantitative PCR (RT-qPCR) assays, 1 μg of total RNA was reverse-transcribed into cDNA using random hexamer primers (#N8080127, Thermo Fisher Scientific) and MMLV High Performance Reverse Transcriptase kit (#RT80125K, Lucigen). .. An equal amount of cDNA from different samples was mixed with Power SYBR green PCR master mix (#4367659, Thermo Fisher Scientific).

Random Hexamer:

Article Title: SMARCAL1 is a candidate therapeutic target for ALT-positive tumors
Article Snippet: Total RNA was isolated using the RNeasy kit (#74104, Qiagen) according to the manufacturer’s instructions. .. For real-time quantitative PCR (RT-qPCR) assays, 1 μg of total RNA was reverse-transcribed into cDNA using random hexamer primers (#N8080127, Thermo Fisher Scientific) and MMLV High Performance Reverse Transcriptase kit (#RT80125K, Lucigen). .. An equal amount of cDNA from different samples was mixed with Power SYBR green PCR master mix (#4367659, Thermo Fisher Scientific).

Isolation:

Article Title: SRC-mediated phosphorylation of UBC9 regulates inflammatory and metabolic signaling in alcohol-associated liver disease
Article Snippet: .. Total RNA was isolated by the Quick-RNA Miniprep Kit (Zymo Research, D4203-A) and reverse transcribed with a cDNA reverse transcription kit (Lucigen, F83902-1) according to the manufacturer’s instructions. .. Quantitative RT-PCR quantified the mRNA levels with iTaq Universal (Bio-Rad, 1724134).

Concentration Assay:

Article Title: DGAT1 regulates keratinocyte proliferation through the modulation of retinoid homeostasis.
Article Snippet: Mean fluorescence intensity within each ROI was measured directly on raw images. real-time qPCR Total RNA was extracted from primary keratinocyte cultures, murine skin biopsies, and human skin biopsies using Fenozol Plus and the Total RNA Zol-Out Kit (A&A Biotechnology). .. RNA concentration and purity were assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized using NxGen M-MulV reverse transcriptase (Lucigen) with random primers (Promega) on a Labcycler thermocycler (SensoQuest). .. Real-time quantitative PCR was performed on a CFX96 thermocycler (Bio-Rad Laboratories) using RT HS PCR Mix SYBR (A&A Biotechnology) and gene-specific primers (Genomed) listed in Table 1.

Spectrophotometry:

Article Title: DGAT1 regulates keratinocyte proliferation through the modulation of retinoid homeostasis.
Article Snippet: Mean fluorescence intensity within each ROI was measured directly on raw images. real-time qPCR Total RNA was extracted from primary keratinocyte cultures, murine skin biopsies, and human skin biopsies using Fenozol Plus and the Total RNA Zol-Out Kit (A&A Biotechnology). .. RNA concentration and purity were assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized using NxGen M-MulV reverse transcriptase (Lucigen) with random primers (Promega) on a Labcycler thermocycler (SensoQuest). .. Real-time quantitative PCR was performed on a CFX96 thermocycler (Bio-Rad Laboratories) using RT HS PCR Mix SYBR (A&A Biotechnology) and gene-specific primers (Genomed) listed in Table 1.

Synthesized:

Article Title: DGAT1 regulates keratinocyte proliferation through the modulation of retinoid homeostasis.
Article Snippet: Mean fluorescence intensity within each ROI was measured directly on raw images. real-time qPCR Total RNA was extracted from primary keratinocyte cultures, murine skin biopsies, and human skin biopsies using Fenozol Plus and the Total RNA Zol-Out Kit (A&A Biotechnology). .. RNA concentration and purity were assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized using NxGen M-MulV reverse transcriptase (Lucigen) with random primers (Promega) on a Labcycler thermocycler (SensoQuest). .. Real-time quantitative PCR was performed on a CFX96 thermocycler (Bio-Rad Laboratories) using RT HS PCR Mix SYBR (A&A Biotechnology) and gene-specific primers (Genomed) listed in Table 1.



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