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Biouniquer Technology Co Ltd reverse transcriptase (rt) kits
Reverse <t>transcriptase</t> activity of the cultured porcine hepatocytes on different substrata. RT: Reverse transcriptase.
Reverse Transcriptase (Rt) Kits, supplied by Biouniquer Technology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+rt+kits/reverse+transcriptase++rt++kits/pmc03122264-58-25-28
Average 90 stars, based on 1 article reviews
reverse transcriptase (rt) kits - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "Influence of chitosan nanofiber scaffold on porcine endogenous retroviral expression and infectivity in pig hepatocytes"

Article Title: Influence of chitosan nanofiber scaffold on porcine endogenous retroviral expression and infectivity in pig hepatocytes

Journal: World Journal of Gastroenterology : WJG

doi: 10.3748/wjg.v17.i22.2774

Reverse transcriptase activity of the cultured porcine hepatocytes on different substrata. RT: Reverse transcriptase.
Figure Legend Snippet: Reverse transcriptase activity of the cultured porcine hepatocytes on different substrata. RT: Reverse transcriptase.

Techniques Used: Reverse Transcription, Activity Assay, Cell Culture

Related Articles

Reverse Transcription:

Article Title: Dysregulation of the Renin-Angiotensin System and Cardiometabolic Status in Mice Fed a Long-Term High-Fat Diet
Article Snippet: RNA extraction and real-time quantitative polymerase chain reaction (RT-qPCR) of cardiac tissue from C57BL/6J mice Total RNA of the mouse left ventricle tissue samples was extracted using an E.Z.N.A.® Total RNA Kit (R6834) (Omega Biotek Inc., Norcross GA, USA), according to the manufacturer’s instructions. .. The cDNA was obtained by reverse transcription using a SuperScript First-Strand cDNA Synthesis kit (BU-304-01) (Biouniquer Technology, Beijing, China). .. RT-qPCR was performed using SYBR Green I PCR Master Mix with the LightCycle® 96 Instrument (Roche, Basel, Switzerland).

Article Title: Inhibitory effects of 2,3,4',5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells.
Article Snippet: Vascular smooth muscle cells (VSMCs) are the main cellular component in the blood vessel wall, and excessive proliferation of VSMCs plays an important role in the pathogenesis of atherosclerosis, hypertension, and restenosis after angioplasty.. Therefore, the identifi cation of a novel agent that can inhibit VSMCs proliferation and the elucidation of its underlying mechanism will aid in the development of therapeutic strategies aimed at preventing such pathologic vascular conditions.

Article Title: Isolation, purification, structural characterization and immunostimulatory activity of water-soluble polysaccharides from Lepidium meyenii.
Article Snippet: A water-soluble polysaccharide LMP-1 was isolated and purified by ion-exchange chromatography from maca (Lepidium meyenii Walp.).. LMP-1 has a molecular weight of 1.01 104 Da, and is composed of glucose and arabinose with a molar ratio of 7.03:1.08.. Methylation and the 1D and 2D NMR spectroscopy of LMP-1 revealed that it is mainly composed of /4)-a-D-Glcp-(1/, /6)-a-D-Glcp-(1/, /3)-a-DGlcp-(1/, and b-D-Araf-(1/, with branching at O-6 of /4,6)-a-D-Glcp-(1 / .

Article Title: Truncation of Tau selectively facilitates its pathological activities
Article Snippet: Total RNA was extracted from cells by using the HP total RNA kit (Omega Bio-tek, Norcross, GA) according to the manufacturer’s instructions. .. Equal amount of RNA was reverse transcribed to cDNA by using SuperScript 1st Strand cDNA Synthesis Kit (Biouniquer, Nanjing, China). .. Real-time PCR was performed with AceQR qPCR SYBRR Green Master Mix (without ROX) Kit (Vazyme Biotech, Nanjing, China) on a LightCyclerR 96 real-time PCR system (Roche Diagnostics, Basel, Switzerland).

cDNA Synthesis:

Article Title: Dysregulation of the Renin-Angiotensin System and Cardiometabolic Status in Mice Fed a Long-Term High-Fat Diet
Article Snippet: RNA extraction and real-time quantitative polymerase chain reaction (RT-qPCR) of cardiac tissue from C57BL/6J mice Total RNA of the mouse left ventricle tissue samples was extracted using an E.Z.N.A.® Total RNA Kit (R6834) (Omega Biotek Inc., Norcross GA, USA), according to the manufacturer’s instructions. .. The cDNA was obtained by reverse transcription using a SuperScript First-Strand cDNA Synthesis kit (BU-304-01) (Biouniquer Technology, Beijing, China). .. RT-qPCR was performed using SYBR Green I PCR Master Mix with the LightCycle® 96 Instrument (Roche, Basel, Switzerland).

Article Title: Truncation of Tau selectively facilitates its pathological activities
Article Snippet: Total RNA was extracted from cells by using the HP total RNA kit (Omega Bio-tek, Norcross, GA) according to the manufacturer’s instructions. .. Equal amount of RNA was reverse transcribed to cDNA by using SuperScript 1st Strand cDNA Synthesis Kit (Biouniquer, Nanjing, China). .. Real-time PCR was performed with AceQR qPCR SYBRR Green Master Mix (without ROX) Kit (Vazyme Biotech, Nanjing, China) on a LightCyclerR 96 real-time PCR system (Roche Diagnostics, Basel, Switzerland).

Article Title: Qian Yang Yu Yin Granule-containing serum inhibits angiotensin II-induced proliferation, reactive oxygen species production, and inflammation in human mesangial cells via an NADPH oxidase 4-dependent pathway
Article Snippet: RNA concentrations were determined by a microplate reader (BioTek Instruments). .. Total cDNA was synthesized with a cDNA synthesis kit (Biouniquer). .. RT-PCR was performed using an ABI 7700 Prism Sequence Detection System and TaqMan primer probes (Applied Biosystems, Foster City, CA, USA).

Polymerase Chain Reaction:

Article Title: Inhibitory effects of 2,3,4',5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells.
Article Snippet: Vascular smooth muscle cells (VSMCs) are the main cellular component in the blood vessel wall, and excessive proliferation of VSMCs plays an important role in the pathogenesis of atherosclerosis, hypertension, and restenosis after angioplasty.. Therefore, the identifi cation of a novel agent that can inhibit VSMCs proliferation and the elucidation of its underlying mechanism will aid in the development of therapeutic strategies aimed at preventing such pathologic vascular conditions.

RNA Extraction:

Article Title: Inhibitory effects of 2,3,4',5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells.
Article Snippet: Vascular smooth muscle cells (VSMCs) are the main cellular component in the blood vessel wall, and excessive proliferation of VSMCs plays an important role in the pathogenesis of atherosclerosis, hypertension, and restenosis after angioplasty.. Therefore, the identifi cation of a novel agent that can inhibit VSMCs proliferation and the elucidation of its underlying mechanism will aid in the development of therapeutic strategies aimed at preventing such pathologic vascular conditions.

other:

Article Title: miR-185-5p Represses Cells Growth and Metastasis of Osteosarcoma via Targeting Cathepsin E.
Article Snippet: Osteosarcoma (OS) is a malignant bone tumor characterized by poor prognosis due to its regional invasion and early metastasis.. In this study, we aimed to find the role and the underlying mechanism of Cathepsin E (CTSE) in OS growth and metastasis.. We found CTSE is upregulated in metastatic OS, rather than in the primary lesion, as confirmed by RT-qPCR and western blot analysis of clinical OS samples.

Article Title: No transmission of porcine endogenous retrovirus in an acute liver failure model treated by a novel hybrid bioartificial liver containing porcine hepatocytes.
Article Snippet: Author Affiliations: Department of Hepatobiliary Surgery (Han B, Shi XL, Yuan XW, Ren HZ and Ding YT) and Department of Osteology (Qiu Y), the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China; Department of General Surgery, the Third Affiliated Hospital of Suzhou University, Changzhou 213000, China (Zhang Y); and State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210008, China (Gu ZZ)

Article Title: Cordyceps sinensis polysaccharide CPS-2 protects human mesangial cells from PDGF-BB-induced proliferation through the PDGF/ERK and TGF-β1/Smad pathways.
Article Snippet: CPS-2, a Cordyceps sinensis polysaccharide, has been demonstrated to have significant therapeutic activity against chronic renal failure.. However, little is known about the underlying molecular mechanism.. In this study, we found that CPS-2 could inhibit PDGF-BB-induced human mesangial cells (HMCs) proliferation in a dose-dependent manner.

Synthesized:

Article Title: Qian Yang Yu Yin Granule-containing serum inhibits angiotensin II-induced proliferation, reactive oxygen species production, and inflammation in human mesangial cells via an NADPH oxidase 4-dependent pathway
Article Snippet: RNA concentrations were determined by a microplate reader (BioTek Instruments). .. Total cDNA was synthesized with a cDNA synthesis kit (Biouniquer). .. RT-PCR was performed using an ABI 7700 Prism Sequence Detection System and TaqMan primer probes (Applied Biosystems, Foster City, CA, USA).



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